Dostu avukat Halit Çelenk, Cemil Gezmiş'in acılı gününü yazdı:'Rahat uyumak senin hakkın'

Taha Toros Arşivi, Dosya No: 27/A-Deniz GezmişUnutma İstanbul projesi İstanbul Kalkınma Ajansı'nın 2016 yılı "Yenilikçi ve Yaratıcı İstanbul Mali Destek Programı" kapsamında desteklenmiştir. Proje No: TR10/16/YNY/010

Alın Yazısı

Taha Toros Arşivi, Dosya No: 34-Nahit Sırrı Örikİstanbul Kalkınma Ajansı (TR10/14/YEN/0033) İstanbul Development Agency (TR10/14/YEN/0033

Isolation and characterization of Bacillus thuringiensis from olive tree-related habitats

Thesis (Master)--Izmir Institute of Technology, Biotechnology, Izmir, 2005Text in English; Abstract: Turkish and Englishxii,115 leavesAbstract:Bacillus thuringiensis (commonlyreferred to as Bt) is a Gram-positive, sporeforming soil bacterium that produces insecticidal crystal proteins during sporulation.These crystals are referred as Bt toxins or -endotoxins. The most important characteristics of the toxins are their insecticidalactivity against many insects. Since their insecticidal potential has been discovered,it has been produced commercially and used as microbial pesticides all over the world.Therefore, the aim of this study was to isolate Btfrom olive tree-related habitats, and to determine the phenotypic andisolate Btfrom olive tree-related habitats, and to determine the phenotypic and genotypic characteristics of the isolates. To accomplish this purpose, 240 samples were collected in the Aegean Region. The phase-contrast microscopy results showed the presence of crystals in 54 environmental samples, corresponding to 100 Bt isolates. The crystal morphologies were spherical, bipyramidal, cuboidal, irregular pointed, and irregular shaped. The greatest proportion of samples yielding this organism was from the soil. The remaining were from olive leaf residue, green olive leaves, animal faeces,dust samples, and olive pomace. The isolates were characterized on the basis of biochemical characters, cry andcyt gene content, plasmid profiling, 16S-ITS rDNA RFLP.Biochemical tests included protease (caseinase and gelatinase), lecithinase, amylase, nuclease, urease, esculinase, arginine dihydrolyse activity; fermentation of sucrose, salicin, mannose, cellobiose, and maltose, production of acetyl-methyl carbinol, methyl red reaction. Polymerase chain reaction (PCR) has been applied for the identification of cry1, cry2, cry4, cry9, cry11, cry13, cyt1, and cyt2 genes. 68% of the isolates amplified cry1 gene; 57% amplified cry4; 20% amplified cry11; 26% amplified cry9; 20% amplified cry2 genes, andnone of the isolates harbored cry13 gene. Cyt1gene was found in 40% of the isolates while cyt2 gene was present in 80% of the isolates. The most abundant genotype of cry genes was cry1 and cry4. Most of the isolates(58%) possessed more than one cry gene. In addition, different combinations of cry andcytgenes were obtained. Plasmid profiling showed the presence of plasmids in all isolatesand the number of plasmids was usually morethan one. Also, the discrimation effect of 16S-ITS rDNA RFLP was tested to differentiate certain isolates and reference strains, which showed similar biochemical characteristics

Motivation Affects Sports and Life Skills in Physical Disabled People

In this study, it has been tried to determine the motivation of participation in sports and the effects of sports on their lifestyles of different physically disabled individuals who do sports and those who do not, and the differences between the types of disabilities were examined. A total of 732 physically disabled individuals including 327 people who do sports and 405 people who do not do sports were included in the study. Personal Information Form, Physical Activity Participation Motivation Scale (PAPMS) and the Effect of Sports on Life Skills Scale (SLSS) were used as data collection tools. There is a statistically significant difference between those who do sports and those who do not in total and all sub-parameters in PAPMS and SLSS (p <0.05). When the total scores and sub-dimensions of the participants are examined, there is a positive correlation between PAPMS and SLSS (p <0.05). When the responses of the participants to FAPMS and SLSS are compared according to the type of disability, there is a statistically significant difference between those who do sports and those who do not in the total and sub-dimensions of Orthopedic, Visually and hearing impaired individuals (p <0.05). As a result, it shows that sports have a positive role in the motivation of participating in physical activities and contribute to life skills on disabled individuals who do sports. It is thought that sports will have a positive effect not only on their participation in physical activities, but also in many cognitive learning processes due to the increase in motivation of people with disabilities and help the disabled person to become more active and accepted in the society

Overview of current fluorine-free remineralization materials and methods as an alternative to topical fluoride: An up-to-date

Dental caries is a common public health problem around the world, and it has been aimed to solve this health problem with many different materials and methods from the past to the present. With the demineralization process occurring in the tooth’s hard tissues, mineral loss occurs and initial caries lesions and cavitations due to caries occur. On the other hand, the remineralization process can be defined as the removal of existing minerals and hard tissue loss with ions in saliva, newly developed or existing materials,s and methods. The success of fluoride in remineralization has been proven by many literature data and is accepted as the gold standard. Although the use of fluoride in preventive dentistry is the gold standard; Due to the possible side effects and the refusal of fluoride by parents and healthcare professionals, researchers have sought new remineralization materials and methods that can provide remineralization, increase the effectiveness of fluoride or be an alternative to fluoride, with the aim of reducing the fluoride concentrations used. In clinical applications, as an alternative to flora, mineral and ion technologies, sugar alcohols, plant-derived products, bioactive materials, nanotechnological products, calcium, and phosphate-derived other products contribute to the remineralization process and are among the current remineralization materials and methods. In this review, up-to-date information on fluorine-free remineralization materials, mechanisms of action, and clinical applications of new methods and technologies were examined in order to evaluate them in line with the results of scientific studies, and it was aimed to present the studies on this subject

Evaluation of bioactivity of pomegranate fruit extract against Alicyclobacillus acidoterrestris DSM 3922 vegetative cells and spores in apple juice

This research evaluated the antimicrobial activity of commercial pomegranate extract (POMELLA®, PE) against Alicyclobacillus acidoterrestris vegetative cells and spores (approximately 105 log CFU/mL) in apple juice (pH 3.82, °Brix 11.3) during storage at 37°C. After 240h, the cell counts were reduced from the initial log count (CFU/mL) by 2.84, 3.26, 3.32, 3.46 and 3.56 in the apple juice with PE at the concentrations of 2.5, 5, 10, 20 and 40μg/mL, respectively. On the other hand, counts of the control reached 7.36 log CFU/mL after 24h. The Weibull model satisfactorily described the survival curves of cell inactivation kinetics (R2 > 0.983). While the growth of all spores obtained from different sporulation media (potato dextrose agar, malt extract agar, Bacillus acidoterrestris agar, and Bacillus acidocaldarius agar) was inhibited in the apple juice with PE (2.5-40μg/mL), the control spores increased by 1.9-2.2 log CFU/mL after 336h. Based on the scanning electron microscopy (SEM) imaging, vegetative cells indicated substantial damage and spore germination was inhibited in the apple juice with PE. The results showed that PE can have possible uses as a natural antimicrobial to control the growth of A.acidoterrestris vegetative cells and spore germination in the apple juice. © 2015 Elsevier Ltd

The effect of sporulation medium on Alicyclobacillus acidoterrestris guaiacol production in apple juice

The present study evaluated the effect of sporulation medium on guaiacol formation from vanillin and vanillic acid by Alicyclobacillus acidoterrestris DSM 3922 in the reconstituted apple juice (pH 3.82, °Brix 11.3). For sporulation, potato dextrose agar and Bacillus acidoterrestris agar were used. After heat-activation, spores were turned into vegetative cells and inoculated into juice samples to a final concentration of 103 or 105 CFU/mL. Samples were incubated at 37 °C for 264 h and guaiacol concentration was determined using peroxidase enzyme colourimetric assay. Based on the results, the conversion of vanillic acid into guaiacol was faster than that of vanillin among both cell suspensions. Also, there were no significant differences among any of the samples inoculated into apple juice spiked with vanillin at the end of the incubation period (P > 0.05). In the case of vanillic acid, the guaiacol concentrations were significantly different among cells activated from spores produced on PDA and BATA (P < 0.05). The obtained results suggested that type of sporulation medium composition may affect the guaiacol concentration depending on inoculum level and type of precursor present in the apple juice

Effects of pomegranate and pomegranate-apple blend juices on the growth characteristics of Alicyclobacillus acidoterrestris DSM 3922 type strain vegetative cells and spores

The present study examined the growth characteristics of Alicyclobacillus acidoterrestris DSM 3922 vegetative cells and spores after inoculation into apple, pomegranate and pomegranate-apple blend juices (10, 20, 40 and 80%, v/v). Also, the effect of sporulation medium was tested using mineral [Bacillus acidoterrestris agar (BATA) and Bacillus acidocaldarius agar (BAA)] and non-mineral containing media [potato dextrose agar (PDA) and malt extract agar (MEA)]. The juice samples were inoculated separately with approximately 105CFU/mL cells or spores from different sporulation media and then incubated at 37°C for 336h. The number of cells decreased significantly with increasing pomegranate juice concentration in the blend juices and storage time (p<0.001). Based on the results, 3.17, 3.53, and 3.72 log cell reductions were observed in 40%, 80% blend and pomegranate juices, respectively while the cell counts attained approximately 7.17logCFU/mL in apple juice after 336h. On the other hand, the cell growth was inhibited for a certain time, and then the numbers started to increase after 72 and 144h in 10% and 20% blend juices, respectively. After 336h, total population among spores produced on PDA, BATA, BAA and MEA indicated 1.49, 1.65, 1.67, and 1.28 log reductions in pomegranate juice; and 1.51, 1.38, 1.40 and 1.16 log reductions in 80% blend juice, respectively. The inhibitory effects of 10%, 20% and 40% blend juices varied depending on the sporulation media used. The results obtained in this study suggested that pomegranate and pomegranate-apple blend juices could inhibit the growth of A. acidoterrestris DSM 3922 vegetative cells and spores

Extracellular enzyme production and enterotoxigenic gene profiles of Bacillus cereus and Bacillus thuringiensis strains isolated from cheese in Turkey

The aim of the present study was to investigate the biochemical characteristics, extracellular enzyme production and enterotoxigenic genes contents of 6 Bacillus cereus and 22 Bacillus thuringiensis strains, isolated from 100 cheese samples in Turkey. Crystal morphologies of B. thuringiensis strains were found either spherical (n = 12) or spherical and irregular-shaped (n = 10) by phase contrast microscopy. B. cereus and B. thuringiensis strains were found to produce extracellular enzymes, respectively: gelatinase (83% and 91%), DNase (83% and 77%), lecithinase (83% and 95%), protease on skim milk agar (100% and 100%), protease on milk agar (100% and 91%), protease on casein agar (83% and 77%), xylanase (100% and 45%), and cellulase (0% and 41%), and amylase (83% and 27%). All of the strains, except for Bt-D1, hydrolyzed Tween 20 (96%), but not Tween 80 or tributyrin. Pectinolytic activity was obtained to be the least frequent (4%). PCR analysis showed that all strains contained nheA, nheB, nheC and hblD genes. The hblA and hblC genes were present in 2 and 4 of B. thuringiensis strains, respectively. The bceT gene was detected in 1 B. cereus and 9 B. thuringiensis strains. The entFM gene was detected more frequently in B. thuringiensis (82%) than in B. cereus strains (50%). To our knowledge, this is the first report about the isolation and identification of enterotoxigenic B. cereus and B. thuringiensis strains from cheese samples in Turkey