The plant-pathogen haustorial interface at a glance

Many filamentous pathogens invade plant cells through specialized hyphae called haustoria. These infection structures are enveloped by a newly synthesized plant-derived membrane called the extrahaustorial membrane (EHM). This specialized membrane is the ultimate interface between the plant and pathogen, and is key to the success or failure of infection. Strikingly, the EHM is reminiscent of host-derived membrane interfaces that engulf intracellular metazoan parasites. These perimicrobial interfaces are critical sites where pathogens facilitate nutrient uptake and deploy virulence factors to disarm cellular defenses mounted by their hosts. Although the mechanisms underlying the biogenesis and functions of these host-microbe interfaces are poorly understood, recent studies have provided new insights into the cellular and molecular mechanisms involved. In this Cell Science at a Glance and the accompanying poster, we summarize these recent advances with a specific focus on the haustorial interfaces associated with filamentous plant pathogens. We highlight the progress in the field that fundamentally underpin this research topic. Furthermore, we relate our knowledge of plant-filamentous pathogen interfaces to those generated by other plant-associated organisms. Finally, we compare the similarities between host-pathogen interfaces in plants and animals, and emphasize the key questions in this research area

Molecular Structure and Modeling of Water-Air and Ice-Air Interfaces Monitored by Sum-Frequency Generation.

From a glass of water to glaciers in Antarctica, water-air and ice-air interfaces are abundant on Earth. Molecular-level structure and dynamics at these interfaces are key for understanding many chemical/physical/atmospheric processes including the slipperiness of ice surfaces, the surface tension of water, and evaporation/sublimation of water. Sum-frequency generation (SFG) spectroscopy is a powerful tool to probe the molecular-level structure of these interfaces because SFG can specifically probe the topmost interfacial water molecules separately from the bulk and is sensitive to molecular conformation. Nevertheless, experimental SFG has several limitations. For example, SFG cannot provide information on the depth of the interface and how the orientation of the molecules varies with distance from the surface. By combining the SFG spectroscopy with simulation techniques, one can directly compare the experimental data with the simulated SFG spectra, allowing us to unveil the molecular-level structure of water-air and ice-air interfaces. Here, we present an overview of the different simulation protocols available for SFG spectra calculations. We systematically compare the SFG spectra computed with different approaches, revealing the advantages and disadvantages of the different methods. Furthermore, we account for the findings through combined SFG experiments and simulations and provide future challenges for SFG experiments and simulations at different aqueous interfaces

Nanoanalysis of a sub-nanometre reaction layer in a metal inserted high-k gate stack

Reactions at the interfaces can occur in metal inserted high-k gate stacks and are likely to evolve during device processing. Such reactions may affect the electrical properties of the stack and hence these could change during processing. The key interfaces are often not atomically flat and characterising the reaction layers on the near atomic scale required is a challenge. Aberration corrected scanning transmission electron microscopy (STEM) and spectrum imaging (SI) using electron energy loss spectroscopy (EELS) is used to characterise an HfN or Hf(O,N) reaction layer, ∼0.25 nm wide, between HfO<sub>2</sub> and TiN. This demonstrates the very significant advances in high spatial resolution characterisation made in recent years

Numerical modeling of elastic waves across imperfect contacts

A numerical method is described for studying how elastic waves interact with imperfect contacts such as fractures or glue layers existing between elastic solids. These contacts have been classicaly modeled by interfaces, using a simple rheological model consisting of a combination of normal and tangential linear springs and masses. The jump conditions satisfied by the elastic fields along the interfaces are called the "spring-mass conditions". By tuning the stiffness and mass values, it is possible to model various degrees of contact, from perfect bonding to stress-free surfaces. The conservation laws satisfied outside the interfaces are integrated using classical finite-difference schemes. The key problem arising here is how to discretize the spring-mass conditions, and how to insert them into a finite-difference scheme: this was the aim of the present paper. For this purpose, we adapted an interface method previously developed for use with perfect contacts [J. Comput. Phys. 195 (2004) 90-116]. This numerical method also describes closely the geometry of arbitrarily-shaped interfaces on a uniform Cartesian grid, at negligible extra computational cost. Comparisons with original analytical solutions show the efficiency of this approach.Comment: to be published in SIAM Journal of Scientific Computing (2006

Resource Oriented Modelling: Describing Restful Web Services Using Collaboration Diagrams

The popularity of Resource Oriented and RESTful Web Services is increasing rapidly. In these, resources are key actors in the interfaces, in contrast to other approaches where services, messages or objects are. This distinctive feature necessitates a new approach for modelling RESTful interfaces providing a more intuitive mapping from model to implementation than could be achieved with non-resource methods. With this objective we propose an approach to describe Resource Oriented and RESTful Web Services based on UML collaboration diagrams. Then use it to model scenarios from several problem domains, arguing that Resource Oriented and RESTful Web Services can be used in systems which go beyond ad-hoc integration. Using the scenarios we demonstrate how the approach is useful for: eliciting domain ontologies; identifying recurring patterns; and capturing static and dynamic aspects of the interface

Combining Boundary-Conforming Finite Element Meshes on Moving Domains Using a Sliding Mesh Approach

For most finite element simulations, boundary-conforming meshes have significant advantages in terms of accuracy or efficiency. This is particularly true for complex domains. However, with increased complexity of the domain, generating a boundary-conforming mesh becomes more difficult and time consuming. One might therefore decide to resort to an approach where individual boundary-conforming meshes are pieced together in a modular fashion to form a larger domain. This paper presents a stabilized finite element formulation for fluid and temperature equations on sliding meshes. It couples the solution fields of multiple subdomains whose boundaries slide along each other on common interfaces. Thus, the method allows to use highly tuned boundary-conforming meshes for each subdomain that are only coupled at the overlapping boundary interfaces. In contrast to standard overlapping or fictitious domain methods the coupling is broken down to few interfaces with reduced geometric dimension. The formulation consists of the following key ingredients: the coupling of the solution fields on the overlapping surfaces is imposed weakly using a stabilized version of Nitsche's method. It ensures mass and energy conservation at the common interfaces. Additionally, we allow to impose weak Dirichlet boundary conditions at the non-overlapping parts of the interfaces. We present a detailed numerical study for the resulting stabilized formulation. It shows optimal convergence behavior for both Newtonian and generalized Newtonian material models. Simulations of flow of plastic melt inside single-screw as well as twin-screw extruders demonstrate the applicability of the method to complex and relevant industrial applications

Collaborative music interaction on tabletops: an HCI approach

With the advent of tabletop interaction, collaborative activities are better supported than they are on single-user PCs because there exists a physical shareable space, and interaction with digital data is more embodied and social. In sound and music computing, collaborative music making has traditionally been done using interconnected networks, but using separated computers. Musical tabletops introduce opportunities of playing in collaboration through sharing physically the same musical interface. However, few tabletop musical interfaces exploit this collaborative potential (e.g. the Reactable). We are interested in looking into how collaboration can be fully supported by means of musical tabletops for music performance in contrast with more traditional settings. We are also looking at whether collective musical engagement can be enhanced by providing more suitable interfaces to collaboration. In HCI and software development, we find an iterative process approach of design and evaluation—where evaluation allows us to identify key issues that can be addressed in the next design iteration of the system. Using a similar iterative approach, we plan to design and evaluate some tabletop musical interfaces. The aim is to understand what design choices can enhance and enrich collaboration and collective musical engagement on these systems. In this paper, we explain the evaluation methodologies we have undertaken in three preliminary pilot studies, and the lessons we have learned. Initial findings indicate that evaluating tabletop musical interfaces is a complex endeavour which requires an approach as close as possible to a real context, with an interdisciplinary approach provided by interaction analysis techniques