The effect of methamphetamine on biotransformation of ethanol: pilot study

Our results suggest that repeated pre-treatment with methamphetamine led to the decrease of ET levels in time points between minutes 0 and 120 after acute alcohol administration.Výsledek práce dokumentuje, že opakované podání metamfetaminu vede k poklesu hladinu ethanolu v časovém rozmezí 0. až 120. minuta po jeho akutním podání.Our results suggest that repeated pre-treatment with methamphetamine led to the decrease of ET levels in time points between minutes 0 and 120 after acute alcohol administration

Translational research of antidepressants with an example of cannabidiol effects

Despite that not all symptoms of depression can be achieved in animals the translational animal models can mimic the aspects of human depressive disorders in terms of disease symptoms as well as their neurobiological mechanisms. Results of testing the antidepressant drug effects in behavioral animal models of depression are presented. In these models, depressive-like behavior is induced by various stress factors in laboratory animals. However, patients suffering from the Therapeutic Resistant Depression (TRD) do not have high-stress markers. Thus, animals with predisposing factors leading to heightened stress responsiveness as a tool for discovering drugs for human TRD treatment are also presented. Antidepressant treatment effects are reported to be achieved through complex influences on body responses associated to reward, stress, and inflammation, the processes reported to be influenced by activities of the Endocannabinoid System. Cannabidol (the cannabinoid without abuse potential) is reported as an antidepressant agent.</p

Multisource financing in the financial statements of the non-governmental non-profit organization - People for the Ethical Treatment of Animals

The major topic of the bachelor thesis is financial reporting of the non-governmental nonprofit organizations. The theoretical part deals with the characteristics of the non-profit sector and the comparison of the Czech and American environment. The practical part is dedicated to the economic analysis of the american non-profit organization PETA and assessment of its efficiency. Subsequently the American and Czech accounting standards are compared and the differences in financial reporting with the focus on the specifics of the non-profit sector are specified which demonstrates some of the insufficiencies in the Czech accounting legislation

HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals

Although histone acetylation is one of the most widely studied epigenetic modifications, there is still a lack of information regarding how the acetylome is regulated during brain development and pathophysiological processes. We demonstrate that the embryonic brain (E15) is characterized by an increase in H3K9 acetylation as well as decreases in the levels of HDAC1 and HDAC3. Moreover, experimental induction of H3K9 hyperacetylation led to the overexpression of NCAM in the embryonic cortex and depletion of Sox2 in the subventricular ependyma, which mimicked the differentiation processes. Inducing differentiation in HDAC1-deficient mouse ESCs resulted in early H3K9 deacetylation, Sox2 downregulation, and enhanced astrogliogenesis, whereas neuro-differentiation was almost suppressed. Neuro-differentiation of (wt) ESCs was characterized by H3K9 hyperacetylation that was associated with HDAC1 and HDAC3 depletion. Conversely, the hippocampi of schizophrenia-like animals showed H3K9 deacetylation that was regulated by an increase in both HDAC1 and HDAC3. The hippocampi of schizophrenia-like brains that were treated with the cannabinoid receptor-1 inverse antagonist AM251 expressed H3K9ac at the level observed in normal brains. Together, the results indicate that co-regulation of H3K9ac by HDAC1 and HDAC3 is important to both embryonic brain development and neuro-differentiation as well as the pathophysiology of a schizophrenia-like phenotype