Experimental validation of a mathematical model for the evolution of the particle morphology of waterborne polymer-polymer hybrids: paving the way to the design and implementation of optimal polymerization strategies

Polymer-polymer composite nanoparticles allow both the improvement of the performance in stablished applications of waterborne polymer dispersions and targeting new applications that are out of reach of currently available products. The performance of these materials is determined by the particle morphology. To open the way to process optimization and on-line control of the particle morphology, the capability of the recently developed model to predict the evolution of the particle morphology during seeded semibatch emulsion polymerization process was evaluated. Structured polymer particles were synthesized by copolymerization of styrene and butyl acrylate (St-BA) on methyl methacrylate and butyl acrylate (MMA–BA) copolymer seeds of different Tgs. The model captured well the effect of process variables on the evolution of the particle morphology, opening the way to the design and implementation of optimal strategies.The financial support of the RECOBA project (funding from European Framework Horizon 2020, No. 636820) is gratefully acknowledged

Dominance of backward stimulated Raman scattering in gas-filled hollow-core photonic crystal fibers

Backward stimulated Raman scattering in gases provides a promising route to compression and amplification of a Stokes seed-pulse by counter-propagating against a pump-pulse, as has been already demonstrated in various platforms, mainly in free-space. However, the dynamics governing this process when seeded by noise has not yet been investigated in a fully controllable collinear environment. Here we report the first unambiguous observation of efficient noise-seeded backward stimulated Raman scattering in a hydrogen-filled hollow-core photonic crystal fiber. At high gas pressures, when the backward Raman gain is comparable with, but lower than, the forward gain, we report quantum conversion efficiencies exceeding 40% to the backward Stokes at 683 nm from a narrowband 532-nm-pump. The efficiency increases to 65% when the backward process is seeded by a small amount of back-reflected forward-generated Stokes light. At high pump powers the backward Stokes signal, emitted in a clean fundamental mode and spectrally pure, is unexpectedly always stronger than its forward-propagating counterpart. We attribute this striking observation to the unique temporal dynamics of the interacting fields, which cause the Raman coherence (which takes the form of a moving fine-period Bragg grating) to grow in strength towards the input end of the fiber. A good understanding of this process, together with the rapid development of novel anti-resonant-guiding hollow-core fibers, may lead to improved designs of efficient gas-based Raman lasers and amplifiers operating at wavelengths from the ultraviolet to the mid-infrared.Comment: 6 pages and 8 figures in the main section. 4 pages and 5 figures in the supplementary sectio

Extracellular Matrix Aggregates from Differentiating Embryoid Bodies as a Scaffold to Support ESC Proliferation and Differentiation

Embryonic stem cells (ESCs) have emerged as potential cell sources for tissue engineering and regeneration owing to its virtually unlimited replicative capacity and the potential to differentiate into a variety of cell types. Current differentiation strategies primarily involve various growth factor/inducer/repressor concoctions with less emphasis on the substrate. Developing biomaterials to promote stem cell proliferation and differentiation could aid in the realization of this goal. Extracellular matrix (ECM) components are important physiological regulators, and can provide cues to direct ESC expansion and differentiation. ECM undergoes constant remodeling with surrounding cells to accommodate specific developmental event. In this study, using ESC derived aggregates called embryoid bodies (EB) as a model, we characterized the biological nature of ECM in EB after exposure to different treatments: spontaneously differentiated and retinoic acid treated (denoted as SPT and RA, respectively). Next, we extracted this treatment-specific ECM by detergent decellularization methods (Triton X-100, DOC and SDS are compared). The resulting EB ECM scaffolds were seeded with undifferentiated ESCs using a novel cell seeding strategy, and the behavior of ESCs was studied. Our results showed that the optimized protocol efficiently removes cells while retaining crucial ECM and biochemical components. Decellularized ECM from SPT EB gave rise to a more favorable microenvironment for promoting ESC attachment, proliferation, and early differentiation, compared to native EB and decellularized ECM from RA EB. These findings suggest that various treatment conditions allow the formulation of unique ESC-ECM derived scaffolds to enhance ESC bioactivities, including proliferation and differentiation for tissue regeneration applications. © 2013 Goh et al

Multiphase modelling of vascular tumour growth in two spatial dimensions

In this paper we present a continuum mathematical model of vascular tumour growth which is based on a multiphase framework in which the tissue is decomposed into four distinct phases and the principles of conservation of mass and momentum are applied to the normal/healthy cells, tumour cells, blood vessels and extracellular material. The inclusion of a diffusible nutrient, supplied by the blood vessels, allows the vasculature to have a nonlocal influence on the other phases. Two-dimensional computational simulations are carried out on unstructured, triangular meshes to allow a natural treatment of irregular geometries, and the tumour boundary is captured as a diffuse interface on this mesh, thereby obviating the need to explicitly track the (potentially highly irregular and ill-defined) tumour boundary. A hybrid finite volume/finite element algorithm is used to discretise the continuum model: the application of a conservative, upwind, finite volume scheme to the hyperbolic mass balance equations and a finite element scheme with a stable element pair to the generalised Stokes equations derived from momentum balance, leads to a robust algorithm which does not use any form of artificial stabilisation. The use of a matrix-free Newton iteration with a finite element scheme for the nutrient reaction-diffusion equations allows full nonlinearity in the source terms of the mathematical model. Numerical simulations reveal that this four-phase model reproduces the characteristic pattern of tumour growth in which a necrotic core forms behind an expanding rim of well-vascularised proliferating tumour cells. The simulations consistently predict linear tumour growth rates. The dependence of both the speed with which the tumour grows and the irregularity of the invading tumour front on the model parameters are investigated

Viscous evolution of point vortex equilibria: The collinear state

When point vortex equilibria of the 2D Euler equations are used as initial conditions for the corre- sponding Navier-Stokes equations (viscous), typically an interesting dynamical process unfolds at short and intermediate time scales, before the long time single peaked, self-similar Oseen vortex state dom- inates. In this paper, we describe the viscous evolution of a collinear three vortex structure that cor- responds to an inviscid point vortex fixed equilibrium. Using a multi-Gaussian 'core-growth' type of model, we show that the system immediately begins to rotate unsteadily, a mechanism we attribute to a 'viscously induced' instability. We then examine in detail the qualitative and quantitative evolution of the system as it evolves toward the long-time asymptotic Lamb-Oseen state, showing the sequence of topological bifurcations that occur both in a fixed reference frame, and in an appropriately chosen rotating reference frame. The evolution of passive particles in this viscously evolving flow is shown and interpreted in relation to these evolving streamline patterns.Comment: 17 pages, 15 figure

From RE-211 to RE-123. How to control the final microstructure of superconducting single-domains

This paper reviews the usual techniques for producing YBCO-type single-domains and the microstructure of the as-obtained samples. The problems of seed dissolution and parasite nucleations are discussed in details. Formation of microstructural defects, such as pores and cracks, are examined. An important part of this review is devoted to the study of the influence of RE-211 particles [RE2BaCuO5 where RE denotes Y, Yb, Nd, Sm, Dy, Gd, Eu or a mixture of them. Generally Nd4Ba2Cu2O10 is preferred to Nd2BaCuO5] on the microstructure and properties of RE-Ba-Cu-O single-domains. Trapping/Pushing theory is described in order to explain the spatial distribution of RE-211 particles in the RE-123 [(RE)Ba2Cu3O7-d] monoliths. Formation of RE-211-free regions is discussed. Different ways to limit the RE-211 coarsening are reviewed. Microstructural defects in the RE-123 matrix caused by the RE-211 particles are presented. It is also shown that RE-211 particles play a significant role on the mechanical properties of single-domain samples. We finish this review by discussing the Infiltration and Growth process as a good technique to control the microstructure.Comment: review paper to be published in Supercond. Sci. Technol.; 19 figures; 137 references; 37 page

A novel mechanism of action of HER2 targeted immunotherapy is explained by inhibition of NRF2 function in ovarian cancer cells

Nuclear erythroid related factor-2 (NRF2) is known to promote cancer therapeutic detoxification and crosstalk with growth promoting pathways. HER2 receptor tyrosine kinase is frequently overexpressed in cancers leading to uncontrolled receptor activation and signaling. A combination of HER2 targeting monoclonal antibodies shows greater anticancer efficacy than the single targeting antibodies, however, its mechanism of action is largely unclear. Here we report novel actions of anti-HER2 drugs, Trastuzumab and Pertuzumab, involving NRF2. HER2 targeting by antibodies inhibited growth in association with persistent generation of reactive oxygen species (ROS), glutathione (GSH) depletion, reduction in NRF2 levels and inhibition of NRF2 function in ovarian cancer cell lines. The combination of antibodies produced more potent effects than single alone; downregulated NRF2 substrates by repressing the Antioxidant Response (AR) pathway with concomitant transcriptional inhibition of NRF2. We showed the antibody combination produced increased methylation at the NRF2 promoter consistent with repression of NRF2 antioxidant function, as HDAC and methylation inhibitors reversed such produced transcriptional effects. These findings demonstrate a novel mechanism and role for NRF2 in mediating the response of cancer cells to the combination of Trastuzumab and Pertuzumab and reinforce the importance of NRF2 in drug resistance and as a key anticancer target