Validation of Fast Spectrochemical Screening Methods for the Identification of Counterfeit Pharmaceutical Packaging

Counterfeit pharmaceuticals are an actively developing health and economic threat worldwide. Particularly prevalent are counterfeit pharmaceuticals distributed in emerging nations and through internet pharmacies or e-pharmacies. Although technology has been developed that discourages anti-counterfeiting practices (such as optically variable devices, invisible ink, and track-and-trace technology), it remains somewhat novel and expensive to implement on a widespread scale. In this study, Laser Induced Breakdown Spectroscopy (LIBS) and Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) were proposed as fast and non-invasive tools for the identification of counterfeit pharmaceutical packages. The main objective of this research was to develop and evaluate the capabilities of LIBS and ATR-FTIR to determine chemical differences between counterfeit and authentic pharmaceutical packaging samples. LIBS and ATR-FTIR possess several characteristics that render them suitable for rapid on-site detection. They produce analytical results in less than one minute per sample, with high sensitivity and selectivity, limited sample preparation, and minimal destructivity. The methods were evaluated through the analysis of a dataset of 166 packages (112 counterfeits and 54 authentic sources). The dataset was divided into two main subsets. The first subset was evaluated to identify the informative value of LIBS for fast screening of black barcodes and the carton substrate (100 counterfeit and 35 authentic). The multi-color inks and paper of the second subset was investigated for variation of chemical profiles within and between sources, and the method’s capabilities to distinguish between counterfeits (112) and authentic samples (12). One hundred and twelve counterfeit pharmaceutical cartons were printed from five different sources, mimicking six authentic counterparts. The authentic subset consisted of twelve secondary packages of six common medical products, including packages from the same and different manufacturing lots. The selected products consisted of vasodilators, antivirals, steroids, and other commonly counterfeited pharmaceuticals. Intra-source variation of the counterfeit subset was investigated; it was determined to be sufficiently lower than inter-source variation. False exclusion rates were calculated to be less than 20% for samples originating from the same source (e.g., same package, intra-lots, replicate printouts). Using LIBS, a two-class classification system was used for the combined black barcode ink and paperboard carton spectra (n = 135, 100 counterfeit, 35 authentic packages). As black barcode ink is very common on pharmaceutical packaging, this system was used as a general screening technique to quickly identify a sample as authentic or counterfeit, regardless of counterfeit printing source. In general, the correct classification rates for this set were over 92%. The classification models were established using six machine learning methods: Random Forest, Naïve Bayes, Neural Networks, k-Nearest Neighbors, Quadratic Discriminant Analysis, and Linear Discriminant Analysis. A random split of 60% and 40% of the dataset was applied for training and testing of the classifier algorithms. Principal Component Analysis (PCA) was utilized on the LIBS and ATR-FTIR data for variable reduction purposes. The principal components for each ink type were combined prior to classification. Also, a six-class system was also used to classify the dataset using LIBS, ATR-FTIR, and combined data from both techniques (n = 124, 112 counterfeit, 12 authentic packages). The machine learning methods classified the samples as belonging to one of five counterfeit printing sources or their corresponding authentic counterpart. Seven ink colors (red, blue, yellow, green, brown, pink, black) were analyzed; additionally, in ATR-FTIR, the paperboard substrate was also analyzed. In most comparisons, LIBS had a successful classification rate of over 70% and ATR-FTIR had a successful classification rate of over 85%. When the data from both techniques were combined, the discrimination power of the system increased to 93% correct classification. Although LIBS and ATR-FTIR had a low misclassification rate when used in isolation, the misclassification rate could be reduced even further through data combination. The results of this study are encouraging for the inclusion of LIBS and ATR-FTIR as a screening method for the detection of counterfeit pharmaceutical packaging. The utilization of combined data to discover chemical signatures addresses an urgent need in the investigation of counterfeit pharmaceuticals. Also, the classification of counterfeit samples into their specific counterfeit source may benefit investigators as they make determinations in the counterfeit pharmaceutical packaging supply chain. This study is anticipated to offer relevant tools to both government and pharmaceutical industry in the detection and fight against counterfeit pharmaceuticals

NIR spectroscopy for personal screening

This work presents investigations into the use of the near-infrared (NIR) signals to interrogate, detect and image specific chemical compounds of interest in a security screening application, including when such compounds are hidden behind single layers of clothing fabric. In an initial set of experiments, the mechanisms governing the interaction of NIR signals with clothing fabrics and similar materials has been studied, in order to account for the influence of fabric layers when detecting hidden chemicals. Throughout the rest of the work, NIR spectroscopy has been used as a means to perform qualitative and quantitative analysis, in order to detect the presence of chemicals, and quantify the concentration in aqueous solution of liquids. It has been shown that, while the compounds can be identified on the basis of the characteristic features that appear in the relevant NIR spectra, the origin and nature of these spectra necessitate that such identification be performed with a chemometricsbased approach. Accordingly, multivariate calibration models based on neural networks and partial least squares regression (PLSR) have been developed to perform the requisite analyses. Results of calibration and testing with a range of data are reported. In order to facilitate operation in practical security screening, the development and testing of a software-based lock-in amplifier is reported, as a mean to enhance the signal-to-noise ratio (SNR) of the spectral data. It is shown that the amplifier can process up to 40 wavelength channels in parallel, to extract the spectral data buried in noise in each channel. Hence, with the SNR of the input signal set as low as -60 dB (by introducing software-generated additive white noise in the spectra), adequate noise suppression has been obtained, allowing the resulting spectral data to be used for requisite chemical detection. Finally, an integrated spectroscopic imaging application is developed to perform twodimensional cross-sectional scans of chemical samples, carry out lock-in amplification of the recorded intensity spectra, and plot the results of neural network-based chemical detection in the form of intensity images colour-coded to depict the presence of the pertinent chemicals at the scanned coordinates

The 2nd International Electronic Conference on Applied Sciences

This book is focused on the works presented at the 2nd International Electronic Conference on Applied Sciences, organized by Applied Sciences from 15 to 31 October 2021 on the MDPI Sciforum platform. Two decades have passed since the start of the 21st century. The development of sciences and technologies is growing ever faster today than in the previous century. The field of science is expanding, and the structure of science is becoming ever richer. Because of this expansion and fine structure growth, researchers may lose themselves in the deep forest of the ever-increasing frontiers and sub-fields being created. This international conference on the Applied Sciences was started to help scientists conduct their own research into the growth of these frontiers by breaking down barriers and connecting the many sub-fields to cut through this vast forest. These functions will allow researchers to see these frontiers and their surrounding (or quite distant) fields and sub-fields, and give them the opportunity to incubate and develop their knowledge even further with the aid of this multi-dimensional network

Technology 2001: The Second National Technology Transfer Conference and Exposition, volume 2

Proceedings of the workshop are presented. The mission of the conference was to transfer advanced technologies developed by the Federal government, its contractors, and other high-tech organizations to U.S. industries for their use in developing new or improved products and processes. Volume two presents papers on the following topics: materials science, robotics, test and measurement, advanced manufacturing, artificial intelligence, biotechnology, electronics, and software engineering

Human and Artificial Intelligence

Although tremendous advances have been made in recent years, many real-world problems still cannot be solved by machines alone. Hence, the integration between Human Intelligence and Artificial Intelligence is needed. However, several challenges make this integration complex. The aim of this Special Issue was to provide a large and varied collection of high-level contributions presenting novel approaches and solutions to address the above issues. This Special Issue contains 14 papers (13 research papers and 1 review paper) that deal with various topics related to human–machine interactions and cooperation. Most of these works concern different aspects of recommender systems, which are among the most widespread decision support systems. The domains covered range from healthcare to movies and from biometrics to cultural heritage. However, there are also contributions on vocal assistants and smart interactive technologies. In summary, each paper included in this Special Issue represents a step towards a future with human–machine interactions and cooperation. We hope the readers enjoy reading these articles and may find inspiration for their research activities

State of the art of audio- and video based solutions for AAL

Working Group 3. Audio- and Video-based AAL ApplicationsIt is a matter of fact that Europe is facing more and more crucial challenges regarding health and social care due to the demographic change and the current economic context. The recent COVID-19 pandemic has stressed this situation even further, thus highlighting the need for taking action. Active and Assisted Living (AAL) technologies come as a viable approach to help facing these challenges, thanks to the high potential they have in enabling remote care and support. Broadly speaking, AAL can be referred to as the use of innovative and advanced Information and Communication Technologies to create supportive, inclusive and empowering applications and environments that enable older, impaired or frail people to live independently and stay active longer in society. AAL capitalizes on the growing pervasiveness and effectiveness of sensing and computing facilities to supply the persons in need with smart assistance, by responding to their necessities of autonomy, independence, comfort, security and safety. The application scenarios addressed by AAL are complex, due to the inherent heterogeneity of the end-user population, their living arrangements, and their physical conditions or impairment. Despite aiming at diverse goals, AAL systems should share some common characteristics. They are designed to provide support in daily life in an invisible, unobtrusive and user-friendly manner. Moreover, they are conceived to be intelligent, to be able to learn and adapt to the requirements and requests of the assisted people, and to synchronise with their specific needs. Nevertheless, to ensure the uptake of AAL in society, potential users must be willing to use AAL applications and to integrate them in their daily environments and lives. In this respect, video- and audio-based AAL applications have several advantages, in terms of unobtrusiveness and information richness. Indeed, cameras and microphones are far less obtrusive with respect to the hindrance other wearable sensors may cause to one’s activities. In addition, a single camera placed in a room can record most of the activities performed in the room, thus replacing many other non-visual sensors. Currently, video-based applications are effective in recognising and monitoring the activities, the movements, and the overall conditions of the assisted individuals as well as to assess their vital parameters (e.g., heart rate, respiratory rate). Similarly, audio sensors have the potential to become one of the most important modalities for interaction with AAL systems, as they can have a large range of sensing, do not require physical presence at a particular location and are physically intangible. Moreover, relevant information about individuals’ activities and health status can derive from processing audio signals (e.g., speech recordings). Nevertheless, as the other side of the coin, cameras and microphones are often perceived as the most intrusive technologies from the viewpoint of the privacy of the monitored individuals. This is due to the richness of the information these technologies convey and the intimate setting where they may be deployed. Solutions able to ensure privacy preservation by context and by design, as well as to ensure high legal and ethical standards are in high demand. After the review of the current state of play and the discussion in GoodBrother, we may claim that the first solutions in this direction are starting to appear in the literature. A multidisciplinary 4 debate among experts and stakeholders is paving the way towards AAL ensuring ergonomics, usability, acceptance and privacy preservation. The DIANA, PAAL, and VisuAAL projects are examples of this fresh approach. This report provides the reader with a review of the most recent advances in audio- and video-based monitoring technologies for AAL. It has been drafted as a collective effort of WG3 to supply an introduction to AAL, its evolution over time and its main functional and technological underpinnings. In this respect, the report contributes to the field with the outline of a new generation of ethical-aware AAL technologies and a proposal for a novel comprehensive taxonomy of AAL systems and applications. Moreover, the report allows non-technical readers to gather an overview of the main components of an AAL system and how these function and interact with the end-users. The report illustrates the state of the art of the most successful AAL applications and functions based on audio and video data, namely (i) lifelogging and self-monitoring, (ii) remote monitoring of vital signs, (iii) emotional state recognition, (iv) food intake monitoring, activity and behaviour recognition, (v) activity and personal assistance, (vi) gesture recognition, (vii) fall detection and prevention, (viii) mobility assessment and frailty recognition, and (ix) cognitive and motor rehabilitation. For these application scenarios, the report illustrates the state of play in terms of scientific advances, available products and research project. The open challenges are also highlighted. The report ends with an overview of the challenges, the hindrances and the opportunities posed by the uptake in real world settings of AAL technologies. In this respect, the report illustrates the current procedural and technological approaches to cope with acceptability, usability and trust in the AAL technology, by surveying strategies and approaches to co-design, to privacy preservation in video and audio data, to transparency and explainability in data processing, and to data transmission and communication. User acceptance and ethical considerations are also debated. Finally, the potentials coming from the silver economy are overviewed.publishedVersio

Aerospace medicine and biology: A continuing bibliography with indexes (supplement 341)

This bibliography lists 133 reports, articles and other documents introduced into the NASA Scientific and Technical Information System during September 1990. Subject coverage includes: aerospace medicine and psychology, life support systems and controlled environments, safety equipment, exobiology and extraterrestrial life, and flight crew behavior and performance