158 research outputs found
ANALYSIS OF NEURAL ACTIVITY OF THE HUMAN BASAL GANGLIA IN DYSTONIA: A REVIEW
Deep brain stimulation of the globus pallidus internus is an efective symptomatic treatment for pharmacoresistant dystonic syndromes, where pathophysiological mechanisms of action are not yet fully understood. The aim of this review article is to provide an overview of the state-of-the-art approaches for processing of microelectrode recordings in dystonia; in order to define biomarkers to identify patients who will benefit from the clinical deep brain stimulation. For this purpose, the essential elements of microelectrode processing are examined. Next, we investigate a real example of spike sorting processing in this field. Herein, we describe baseline elements of microrecordings processing including data collection, preprocessing phase, features computation, spike detection and sorting and finally, advanced spike train data analysis. This study will help readers acquire the necessary information about these elements and their associated techniques. Thus, this study is supposed to assist during identification and proposal of interesting clinical hypotheses in the field of single unit neuronal recordings in dystonia
Spike detection and sorting: combining algebraic differentiations with ICA
International audienceA new method for action potentials detection is proposed. The method is based on a numerical differentiation, as recently intro- duced from operational calculus. We show that it has good performance as compared to existing methods. We also combine the proposed method with ICA in order to obtain spike sorting
Extracellular electrophysiology with close-packed recording sites: spike sorting and characterization
Advances in recording technologies now allow us to record populations of neurons simultaneously, data necessary to understand the network dynamics of the brain. Extracellular probes are fabricated with ever greater numbers of recording sites to capture the activity of increasing numbers of neurons. However, the utility of this extracellular data is limited by an initial analysis step, spike sorting, that extracts the activity patterns of individual neurons from the extracellular traces. Commonly used spike sorting methods require manual processing that limits their scalability, and errors can bias downstream analyses. Leveraging the replication of the activity from a single neuron on nearby recording sites, we designed a spike sorting method consisting of three primary steps: (1) a blind source separation algorithm to estimate the underlying source components, (2) a spike detection algorithm to find the set of spikes from each component best separated from background activity and (3) a classifier to evaluate if a set of spikes came from one individual neuron. To assess the accuracy of our method, we simulated multi-electrode array data that encompass many of the realistic variations and the sources of noise in in vivo neural data. Our method was able to extract individual simulated neurons in an automated fashion without any errors in spike assignment. Further, the number of neurons extracted increased as we increased recording site count and density. To evaluate our method in vivo, we performed both extracellular recording with our close-packed probes and a co-localized patch clamp recording, directly measuring one neuron’s ground truth set of spikes. Using this in vivo data we found that when our spike sorting method extracted the patched neuron, the spike assignment error rates were at the low end of reported error rates, and that our errors were frequently the result of failed spike detection during bursts where spike amplitude decreased into the noise. We used our in vivo data to characterize the extracellular recordings of burst activity and more generally what an extracellular electrode records. With this knowledge, we updated our spike detector to capture more burst spikes and improved our classifier based on our characterizations
Unsupervised neural spike identification for large-scale, high-density micro-electrode arrays
This work deals with the development and evaluation of algorithms that extract sequences of single neuron action potentials from extracellular recordings of superimposed neural activity - a task commonly referred to as spike sorting. Large ( electrodes) and dense (subcellular spatial sampling) CMOS-based micro-electrode-arrays allow to record from hundreds of neurons simultaneously. State of the art algorithms for up to a few hundred sensors are not directly applicable to this type of data. Promising modern spike sorting algorithms that seek the statistically optimal solution or focus on real-time capabilities need to be initialized with a preceding sorting. Therefore, this work focused on unsupervised solutions, in order to learn the number of neurons and their spike trains with proper resolution of both temporally and spatiotemporally overlapping activity from the extracellular data alone.
Chapter (1) informs about the nature of the data, a model based view and how this relates to spike sorting in order to understand the design decisions of this thesis. The main materials and methods chapter (2) bundles the infrastructural work that is independent of but mandatory for the development and evaluation of any spike sorting method.
The main problem was split in two parts. Chapter (3) assesses the problem of analyzing data from thousands of densely integrated channels in a divide-and-conquer fashion.
Making use of the spatial information of dense 2D arrays, regions of interest (ROIs) with boundaries adapted to the electrical image of single or multiple neurons were automatically constructed. All ROIs could then be processed in parallel. Within each region of interest the maximum number of neurons could be estimated from the local data matrix alone. An independent component analysis (ICA) based sorting was used to identify units within ROIs. This stage can be replaced by another suitable spike sorting algorithm to solve the local problem. Redundantly identified units across different ROIs were automatically fused into a global solution. The framework was evaluated on both real as well as simulated recordings with ground truth. For the latter it was shown that a major fraction of units could be extracted without any error. The high-dimensional data can be visualized after automatic sorting for convenient verification. Means of rapidly separating well from poorly isolated neurons were proposed and evaluated.
Chapter (4) presents a more sophisticated algorithm that was developed to solve the local problem of densely arranged sensors. ICA assumes the data to be instantaneously mixed, thereby reducing spatial redundancy only and ignoring the temporal structure of extracellular data. The widely accepted generative model describes the intracellular spike trains to be convolved with their extracellular spatiotemporal kernels. To account for the latter it was assessed thoroughly whether convolutive ICA (cICA) could increase sorting performance over instantaneous ICA. The high computational complexity of cICA was dealt with by automatically identifying relevant subspaces that can be unmixed in parallel. Although convolutive ICA is suggested by the data model, the sorting results were dominated by the post-processing for realistic scenarios and did not outperform ICA based sorting. Potential alternatives are discussed thoroughly and bounded from above by a supervised sorting.
This work provides a completely unsupervised spike sorting solution that enables the extraction of a major fraction of neurons with high accuracy and thereby helps to overcome current limitations of analyzing the high-dimensional datasets obtained from simultaneously imaging the extracellular activity from hundreds of neurons with thousands of electrodes
Untersuchung von Verarbeitungsalgorithmen zur automatischen Auswertung neuronaler Signale aus Multielektroden-Arrays
Mit Hilfe von Multielektroden-Arrays (MEAs) können viele Zellen gleichzeitig kontaktiert und deren elektrische Aktivität abgeleitet werden. Für die weitere Analyse müssen die abgeleiteten Signale in ihre Einzelbestandteile zerlegt werden. Dieser Vorgang wird als Spike Sorting bezeichnet. In der vorliegenden Arbeit werden Ansätze für ein vollständig automatisiertes Spike Sorting vorgestellt und untersucht. Dabei werden Verfahren aufgezeigt, die mit Hilfe von adaptiven Verfahren die abgeleiteten Zellsignale optimal filtern und automatisch in deren Einzelkomponenten zerlegen
Investigating information processing within the brain using multi-electrode array (MEA) electrophysiology data
How a stimulus, such as an odour, is represented in the brain is one of the main
questions in neuroscience. It is becoming clearer that information is encoded by
a population of neurons, but, how the spiking activity of a population of neurons
conveys this information is unknown. Several population coding hypotheses have
formulated over the years, and therefore, to obtain a more definitive answer as to
how a population of neurons represents stimulus information we need to test, i.e.
support or falsify, each of the hypotheses. One way of addressing these hypotheses
is to record and analyse the activity of multiple individual neurons from the brain
of a test subject when a stimulus is, and is not, presented. With the advent of multi
electrode arrays (MEA) we can now record such activity. However, before we can
investigate/test the population coding hypotheses using such recordings, we need to
determine the number of neurons recorded by the MEA and their spiking activity,
after spike detection, using an automatic spike sorting algorithm (we refer to the
spiking activity of the neurons extracted from the MEA recordings as MEA sorted
data). While there are many automatic spike sorting methods available, they have
limitations. In addition, we are lacking methods to test/investigate the population
coding hypotheses in detail using the MEA sorted data. That is, methods that
show whether neurons respond in a hypothesised way and, if they do, shows how
the stimulus is represented within the recorded area. Thus, in this thesis, we were
motivated to, firstly, develop a new automatic spike sorting method, which avoids
the limitations of other methods. We validated our method using simulated and
biological data. In addition, we found our method can perform better than other
standard methods. We next focused on the population rate coding hypothesis (i.e.
the hypothesis that information is conveyed in the number of spikes fired by a pop-
ulation of neurons within a relevant time period). More specifically, we developed
a method for testing/investigating the population rate coding hypothesis using the
MEA sorted data. That is, a method that uses the multi variate analysis of variance
(MANOVA) test, where we modified its output, to show the most responsive subar-
eas within the recorded area. We validated this using simulated and biological data.
Finally, we investigated whether noise correlation between neurons (i.e. correlations
in the trial to trial variability of the response of neurons to the same stimulus) in
a rat's olfactory bulb can affect the amount of information a population rate code
conveys about a set of stimuli. We found that noise correlation between neurons
was predominately positive, which, ultimately, reduced the amount of information
a population containing >45 neurons could convey about the stimuli by ~30%
Recommended from our members
Dysfunction of neurovascular/metabolic coupling in chronic focal epilepsy
In this study, we aim to evaluate the mechanisms underlying the neuro-vascular/metabolic coupling in the epileptogenic cortices of rats with chronic focal epilepsy. To that end, we first analyzed intracranial recordings (electrophysiology, laser Doppler flowmetry and optical imaging) obtained from the seizure onset zones during ictal periods and then used these data to fit a metabolically-coupled balloon model. This biophysical model is an extension of the standard balloon model with modulatory effects of changes in tissue oxygenation, capillary dynamics and variable O2 extraction fraction. As previously reported using acute seizure models, we found that there is a significant higher contribution from high local field potential frequency bands to the cerebral blood flow (CBF) responses in the epileptogenic cortices during ictal neuronal activities. The hemodynamic responses associated with ictal activities were distance-dependent with regard to the seizure focus, though varied in profiles from those obtained from acute seizure models. Parameters linking the CBF and relative concentration of deoxy-hemoglobin to neuronal activity in the biophysical model were significantly different between epileptic and normal rats. In particular, we found that the coefficient associated with the strength of the functional hyperemic response was significantly larger in the epileptogenic cortices, although changes in hemoglobin concentration associated with ictal activity reflected the existence of a significantly higher baseline for oxygen metabolism in the epileptogenic cortices
Advances in Neural Signal Processing
Neural signal processing is a specialized area of signal processing aimed at extracting information or decoding intent from neural signals recorded from the central or peripheral nervous system. This has significant applications in the areas of neuroscience and neural engineering. These applications are famously known in the area of brain–machine interfaces. This book presents recent advances in this flourishing field of neural signal processing with demonstrative applications
Advances in Neural Signal Processing
Neural signal processing is a specialized area of signal processing aimed at extracting information or decoding intent from neural signals recorded from the central or peripheral nervous system. This has significant applications in the areas of neuroscience and neural engineering. These applications are famously known in the area of brain–machine interfaces. This book presents recent advances in this flourishing field of neural signal processing with demonstrative applications
- …