Towards Colour Imaging with the Image Ranger

Many of the colour images captured by different types of digital camera do not provide quality colour image according to human visual perception. In this study we explore technique for colour correction of the colour images from the Waikato Image Ranger. Colour images were captured using three different illuminants with the Waikato Image Ranger. The colour image formed from the ranger data do not have good quality because illuminants used do not match usual RGB standard illuminants. The spectral power distribution values of the illuminants were measured using spectroradiometer. To calculate tristimulus values the reflectance function of the scene is required. A mechanism of calculating the reflectance functions from the ranger data using genetic algorithm was explained. The reflectance functions are approximated using variable Gaussian basis functions, and fit to the ranger colour triplets by genetic algorithms. From the estimated reflectance functions standard CRT RGB values were calculated. It was found that the genetic algorithm approach was for too slow for practical purposes and produced images with far too much colour variation

Neonatal Infrared Thermography Image Processing

Tesina feta en col.laboració amb RWTH AachenThe temperature changes inside incubator a ect the newborns, who are the most delicate patients. The project proposes an innovative method to monitor the skin temperature of the neonates. The temperature monitoring is carried out by a virtual sensor. This virtual sensor is based in an infrared thermal camera that is placed outside the incubator. In order to obtain the infrared radiation through the incubator Plexiglas, an infrared transparent window is required. The experiment carried out was focused on obtaining the transparent properties in the infrared spectral range measurement of this window. On the other hand, the thermal imaging processing is necessary to obtain the thermal information from the infrared imager and to be able to track the region of interest throughout the eld of view

Bio-Inspired Multi-Spectral Imaging Sensors and Algorithms for Image Guided Surgery

Image guided surgery (IGS) utilizes emerging imaging technologies to provide additional structural and functional information to the physician in clinical settings. This additional visual information can help physicians delineate cancerous tissue during resection as well as avoid damage to near-by healthy tissue. Near-infrared (NIR) fluorescence imaging (700 nm to 900 nm wavelengths) is a promising imaging modality for IGS, namely for the following reasons: First, tissue absorption and scattering in the NIR window is very low, which allows for deeper imaging and localization of tumor tissue in the range of several millimeters to a centimeter depending on the tissue surrounding the tumor. Second, spontaneous tissue fluorescence emission is minimal in the NIR region, allowing for high signal-to-background ratio imaging compared to visible spectrum fluorescence imaging. Third, decoupling the fluorescence signal from the visible spectrum allows for optimization of NIR fluorescence while attaining high quality color images. Fourth, there are two FDA approved fluorescent dyes in the NIR region—namely methylene blue (MB) and indocyanine green—which can help to identify tumor tissue due to passive accumulation in human subjects. The aforementioned advantages have led to the development of NIR fluorescence imaging systems for a variety of clinical applications, such as sentinel lymph node imaging, angiography, and tumor margin assessment. With these technological advances, secondary surgeries due to positive tumor margins or damage to healthy organs can be largely mitigated, reducing the emotional and financial toll on the patient. Currently, several NIR fluorescence imaging systems (NFIS) are available commercially or are undergoing clinical trials, such as FLARE, SPY, PDE, Fluobeam, and others. These systems capture multi-spectral images using complex optical equipment and are combined with real-time image processing to present an augmented view to the surgeon. The information is presented on a standard monitor above the operating bed, which requires the physician to stop the surgical procedure and look up at the monitor. The break in the surgical flow sometimes outweighs the benefits of fluorescence based IGS, especially in time-critical surgical situations. Furthermore, these instruments tend to be very bulky and have a large foot print, which significantly complicates their adoption in an already crowded operating room. In this document, I present the development of a compact and wearable goggle system capable of real-time sensing of both NIR fluorescence and color information. The imaging system is inspired by the ommatidia of the monarch butterfly, in which pixelated spectral filters are integrated with light sensitive elements. The pixelated spectral filters are fabricated via a carefully optimized nanofabrication procedure and integrated with a CMOS imaging array. The entire imaging system has been optimized for high signal-to-background fluorescence imaging using an analytical approach, and the efficacy of the system has been experimentally verified. The bio-inspired spectral imaging sensor is integrated with an FPGA for compact and real-time signal processing and a wearable goggle for easy integration in the operating room. The complete imaging system is undergoing clinical trials at Washington University in the St. Louis Medical School for imaging sentinel lymph nodes in both breast cancer patients and melanoma patients

FPI Based Hyperspectral Imager for the Complex Surfaces—Calibration, Illumination and Applications

Hyperspectral imaging (HSI) applications for biomedical imaging and dermatological applications have been recently under research interest. Medical HSI applications are non-invasive methods with high spatial and spectral resolution. HS imaging can be used to delineate malignant tumours, detect invasions, and classify lesion types. Typical challenges of these applications relate to complex skin surfaces, leaving some skin areas unreachable. In this study, we introduce a novel spectral imaging concept and conduct a clinical pre-test, the findings of which can be used to develop the concept towards a clinical application. The SICSURFIS spectral imager concept combines a piezo-actuated Fabry–Pérot interferometer (FPI) based hyperspectral imager, a specially designed LED module and several sizes of stray light protection cones for reaching and adapting to the complex skin surfaces. The imager is designed for the needs of photometric stereo imaging for providing the skin surface models (3D) for each captured wavelength. The captured HS images contained 33 selected wavelengths (ranging from 477 nm to 891 nm), which were captured simultaneously with accordingly selected LEDs and three specific angles of light. The pre-test results show that the data collected with the new SICSURFIS imager enable the use of the spectral and spatial domains with surface model information. The imager can reach complex skin surfaces. Healthy skin, basal cell carcinomas and intradermal nevi lesions were classified and delineated pixel-wise with promising results, but further studies are needed. The results were obtained with a convolutional neural network

FPI Based Hyperspectral Imager for the Complex Surfaces—Calibration, Illumination and Applications

Hyperspectral imaging (HSI) applications for biomedical imaging and dermatological applications have been recently under research interest. Medical HSI applications are non-invasive methods with high spatial and spectral resolution. HS imaging can be used to delineate malignant tumours, detect invasions, and classify lesion types. Typical challenges of these applications relate to complex skin surfaces, leaving some skin areas unreachable. In this study, we introduce a novel spectral imaging concept and conduct a clinical pre-test, the findings of which can be used to develop the concept towards a clinical application. The SICSURFIS spectral imager concept combines a piezo-actuated Fabry–Pérot interferometer (FPI) based hyperspectral imager, a specially designed LED module and several sizes of stray light protection cones for reaching and adapting to the complex skin surfaces. The imager is designed for the needs of photometric stereo imaging for providing the skin surface models (3D) for each captured wavelength. The captured HS images contained 33 selected wavelengths (ranging from 477 nm to 891 nm), which were captured simultaneously with accordingly selected LEDs and three specific angles of light. The pre-test results show that the data collected with the new SICSURFIS imager enable the use of the spectral and spatial domains with surface model information. The imager can reach complex skin surfaces. Healthy skin, basal cell carcinomas and intradermal nevi lesions were classified and delineated pixel-wise with promising results, but further studies are needed. The results were obtained with a convolutional neural network

In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography

We present in vivo volumetric images of human retinal micro-circulation using Fourier-domain optical coherence tomography (Fd-OCT) with the phase-variance based motion contrast method. Currently fundus fluorescein angiography (FA) is the standard technique in clinical settings for visualizing blood circulation of the retina. High contrast imaging of retinal vasculature is achieved by injection of a fluorescein dye into the systemic circulation. We previously reported phase-variance optical coherence tomography (pvOCT) as an alternative and non-invasive technique to image human retinal capillaries. In contrast to FA, pvOCT allows not only noninvasive visualization of a two-dimensional retinal perfusion map but also volumetric morphology of retinal microvasculature with high sensitivity. In this paper we report high-speed acquisition at 125 kHz A-scans with pvOCT to reduce motion artifacts and increase the scanning area when compared with previous reports. Two scanning schemes with different sampling densities and scanning areas are evaluated to find optimal parameters for high acquisition speed in vivo imaging. In order to evaluate this technique, we compare pvOCT capillary imaging at 3x3 mm^2 and 1.5x1.5 mm^2 with fundus FA for a normal human subject. Additionally, a volumetric view of retinal capillaries and a stitched image acquired with ten 3x3 mm^2 pvOCT sub-volumes are presented. Visualization of retinal vasculature with pvOCT has potential for diagnosis of retinal vascular diseases

Integration, Testing, And Analysis Of Multispectral Imager On Small Unmanned Aerial System For Skin Detection

Small Unmanned Aerial Systems (SUAS) have been utilized by the military, geological researchers, and first responders, to provide information about the environment in real time. Hyperspectral Imagery (HSI) provides high resolution data in the spatial and spectral dimension; all objects, including skin have unique spectral signatures. However, little research has been done to integrate HSI into SUAS due to their cost and form factor. Multispectral Imagery (MSI) has proven capable of dismount detection with several distinct wavelengths. This research proposes a spectral imaging system that can detect dismounts on SUAS. Also, factors that pertain to accurate dismount detection with an SUAS are explored. Dismount skin detection from an aerial platform also has an inherent difficulty compared to ground-based platforms. Computer vision registration, stereo camera calibration, and geolocation from autopilot telemetry are utilized to design a dismount detection platform with the Systems Engineering methodology. An average 5.112% difference in ROC AUC values that compared a line scan spectral imager to the prototype area scan imager was recorded. Results indicated that an SUAS-based Spectral Imagers are capable tools in dismount detection protocols. Deficiencies associated with the test expedient prototype are discussed and recommendations for further improvements are provided

Analysis of image noise in multispectral color acquisition

The design of a system for multispectral image capture will be influenced by the imaging application, such as image archiving, vision research, illuminant modification or improved (trichromatic) color reproduction. A key aspect of the system performance is the effect of noise, or error, when acquiring multiple color image records and processing of the data. This research provides an analysis that allows the prediction of the image-noise characteristics of systems for the capture of multispectral images. The effects of both detector noise and image processing quantization on the color information are considered, as is the correlation between the errors in the component signals. The above multivariate error-propagation analysis is then applied to an actual prototype system. Sources of image noise in both digital camera and image processing are related to colorimetric errors. Recommendations for detector characteristics and image processing for future systems are then discussed