Seizure development in the acute intrahippocampal epileptic focus.

Currently, an epileptic seizure is considered to involve a temporary network that exists for a finite period of time. Formation of this network evolves through spread of epileptiform activity from a seizure onset zone (SOZ). Propagation of seizures evoked by kainic acid injection in hippocampus to different brain areas was analyzed at macro- and micro-intervals. The mean latency of seizure occurrence in different brain areas varied between 0.5 sec and 85 sec (mean 14.9 ± 14.5 (SD)), and it increased after each consecutive seizure in areas located contralateral to the area of injection, but not in the ipsilateral sites. We have shown that only 41% of epileptic individual events in target brain areas were driven by epileptic events generated in the SOZ once the seizure began. Fifty-nine percent of epileptiform events in target areas occurred one millisecond before or after events in the SOZ. These data illustrate that during seizure maintenance, only some individual epileptiform events in areas outside of SOZ could be consistently triggered by the SOZ; and the majority must be triggered by a driver located outside the SOZ or brain areas involved in ictal activity could be coupled to each other via an unknown mechanism such as stochastic resonance

Attention Enhances the Retrieval and Stability of Visuospatial and Olfactory Representations in the Dorsal Hippocampus

Attention enhances the encoding and retrieval of olfactory and visuospatial representations by modulating place field stability, firing rate, and neuronal synchronization of pyramidal cells in the hippocampus

Short-term changes in bilateral hippocampal coherence precede epileptiform events.

International audienceThe mesial temporal lobe epilepsy syndrome (MTLE) is the most common form of focal epilepsies. MTLE patients usually respond very little to pharmacological therapy and surgical resection of temporal brain areas is mandatory. Finding less invasive therapies than resection of the sclerotic hippocampus requires knowledge of the network structures and dynamics involved in seizure generation. Investigation of the time interval immediately preceding seizure onset would help in understanding the initiation mechanisms of the seizure proper and, thereby, possibly improve therapeutical options. Here, we employed the in vivo intrahippocampal kainate model in mice, which is characterized by unilateral histological changes, resembling hippocampal sclerosis observed in human MTLE, and recurrent focal seizures. In these epileptic mice, population spikes occurred during epileptiform events (EEs) in the ipsilateral, histologically changed hippocampus, but also concomitantly in the contralateral, intact hippocampus. We studied synchronization processes between the ipsilateral, sclerotic hippocampus and the contralateral hippocampus immediately preceding the onset of EEs. We show that coherence between the two hippocampi decreased consistently and reliably for all EEs at 8 to 12 s before their onset at high frequencies (>100 Hz), without changes in power in these bands. This early decoupling of the two hippocampi indicates the time range for cellular and network mechanisms leading to increased excitability and/or synchronicity in the tissue and thus ultimately to epileptic seizures

A utilização de sistemas computacionais no desenvolvimento e análise do modelo animal de Kindling Hipocampal/

AnexosOrientador: João Cândido AraújoCo-orientador: Andreas Schulze BonhageDissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Programa de Pós-Graduação em Clínica Cirúrgica. Defesa: Curitiba, 18 de dezembro de 2007.Inclui bibliografiaÁrea de concentração: Informática no Ensino e na Pesquisa em Cirurgi