The Filament Sensor for Near Real-Time Detection of Cytoskeletal Fiber Structures

A reliable extraction of filament data from microscopic images is of high interest in the analysis of acto-myosin structures as early morphological markers in mechanically guided differentiation of human mesenchymal stem cells and the understanding of the underlying fiber arrangement processes. In this paper, we propose the filament sensor (FS), a fast and robust processing sequence which detects and records location, orientation, length and width for each single filament of an image, and thus allows for the above described analysis. The extraction of these features has previously not been possible with existing methods. We evaluate the performance of the proposed FS in terms of accuracy and speed in comparison to three existing methods with respect to their limited output. Further, we provide a benchmark dataset of real cell images along with filaments manually marked by a human expert as well as simulated benchmark images. The FS clearly outperforms existing methods in terms of computational runtime and filament extraction accuracy. The implementation of the FS and the benchmark database are available as open source.Comment: 32 pages, 21 figure

Human Crowdsourcing Data for Indoor Location Applied to Ambient Assisted Living Scenarios

In the last decades, the rise of life expectancy has accelerated the demand for new technological solutions to provide a longer life with improved quality. One of the major areas of the Ambient Assisted Living aims to monitor the elderly location indoors. For this purpose, indoor positioning systems are valuable tools and can be classified depending on the need of a supporting infrastructure. Infrastructure-based systems require the investment on expensive equipment and existing infrastructure-free systems, although rely on the pervasively available characteristics of the buildings, present some limitations regarding the extensive process of acquiring and maintaining fingerprints, the maps that store the environmental characteristics to be used in the localisation phase. These problems hinder indoor positioning systems to be deployed in most scenarios. To overcome these limitations, an algorithm for the automatic construction of indoor floor plans and environmental fingerprints is proposed. With the use of crowdsourcing techniques, where the extensiveness of a task is reduced with the help of a large undefined group of users, the algorithm relies on the combination ofmultiple sources of information, collected in a non-annotated way by common smartphones. The crowdsourced data is composed by inertial sensors, responsible for estimating the users’ trajectories, Wi-Fi radio and magnetic field signals. Wi-Fi radio data is used to cluster the trajectories into smaller groups, each corresponding to specific areas of the building. Distance metrics applied to magnetic field signals are used to identify geomagnetic similarities between different users’ trajectories. The building’s floor plan is then automatically created, which results in fingerprints labelled with physical locations. Experimental results show that the proposed algorithm achieved comparable floor plan and fingerprints to those acquired manually, allowing the conclusion that is possible to automate the setup process of infrastructure-free systems. With these results, this solution can be applied in any fingerprinting-based indoor positioning system

Fingerabdruckswachstumvorhersage, Bildvorverarbeitung und Multi-level Judgment Aggregation

Im ersten Teil dieser Arbeit wird Fingerwachstum untersucht und eine Methode zur Vorhersage von Wachstum wird vorgestellt. Die Effektivität dieser Methode wird mittels mehrerer Tests validiert. Vorverarbeitung von Fingerabdrucksbildern wird im zweiten Teil behandelt und neue Methoden zur Schätzung des Orientierungsfelds und der Ridge-Frequenz sowie zur Bildverbesserung werden vorgestellt: Die Line Sensor Methode zur Orientierungsfeldschätzung, gebogene Regionen zur Ridge-Frequenz-Schätzung und gebogene Gabor Filter zur Bildverbesserung. Multi-level Jugdment Aggregation wird eingeführt als Design Prinzip zur Kombination mehrerer Methoden auf mehreren Verarbeitungsstufen. Schließlich wird Score Neubewertung vorgestellt, um Informationen aus der Vorverarbeitung mit in die Score Bildung einzubeziehen. Anhand eines Anwendungsbeispiels wird die Wirksamkeit dieses Ansatzes auf den verfügbaren FVC-Datenbanken gezeigt.Finger growth is studied in the first part of the thesis and a method for growth prediction is presented. The effectiveness of the method is validated in several tests. Fingerprint image preprocessing is discussed in the second part and novel methods for orientation field estimation, ridge frequency estimation and image enhancement are proposed: the line sensor method for orientation estimation provides more robustness to noise than state of the art methods. Curved regions are proposed for improving the ridge frequency estimation and curved Gabor filters for image enhancement. The notion of multi-level judgment aggregation is introduced as a design principle for combining different methods at all levels of fingerprint image processing. Lastly, score revaluation is proposed for incorporating information obtained during preprocessing into the score, and thus amending the quality of the similarity measure at the final stage. A sample application combines all proposed methods of the second part and demonstrates the validity of the approach by achieving massive verification performance improvements in comparison to state of the art software on all available databases of the fingerprint verification competitions (FVC)

Multimodal Biometrics Enhancement Recognition System based on Fusion of Fingerprint and PalmPrint: A Review

This article is an overview of a current multimodal biometrics research based on fingerprint and palm-print. It explains the pervious study for each modal separately and its fusion technique with another biometric modal. The basic biometric system consists of four stages: firstly, the sensor which is used for enrolmen

Automated extraction of chemical structure information from digital raster images

Background: To search for chemical structures in research articles, diagrams or text representing molecules need to be translated to a standard chemical file format compatible with cheminformatic search engines. Nevertheless, chemical information contained in research articles is often referenced as analog diagrams of chemical structures embedded in digital raster images. To automate analog-to-digital conversion of chemical structure diagrams in scientific research articles, several software systems have been developed. But their algorithmic performance and utility in cheminformatic research have not been investigated. Results: This paper aims to provide critical reviews for these systems and also report our recent development of ChemReader -- a fully automated tool for extracting chemical structure diagrams in research articles and converting them into standard, searchable chemical file formats. Basic algorithms for recognizing lines and letters representing bonds and atoms in chemical structure diagrams can be independently run in sequence from a graphical user interface-and the algorithm parameters can be readily changed-to facilitate additional development specifically tailored to a chemical database annotation scheme. Compared with existing software programs such as OSRA, Kekule, and CLiDE, our results indicate that ChemReader outperforms other software systems on several sets of sample images from diverse sources in terms of the rate of correct outputs and the accuracy on extracting molecular substructure patterns. Conclusion: The availability of ChemReader as a cheminformatic tool for extracting chemical structure information from digital raster images allows research and development groups to enrich their chemical structure databases by annotating the entries with published research articles. Based on its stable performance and high accuracy, ChemReader may be sufficiently accurate for annotating the chemical database with links to scientific research articles.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90875/1/Saitou8.pd

Intelligent indexing of crime scene photographs

The Scene of Crime Information System's automatic image-indexing prototype goes beyond extracting keywords and syntactic relations from captions. The semantic information it gathers gives investigators an intuitive, accurate way to search a database of cases for specific photographic evidence. Intelligent, automatic indexing and retrieval of crime scene photographs is one of the main functions of SOCIS, our research prototype developed within the Scene of Crime Information System project. The prototype, now in its final development and evaluation phase, applies advanced natural language processing techniques to text-based image indexing and retrieval to tackle crime investigation needs effectively and efficiently