Multicarrier communication systems with low sensibility to nonlinear amplification

Actualment estem entrant a una nova era de la informació amb gran demanda de sistemes de comunicació sense fils. Nous serveis com dades i video requereixen transmissions fiables d'alta velocitat, fins i tot en escenaris d'alta mobilitat. A més a més, la dificultat d'assignar el limitat espectre radioelèctric juntament amb la necessitat d'incrementar el temps de vida de les bateries dels terminals mòbils, requereix el diseny de transceptors que usin la potència i l'ampla de banda disponibles de manera eficient. Les comunicacions multiportadora basades en OFDM són capaces de satisfer la majoria d'aquests requeriments. Però, entre altres reptes, reduir la sensibilitat a la amplificació no-lineal és un factor clau durant el diseny. En aquesta tesi doctoral s'analitza la sensibilitat dels sistemes multiportadora basats en OFDM a l'amplificació no-lineal i es consideren formes eficients per superar aquest problema. La tesi s'enfoca principalment al problema de reduir les fluctuacions de l'envolupant del senyal transmès. En aquest sentit es presenta també un estudi de les mètriques de l'envolupant del senyal, PAPR i CM. A més a més, basant-nos en l'anàlisis presentat es proposen noves tècniques per sistemes OFDM i MC-SS. Per MC-SS, també es tracta el diseny d'una tècnica de postprocessament en forma de detector multiusuari per canals no-lineals.Actualmente estamos entrando en una nueva era de la información donde se da una gran demanda de sistemas de comunicación inalámbricos. Nuevos servicios como datos y vídeo requieren transmisiones fiables de alta velocidad, incluso en escenarios de alta movilidad. Además, la dificultad de asignar el limitado espectro radioeléctrico junto con la necesidad de incrementar el tiempo de vida de las baterías de los terminales móviles, requiere el diseño de transceptores que usen eficientemente la potencia y el ancho de banda disponibles. Las comunicaciones multiportadora basadas en OFDM son capaces de satisfacer la mayoría de dichos requerimientos. Sin embargo, entre otros retos, reducir su sensibilidad a la amplificación no-lineal es un factor clave durante el diseño. En esta tesis se analiza la sensibilidad de los sistemas multiportadora basados en OFDM a la amplificación no-lineal y se consideran formas eficientes para superar dicho problema. La tesis se enfoca principalmente al problema de reducir las fluctuaciones de la envolvente. En este sentido también se presenta un estudio de las métricas de la señal, PAPR y CM. Además, basándonos en el análisis presentado se proponen nuevas técnicas para OFDM y MC-SS. Para MC-SS, también se trata el diseño de un detector multiusuario para canales no-lineales.We are now facing a new information age with high demand of wireless communication systems. New services such as data and video require achieving reliable high-speed transmissions even in high mobility scenarios. Moreover, the difficulty to allocate so many wireless communication systems in the limited frequency band in addition to the demand for long battery life requires designing spectrum and power efficient transceivers. Multicarrier communications based on OFDM are known to fulfill most of the requirements of such systems. However, among other challenges, reducing the sensitivity to nonlinear amplification has become a design key. In this thesis the sensitivity of OFDM-based multicarrier systems to nonlinear amplification is analyzed and efficient ways to overcome this problem are considered. The focus is mainly on the problem of reducing the envelope fluctuations. Therefore, a study of the signal metrics, namely PAPR and CM, is also presented. From the presented analysis, several new techniques for OFDM and MC-SS are proposed. For MC-SS, the design of a post-processing technique in the form of a multiuser detector for nonlinearly distorted MC-SS symbols is also addressed

Single Layer Graphene Biointerface: Studying Neuronal Network Development and Monitoring Cell Behavior over Time

The objective of my Ph.D. thesis is the investigation of the role of Single Layer Graphene (SLG) as a biointerface for its possible future exploitation in various biomedical applications; in particular for the development of biosensors, substrates for regenerative medicine, interfacing platforms for better recording of electrophysiological activity of neuronal networks, among others. This Ph.D. project is multidisciplinary involving both the material transfer and characterization part from one side and the biological part from another side. The material part offers an in-depth explanation of SLG synthesis, transfer, characterization and functionalization while the biological section sheds light on the studies performed for investigation of the behavior of different types of cell lines on SLG substrates. For better understanding of the sequence of the performed work, I have divided this thesis into separate chapters. In the beginning and end of every chapter, I added an introduction and conclusions related to it. Chapter 1 acts as a general introduction to graphene and graphene-related materials where a detailed explanation on the evolution of those materials as a cell interface is provided leading to the introduction of SLG in the end of this chapter along with its production process. Chapter 2 is oriented on the surface characterization of SLG substrates; in this chapter, I described the SLG transfer method, creation of the micrometric ablated geometric patterns on the transferred substrates using excimer laser micromachining, a technique developed in our lab, then further functionalization of the substrates and finally all the techniques employed for their physicochemical characterization. Chapter 3 is dedicated to the biological part of the project; i.e. studying the behavior of different cell lines on the SLG substrates. In this chapter, I have described and explained the interest of using the selected cell lines and the experiments that were performed on them. Chapter 4 has been devoted to a complete and separate project that I performed in collaboration with the Neuroscience and Brain Technologies department. The main focus of the project was the functionalization of the commercial multi-electrode arrays (MEAs) with SLG and studying the neuronal network activity on them throughout the complete network development. Although the main focus of my Ph.D. project was studying SLG biointerface, I have also been involved in side projects, among which, studying the neuronal-like response of mouse neuroblastoma (N2a) living cells to nanoporous patterns of thin supported anodic alumina which I have described in Appendix A, and studying the surface potential of graphene by polyelectrolyte coating which I have presented in Appendix B. To summarize, this thesis reports an original investigation, since, to the best of our knowledge, there is no report yet about the study of the effect of SLG functionalized MEA on the neuronal network activity throughout the complete network maturation. Furthermore, proliferation curves of different cell lines on SLG versus control substrates have been presented; in addition to physicochemical characterization of ablated and functionalized SLG substrates as means of possible explanation of a certain cellular behavior on graphene

Star poly(ε-caprolactone)-based electrospun fibers as biocompatible scaffold for doxorubicin with prolonged drug release activity

Abstract In this work, a novel drug delivery system consisting of poly(e-caprolactone) (PCL) electrospun fibers containing an ad-hoc-synthesized star polymer made up of a poly(amido-amine) (PAMAM) core and PCL branches (PAMAM-PCL) was developed. The latter system which was synthesized via the ring opening polymerization of e-caprolactone, starting from a hydroxyl-terminated PAMAM dendrimer and characterized by means of 1H NMR, IR and DSC, was found to be compatible with both the polymer matrix and a hydrophilic chemotherapeutic drug, doxorubicin (DOXO), the model drug used in this work. The preparation of the dendritic PCL star product with an average arm length of 2000 g/mol was characterized using IR and 1H NMR measurements. The prepared star polymer possessed a higher crystallinity and a lower melting temperature than that of the used linear PCL. Electrospun fibers were prepared starting from solutions containing the neat PCL as well as the PCL/PAMAM-PCL mixture. Electrospinning conditions were optimized in order to obtain defect free fibers, which was proven by the structural FE-SEM study. PAMAM moieties enhanced the hydrophilicity of the fibers, as proved by comparing the water absorption for the PCL/PAMAM-PCL fibers to that neat PCL fibers. The drug-loaded system PCL/PAMAM-PCL was prepared by directly introducing DOXO into the electrospinning solutions. The DOXO-loaded PCL/PAMAM-PCL showed a prolonged release of the drug with respect to the DOXO-loaded PCL fibers and elicited effective controlled toxicity over A431 epidermoid carcinoma, HeLa cervical cancer cells and drug resistant MCF-7 breast cancer cells. On the contrary, the drug-free PCL/PAMAM-PCL scaffold demonstrated no toxic effects on human dermal fibroblasts, suggesting the biocompatibility of the proposed system which can be used in cellular scaffold applications

The Deep Space Network: A Radio Communications Instrument for Deep Space Exploration

The primary purpose of the Deep Space Network (DSN) is to serve as a communications instrument for deep space exploration, providing communications between the spacecraft and the ground facilities. The uplink communications channel provides instructions or commands to the spacecraft. The downlink communications channel provides command verification and spacecraft engineering and science instrument payload data

Proceedings of the Second International Mobile Satellite Conference (IMSC 1990)

Presented here are the proceedings of the Second International Mobile Satellite Conference (IMSC), held June 17-20, 1990 in Ottawa, Canada. Topics covered include future mobile satellite communications concepts, aeronautical applications, modulation and coding, propagation and experimental systems, mobile terminal equipment, network architecture and control, regulatory and policy considerations, vehicle antennas, and speech compression

Graphene-Based Nanomaterials for Neuroengineering: Recent Advances and Future Prospective

Graphene unique physicochemical properties made it prominent among other allotropic forms of carbon, in many areas of research and technological applications. Interestingly, in recent years, many studies exploited the use of graphene family nanomaterials (GNMs) for biomedical applications such as drug delivery, diagnostics, bioimaging, and tissue engineering research. GNMs are successfully used for the design of scaffolds for controlled induction of cell differentiation and tissue regeneration. Critically, it is important to identify the more appropriate nano/bio material interface sustaining cells differentiation and tissue regeneration enhancement. Specifically, this review is focussed on graphene-based scaffolds that endow physiochemical and biological properties suitable for a specific tissue, the nervous system, that links tightly morphological and electrical properties. Different strategies are reviewed to exploit GNMs for neuronal engineering and regeneration, material toxicity, and biocompatibility. Specifically, the potentiality for neuronal stem cells differentiation and subsequent neuronal network growth as well as the impact of electrical stimulation through GNM on cells is presented. The use of field effect transistor (FET) based on graphene for neuronal regeneration is described. This review concludes the important aspects to be controlled to make graphene a promising candidate for further advanced application in neuronal tissue engineering and biomedical use

Ultra-sensitive bioelectronic transducers for extracellular electrophysiological studies

Extracellular electrical activity of cells is commonly recorded using microelectrode arrays (MEA) with planar electrodes. MEA technology has been optimized to record signals generated by excitable cells such as neurons. These cells produce spikes referred to as action potentials. However, all cells produce membrane potentials. In contrast to action potentials, electrical signals produced by non-excitable or non-electrogenic cells, do not exhibit spikes, rather smooth potentials that can change over periods of several minutes with amplitudes of only a few microvolts. These bioelectric signals serve functional roles in signalling pathways that control cell proliferation, differentiation and migration. Measuring and understanding these signals is of high priority in developmental biology, regenerative medicine and cancer research. The objective of this thesis is to fabricate and characterise bioelectronic transducers to measure in vitro the bioelectrical activity of non-electrogenic cells. Since these signals are in the order of few microvolts the electrodes must have an unrivaled low detection limit in the order of hundreds of nanovolts. To meet this challenge a methodology to analyze how bioelectrical signals are coupled into sensing surfaces was developed. The methodology relies on a description of the sensing interface by an equivalent circuit. Procedures for circuit parameter extraction are presented. Relation between circuit parameters, material properties and geometrical design was established. This knowledge was used to establish guidelines for device optimization. The methodology was first used to interpret recordings using gold electrodes, later it as extended to conducting polymers surfaces (PEDOT:PSS ) and finally to graphene electrolyte-gated transistors. The results of this thesis have contributed to the advance of the knowledge in bioelectronic transducers in the following aspects: (i) Detection of signals produced by an important class of neural cells, astrocyte and glioma that thus far had remained inaccessible using conventional extracellular electrodes. (ii) Development of an electrophysiological quantitative method for in vitro monitoring of cancer cell migration and cell-to-cell connections. (iii)An understanding of the limitations of electrolyte-gated transistors to record high frequency signals.A atividade elétrica extracelular das células é geralmente medida usando matrizes de micro-elétrodos (MEA) planares. A tecnologia MEA foi otimizada para medir sinais gerados por células excitáveis, como os neurónios. Essas células produzem sinais conhecidos como potenciais de ação. No entanto, todas as células produzem potenciais de membrana. Em contraste com os potenciais de ação, os sinais elétricos gerados por células não excitáveis ou não eletrogénicas, não são “spikes”, mas sinais que variam lentamente e que podem mudar ao longo de períodos de vários minutos com amplitudes de apenas alguns microvolts. Estes sinais desempenham funções importantes nos mecanismos de sinalização que controlam a proliferação, a diferenciação e a migração celular. Medir e entender esses sinais é importante na biologia do desenvolvimento, na medicina regenerativa e no desenvolvimento de novas terapias para combater células cancerosas. O objetivo desta tese é fabricar e caracterizar transdutores para medir in vitro a atividade de células não eletrogénicas. Como esses sinais são da ordem de alguns microvolts, os elétrodos devem ter um limite de detecção na ordem de centenas de nanovolts. Para enfrentar este desafio, foi desenvolvida uma metodologia para analisar a forma como os sinais se acoplam à superfície do sensor. A metodologia baseia-se na descrição da interface de detecção por um circuito eléctrico equivalente. Procedimentos para extração dos parâmetros de circuito e a relação com as propriedades do material e o desenho geométrico foi estabelecida. Este conhecimento foi usado para estabelecer diretrizes para otimização dos transdutores. Em primeiro lugar a metodologia foi usada para interpretar as medidas de sinais usando elétrodos de ouro, posteriormente estendida para analisar superfícies de polímeros condutores (PEDOT: PSS) e, finalmente, para compreender o funcionamento de transístores. Os resultados desta tese contribuíram para o avanço do conhecimento em transdutores bioeletrónicos nos seguintes aspectos: (i) Detecção de sinais produzidos por uma importante classe de células neurais, astrócitos e gliomas, que tem permanecido inacessíveis usando elétrodos extracelulares. (ii) Desenvolvimento de um método eletrofisiológico para medir a migração de células cancerosas e o estabelecimento de conexões entre células. (ii) Estudo das limitações dos transístores para medir sinais eletrofisiológicos rápidos.The work developed in this thesis was carried out within the framework of the project entitled: “Implantable Organic Devices for Advanced Therapies (INNOVATE)”, ref. PTDC/EEI-AUT/5442/2014, financed by Fundação para a Ciência e Tecnologia (FCT).This project was carried out at the laboratories of the “ Instituto de Telecomunicações (IT) UID/Multi/04326/2013” at the University of the Algarve. The PhD study period received full scholarship under European EM program, “Erasmus Mundus Action 2 (EMA2)” coordinated by University of Warsaw

Structural Characterization of Atomically Thin Hexagonal Boron Nitride via Raman Spectroscopy

A non-destruction evaluation of atomically thin hexagonal boron nitride (h-BN) films is critical to the U.S. Air Force and Department of Defense initiatives pursuing graphene-based electronic field effect transistors (FETs) capable of operating at terahertz frequencies. H-BN thin films an increase to the characteristic E2g 1367cm-1 h-BN peak intensity has been correlated to an increase in film thickness. Raman spectroscopy on a h-BN film with thicknesses of 7, 14, and 21 atoms (2.5nm, 5nm, 7.5nm respectively) revealed a linear relationship between peak intensity and thickness. This relationship can mathematically be described as y=0.0265x+0.8084, and fits the data with a R2 value of 0.9986. There was no observed correlation between film thickness and full width at half maximum (FWHM) and there was no measured shift to the E2g peak with increasing film thickness