The Future of Challenges to the Alaskan Public School Funding Scheme After State v. Ketchikan

In 2013, the Ketchikan Gateway Borough initiated a challenge to the Alaska public education funding scheme by paying its required local contribution (RLC) to its school district under protest. The Borough subsequently filed a lawsuit against the State of Alaska in 2014. This Note discusses the supreme court’s constitutional analysis of the RLC in State v. Ketchikan. Despite extensive discussion of the RLC in the context of the Alaska Constitution’s Dedicated Funds Clause, the court failed to sufficiently analyze the RLC (a critical component of public school funding) in the context of the state’s responsibility for education—a duty rooted in the Public Schools Clause. This Note will argue that, unlike the challenge to the RLC under the Dedicated Funds Clause, a successful challenge to the RLC under the Public Schools Clause is a possibility. To prevent a hasty legislative response, the State should consider alternative funding schemes less reliant on RLCs before a court order demands it do so, particularly given the disparities in local contributions that are not necessarily proportional to borough revenues as well as the increased criticism of the RLC after State v. Ketchikan

Revisiting Frank–Starling: regulatory light chain phosphorylation alters the rate of force redevelopment (ktr) in a length-dependent fashion

Force and power in cardiac muscle have a known dependence on phosphorylation of the myosin-associated regulatory light chain (RLC). We explore the effect of RLC phosphorylation on the ability of cardiac preparations to redevelop force (ktr ) in maximally activating [Ca2+ ]. Activation was achieved by rapidly increasing the temperature (temperature-jump of 0.5-20ºC) of permeabilized trabeculae over a physiological range of sarcomere lengths (1.85-1.94 μm). The trabeculae were subjected to shortening ramps over a range of velocities and the extent of RLC phosphorylation was varied. The latter was achieved using an RLC-exchange technique, which avoids changes in the phosphorylation level of other proteins. The results show that increasing RLC phosphorylation by 50% accelerates ktr by ∼50%, irrespective of the sarcomere length, whereas decreasing phosphorylation by 30% slows ktr by ∼50%, relative to the ktr obtained for in vivo phosphorylation. Clearly, phosphorylation affects the magnitude of ktr following step shortening or ramp shortening. Using a two-state model, we explore the effect of RLC phosphorylation on the kinetics of force development, which proposes that phosphorylation affects the kinetics of both attachment and detachment of cross-bridges. In summary, RLC phosphorylation affects the rate and extent of force redevelopment. These findings were obtained in maximally activated muscle at saturating [Ca2+ ] and are not explained by changes in the Ca2+ -sensitivity of acto-myosin interactions. The length-dependence of the rate of force redevelopment, together with the modulation by the state of RLC phosphorylation, suggests that these effects play a role in the Frank-Starling law of the heart.Published versio

Empirical model for quasi direct current interruption with a convoluted arc

This contribution considers various aspects of a quasi direct current, convoluted arc produced by a magnetic field (B-field) connected in parallel with an RLC circuit that have not been considered in combination. These aspects are the arc current limitation due to the arc convolution, changes in arc resistance due to the B-field and material ablation, and the relative significance of the RLC circuit in producing an artificial current zero. As a result, it has been possible to produce an empirical equation for predicting the current interruption capability in terms of the B-field magnitude and RLC components

A geometric Birkhoffian formalism for nonlinear RLC networks

The aim of this paper is to give a formulation of the dynamics of nonlinear RLC circuits as a geometric Birkhoffian system and to discuss in this context the concepts of regularity, conservativeness, dissipativeness. An RLC circuit, with no assumptions placed on its topology, will be described by a family of Birkhoffian systems, parameterized by a finite number of real constants which correspond to initial values of certain state variables of the circuit. The configuration space and a special Pfaffian form, called Birkhoffian, are obtained from the constitutive relations of the resistors, inductors and capacitors involved and from Kirchhoff's laws. Under certain assumptions on the voltage-current characteristic for resistors, it is shown that a Birkhoffian system associated to an RLC circuit is dissipative. For RLC networks which contain a number of pure capacitor loops or pure resistor loops the Birkhoffian associated is never regular. A procedure to reduce the original configuration space to a lower dimensional one, thereby regularizing the Birkhoffian, it is also presented. In order to illustrate the results, specific examples are discussed in detail.Comment: 30 pages, 2 figure

Performance evaluation of MPEG-4 video streaming over UMTS networks using an integrated tool environment

Universal Mobile Telecommunications System (UMTS) is a third-generation mobile communications system that supports wireless wideband multimedia applications. This paper investigates the video quality attained in streaming MPEG-4 video over UMTS networks using an integrated tool environment, which comprises an MPEG-4 encoder/decoder, a network simulator and video quality evaluation tools. The benefit of such an integrated tool environment is that it allows the evaluation of real video sources compressed using an MPEG-4 encoder. Simulation results show that UMTS Radio Link Control (RLC) outperforms the unacknowledged mode. The latter mode provides timely delivery but no error recovery. The acknowledged mode can deliver excellent perceived video quality for RLC block error rates up to 30% utilizing a playback buffer at the streaming client. Based on the analysis of the performance results, a self-adaptive RLC acknowledged mode protocol is proposed

Structural insights into RNA processing by the human RISC-loading complex.

Targeted gene silencing by RNA interference (RNAi) requires loading of a short guide RNA (small interfering RNA (siRNA) or microRNA (miRNA)) onto an Argonaute protein to form the functional center of an RNA-induced silencing complex (RISC). In humans, Argonaute2 (AGO2) assembles with the guide RNA-generating enzyme Dicer and the RNA-binding protein TRBP to form a RISC-loading complex (RLC), which is necessary for efficient transfer of nascent siRNAs and miRNAs from Dicer to AGO2. Here, using single-particle EM analysis, we show that human Dicer has an L-shaped structure. The RLC Dicer's N-terminal DExH/D domain, located in a short 'base branch', interacts with TRBP, whereas its C-terminal catalytic domains in the main body are proximal to AGO2. A model generated by docking the available atomic structures of Dicer and Argonaute homologs into the RLC reconstruction suggests a mechanism for siRNA transfer from Dicer to AGO2

Fluorescent probes of the orientation of myosin regulatory light chains in relaxed, rigor, and contracting muscle.

The orientation of the light-chain region of myosin heads in relaxed, rigor, and isometrically contracting fibers from rabbit psoas muscle was studied by fluorescence polarization. Cysteine 108 of chicken gizzard myosin regulatory light chain (cgRLC) was covalently modified with iodoacetamidotetramethylrhodamine (iodo-ATR). Native RLC of single glycerinated muscle fibers was exchanged for labeled cgRLC in a low [Mg2+] rigor solution at 30 degrees C. Troponin and troponin C removed in this procedure were replaced. RLC exchange had little effect on active force production. X-ray diffraction showed normal structure in rigor after RLC exchange, but loss of axial and helical order in relaxation. In isolated myofibrils labeled cgRLC was confined to the regions of the sarcomere containing myosin heads. The ATR dipoles showed a preference for orientations perpendicular to the fiber axis, combined with limited nanosecond rotational motion, in all conditions studied. The perpendicular orientation preference was more marked in rigor than in either relaxation or active contraction. Stretching relaxed fibers to sarcomere length 4 microns to eliminate overlap between actin- and myosin-containing filaments had little effect on the orientation preference. There was no change in orientation preference when fibers were put into rigor at sarcomere length 4.0 microns. Qualitatively similar results were obtained with ATR-labeled rabbit skeletal RLC

Characterizing Inductive and Capacitive Nonlinear RLC Circuits: A Passivity Test

Linear time-invariant RLC circuits are said to be inductive (capacitive) if the current waveform in sinusoidal steady-state has a negative (resp., positive) phase shift with respect to the voltage. Furthermore, it is known that the circuit is inductive (capacitive) if and only if the magnetic energy stored in the inductors dominates (resp., is dominated by) the electrical energy stored in the capacitors. In this paper we propose a framework, based on passivity theory, that allows to extend these intuitive notions to nonlinear RLC circuits.

Arbuscular mycorrhizas in phosphate-polluted soil: interrelations between root colonization and nitrogen

To investigate whether arbuscular mycorrhizal fungi (AMF) – abundant in a phosphate-polluted but nitrogen-poor field site – improve plant N nutrition, we carried out a two-factorial experiment, including N fertilization and fungicide treatment. Percentage of root length colonized (% RLC) by AMF and tissue element concentrations were determined for four resident plant species. Furthermore, soil nutrient levels and N effects on aboveground biomass of individual species were measured. Nitrogen fertilization lowered % RLC by AMF of Artemisia vulgaris L., Picris hieracioides L. and Poa compressa L., but not of Bromus japonicus Thunb. This – together with positive N addition effects on N status, N:P-ratio and aboveground biomass of most species – suggested that plants are mycorrhizal because of N deficiency. Fungicide treatment, which reduced % RLC in all species, resulted in lower N concentrations in A. vulgaris and P. hieracioides, a higher N concentration in P. compressa, and did not consistently affect N status of B. japonicus. Evidently, AMF had an influence on the N nutrition of plants in this P-rich soil; however – potentially due to differences in their mycorrhizal responsiveness – not all species seemed to benefit froma mycorrhiza-mediated N uptake and accordingly, N distribution