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Spectral imaging in preclinical research and clinical pathology.
Spectral imaging methods are attracting increased interest from researchers and practitioners in basic science, pre-clinical and clinical arenas. A combination of better labeling reagents and better optics creates opportunities to detect and measure multiple parameters at the molecular and cellular level. These tools can provide valuable insights into the basic mechanisms of life, and yield diagnostic and prognostic information for clinical applications. There are many multispectral technologies available, each with its own advantages and limitations. This chapter will present an overview of the rationale for spectral imaging, and discuss the hardware, software and sample labeling strategies that can optimize its usefulness in clinical settings
Automated Segmentation of Cells with IHC Membrane Staining
This study presents a fully automated membrane segmentation technique for immunohistochemical tissue images with membrane staining, which is a critical task in computerized immunohistochemistry (IHC). Membrane segmentation is particularly tricky in immunohistochemical tissue images because the cellular membranes are visible only in the stained tracts of the cell, while the unstained tracts are not visible. Our automated method provides accurate segmentation of the cellular membranes in the stained tracts and reconstructs the approximate location of the unstained tracts using nuclear membranes as a spatial reference. Accurate cell-by-cell membrane segmentation allows per cell morphological analysis and quantification of the target membrane proteins that is fundamental in several medical applications such as cancer characterization and classification, personalized therapy design, and for any other applications requiring cell morphology characterization. Experimental results on real datasets from different anatomical locations demonstrate the wide applicability and high accuracy of our approach in the context of IHC analysi
Future perspectives of digital pathology
Technological advances have enabled innovative solutions to be achieved in pathology based on digital imaging, now superseding those of conventional microscopy. Digital pathology has been defined as ‘virtual microscopy’ and depends on computer-generated digital imaging of microscope slides (WSI — whole slide imaging) which are in turn created, reviewed, managed, shared, analysed and interpreted. Such WSI systems and digital consulting platforms are now used for teaching, scientific research, telepathology / teleconsultation and diagnostics. They also permit easy and interactive sharing of WSI that can be integrated into other medical information systems. The software for automated image analysis and computer aided diagnosis can thereby make highly accurate diagnoses and help standardise study findings. Despite the technique’s many advantages, its noted drawbacks include high equipment and software costs, image quality issues of standardisation and most importantly, that pathologists are reluctant to use it routinely for making diagnoses
Hybrid Intelligent System for Diagnosing Breast Pre-Cancerous and Cancerous Conditions Based on Image Analysis
Modern diagnostic technologies are automated microscopy systems (AMSs). In this research study, the authors analyzed the modern AMS methods and algorithms. Criteria-based comparative analysis of AMS has been made, and their advantages and disadvantages have been identified at the three main levels of image processing. This allowed determining the main direction of such systems development, that is, designing the hybrid intelligent AMS. The work of an expert physician implies visual image interpretation, selection of qualitative features of micro-objects, the formation of diagnostic rules based on expert knowledge, and making diagnoses. Knowledge introduction model contains a productive model, in which knowledge is presented in the form of rules expressed in productive pseudo code if-then. Logic inference machine is a module designed to logically derive the facts and rules from the base according to the laws of formal logic. A set of modern methods and algorithms for low-, mid-, and high-level image processing have been used in the AMS structure
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