11 research outputs found
Molecular diagnosis of Toxoplasma gondii and Neospora caninum in brain tissues of some wild birds
Çiftlik hayvanlarının ekonomik öneme sahip protozoonlarından Toxoplasma gondii ve Neospora caninum un ara konak yabani kuşlardaki moleküler tanısı hakkında az sayıda araştırma bulunmaktadır. Türkiyede bu parazitlerin yabani kuşların beyin dokularındaki moleküler prevalansı bilinmemektedir. Bu çalışmada, Türkiyenin iki farklı ekocoğrafyasında bulunan 20 yabani kuş türüne ait 101 adet beyin dokusunda T. gondii ve N. caninum un varlığı polimeraz zincir reaksiyonu (PZR) metodu ile araştırılmıştır. Araştırma sonucunda yabani kuşların beyin dokularında T. gondii %9, N. caninum %14, her iki türe aynı anda rastlanma oranı ise %4 olarak tespit edilmiştir. Elde edilen verilere Ki kare testi uygulanmıştır. Sonuç olarak bu çalışmada T. gondiinin Corvus corone (Gri leş kargası), Melanitta fusca (Kadife ördek), Aquila heliaca (Şah kartal), Aquila pomarina (Küçük orman kartalı), Buteo rufinus (Kızıl şahin), Accipiter nisus (Bayağı atmaca), Strix aluco (Alaca baykuş)un beyin dokularında ve N. caninumun Larus genei (İnce gagalı martı), Corvus corone, Melanitta fusca, Anas clypeata (Bayağı kaşık gaga), Perdix perdix (Çil keklik), Aquila heliaca ve Buteo rufinus un beyin dokularında PZR metoduyla dünyada ilk tespiti yapılmıştır. Araştırma Türkiyedeki yaban kuşlarında T. gondii ve N. caninum un moleküler tanısı amacıyla yapılan ilk çalışmadır.There are limited molecular studies about Toxoplasma gondii and Neospora caninum which are economically important livestock protozoons in wild birds investigated by polymerase chain reaction (PCR) method. Molecular prevalance of both parasites in brain tissues of wild birds in Turkey is unknown. Prevalance of T. gondii was 7%, N. caninum was 14% and mix infection was found 4% in brain tissues of 101 wild birds under 20 species from two diferent regions of Turkey. The chi-square test has been applied to the acquired data. This is the first molecular biologic investigation for the aim of PCR diagnosis of T. gondii in brain tissues of Corvus corone, Melanitta fusca, Aquila heliaca, Aquila pomarina, Buteo rufinus, Accipiter nisus, Strix aluco and N. caninum in brain tissues of Larus genei, Corvus corone, Melanitta fusca, Anas clypeata, Perdix perdix, Aquila heliaca, Buteo rufinus in the world. This also is the first molecular diagnostic investigation of T. gondii and N. caninum in brain tissues of wild birds in Turkey
Molecular Survey of Toxoplasma gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye
Toxoplasma gondii, an obligatory intracellular protozoan parasite, can infect a wide range of warm-blooded animals, including livestock species. T. gondii is a zoonotic protozoan parasite that affects both humans and other warm-blooded animals. The aim of this study was to detect T. gondii by using PCR in the brain tissues of 60 aborted sheep fetuses from the Iğdır Province in Türkiye. For this purpose, 60 brain tissue samples of sheep were collected within the lambing seasons of 2023 in Iğdır, Türkiye. The DNA extraction was performed using the PureLink™ Genomic DNA Mini Kit from brain samples. The PCR was performed with the appropriate primers from the obtained DNA samples. T. gondii was found in the brain (16.6%) samples of aborted sheep fetuses. According to the present study, T. gondii infection can be one of the causes of fetus abortion of sheep in Iğdır province, Türkiye. This result emphasizes the need for vigilance and preventive measures in managing this potential public and animal health concerns
Intersection problem for Droms RAAGs
We solve the subgroup intersection problem (SIP) for any RAAG G of Droms type
(i.e., with defining graph not containing induced squares or paths of length
3): there is an algorithm which, given finite sets of generators for two
subgroups H,K of G, decides whether is finitely generated or not,
and, in the affirmative case, it computes a set of generators for .
Taking advantage of the recursive characterization of Droms groups, the proof
consists in separately showing that the solvability of SIP passes through free
products, and through direct products with free-abelian groups. We note that
most of RAAGs are not Howson, and many (e.g. F_2 x F_2) even have unsolvable
SIP.Comment: 33 pages, 12 figures (revised following the referee's suggestions
Molecular Survey of the Toxoplasma gondii and Neospora caninum in brain tissue of aborted fetuses of Morkaraman sheep in Muş, Türkiye
Toxoplasma gondii and Neospora caninum are obligate intracellular protozoan parasites that can affect different warm-blooded species worldwide. In this study, it was aimed to detect T. gondii and N. caninum using PCR method in brain tissues of aborted sheep fetuses. Brain specimens were collected from 50 Morkaraman sheep fetuses that had undergone abortion at various stages of pregnancy, within the lambing seasons of 2023 in Muş. Approximately 1 cm³ of brain tissue from the right cerebral hemisphere was excised and subsequently frozen at -20°C for DNA extraction. DNA extraction and PCR amplification were then performed. As a result of this study, 11 (22%) of 50 brain tissues were positive. All brain samples examined in this study were negative for Neospora caninum. Based on the results of this study, it is possible to say that T. gondii is an important abortion agent in sheep in this region. Although N. caninum was not detected in this study, larger scale studies are recommended. Moreover, this study provides important information to breeders and veterinarians in the field in the evaluation and management of abortion
Generic amplification and next generation sequencing reveal Crimean-Congo hemorrhagic fever virus AP92-like strain and distinct tick phleboviruses in Anatolia, Turkey
Van İlinde Dışkı Muayenesine Göre Sokak Köpeklerinde Görülen Mide-Bağırsak Parazitleri
msufbdThis study was carried out in order to investigate the contamination of helmint eggs, protozoon oocyst and cyst of street dogs’ feces in range of Van, and its signifiance on public health. In this study extent, 50 feces material has been taken in feces containers separately. The label swith the information of where and when the material was taken, have been sticked an fecal containers. Feces materials have been examined with flotation method using satured salted water, and examined nativ method using physiological saline solution. Eggs, cyst and oocysts that found in fecal, have been diagnosed with related literature. At the end of the study, in examined 50 excrement pieces, 39 (%78) parasitory infection has been identified via flotation methodand 30 (%60) parasitory infection has been identified via nativ method. On commited examinations, Toxocara canis, Toxascaris leonina, Uncinaria sp. And Taenia sp. eggs, Isospora sp. and Eimeria sp. oocysts, Giardia sp. Entomoeba sp. and Blastocystis sp. cyst have been diagnosed. It is conclued that these identified parasites threaten public health and it is compulsory that these to take precaution. Bu çalışma Van Büyükşehir Belediyesi sınırları içerisinde bulunan sokak köpeklerine ait dışkıların helmint yumurtaları ve protozoon ookistleri ile kontaminasyon durumunu ve halk sağlığı açısından önemini araştırmak amacıyla gerçekleştirildi. Çalışma kapsamında 50 adet köpeğin dışkı materyali üzerinde tarih yazılı dışkı kaplarına toplandı. Dışkı materyalleri doymuş tuzlu su kullanılarak flotasyon yöntemi ve serum fizyolojik kullanılarak nativ yöntem ile incelendi. Dışkı bakısında bulunan yumurta, kist ve ookistler ilgili literatürler ışığında teşhis edildi. Çalışma sonunda incelemesi yapılan 50 dışkı örneğinin flotasyon yöntemi ile 39 (%78) ve nativ yöntem ile 30 (%60)'unda paraziter enfeksiyon tespit edildi. Yapılan dışkı muayenelerinde Toxocara canis, Toxascaris leonina, Uncinaria sp. ve Taenia sp.yumurtaları, Isospora sp.ve Eimeria sp. ookistleri, Giardia sp., Entamoeba sp. ve Blastocystis sp.kistleri tespit edildi.Tespit edilen bu parazitlerin halk sağlığını tehdit ettiği ve önlem alınmasının zorunlu olduğu kanısına varılmıştır. 31535
Diagnostic and Prognostic Value of Procalcitonin Levels in the Sheep with Natural Babesiosis Before and After Treatment
Bu çalışma Babesia ovis ile doğal enfekte koyunlarda prokalsitonin (PCT), C reaktif protein (CRP), Nitrik oksit (NO) ve Adenozin deaminaz (ADA) düzeylerinin hastalığın diagnosunda ve prognosundaki önemini ortaya koymak amacıyla yapıldı. Klinik ve parazitolojik olarak babesiosis teşhisi konulmuş 30 adet koyun iki gruba ayrılmış birinci gruba sadece imidokarp dipropionat ikinci gruba ise imidokarp dipropionat ve fluniksin meglumin ile tedavi edilmiştir. 15 adet sağlıklı koyunda kontrol grubunu oluşturmuştur. Tedaviden 7 gün sonra babesiosisli gruplardan tekrar kan örnekleri alınarak tedaviye yanıtlarına bakılmıştır. Tedavi sonucu tüm hayvanların tedaviye olumlu cevap vererek iyileştikleri tespit edildi. Babesiosisli koyunlarda serum PCT, CRP, NO ve ADA düzeylerinin sağlıklı koyunlara göre arttığı ve istatistiksel olarak önemli olduğu tespit edildi. Babesiosisli koyunlarda tedaviden önce (T.Ö) ve tedaviden sonra (T.S.) PCT, CRP, NO ve ADA düzeyleri karşılaştırıldığında tedaviden sonra bu parametrelerin düzeylerinin azaldığı tespit edilmiş ikinci grupta ADA hariç istatistiksel olarak önemli oldukları tespit edilmiştir. Sonuç olarak babesiosili koyunlarda PCT, CRP, NO ve ADA düzeylerinin ölçülmesinin hastalığın diagnozunda ve prognozunda klinik muayeneyle birlikte değerlendirildiğinde faydalı olabileceği kanaatine varılmıştır.This study was carried out to reveal the importance of procalcitonin (PCT), C reactive protein (CRP), nitric oxide (NO) and adenosine deaminase (ADA) levels in the diagnosis and prognosis of the disease in natural infected sheep with Babesia ovis. Thirty sheep diagnosised clinically and parasitologically as babesiosis was allocated to two groups and the first group was treated only with imidocarp dipropionate and the second group with imidocarp dipropionate and flunixine meglumin. Fifteen healthy sheep were also served as control group. Seven days after the treatment, blood samples were collected again from the babesiosis group sheep and the treatment responses were assessed. At the end of treatment, all animals were found to have responded positively to the treatment. Serum PCT, CRP, NO and ADA levels were higher in Babesiosis sheep compared to that in healthy sheep and the difference was found to be statistically significant. When PCT, CRP, NO and ADA levels before treatment (B.T.) and after treatment (A.T.) in Babesiosis sheep were compared, the levels of these parameters except for ADA in the second group was determined to decrease statistically after the treatment. As a result, it has been concluded that the measurements of PCT, CRP, NO and ADA levels in babesiosis sheep may be useful for the diagnosis and prognosis of the disease when assessed in association with the clinical examination
Molecular diagnosis of Toxoplasma gondii and Neospora caninum in brain tissues of some wild birds
Çiftlik hayvanlarının ekonomik öneme sahip protozoonlarından Toxoplasma gondii ve Neospora caninum ’un ara konak yabani kuşlardaki moleküler tanısı hakkında az sayıda araştırma bulunmaktadır. Türkiye’de bu parazitlerin yabani kuşların beyin dokularındaki moleküler prevalansı bilinmemektedir. Bu çalışmada, Türkiye’nin iki farklı ekocoğrafyasında bulunan 20 yabani kuş türüne ait 101 adet beyin dokusunda T. gondii ve N. caninum ’un varlığı polimeraz zincir reaksiyonu (PZR) metodu ile araştırılmıştır. Araştırma sonucunda yabani kuşların beyin dokularında T. gondii %9, N. caninum %14, her iki türe aynı anda rastlanma oranı ise %4 olarak tespit edilmiştir. Elde edilen verilere Ki kare testi uygulanmıştır. Sonuç olarak bu çalışmada T. gondii’nin Corvus corone (Gri leş kargası), Melanitta fusca (Kadife ördek), Aquila heliaca (Şah kartal), Aquila pomarina (Küçük orman kartalı), Buteo rufinus (Kızıl şahin), Accipiter nisus (Bayağı atmaca), Strix aluco (Alaca baykuş)’un beyin dokularında ve N. caninum’un Larus genei (İnce gagalı martı), Corvus corone, Melanitta fusca, Anas clypeata (Bayağı kaşık gaga), Perdix perdix (Çil keklik), Aquila heliaca ve Buteo rufinus ’un beyin dokularında PZR metoduyla dünya’da ilk tespiti yapılmıştır. Araştırma Türkiye’deki yaban kuşlarında T. gondii ve N. caninum ’un moleküler tanısı amacıyla yapılan ilk çalışmadır.There are limited molecular studies about Toxoplasma gondii and Neospora caninum which are economically important livestock protozoons in wild birds investigated by polymerase chain reaction (PCR) method. Molecular prevalance of both parasites in brain tissues of wild birds in Turkey is unknown. Prevalance of T. gondii was 7%, N. caninum was 14% and mix infection was found 4% in brain tissues of 101 wild birds under 20 species from two diferent regions of Turkey. The chi-square test has been applied to the acquired data. This is the first molecular biologic investigation for the aim of PCR diagnosis of T. gondii in brain tissues of Corvus corone, Melanitta fusca, Aquila heliaca, Aquila pomarina, Buteo rufinus, Accipiter nisus, Strix aluco and N. caninum in brain tissues of Larus genei, Corvus corone, Melanitta fusca, Anas clypeata, Perdix perdix, Aquila heliaca, Buteo rufinus in the world. This also is the first molecular diagnostic investigation of T. gondii and N. caninum in brain tissues of wild birds in Turkey
seroprevalance of anaplasmosis in cattle in Van, Muş, Siirt and Diyarbakır Provinces
Rikettsiales dizisi, Anaplasmatacae ailesindeki Anaplasma türlerinin meydana getirdiği anaplasmosis, tropik ve subtropik iklim bölgelerindeki memeli hayvanlarda görülen enfeksiyöz bir hastalıktır. Sığır anaplasmosisi genellikle Anaplasma marginale ile ilişkilendirilir ve bu hastalık hem kan emici sinekler ile mekanik hem de keneler ile biyolojik yolla nakledilir. Bu çalışma Van, Muş, Siirt ve Diyarbakır İllerinde sığırlarda Anaplasma spp. seroprevalansının araştırılması amacıyla yapılmıştır. Serum örnekleri ticari cELISA kiti ile Anaplasma’ya karşı gelişen antikorlar yönünden analiz edilmiştir. Çalışma sonucunda incelenen 182 sığırın 52’sinde (%28,6) Anaplasma spp. antikorları bakımından seropozitiflik saptanmıştır. Dişi sığırlarda seroprevalans %29.3, erkeklerde ise %27.1 olarak belirlenmiş ve bu farklılık istatistiksel açıdan önemsiz bulunmuştur. İstatiksel olarak sığırlarda yaş gruplarına göre farklı seropozitiflik saptanmış olup, en yüksek oran 3-5 (%45.1) yaş arasındaki hayvanlarda bulunmuştur. Ayrıca çalışma merkezleri arasında en yüksek seropozitiflik %78.7 oranı ile Siirt ilinde belirlenmiştir. Sonuç olarak, Van, Muş, Siirt ve Diyarbakır illerinde sığırlarda subklinik ve kronik Anaplasma enfeksiyonlarının varlığı ortaya konulmuştur.Anaplasmosis, caused by the genus Anaplasma related to the family Anaplasmataceae the order Rickettsiales, is an infectious disease occurs in mammals in tropical and subtropical climatic regions. Bovine anaplasmosis is usually associated with Anaplasma marginale infection in cattle and its can be transmitted both mechanically by biting flies or and biologically by ticks. The purpose of this study was to investigate the presence of Anaplasma spp. in cattle in Van, Mus, Siirt and Diyabakir provinces. cELISA was used to detect specific anti-Anaplasma spp. antibodies in the serum samples. 52 (28.6%) of the 182 asymptomatic cattle were seropositive against Anaplasma. The prevalence of anaplasmosis in female and male cattle was found as 29.3% and 27.3%, respectively and this difference was not found significant. Seropositive rate was statistically differ among the age groups of cattle and the highest seropositive rate was found in 3-5 years. Moreover, the highest seropositive rate of study sites was determined in Siirt as 78.7%. As a result, this is serologic survey for subclinical and chronic Anaplasma spp. infections performed on cattle in Van, Mus, Siirt and Diyabakir province
MOLECULAR DETECTION OF MYCOPLASMA HAEMOCANIS AND CANDIDATUS MYCOPLASMA HAEMATOPARVUM IN RHIPICEPHALUS SANGUINE US TICK SPECIES COLLECTED FROM DOGS IN ADANA PROVINCE OF TURKEY
Mlycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp) arc hemo-tropic mycoplasma species known to be specific to dogs. Rhipicephalus sanguineus (sensu lam) ticks have been suggested to bc the main vector in transmission; but a clear relationship between tick infestation and the disease has yet to be established under field conditions. This study aimed to investigate the presence of Mhc and CMhp in R. sanguineus (s.1.) s.ticks that arc considered to be a possible vector for hemoplasma infections, Using the species -specific polymerase chain reaction (PCR) test, the presence of Mhc. and CMhp was investigated in total of 312 archived adult tick samples collected from 63 pet dogs kept in the municipal dog shelter of Adana province located in the Mediterranean region of Turkey. Although R. sanguineus (s.1.) ticks tested negative for Mhc, 2.56% (n = 8) were positive for CMhp. There was a statistically significant difference in the Mhc and CMhp detection rates in R. sanguineus (s.1.) ticks (2.56%; 95% CI: 0.81 - 4.31; P < 0.05). To our knowledge, this is the first study to reveal the presence of CAlhp in R. sanguineus (s.l.) ticks collected from dogs in Turkey