11 research outputs found
Chemical analysis and antimicrobial activity of methanol extracts of celandine (Chelidonium majus L.) plants growing in nature and cultured in vitro
Projekat ministarstva br. 143026B i 143041
Chemical analysis and antimicrobial activity of methanol extracts of celandine (Chelidonium majus L.) plants growing in nature and cultured in vitro
Projekat ministarstva br. 143026B i 143041
Enhancement in statistical and image analysis for in situ
Synchrotron-based X-ray microfluorescence (µSXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e.g. single cells and tissues. Elements are mapped and quantified at sub-p.p.m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Košanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by µSXRF
Fitohemijska i anatomska analiza vrste Iris reichenbachii Heuff.
Iris reichenbachii Heuffel (Iridaceae) is an endemic plant species of Balkan penninsula and Romania. Pharmacognostic study of this species are not done till now. The microscopic preparations of the leaves were analyzed and specific orientation of vessels were noticed. Secondary metabolites were analyzed in the samples of leaves collected in different vegetative phase, flowers, fruits and rhizomes. In the samples, g-pyrones and secoiridoids were detected. Among γ-pyrones, a xanthone C-glycoside mangiferin were one of the most abundant compound. It's content varied from 0.5%-8.34% depending of plant organ or vegetative phase of development.Vrsta Iris reichenbachii je endemit Balkanskog poluostrva i Rumunije. Do sada nije farmakognozijski izučavana. U ovom radu je ispitivana anatomska građa lista pri čemu je uočena specifična orijentacija provodnih snopića. Prisustvo sekundarnih metabolita ispitivano je u uzorcima listova, cvetova, plodova i rizoma. Detektovana su jedinjenja g-pironske strukture (ksantoni, flavonoidi) među kojima se po zastupljenosti u uzorcima, kao i po količini ističe ksanton mangiferin. Sadržaj mangiferina varira zavisno od faze razvića i biljnog organa (0.5%-8.34%). Detektovani su i sekoiridoidi od kojih se u značajnijoj količini pojavljuju svercijamarin i genciopikrin
Fitohemijska i anatomska analiza vrste Gentiana dinarica Beck., Gentianaceae
Gentiana dinarica Beck. (Gentianaceae) is a rare plant species growing at carbonate soils in subalpine and alpine regions. Acording to the Flora of Serbia G. dinarica is only distributed in Kosovo. In course of our recent pharmacognostic investigations G. dinarica was found at two localities on the mountain Tara, locality Zvezda (1230 m) and Veliki Kraj (1330 m). Plant material (aerial parts and roots) was collected from June to August 2000-2001. Anatomic and phytochemical analyses were carried out. Preliminary screening of the extracts of the roots and aerial parts of G. dinarica showed the presence of secoiridoids, flavonoids and xanthone compounds. From both extracts three secoiridoids were isolated (swertiamarin, gentiopicrin and sweroside).Vrsta Gentiana dinarica Beck. (Gentianaceae) je retka biljna vrsta koja je u Srbiji, prema Flori Srbije, do sada konstatovana samo na Kosovu. U toku terenskih istraživanja, ovu vrstu smo zabeležili na dva lokaliteta na planini Tari i to lokalitet Zvezda (1230 m) i lokalitet Veliki Kraj (1330 m).Specifičnost anatomske građe korena G. dinarica je slabo odrveneo kislem, i tzv. unutarksilemski floem, odnosno grupice floemskih elemenata u centralnom delu sekundarnog drveta.Preliminarno ispitivanje ekstrakata korena i nadzemnog dela G. dinarica pokazao je prisustvo sekoiridoida, flavonoida i ksantona. Od sekoiridoida, izolovani su svercijamarin, genciopikrin i sverozid
Phytochemical and anatomic study of plant species Gentiana dinarica Beck
Gentiana dinarica Beck. (Gentianaceae) is a rare plant species growing at carbonate soils in subalpine and alpine regions. Acording to the Flora of Serbia G. dinarica is only distributed in Kosovo. In course of our recent pharmacognostic investigations G. dinarica was found at two localities on the mountain Tara, locality Zvezda (1230 m) and Veliki Kraj (1330 m). Plant material (aerial parts and roots) was collected from June to August 2000-2001. Anatomic and phytochemical analyses were carried out. Preliminary screening of the extracts of the roots and aerial parts of G. dinarica showed the presence of secoiridoids, flavonoids and xanthone compounds. From both extracts three secoiridoids were isolated (swertiamarin, gentiopicrin and sweroside).Vrsta Gentiana dinarica Beck. (Gentianaceae) je retka biljna vrsta koja je u Srbiji, prema Flori Srbije, do sada konstatovana samo na Kosovu. U toku terenskih istraživanja, ovu vrstu smo zabeležili na dva lokaliteta na planini Tari i to lokalitet Zvezda (1230 m) i lokalitet Veliki Kraj (1330 m).Specifičnost anatomske građe korena G. dinarica je slabo odrveneo kislem, i tzv. unutarksilemski floem, odnosno grupice floemskih elemenata u centralnom delu sekundarnog drveta.Preliminarno ispitivanje ekstrakata korena i nadzemnog dela G. dinarica pokazao je prisustvo sekoiridoida, flavonoida i ksantona. Od sekoiridoida, izolovani su svercijamarin, genciopikrin i sverozid.nul