Measuring Plant Genetic Diversity Using Inter-Simple Sequence Repeats (ISSRS)

Simple sequence repeats (SSRs) have great utility as they are conserved and present in all eukaryotic genomes. Here we report the use of a simple PCR with fluorescently-labelled primers to amplify inter-SSR markers (ISSRs) for diversity assessments. The use of ISSR markers does not rely upon specific genetic sequence information, or prolonged method development and may be measured rapidly using the automated equipment. The major restriction of the ISSR method is at the analysis stage, as the markers are dominant it is not possible to distinguish heterozygotes as loci. We obtained ISSR data from ca. 60 phenotypically characterised Capsella bursa pastoris L. Medic (shepherds purse)accessions that had been isolated from a diverse mix of arable field sites throughout the UK. We developed mathematical scripts for use with the free statistical software tool R (http://www.rproject.org/), that processed the molecular data in a binary format to estimate genetic diversity (using the Jaccard co-efficient), and that related genotype to the plant phenotypic and environmental (site specific) traits. The methodology established has the power to predict the relationship between environmental and plant morphological characteristics

Development of free sugar white chocolate, suitable for diabetics, using Stevia and sucralose as sweeteners: study of the thermal degradation kinetic

The purpose of this study was the development of formulations of white chocolate for diabetics with replacement of sucrose by sucralose (Su) and Stevia (St) using a mixture experimental design. The kinetic studies of thermodegradation which showed that binary combinations of Stevia with sucrose had synergistic effects since the matrix presented a lower thermal sensitivity to the non-enzymatic browning reaction than the samples formulated from the individual components. The phenomena of blooming during storage were studied by a computer vision system and image analysis. The results of sensory analysis revealed that the sample 100%St was not acceptable; however, combining Stevia with sucrose and sucralose, acceptable sensory chocolates were obtained with no statistically significant differences, compared to control 100%S (P> 0.05). Our study provides a chocolate suitable for diabetics, with an appropriate combination of sensorially acceptable sweeteners with higher stability than control sample.Fil: Rodriguez Furlan, Laura Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Invest. En Tecnología Química; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Baracco, Yanina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Zaritzky, Noemi. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Campderrós, Mercedes. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentin

Visual/infrared interferometry of Orion Trapezium stars: Preliminary dynamical orbit and aperture synthesis imaging of the Theta 1 Orionis C system

Located in the Orion Trapezium cluster, Theta 1 Orionis C is one of the youngest and nearest high-mass stars (O5-O7) and also known to be a close binary system. Using new multi-epoch visual and near-infrared bispectrum speckle interferometric observations obtained at the BTA 6 m telescope, and IOTA near-infrared long-baseline interferometry, we trace the orbital motion of the Theta 1 Ori C components over the interval 1997.8 to 2005.9, covering a significant arc of the orbit. Besides fitting the relative position and the flux ratio, we apply aperture synthesis techniques to our IOTA data to reconstruct a model-independent image of the Theta 1 Ori C binary system. The orbital solutions suggest a high eccentricity (e approx. 0.91) and short-period (P approx. 10.9 yrs) orbit. As the current astrometric data only allows rather weak constraints on the total dynamical mass, we present the two best-fit orbits. From these orbital solutions one can be favoured, implying a system mass of 48 M_sun and a distance to the Trapezium cluster of 434 pc. When also taking the measured flux ratio and the derived location in the HR-diagram into account, we find good agreement for all observables, assuming a spectral type of O5.5 for Theta 1 Ori C1 (M=34.0 M_sun) and O9.5 for C2 (M=15.5 M_sun). We find indications that the companion C2 is massive itself, which makes it likely that its contribution to the intense UV radiation field of the Trapezium cluster is non-negligible. Furthermore, the high eccentricity of the preliminary orbit solution predicts a very small physical separation during periastron passage (approx. 1.5 AU, next passage around 2007.5), suggesting strong wind-wind interaction between the two O stars.Comment: 13 pages, 9 figures, Accepted for publication in Astronomy & Astrophysic

Quantifying and containing the curse of high resolution coronal imaging

Future missions such as Solar Orbiter (SO), InterHelioprobe, or Solar Probe aim at approaching the Sun closer than ever before, with on board some high resolution imagers (HRI) having a subsecond cadence and a pixel area of about $(80km)^2$ at the Sun during perihelion. In order to guarantee their scientific success, it is necessary to evaluate if the photon counts available at these resolution and cadence will provide a sufficient signal-to-noise ratio (SNR). We perform a first step in this direction by analyzing and characterizing the spatial intermittency of Quiet Sun images thanks to a multifractal analysis. We identify the parameters that specify the scale-invariance behavior. This identification allows next to select a family of multifractal processes, namely the Compound Poisson Cascades, that can synthesize artificial images having some of the scale-invariance properties observed on the recorded images. The prevalence of self-similarity in Quiet Sun coronal images makes it relevant to study the ratio between the SNR present at SoHO/EIT images and in coarsened images. SoHO/EIT images thus play the role of 'high resolution' images, whereas the 'low-resolution' coarsened images are rebinned so as to simulate a smaller angular resolution and/or a larger distance to the Sun. For a fixed difference in angular resolution and in Spacecraft-Sun distance, we determine the proportion of pixels having a SNR preserved at high resolution given a particular increase in effective area. If scale-invariance continues to prevail at smaller scales, the conclusion reached with SoHO/EIT images can be transposed to the situation where the resolution is increased from SoHO/EIT to SO/HRI resolution at perihelion.Comment: 25 pages, 1 table, 7 figure

Imaging starspot evolution on Kepler target KIC 5110407 using light curve inversion

The Kepler target KIC 5110407, a K-type star, shows strong quasi-periodic light curve fluctuations likely arising from the formation and decay of spots on the stellar surface rotating with a period of 3.4693 days. Using an established light-curve inversion algorithm, we study the evolution of the surface features based on Kepler space telescope light curves over a period of two years (with a gap of .25 years). At virtually all epochs, we detect at least one large spot group on the surface causing a 1-10% flux modulation in the Kepler passband. By identifying and tracking spot groups over a range of inferred latitudes, we measured the surface differential rotation to be much smaller than that found for the Sun. We also searched for a correlation between the seventeen stellar flares that occurred during our observations and the orientation of the dominant surface spot at the time of each flare. No statistically-significant correlation was found except perhaps for the very brightest flares, suggesting most flares are associated with regions devoid of spots or spots too small to be clearly discerned using our reconstruction technique. While we may see hints of long-term changes in the spot characteristics and flare statistics within our current dataset, a longer baseline of observation will be needed to detect the existence of a magnetic cycle in KIC 5110407.Comment: 32 pages, 15 figures, accepted to Ap

Unified functional network and nonlinear time series analysis for complex systems science: The pyunicorn package

We introduce the \texttt{pyunicorn} (Pythonic unified complex network and recurrence analysis toolbox) open source software package for applying and combining modern methods of data analysis and modeling from complex network theory and nonlinear time series analysis. \texttt{pyunicorn} is a fully object-oriented and easily parallelizable package written in the language Python. It allows for the construction of functional networks such as climate networks in climatology or functional brain networks in neuroscience representing the structure of statistical interrelationships in large data sets of time series and, subsequently, investigating this structure using advanced methods of complex network theory such as measures and models for spatial networks, networks of interacting networks, node-weighted statistics or network surrogates. Additionally, \texttt{pyunicorn} provides insights into the nonlinear dynamics of complex systems as recorded in uni- and multivariate time series from a non-traditional perspective by means of recurrence quantification analysis (RQA), recurrence networks, visibility graphs and construction of surrogate time series. The range of possible applications of the library is outlined, drawing on several examples mainly from the field of climatology.Comment: 28 pages, 17 figure