Simultaneous evaluation of multiple microarray surface chemistries through real-time interferometric imaging.

Surface chemistry is a crucial aspect for microarray modality biosensor development. The immobilization capability of the functionalized surface is indeed a limiting factor for the final yield of the binding reaction. In this work, we were able to simultaneously compare the functionality of protein ligands that were locally immobilized on different polymers, while on the same solid support, therefore demonstrating a new way of multiplexing. Our goal was to investigate, in a single experiment, both the immobilization efficiency of a group of reactive polymers and the resulting affinity of the tethered molecules. This idea was demonstrated by spotting many reactive polymers on a Si/SiO2 chip and depositing the molecular probes on the spots immediately after. As a proof of concept, we focused on which polymers would better immobilize a model protein (α-Lactalbumin) and a peptide (LAC-1). We successfully showed that this protocol is applicable to proteins and peptides with a good efficiency. By means of real-time binding measurements performed with the interferometric reflectance imaging sensor (IRIS), local functionalization proved to be comparable to the classical flat coating solution. The final outcome highlights the multiplexing power of this method: first, it allows to characterize dozens of polymers at once. Secondly, it removes the limitation, related to coated surfaces, that only molecules with the same functional groups can be tethered to the same solid support. By applying this protocol, many types of molecules can be studied simultaneously and immobilization for each probe can be individually optimized.766466 (INDEX) - Horizon 2020 Framework Programmehttps://s3-eu-west-1.amazonaws.com/itempdf74155353254prod/8976347/Simultaneous_Evaluation_of_Multiple_Microarray_Surface_Chemistries_Through_Real-Time_Interferometric_Imaging_v1.pdfFirst author draf

A Differential Approach for Gaze Estimation

Non-invasive gaze estimation methods usually regress gaze directions directly from a single face or eye image. However, due to important variabilities in eye shapes and inner eye structures amongst individuals, universal models obtain limited accuracies and their output usually exhibit high variance as well as biases which are subject dependent. Therefore, increasing accuracy is usually done through calibration, allowing gaze predictions for a subject to be mapped to his/her actual gaze. In this paper, we introduce a novel image differential method for gaze estimation. We propose to directly train a differential convolutional neural network to predict the gaze differences between two eye input images of the same subject. Then, given a set of subject specific calibration images, we can use the inferred differences to predict the gaze direction of a novel eye sample. The assumption is that by allowing the comparison between two eye images, annoyance factors (alignment, eyelid closing, illumination perturbations) which usually plague single image prediction methods can be much reduced, allowing better prediction altogether. Experiments on 3 public datasets validate our approach which constantly outperforms state-of-the-art methods even when using only one calibration sample or when the latter methods are followed by subject specific gaze adaptation.Comment: Extension to our paper A differential approach for gaze estimation with calibration (BMVC 2018) Submitted to PAMI on Aug. 7th, 2018 Accepted by PAMI short on Dec. 2019, in IEEE Transactions on Pattern Analysis and Machine Intelligenc

PIONIER: a visitor instrument for the VLTI

PIONIER is a 4-telescope visitor instrument for the VLTI, planned to see its first fringes in 2010. It combines four ATs or four UTs using a pairwise ABCD integrated optics combiner that can also be used in scanning mode. It provides low spectral resolution in H and K band. PIONIER is designed for imaging with a specific emphasis on fast fringe recording to allow closure-phases and visibilities to be precisely measured. In this work we provide the detailed description of the instrument and present its updated status.Comment: Proceedings of SPIE conference Optical and Infrared Interferometry II (Conference 7734) San Diego 201

The continuous rise of bulges out of galactic disks

(abridged) This study revolves around dmB, a new distance- and extinction-independent measure of the contribution by stellar populations older than 9 Gyr to the mean r-band surface brightness of the bulge component in 135 late-type galaxies (LTGs) from the CALIFA survey, spanning a range of 2.6 dex and 3 dex in total and bulge stellar mass (M*T~10^(8.9-11.5) M_solar and M*B~10^(8.3-11.3) M_solar, respectively). The main insight from this study is that LTG bulges form a continuous sequence of increasing dmB with increasing M*T, M*B, stellar mass surface density S* and mass-weighted age and metallicity: high-dmB bulges are the oldest, densest and most massive ones, and vice versa. Furthermore, we find that the bulge-to-disk age and metallicity contrast, as well as the bulge-to-disk mass ratio increase with M*T, raising from, respectively, ~0 Gyr, 0 dex and 0.25 to ~3 Gyr, ~0.3 dex and 0.67 across the mass range covered by our sample. Whereas gas excitation in lower-mass bulges is invariably dominated by star formation (SF), LINER- and Seyfert-specific emission-line ratios were exclusively documented in high-mass, high-S* bulges. The continuity both in the properties of LTG bulges themselves and in their age and metallicity contrast to their parent disks suggests that these components evolve alongside in a concurrent process that leads to a continuum of physical and evolutionary characteristics. Our results are consistent with a picture where bulge growth in LTGs is driven by a superposition of quick-early and slow-secular processes, the relative importance of which increases with M*T. These processes, which presumably combine in situ SF in the bulge and inward migration of material from the disk, are expected to lead to a non-homologous radial growth of S* and a trend for an increasing Sersic index with increasing galaxy mass.Comment: 24 pages, accepted for publication in A&