Композити графена и наноструктурисаних оксида као компоненте биосензора глукозе и полифенола

Present doctoral dissertation describes the development of novel glucose and polyphenolic index biosensors, based on a combination of graphene nanomaterials and nano-structured metal oxides. An in-depth examination of how mentioned nanomaterials separately influence the electrochemical performances of developed biosensors, as well as their synergic effect, is provided within the scope of this dissertation. In order to obtain desired working electrodes, synthesized nanocomposites were applicated on the surface of screen printed carbon electrodes (SPCEs). Cyclic voltammetry and chronoamperometry were electrochemical methods utilized for testing electrochemical performances of synthetized nanocomposites as well as the final biosensors. Glucose biosensor was based on the modification of graphene nanoribbons (GNR) with Bi2O3 nanoparticles. The synthesized GNR@Bi2O3 nanocomposite was introduced to the surface of SPCE, in order to obtain the working electrode (SPCE/GNR@Bi2O3). Glucose biosensor was prepared by immobilization of glucose-oxidase (GOx), from Aspergillus niger, on the surface of SPCE/GNR@Bi2O3, and followed by coating of the enzyme with Nafion (Naf) solution. Developed SPCE/GNR@Bi2O3/GOx/Naf biosensor has been successfully applied for the quantification of glucose in a honey sample. Polyphenolic index biosensor was constructed by modification of graphene nanoplatelets (GNP) with MnO2 nanoparticles. In order to produce the working electrode (SPCE/GNP@MnO2), SPCE has been modified with GNP@MnO2 nanocomposite. The final polyphenolic index biosensor has been produced by immobilization of the laccase, from Trametes Versicolor (TvL), onto the SPCE/GNP@MnO2 surface, and followed by the application of Naf. Using the developed SPCE/GNP@MnO2/TvL/Naf biosensor, the polyphenolic index in wine was successfully determined.Представљена докторска дисертација описује развој нових биосензора за одређивање глукозе и полифенолног индекса који се заснивају на комбинацији графенских наноматеријала и наноструктурисаних металних оксида. Утицај поменутих наноматеријала на електрохемијске перформансе развијених биосензора, као и њихов синергијски ефекат, је детаљно проучен у оквиру ове дисертације. Синтетисани нанокомпозити су нанети на површину штампаних угљеничних електрода (SPCE) у циљу добијања радних електрода. Циклична волтаметрија и хроноамперометрија су електрохемисјке методе које су коришћене за испитивање електрохемијских перформанси синтетисаних нанокомпозита, као и финалних биосензора. Биосензор за одређивање глукозе се заснивао на модификацији графенских нанотрачица (GNR) са Bi2O3 наночестицама. Синтетисани GNR@Bi2O3 нанокомпозит је нанет на површину SPCE у циљу добијања радне електроде (SPCE/GNR@Bi2O3). Биосензор за одређивање глукозе је припремљен имобилизацијом глукоза-оксидазе (GOx) из Aspergillus niger на површину SPCE/GNR@Bi2O3, што је праћено превлачењем ензима са раствором нафиона (Naf). Развијени SPCE/GNR@Bi2O3/GOx/Naf биосензор је успешно примењен за квантификацију глукозе у узорку меда. Биосензор за одређивање полифенолног индекса се базирао на модификацији графенских наноплочица (GNP) са MnO2 наночестицама. SPCE је модификована са GNP@MnO2 нанокомпозитом у циљу конструкције радне електроде (SPCE/GNP@MnO2). Биосензор за одређивање полифенолног индекса је конструисан након имобилизације лаказе из Trametes Versicolor (TvL) на површину SPCE/GNP@MnO2, након чега је уследило наношење Naf раствора. Полифенолни индекс у узорцима вина је одређен користећи развијени SPCE/GNP@MnO2/TvL/Naf биосензор

Supplementary data for the article: Vukojević, V.; Durđić, S.; Mutić, J. Accumulation of U, Th, Pb, V, Rb, and Ag in Wild Mushrooms Macrolepiota Procera (Scop.) Singer from Goc, Serbia. Environ. Sci. Pollut. Res. 2019, 26 (13), 13147–13158. https://doi.org/10.1007/s11356-019-04723-z

Supplementary material for: [https://link.springer.com/article/10.1007%2Fs11356-019-04723-z]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3283

Supplementary data for the article: Stanković, V.; Đurđić, S.; Ognjanović, M.; Mutić, J.; Kalcher, K.; Stanković, D. M. A Novel Nonenzymatic Hydrogen Peroxide Amperometric Sensor Based on AgNp@GNR Nanocomposites Modified Screen-Printed Carbon Electrode. Journal of Electroanalytical Chemistry 2020, 876, 114487. https://doi.org/10.1016/j.jelechem.2020.114487

Supplementary material for: [https://doi.org/10.1016/j.jelechem.2020.114487]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/4033

Supplementary material for the article: Stefanović, V.; Trifković, J.; Djurdjić, S.; Vukojević, V.; Tešić, Ž.; Mutić, J. Study of Silver, Selenium and Arsenic Concentration in Wild Edible Mushroom Macrolepiota Procera, Health Benefit and Risk. Environmental Science and Pollution Research 2016, 23 (21), 22084–22098. https://doi.org/10.1007/s11356-016-7450-2

Supplementary material for: [https://doi.org/10.1007/s11356-016-7450-2]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2348

Supplementary material for the article: Vukojević, V.; Djurdjić, S.; Ognjanović, M.; Antić, B.; Kalcher, K.; Mutić, J.; Stanković, D. M. RuO2/Graphene Nanoribbon Composite Supported on Screen Printed Electrode with Enhanced Electrocatalytic Performances toward Ethanol and NADH Biosensing. Biosensors and Bioelectronics 2018, 117, 392–397. https://doi.org/10.1016/j.bios.2018.06.038

Supplementary material for: [https://doi.org/10.1016/j.bios.2018.06.038]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2204]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2985

Supplementary data for the article: Stanković, V.; Đurđić, S.; Ognjanović, M.; Antić, B.; Kalcher, K.; Mutić, J.; Stanković, D. M. Anti-Human Albumin Monoclonal Antibody Immobilized on EDC-NHS Functionalized Carboxylic Graphene/AuNPs Composite as Promising Electrochemical HSA Immunosensor. Journal of Electroanalytical Chemistry 2020, 860. https://doi.org/10.1016/j.jelechem.2020.113928

Supplementary material for: [https://doi.org/10.1016/j.jelechem.2020.113928]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3881]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3891

Supplementary data for the article: Stanković, V.; Đurđić, S.; Ognjanović, M.; Antić, B.; Kalcher, K.; Mutić, J.; Stanković, D. M. Anti-Human Albumin Monoclonal Antibody Immobilized on EDC-NHS Functionalized Carboxylic Graphene/AuNPs Composite as Promising Electrochemical HSA Immunosensor. Journal of Electroanalytical Chemistry 2020, 860. https://doi.org/10.1016/j.jelechem.2020.113928

Supplementary material for: [https://doi.org/10.1016/j.jelechem.2020.113928]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3881]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3891

Supplementary data for article: Đurđić, S. Z.; Sredojević, M.; Trifković, J.; Vukojević, V.; Natić, M.; Tešić, Ž. L.; Mutić, J. Elemental Composition as a Tool for the Assessment of Type, Seasonal Variability, and Geographical Origin of Wine and Its Contribution to Daily Elemental Intake. RSC Advances 2017, 7 (4), 2151–2162. https://doi.org/10.1039/c6ra25105f

Supplementary material for: [https://doi.org/10.1039/c6ra25105f]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2397

Elemental composition as a tool for the assessment of type, seasonal variability, and geographical origin of wine and its contribution to daily elemental intake

The elemental profiles of 63 red and white wine samples from four different regions in Serbia were investigated. Twenty-one elements were analysed (Ca, Mg, Na, K, Fe, Mn, Cu, Zn, Co, Se, Cr, V, Ni, Cd, As, Al, Sb, Pb, Ba, Rb, and Be) by inductively coupled plasma quadrupole mass spectrometry (ICP-Q-MS) and inductively coupled plasma with optical emission spectrometry (ICP-OES). A pattern recognition method was applied in order to classify and differentiate type, seasonal variability, and geographical origin of the wine. Dietary mineral intake for elements was calculated in order to assess their contribution to daily intake. The most important descriptors for discrimination among red and white wine samples were Be, Al, Rb, Mg, K, Cu, Mn, and Na, in descending order. The variables Cd, Pb, As, Sb, V, Na, K, and Zn have the highest influence on vintage-to-vintage classification of red wines. Furthermore, the model revealed the existence of three groups of descriptors for different regions of production. All obtained statistical models confirmed that data from the elemental content of wine samples could be used for accurate prediction of wine type, seasonal variability, and regional origin.Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2979

Supplementary data for article: Đurđić, S.; Vukojević, V.; Vlahović, F.; Ognjanović, M.; Švorc; Kalcher, K.; Mutić, J.; Stanković, D. M. Application of Bismuth (III) Oxide Decorated Graphene Nanoribbons for Enzymatic Glucose Biosensing. Journal of Electroanalytical Chemistry 2019, 850. https://doi.org/10.1016/j.jelechem.2019.113400

Supplementary material for: [https://doi.org/10.1016/j.jelechem.2019.113400]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3751