CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
research
Extracellular Matrix Aggregates from Differentiating Embryoid Bodies as a Scaffold to Support ESC Proliferation and Differentiation
Authors
A Page-McCaw
AK Hadjantonakis
+64 more
AV Ngangan
BE Uygun
BG Ciruna
C Showell
CJ Flaim
CM Zwolinski
D Shook
DA Brafman
DT Scadden
E Coucouvanis
EA Ross
G Bain
G Keller
G Nikolova
H Fujiwara
H Lin
HC Ott
HK Kleinman
I Leivo
Ipsita Banerjee
Irina Kerkis
J Cortiella
J Itskovitz-Eldor
JA DeQuach
JC Boucaut
JC Boucaut
JC Wong
L Schuger
LA Flanagan
LE Dickinson
M Dziadek
MP Lutolf
PH Crossley
Phillip Olsen
PM Crapo
R Nair
R Nair
R Nair
RL Carpenedo
RL Carpenedo
RW Grauss
S Abraham
S Abraham
S Gerecht-Nir
S Levenberg
S Shukla
Saik-Kia Goh
SB Lumpkins
SF Badylak
T Hoshiba
T Hoshiba
T Jensen
T Rozario
TC Doetschman
TD Palmer
TP Kraehenbuehl
TW Gilbert
W Ma
WL Rust
WP Daley
X Li
Y Hieda
Y Okada
Y-M Lin
Publication date
18 April 2013
Publisher
'Public Library of Science (PLoS)'
Doi
Cite
View
on
PubMed
Abstract
Embryonic stem cells (ESCs) have emerged as potential cell sources for tissue engineering and regeneration owing to its virtually unlimited replicative capacity and the potential to differentiate into a variety of cell types. Current differentiation strategies primarily involve various growth factor/inducer/repressor concoctions with less emphasis on the substrate. Developing biomaterials to promote stem cell proliferation and differentiation could aid in the realization of this goal. Extracellular matrix (ECM) components are important physiological regulators, and can provide cues to direct ESC expansion and differentiation. ECM undergoes constant remodeling with surrounding cells to accommodate specific developmental event. In this study, using ESC derived aggregates called embryoid bodies (EB) as a model, we characterized the biological nature of ECM in EB after exposure to different treatments: spontaneously differentiated and retinoic acid treated (denoted as SPT and RA, respectively). Next, we extracted this treatment-specific ECM by detergent decellularization methods (Triton X-100, DOC and SDS are compared). The resulting EB ECM scaffolds were seeded with undifferentiated ESCs using a novel cell seeding strategy, and the behavior of ESCs was studied. Our results showed that the optimized protocol efficiently removes cells while retaining crucial ECM and biochemical components. Decellularized ECM from SPT EB gave rise to a more favorable microenvironment for promoting ESC attachment, proliferation, and early differentiation, compared to native EB and decellularized ECM from RA EB. These findings suggest that various treatment conditions allow the formulation of unique ESC-ECM derived scaffolds to enhance ESC bioactivities, including proliferation and differentiation for tissue regeneration applications. © 2013 Goh et al
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
Public Library of Science (PLOS)
See this paper in CORE
Go to the repository landing page
Download from data provider
Last time updated on 05/06/2019
Name not available
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:d-scholarship.pitt.edu:186...
Last time updated on 23/11/2016
Directory of Open Access Journals
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:doaj.org/article:94749d556...
Last time updated on 13/10/2017
FigShare
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:figshare.com:article/68564...
Last time updated on 12/02/2018
Name not available
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:d-scholarship.pitt.edu:186...
Last time updated on 15/12/2016
D-Scholarship@Pitt
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:d-scholarship.pitt.edu:186...
Last time updated on 19/07/2013
Crossref
See this paper in CORE
Go to the repository landing page
Download from data provider
info:doi/10.1371%2Fjournal.pon...
Last time updated on 01/04/2019