Combined super-/substring and super-/subsequence problems

Super-/substring problems and super-/subsequence problems are well-known problems in stringology that have applications in a variety of areas, such as manufacturing systems design and molecular biology. Here we investigate the complexity of a new type of such problem that forms a combination of a super-/substring and a super-/subsequence problem. Moreover we introduce different types of minimal superstring and maximal substring problems. In particular, we consider the following problems: given a set L of strings and a string S, (i) find a minimal superstring (or maximal substring) of L that is also a supersequence (or a subsequence) of S, (ii) find a minimal supersequence (or maximal subsequence) of L that is also a superstring (or a substring) of S. In addition some non-super-/non-substring and non-super-/non-subsequence variants are studied. We obtain several NP-hardness or even MAX SNP-hardness results and also identify types of "weak minimal" superstrings and "weak maximal" substrings for which (i) is polynomial-time solvable

Combined super-/substring and super-/subsequence problems

Super-/substring problems and super-/subsequence problems are well-known problems in stringology that have applications in a variety of areas, such as manufacturing systems design and molecular biology. Here we investigate the complexity of a new type of such problem that forms a combination of a super-/substring and a super-/subsequence problem. Moreover we introduce different types of minimal superstring and maximal substring problems. In particular, we consider the following problems: given a set L of strings and a string S, (i) find a minimal superstring (or maximal substring) of L that is also a supersequence (or a subsequence) of S, (ii) find a minimal supersequence (or maximal subsequence) of L that is also a superstring (or a substring) of S. In addition some non-super-/non-substring and non-super-/non-subsequence variants are studied. We obtain several NP-hardness or even MAX SNP-hardness results and also identify types of “weak minimal” superstrings and “weak maximal” substrings for which (i) is polynomial-time solvable

Expected length of longest common subsequences

A longest common subsequence of two sequences is a sequence that is a subsequence of both the given sequences and has largest possible length. It is known that the expected length of a longest common subsequence is proportional to the length of the given sequences. The proportion, denoted by 7k, is dependent on the alphabet size k and the exact value of this proportion is not known even for a binary alphabet. To obtain lower bounds for the constants 7k, finite state machines computing a common subsequence of the inputs are built. Analysing the behaviour of the machines for random inputs we get lower bounds for the constants 7k. The analysis of the machines is based on the theory of Markov chains. An algorithm for automated production of lower bounds is described. To obtain upper bounds for the constants 7k, collations pairs of sequences with a marked common subsequence - are defined. Upper bounds for the number of collations of ‘small size’ can be easily transformed to upper bounds for the constants 7k. Combinatorial analysis is used to bound the number of collations. The methods used for producing bounds on the expected length of a common subsequence of two sequences are also used for other problems, namely a longest common subsequence of several sequences, a shortest common supersequence and a maximal adaptability

CAD Tools for DNA Micro-Array Design, Manufacture and Application

Motivation: As the human genome project progresses and some microbial and eukaryotic genomes are recognized, numerous biotechnological processes have attracted increasing number of biologists, bioengineers and computer scientists recently. Biotechnological processes profoundly involve production and analysis of highthroughput experimental data. Numerous sequence libraries of DNA and protein structures of a large number of micro-organisms and a variety of other databases related to biology and chemistry are available. For example, microarray technology, a novel biotechnology, promises to monitor the whole genome at once, so that researchers can study the whole genome on the global level and have a better picture of the expressions among millions of genes simultaneously. Today, it is widely used in many fields- disease diagnosis, gene classification, gene regulatory network, and drug discovery. For example, designing organism specific microarray and analysis of experimental data require combining heterogeneous computational tools that usually differ in the data format; such as, GeneMark for ORF extraction, Promide for DNA probe selection, Chip for probe placement on microarray chip, BLAST to compare sequences, MEGA for phylogenetic analysis, and ClustalX for multiple alignments. Solution: Surprisingly enough, despite huge research efforts invested in DNA array applications, very few works are devoted to computer-aided optimization of DNA array design and manufacturing. Current design practices are dominated by ad-hoc heuristics incorporated in proprietary tools with unknown suboptimality. This will soon become a bottleneck for the new generation of high-density arrays, such as the ones currently being designed at Perlegen [109]. The goal of the already accomplished research was to develop highly scalable tools, with predictable runtime and quality, for cost-effective, computer-aided design and manufacturing of DNA probe arrays. We illustrate the utility of our approach by taking a concrete example of combining the design tools of microarray technology for Harpes B virus DNA data

Subsequences and Supersequences of Strings

Stringology - the study of strings - is a branch of algorithmics which been the sub-ject of mounting interest in recent years. Very recently, two books [M. Crochemore and W. Rytter, Text Algorithms, Oxford University Press, 1995] and [G. Stephen, String Searching Algorithms, World Scientific, 1994] have been published on the subject and at least two others are known to be in preparation. Problems on strings arise in information retrieval, version control, automatic spelling correction, and many other domains. However the greatest motivation for recent work in stringology has come from the field of molecular biology. String problems occur, for example, in genetic sequence construction, genetic sequence comparison, and phylogenetic tree construction. In this thesis we study a variety of string problems from a theoretical perspective. In particular, we focus on problems involving subsequences and supersequences of strings