Effect of mercury vapor inhalation on rat ovary: stereology and histopathology

AimMercury, an environmental contaminant, is a risk factor for health in whole living organisms. In this study, we investigated whether mercury vapor (HgO) inhalation has an effect on rat ovary. MethodsTwelve Wistar albino rats were divided equally into experimental (Hg) and control groups (n = 6). Animals in the Hg group were exposed to HgO for 45 days at a dose 1 mg/m(3)/day, after which, histological and stereological assessment were carried out. ResultsOvaries exposed to HgO had histo-morphometric alterations. HgO inhalation resulted in reduction of the total number of primordial, primary and Graaf follicles. Also, mean volume of ovary, medulla and cortex, corpus luteum (c.luteum) and Graaf follicles was decreased in the Hg group. Moreover, there was a significant increase in total volume of the atretic follicles. On light microscopy, thickening of tunica albuginea, increase of fibrils within the connective tissue, congestion of the capillaries and venous vessels, thinned walls and fibrin deposition in some large blood vessels, and edema were seen. Also, irregular follicle and oocyte borders, and hydropic degeneration in follicular granulosa cells were detected. ConclusionStructural alterations could be attributed to the toxic influence of HgO on rat ovary. The use of Hg should therefore be more controlled to minimize its toxic effect

A short primer on lung stereology

The intention of this short primer is to raise your appetite for proper quantitative assessment of lung micro-structure. The method of choice for obtaining such data is stereology. Rooted in stochastic geometry, stereology provides simple and efficient tools to obtain quantitative three-dimensional information based on measurements on nearly two-dimensional microscopic sections. In this primer, the basic concepts of stereology and its application to the lung are introduced step by step along the workflow of a stereological study. The integration of stereology in your laboratory work will help to improve its quality. In a broader context, stereology may also be seen as a contribution to good scientific practice

A stereological study of the effects of mercury inhalation on the cerebellum

Mercury in the environment that arises from organic and inorganic sources can cause irreversible damage to the nervous system. Toxicity may be direct or may arise from interactions with other metals in the environment. We evaluated the possible effects of mercury vapor on rat cerebellum. Twelve adult female rats were divided into control and experimental groups. The rats in the experimental group were exposed to mercury vapor for 9 h/day for 45 days. Cerebellar tissue samples were evaluated using stereology and for histopathology. The total number of Purkinje cells was estimated using a physical disector method. We found that in the experimental group, overall volume decreased and the number of Purkinje cells was reduced. We also found cellular damage including pycnotic nuclei, eosinophilic cytoplasm and vacuolization; these features were absent in the control group. We found that chronic exposure to inorganic mercury vapor is toxic to the cerebellum

A Feasibility Study of Quantifying Longitudinal Brain Changes in Herpes Simplex Virus (HSV) Encephalitis Using Magnetic Resonance Imaging (MRI) and Stereology.

OBJECTIVES: To assess whether it is feasible to quantify acute change in temporal lobe volume and total oedema volumes in herpes simplex virus (HSV) encephalitis as a preliminary to a trial of corticosteroid therapy. METHODS: The study analysed serially acquired magnetic resonance images (MRI), of patients with acute HSV encephalitis who had neuroimaging repeated within four weeks of the first scan. We performed volumetric measurements of the left and right temporal lobes and of cerebral oedema visible on T2 weighted Fluid Attenuated Inversion Recovery (FLAIR) images using stereology in conjunction with point counting. RESULTS: Temporal lobe volumes increased on average by 1.6% (standard deviation (SD 11%) in five patients who had not received corticosteroid therapy and decreased in two patients who had received corticosteroids by 8.5%. FLAIR hyperintensity volumes increased by 9% in patients not receiving treatment with corticosteroids and decreased by 29% in the two patients that had received corticosteroids. CONCLUSIONS: This study has shown it is feasible to quantify acute change in temporal lobe and total oedema volumes in HSV encephalitis and suggests a potential resolution of swelling in response to corticosteroid therapy. These techniques could be used as part of a randomized control trial to investigate the efficacy of corticosteroids for treating HSV encephalitis in conjunction with assessing clinical outcomes and could be of potential value in helping to predict the clinical outcomes of patients with HSV encephalitis

Analysis of Vesicular Basalts and Lava Emplacement Processes for Application as a Paleobarometer/Paleoaltimeter

We have developed a method for determining paleoelevations of highland areas on the basis of the vesicularity of lava flows. Vesicular lavas preserve a record of paleopressure at the time and place of emplacement because the difference in internal pressure in bubbles at the base and top of a lava flow depends on atmospheric pressure and lava flow thickness. At the top of the flow, the pressure is simply atmospheric pressure, while at the base, there is an additional contribution of hydrostatic lava overburden. Thus the modal size of the vesicle (bubble) population is larger at the top than at the bottom. This leads directly to paleoatmospheric pressure because the thickness of the flow can easily be measured in the field, and the vesicle sizes can now be accurately measured in the lab. Because our recently developed technique measures paleoatmospheric pressure, it is not subject to uncertainties stemming from the use of climate‐sensitive proxies, although like all measurements, it has its own sources of potential error. Because measurement of flow thickness presupposes no inflation or deflation of the flow after the size distribution at the top and bottom is “frozen in,” it is essential to identify preserved flows in the field that show clear signs of simple emplacement and solidification. This can be determined by the bulk vesicularity and size distribution as a function of stratigraphic position within the flow. By examining the stratigraphic variability of vesicularity, we can thus reconstruct emplacement processes. It is critical to be able to accurately measure the size distribution in collected samples from the tops and bottoms of flows because our method is based on the modal size of the vesicle population. Previous studies have used laborious and inefficient methods that did not allow for practical analysis of a large number of samples. Our recently developed analytical techniques involving high‐resolution x‐ray computed tomography (HRXCT) allow us to analyze the large number of samples required for reliable interpretations. Based on our ability to measure vesicle size to within 1.7% (by volume), a factor analysis of the sensitivity of the technique to atmospheric pressure provides an elevation to within about ±400 m. If we assume sea level pressure and lapse rate have not changed significantly in Cenozoic time, then the difference between the paleoelevation “preserved” in the lavas and their present elevation reflects the amount of uplift or subsidence. Lava can be well dated, and therefore a suite of samples of various ages will constrain the timing of epeirogenic activity independent of climate, erosion rates, or any other environmental factors. We have tested our technique on basalts emplaced at known elevations at the base, flanks, and summit of Mauna Loa. The results of the analysis accurately reconstruct actual elevations, demonstrating the applicability of the technique. The tool we have developed can subsequently be applied to problematic areas such as the Colorado and Tibetan Plateaus to determine the history of uplift

Implementation of Unbiased Stereology Method for Organ Volume Estimation using Image Processing

Stereology had been widely used around the globe for estimating the value of the biological structure. It interlinks the connection between the dimensions using a sample that is 2D for a quantification in 3D structure. Volume estimation using stereology method is the most common and basic quantification that shows an example of how stereology works. Stereology method has a drawback when it comes to a lot of samples, so it is common for a researcher to use the computer to help to quantify and to do repetitive work, which is known as computer-assisted stereology quantification. The purpose of this research is to develop software that can assist researchers using stereology method for estimating the volume of a sample object from its 2D digital images with point counting method. The software was built based on the manual procedure of volume estimation using stereology method performed by experienced researchers. The volume estimation using the software were then compared with the results of the manual procedure by the experts on the same samples, i.e., the kidney and trigeminal ganglion of a rat. The experimental results showed that the relative difference of the volume estimations between manual and computer assisted calculations were 0.93% for rat kidney sample and 10.81%. TA1 trigeminal ganglion sample. Based on the results, the software estimation can be used for further analysis