Synthesis and characterization of 2-aminoethylphosphonic acid-functionalized graphene quantum dots: biological activity, antioxidant activity and cell viability

317-323A facile, environmentally friendly one-step reaction for the preparation of luminescent N-doped graphene quantum dots (GQDs) involving a hydrothermal reaction between citric acid and 2-aminoethylphosphonic acid has been designed. Graphene quantum dots have been characterized by UV-visible absorption, FTIR spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX) and dynamic light scattering (DLS) techniques. Furthermore, the biological activity of the GQDs has been studied. UV-visible spectroscopy studies of the interactions between the GQDs and calf thymus DNA (CT-DNA) showed that the compound interacts with CT-DNA via intercalative binding. DNA cleavage study showed that the GQDs cleaved DNA oxidatively. In addition, antioxidant activity of N-doped GQDs was measured using the DPPH method. As the concentration of the compound increased, the antioxidant activity also has increased. According to cell viability analyses results, the N-doped GQDs showed cell viability (70%) when the concentration reaches 228 μg/mL for A549, 200 μg/mL for MDA-MB-231 and 140 μg/mL for NIH-3T3 cell lines with 24 h incubation

Cone-beam computed tomography evaluation of impacted and transmigrated mandibular canines: a retrospective study.

Objectives The purpose of this study is to evaluate CBCT images of impacted mandibular canines in detail and to discuss implications for diagnosis and treatment. Methods CBCT images of dental patients applied between December 2015 and December 2017 were evaluated retrospectively.&nbsp;2591 CBCT images, including mandible in their feld of view, were determined and evaluated regarding the presence of mandibular canine impaction. 66 impacted mandibular canines of 58 patients were analyzed in detail regarding their location, morphology, associated pathologies, and their relationship with the neighboring anatomic structures, the role of these factors on transmigration, as well as treatments applied. The Pearson Chi square test was used between the groups. Results Transmigration was observed in 53% of impacted mandibular canines. Teeth with transmigration were localized horizontally, below the apex of adjacent teeth frequently, and had no contact with mandibular cortical bone mostly. 62% of mandibular canines were in contact/proximity with the incisive mandibular canal. The most applied treatment for transmigrated teeth was surgical removal, followed by radiographic monitoring, but orthodontic traction was not preferred in any case. For non-transmigrated teeth, the most applied treatment options were surgical removal, orthodontic traction, and radiographic monitoring, respectively. Autotransplantation was not preferred in any case of impacted teeth. Vertical position of the crown tip (p&lt;0.05) and the presence of deciduous mandibular canines and their resorption showed signifcant relation to transmigration (p&lt;0.05). Conclusion Transmigration is common among impacted mandibular canines. Resorption of adjacent teeth roots was one of the possible complications of impaction and likely contact/proximity of incisive mandibular canal with impacted mandibular canines, which may pose difculties in surgical treatment, can be better detected by CBCT.</p

Synthesis and Characterization of 2-Aminoethylphosphonic Acid-Functionalized Graphene Quantum Dots: Biological Activity, Antioxidant Activity and Cell Viability

A facile, environmentally friendly one-step reaction for the preparation of luminescent N-doped graphene quantum dots (GQDs) involving a hydrothermal reaction between citric acid and 2-aminoethylphosphonic acid was designed. Graphene quantum dots were characterized by UV-Vis, FT-IR spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX) and dynamic light scattering (DLS). Furthermore, the biological activity of the GQDs was studied. UV-Vis spectroscopy studies of the interactions between the GQDs and calf thymus DNA (CT-DNA) showed that the compound interacts with CT-DNA via intercalative binding. DNA cleavage study showed that the GQDs cleaved DNA oxidatively. In addition, antioxidant activity of N-doped GQDs was measured using the DPPH method. As the concentration of the compound increased, the antioxidant activity also increased. According to cell viability analyses results, the N-doped GQDs showed cell viability (70 %) when the concentration reached 228 μg/mL for A549, 200 μg/mL for MDA-MB-231 and 140 μg/mL for NIH-3T3 cell lines with 24 h incubation.

Effectiveness of immediate appointment scheduling in smoking cessation clinics for patients with chronic airway diseases: Preliminary results from a randomized trial

INTRODUCTION Patients with airway diseases who bear the burden of smoking need access to smoking cessation support. We aimed to investigate the impact of immediately scheduled appointments on access to smoking cessation compared with usual care in this patient group. METHODS This multicenter, prospective, randomized, open-label study conducted between November 2022 and June 2023 at pulmonary outpatient clinics. The study included adult patients who were current smokers and diagnosis of asthma, COPD, or bronchiectasis for at least six months. Sequentially randomization was used for the allocation of patients in a 1:1 ratio to two arms: the usual support arm (representing the current standard care procedure) and the immediate support arm (involving intensive brief cessation advice followed by the immediate arrangement of an appointment at the same clinic's smoking cessation service). After one week, both patient groups were contacted by phone to assess their quit attempts and whether they had sought assistance from smoking cessation outpatient clinics (SCCs). RESULTS A total of 397 patients were enrolled in the study, with 199 allocated usual support arm and 198 allocated to the immediate support arm. Within first week, 18.1% of patients in the usual support arm and 77.3% of patients the immediate support arm sought assistance from the smoking cessation (p<0.001). The rate of smokers without an intention to quit was 56.7% usual support arm and 27.7% in the immediate support arm in the first of follow-up. Immediate appointment scheduling was significantly associated with a 13-fold (OR=13.38; 95% CI: 8.00-22.38) increase in referral rates multivariate logistic regression model. CONCLUSIONS Arranging instant appointments has increased access to SCCs times compared to the usual care, this group of patients should be given immediate appointment to SCCs

Post-covid interstitial lung disease: how do we deal with this new entity?

Background: In the postacute phase of coronavirus disease-2019 (COVID-19), survivors may have persistent symptoms, lung function abnormalities, and sequelae lesions on thoracic computed tomography (CT). This new entity has been defined as post-COVID interstitial lung disease (ILD) or residual disease. Aims: To evaluate the characteristics, risk factors and clinical significance of post-COVID ILD. Study design: Multicenter cross-sectional analysis of data from a randomized clinical study. Methods: In this study, patients with persistent respiratory symptoms 3 months after recovery from COVID-19 were evaluated by two pulmonologists and a radiologist. post-COVID ILD was defined as the presence of respiratory symptoms, hypoxemia, restrictive defect on lung function tests, and interstitial changes on follow-up high-resolution computed tomography (HRCT). Results: At the three-month follow-up, 375 patients with post-COVID-19 syndrome were evaluated, and 262 patients were found to have post-COVID ILD. The most prevalent complaints were dyspnea (n = 238, 90.8%), exercise intolerance (n = 166, 63.4%), fatigue (n = 142, 54.2%), and cough (n = 136, 52%). The mean Medical Research Council dyspnea score was 2.1 ± 0.9, oxygen saturation was 92.2 ± 5.9%, and 6-minute walking distance was 360 ± 140 meters. The mean diffusing capacity of the lung for carbon monoxide was 58 ± 21, and the forced vital capacity was 70% ± 19%. Ground glass opacities and fibrotic bands were the most common findings on thoracic HRCT. Fibrosis-like lesions such as interlobular septal thickening and traction bronchiectasis were observed in 38.3% and 27.9% of the patients, respectively. No honeycomb cysts were observed. Active smoking [odds ratio (OR), 1.96; 95% confidence interval (CI), 1.44-2.67), intensive care unit admission during the acute phase (OR, 1.46; 95% CI, 1.1-1.95), need for high-flow nasal oxygen (OR, 1.55; 95% CI, 1.42-1.9) or non-invasive ventilation (OR, 1.31; 95% CI, 0.8-2.07), and elevated serum lactate dehydrogenase levels (OR, 1.23; 95% CI 1.18-1.28) were associated with the development of post-COVID ILD. At the 6-month follow-up, the respiratory symptoms and pulmonary functions had improved spontaneously without any specific treatment in 35 patients (13.4%). The radiological interstitial lesions had spontaneously regressed in 54 patients (20.6%). Conclusion: The co-existence of respiratory symptoms, radiological parenchymal lesions, and pulmonary functional abnormalities which suggest a restrictive ventilatory defect should be defined as post-COVID-19 ILD. However, the term “fibrosis” should be used carefully. Active smoking, severe COVID-19, and elevated lactate dehydrogenase level are the main risk factors of this condition. These post-COVID functional and radiological changes could disappear over time in 20% of the patients

Significance of the Tks4 scaffold protein in bone tissue homeostasis

Abstract The main driver of osteoporosis is an imbalance between bone resorption and formation. The pathogenesis of osteoporosis has also been connected to genetic alterations in key osteogenic factors and dysfunction of bone marrow mesenchymal stem/stromal cells (BM-MSCs). Tks4 (encoded by the Sh3pxd2b gene) is a scaffold protein involved in podosome organization. Homozygous mutational inactivation of Sh3pxd2b causes Frank-ter Haar syndrome (FTHS), a genetic disease that affects bone tissue as well as eye, ear, and heart functions. To date, the role of Tks4 in adult bone homeostasis has not been investigated. Therefore, the aim of this study was to analyze the facial and femoral bone phenotypes of Sh3pxd2b knock-out (KO) mice using micro-CT methods. In addition to the analysis of the Sh3pxd2b-KO mice, the bone microstructure of an FTHS patient was also examined. Macro-examination of skulls from Tks4-deficient mice revealed craniofacial malformations that were very similar to symptoms of the FTHS patient. The femurs of the Sh3pxd2b-KO mice had alterations in the trabecular system and showed signs of osteoporosis, and, similarly, the FTHS patient also showed increased trabecular separation/porosity. The expression levels of the Runx2 and osteocalcin bone formation markers were reduced in the bone and bone marrow of the Sh3pxd2b-KO femurs, respectively. Our recent study demonstrated that Sh3pxd2b-KO BM-MSCs have a reduced ability to differentiate into osteoblast lineage cells; therefore, we concluded that the Tks4 scaffold protein is important for osteoblast formation, and that it likely plays a role in bone cell homeostasis

Adana kenti ve çevresindeki endüstriyel alan kullanımları ve bunların çevreye olan etkilerinin araştırılması

TEZ2144Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 1995.Kaynakça (s.144-152) var.xi, 152 s. ; res. , hrt. ; 30 cm.