155 research outputs found
Gamificación en la enseñanza de la ortografía en los estudiantes del sexto año de educación básica
In this research work we focus on the application of the use of video games as a motivating and playful educational tool during the learning process. The objective of this study is to determine the incidence of gamification in the teaching of spelling in students in the sixth year of basic education at the Carolina de Febres Cordero school, with a population of 21 participants. The work is of a quasi-experimental type, with a longitudinal cohort approach, with a quantitative measurement, the technique to be used will be the written test applied in two times in a pretest and a post. Genially is the platform used to develop interactive presentations and games with different animations, enriching content and practice, resulting in significant changes in terms of linguistic training, improving your writing in a pleasant and lasting way?En el presente trabajo de investigación nos centramos en la aplicación del uso de video juegos como herramienta educativa motivadora y lúdica durante el proceso de aprendizaje. El objetivo en este estudio es determinar la incidencia de la gamificación en la enseñanza de la ortografía en los estudiantes del sexto año de educación básica de la escuela Carolina de Febres Cordero, contando con una población de 21 participantes. El trabajo es de tipo cuasi experimental, con un enfoque de cohorte longitudinal, con una medición cuantitativa, la técnica a utilizarse va a ser la prueba escrita aplicada en dos tiempos en un pretest y post. Genially es la plataforma que se utiliza para desarrollar presentaciones y juegos interactivos con diferentes animaciones enriqueciendo el contenido y la práctica, teniendo resultados cambios significativos en cuanto a la formación de la lingüística, mejorando su escritura de forma placentera y duradera
Podcast como herramienta de enseñanza en la expresión oral en estudiantes de octavo de básica
The use of podcasts in Latin America and mainly in Ecuador is not encouraging in terms of audience numbers, so it is necessary to educate the community so that they can listen to and produce podcasts. The sample for the study consisted of 34 students in the eighth year of primary school at the Unidad Educativa Remigio Crespo Toral. The experimental design of the study was qualitative and quantitative by means of a survey data collection technique and instrument. The data obtained in the study were processed using the SPSS version 25 statistical package for the Windows operating system. The statistical analyses applied to the samples are descriptive and frequency statistics. It is determined that there is no difference in general, all of them consider above 75% in most of the answers the importance of this educational tool on the classes, being women the most motivated in terms of this educational mechanism, one or one of the female students. Since they were anonymous surveys, they did not consider its use to be useful, and it would be convenient to investigate future mechanisms so that it can be used to attract the students' attention.El uso del podcast en Latinoamérica y principalmente en Ecuador no son números alentadores en la audiencia, por eso es necesario educar a la comunidad para que puedan escuchar y producir podcast En la actualidad la sociedad exige un nivel de comunicación oral muy alto como de redacción escrita. La muestra para el estudio está compuesta por 34 estudiantes del octavo año de básica de la Unidad Educativa Remigio Crespo Toral. El diseño experimental del estudio fue de tipo cualitativo y cuantitativo por medio de una técnica e instrumento de toma de datos de la encuesta. Los datos obtenidos en el estudio se han procesado mediante el paquete estadístico SPSS versión 25 para el sistema operativo de Windows. Los análisis estadísticos aplicados sobre las muestran son los estadísticos descriptivos y frecuencia. Se determinan que no existen diferencia en cuanto al general todos consideran por arriba del 75% en la mayoría de las respuestas la importancia de esta herramienta educación sobre las clases siendo las mujeres las más motivadas en cuanto a este mecanismo de educación, de las respuestas una o una de las estudiantes. Debido a que eran encuestas anónimas no considera útil su utilización siendo conveniente indagar a futuro mecanismo para que englobe más la atención de los estudiantes
New findings in the signaling pathways of cis and trans platinum Iodido complexes' interaction with DNA of cancer cells
We have selected a series of aliphatic amine platinum compounds bearing chloride and/or iodide as the leaving groups. The complexes' cytotoxicity and interaction with DNA indicated differences in the reactivity. Now, we are reporting on the analysis of their molecular mechanism of action on gastric cancer cells. Our data reveals differences between them. Chlorido drugs showed similar behavior to cisplatin; they both required p53 to induce apoptosis but only cis-ipa showed DNA damage requirement for apoptosis induction. On the contrary, cis and trans iodido induced cell death independent of p53 activity, and they induced cell death through Bid activation, so their toxicity could be enhanced in a combined treatment with novel Bcl-2 protein family inhibitors. We also report the structural features of the DNA adduct for one of the complexes by X-ray diffraction. These findings represent a step forward in the search for new platinum-derived agents more specific and effective in the treatment of cancerAuthors A.G.Q., M.C., and A.A.-V. received funding from MINECO CTQ2015-68779-R. I.S.P. received funding from P17-01401 (supported by FEDER funds) from Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, SpainNPL is a fellow of the Programa de Doctorado Biociencias Moleculares UAM, Madrid, Spain. NPL was supported by a fellowship Programa de Formación de Profesorado Universitario REF: FPU15/0466
Functional analysis of the Phycomyces carRA gene encoding the enzymes phytoene synthase and lycopene cyclase.
[EN] Phycomyces carRA gene encodes a protein with two domains. Domain R is characterized by red carR mutants that accumulate lycopene. Domain A is characterized by white carA mutants that do not accumulate significant amounts of carotenoids. The carRA-encoded protein was identified as the lycopene cyclase and phytoene synthase enzyme by sequence homology with other proteins. However, no direct data showing the function of this protein have been reported so far. Different Mucor circinelloides mutants altered at the phytoene synthase, the lycopene cyclase or both activities were transformed with the Phycomyces carRA gene. Fully transcribed carRA mRNA molecules were detected by Northern assays in the transformants and the correct processing of the carRA messenger was verified by RT-PCR. These results showed that Phycomyces carRA gene was correctly expressed in Mucor. Carotenoids analysis in these transformants showed the presence of ß-carotene, absent in the untransformed strains, providing functional evidence that the Phycomyces carRA gene complements the M. circinelloides mutations. Co-transformation of the carRA cDNA in E. coli with different combinations of the carotenoid structural genes from Erwinia uredovora was also performed. Newly formed carotenoids were accumulated showing that the Phycomyces CarRA protein does contain lycopene cyclase and phytoene synthase activities. The heterologous expression of the carRA gene and the functional complementation of the mentioned activities are not very efficient in E. coli. However, the simultaneous presence of both carRA and carB gene products from Phycomyces increases the efficiency of these enzymes, presumably due to an interaction mechanism
A comparison between petrous bone and tooth, femur and tibia DNA analysis from degraded skeletal remains
Skeletal remains are the only biological material that remains after long periods;
however, environmental conditions such as temperature, humidity, andpHaffect
DNA preservation, turning skeletal remains into a challenging sample for DNA
laboratories. Sample selection is a key factor, and femur and tooth have been
traditionally recommended as the best substrate of genetic material. Recently,
petrous bone (cochlear area) has been suggested as a better option due to its
DNA yield. This research aims to evaluate the efficiency of petrous bone compared
to other cranium samples (tooth) and postcranial long bones (femur and
tibia). A total amount of 88 samples were selected from 38 different individuals.
The samples were extracted by using an organic extraction protocol, DNA quantification
by Quantifiler Trio kit and amplified with GlobalFiler kit. Results show
that petrous bone outperforms other bone remains in quantification data, yielding
15–30 times more DNA than the others. DNA profile data presented likeness
between petrous bone and tooth regarding detected alleles; however, the amount
of DNA extracted in petrous bones allowed us to obtain more informative DNA
profiles with superior quality. In conclusion, petrous bone or teeth sampling is
recommended if DNA typing is going to be performed with environmentally
degraded skeletal remains
Usefulness of Microbiome for Forensic Geolocation: A Review
Forensic microbiomics is a promising tool for crime investigation. Geolocation, which
connects an individual to a certain place or location by microbiota, has been fairly well studied in
the literature, and several applications have been found. The aim of this review is to highlight the
main findings in this field, including the current sample storage, DNA extraction, sequencing and
data analysis techniques that are being used, and its potential applications in human trafficking
and ancient DNA studies. Second, the challenges and limitations of forensic microbiomics and
geolocation are emphasised, providing recommendations for the establishment of this tool in the
forensic science community
GITAD 2020: quality assurance test through 20 years of experience
Supplementary Information The online version contains supplementary
material available at https://doi. org/10.1007/s00414-022-02802-4.GITAD (Grupo Iberoamericano de Trabajo en Análisis de DNA) was founded in 1998 as the first operational group of
AICEF (Academia Iberoamericana de Criminalística y Estudios Forenses), formally created in 1999. The mission and
the vision of GITAD are to promote the development of forensic genetics in Ibero-American countries and to achieve the
maximum level of innovation and quality in each country, and with that aim, a proficiency test was developed. Since 1999,
the member laboratories receive four reference samples with the objective of obtaining the genetic profile with their routine
protocols, a theoretical exercise since 2003, and since 2007, it was incorporated a forensic sample, which changes every
year. The consensus results and the different discrepancies are discussed in an annual meeting. This article illustrates the
evolution of the proficiency test through 20 years from different points of view: the increase of participant laboratories, the
evolution of the different DNA typing techniques reported by the Ibero-American participant laboratories, the challenges
that the proficiency test have met, and future perspectives for a continuous improvement of the proficiency test, especially
regarding its accreditation under ISO 17043Universidad de Granada/CBU
Creación de recursos multimedia de autoaprendizaje para la docencia práctica de Genética. Valoración de su eficacia en la adquisición de habilidades y competencias
Memoria ID-0225. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2016-2017
A comparison of five DNA extraction methods from degraded human skeletal remains
Extracting DNA from degraded human remains poses a challenge for any forensic genetics laboratory, as it
requires efficient high-throughput methods. While little research has compared different techniques, silica
in suspension has been identified in the literature as the best method for recovering small fragments, which
are often present in these types of samples. In this study, we tested five DNA extraction protocols on 25
different degraded skeletal remains. Including the humerus, ulna, tibia, femur, and petrous bone. The five
protocols were organic extraction by phenol/chloroform/isoamyl alcohol, silica in suspension, High Pure
Nucleic Acid Large Volume silica columns (Roche), InnoXtract™ Bone (InnoGenomics), and PrepFiler™ BTA
with AutoMate™ Express robot (ThermoFisher). We analysed five DNA quantification parameters (small
human target quantity, large human target quantity, human male target quantity, degradation index, and
internal PCR control threshold), and five DNA profile parameters (number of alleles with peak height higher
than analytic and stochastic threshold, average relative fluorescence units (RFU), heterozygous balance, and
number of reportable loci) were analysed. Our results suggest that organic extraction by phenol/chloroform/
isoamyl alcohol was the best performing method in terms of both quantification and DNA profile
results. However, Roche silica columns were found to be the most efficient method
Empleo de la plataforma multimedia Kahoot! para fomentar el aprendizaje interactivo en las clases de teoría y seminarios de Genética
Memoria ID-0093. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2017-2018
- …