Close-range hyperspectral spectroscopy reveals leaf water content dynamics

Water plays a crucial role in maintaining plant functionality and drives many ecophysiological processes. The distribution of water resources is in a continuous change due to global warming affecting the productivity of ecosystems around the globe, but there is a lack of non-destructive methods capable of continuous monitoring of plant and leaf water content that would help us in understanding the consequences of the redistribution of water. We studied the utilization of novel small hyperspectral sensors in the 1350-1650 nm and 2000-2450 nm spectral ranges in non-destructive estimation of leaf water content in laboratory and field conditions. We found that the sensors captured up to 96% of the variation in equivalent water thickness (EWT, g/m(2)) and up to 90% of the variation in relative water content (RWC). Further tests were done with an indoor plant (Dracaena marginate Lem.) by continuously measuring leaf spectra while drought conditions developed, which revealed detailed diurnal dynamics of leaf water content. The laboratory findings were supported by field measurements, where repeated leaf spectra measurements were in fair agreement (R-2 = 0.70) with RWC and showed similar diurnal dynamics. The estimation of leaf mass per area (LMA) using leaf spectra was investigated as a pathway to improved RWC estimation, but no significant improvement was found. We conclude that close-range hyper spectral spectroscopy can provide a novel tool for continuous measurement of leaf water content at the single leaf level and help us to better understand plant responses to varying environmental conditions.Peer reviewe

Terrestrial laser scanning intensity captures diurnal variation in leaf water potential

During the past decades, extreme events have become more prevalent and last longer, and as a result drought-induced plant mortality has increased globally. Timely information on plant water dynamics is essential for understanding and anticipating drought-induced plant mortality. Leaf water potential (ΨL), which is usually measured destructively, is the most common metric that has been used for decades for measuring water stress. Remote sensing methods have been developed to obtain information on water dynamics from trees and forested landscapes. However, the spatial and temporal resolutions of the existing methods have limited our understanding of the water dynamics and diurnal variation of ΨL within single trees. Thus, we investigated the capability of terrestrial laser scanning (TLS) intensity in observing diurnal variation in ΨL during a 50-h monitoring period. We aimed to improve the understanding on how large a part of the diurnal variation in ΨL can be captured using TLS intensity observations. We found that TLS intensity at the 905 nm wavelength measured from a static position was able to explain 77% of the variation in ΨL for three trees of two tree species with a root mean square error of 0.141 MPa. Based on our experiment with three trees, a time series of TLS intensity measurements can be used in detecting changes in ΨL, and thus it is worthwhile to expand the investigations to cover a wider range of tree species and forests and further increase our understanding of plant water dynamics at wider spatial and temporal scales

Four Dimensional Mapping of Vegetation Moisture Content Using Dual-Wavelength Terrestrial Laser Scanning

Recently, terrestrial laser scanning (TLS) has shown potential in measuring vegetation biochemical traits in three dimensions (3D) by using reflectance derived from backscattered intensity data. The 3D estimates can provide information about the vertical heterogeneity of canopy biochemical traits which affects canopy reflectance but cannot be measured from spaceborne and airborne optical remote sensing data. Leaf equivalent water thickness (EWT), a metric widely used in vegetation health monitoring, has been successfully linked to the normalized difference index (NDI) of near and shortwave infrared wavelengths at the leaf level. However, only two previous studies have linked EWT to NDI at the canopy level in field campaigns. In this study, an NDI consisting of 808 and 1550 nm wavelengths was used to generate 3D EWT estimates at the canopy level in a broadleaf mixed-species tree plot during and after a heatwave. The relative error in EWT estimates was 6% across four different species. Temporal changes in EWT were measured, and the accuracy varied between trees, a factor of the errors in EWT estimates on both dates. Vertical profiles of EWT were generated for six trees and showed vertical heterogeneity and variation between species. The change in EWT vertical profiles during and after the heatwave differed between trees, demonstrating that trees reacted in different ways to the drought condition

Three dimensional estimation of vegetation moisture content using dual-wavelength terrestrial laser scanning

PhD ThesisLeaf Equivalent Water Thickness (EWT) is a water status metric widely used in vegetation health monitoring. Optical Remote Sensing (RS) data, spaceborne and airborne, can be used to estimate canopy EWT at landscape level, but cannot provide information about EWT vertical heterogeneity, or estimate EWT predawn. Dual-wavelength Terrestrial Laser Scanning (TLS) can overcome these limitations, as TLS intensity data, following radiometric corrections, can be used to estimate EWT in three dimensions (3D). In this study, a Normalized Difference Index (NDI) of 808 nm wavelength, utilized in the Leica P20 TLS instrument, and 1550 nm wavelength, employed in the Leica P40 and P50 TLS systems, was used to produce 3D EWT estimates at canopy level. Intensity correction models were developed, and NDI was found to be able to minimize the incidence angle and leaf internal structure effects. Multiple data collection campaigns were carried out. An indoors dry-down experiment revealed a strong correlation between NDI and EWT at leaf level. At canopy level, 3D EWT estimates were generated with a relative error of 3 %. The method was transferred to a mixed-species broadleaf forest plot and 3D EWT estimates were generated with relative errors < 7 % across four different species. Next, EWT was estimated in six short-rotation willow plots during leaf senescence with relative errors < 8 %. Furthermore, a broadleaf mixed-species urban tree plot was scanned during and two months after a heatwave, and EWT temporal changes were successfully detected. Relative error in EWT estimates was 6 % across four tree species. The last step in this research was to study the effects of EWT vertical heterogeneity on forest plot reflectance. Two virtual forest plots were reconstructed in the Discrete Anisotropic Radiative Transfer (DART) model. 3D EWT estimates from TLS were utilized in the model and Sentinel-2A bands were simulated. The simulations revealed that the top four to five metres of canopy dominated the plot reflectance. The satellite sensor was not able to detect severe water stress that started in the lower canopy layers. This study showed the potential of using dual-wavelength TLS to provide important insights into the EWT distribution within the canopy, by mapping the EWT at canopy level in 3D. EWT was found to vary vertically within the canopy, with EWT and Leaf Mass per Area (LMA) being highly correlated, suggesting that sun leaves were able to hold more moisture than shade leaves. The EWT vertical profiles varied between species, and trees reacted in different ways during drought conditions, losing moisture from different canopy layers. The proposed method can provide time series of the change in EWT at very high spatial and temporal resolutions, as TLS instruments are active sensors, independent of the solar illumination. It also has the potential to provide EWT estimates at the landscape level, if coupled with automatic tree ii segmentation and leaf-wood separation techniques, and thus filling the gaps in the time series produced from satellite data. In addition, the technique can potentially allow the characterisation of whole-tree leaf water status and total water content, by combining the EWT estimates with Leaf Area Index (LAI) measurements, providing new insights into forest health and tree physiology.Egyptian Ministry of Higher Educatio