Epizoic acoelomorph flatworms compete with their coral host for zooplankton

Satisfying nutrient requirement of corals is still a major constraint for maintaining corals in marine aquariums. Corals are polytrophic in nature. Heterotrophic feeding on zooplankton is one of the corals’ strategies to overcome nutrient deficiency. Artemia salina nauplii are commonly used as biocarriers for many fish larvae in aquaculture and can also serve as a biocarrier for coral in aquariums, provided coral acceptability, optimal feeding rate, and digestibility of the nauplii are well understood. Feeding rate and digestibility of coral fed on A. salina nauplii at 100, 2,000, 4,000, 6,000, and 10,000 ind. l-1 under light and dark conditions was assessed in this study. The maximum feeding rates of Galaxea fascicularis under light and dark conditions was 113.6 ind. polyp-1 h-1 and 76.9 ind. polyp-1 h-1, respectively. The daily feeding rates of G. fascicularis varies and depends on nauplii density. Light plays an important role in coral feeding. Nevertheless, the quantity of A. salina nauplii consumed by the coral under light and dark conditions was not significantly different (P > 0.05). A. salina nauplii are well accepted by G. fascicularis. Complete nauplii digestion was observed after 180 min. Digestibility of A. salina nauplii by G. fascicularis was positively correlated with digestion time

DESKRIPSI AKTIVITAS HARIAN MONYET EKOR PANJANG (MACACA FASCICULARIS) DI KAWASAN SEUNAPIT SEULAWAH KABUPATEN ACEH BESAR

Kata Kunci: Aktivitas harian, Macaca fascicularis, Kawasan Seunapit, SeulawahPenelitian mengenai deskripsi aktivitas harian monyet ekor panjang (Macaca fascicularis) telah dilakukan di kawasan Seulawah Kabupaten Aceh Besar dengan tujuan untuk mengetahui waktu aktivitas harian Macaca fascicularis. Penelitian dilaksanakan pada bulan April 2015 sampai dengan Mei 2015 pada 3 kriteria Macaca fascicularis yaitu Macaca fascicularis jantan dewasa, Macaca fascicularis betina dewasa dan Macaca fascicularis juvenile. Aktivitas harian dibedakan menjadi 7 kategori yaitu makan (feeding), istirahat (resting), berkelahi (fighting), reproduksi (mating), berpindah (moving), bersuara (vocalization) dan menunggu (food waiting). Data aktivitas harian diambil dengan menggunakan metode direct observation dengan teknik scan sampling dan focal animal sampling dengan interval 10 menit mulai pukul 07.00 hingga pukul 18.00 dan dianalisis dengan menggunakan ANOVA. Hasil penelitian menunjukkan bahwa perilaku makan 27%, istirahat 28%, berkelahi 4%, reproduksi 0,4%, berpindah 37%, menunggu 3% dan bersuara 2%

Long-Tailed Macaques (Macaca fascicularis) and Humans Interactions in Grojogan Sewu Natural Park (TWA GS), Karanganyar Regency, Central Java Province

Macaca fascicularis is one species of primate that easily adapts to various habitats, such as disturbances habitat, like a natural park. The conflict between M. fascicularis and human increases due to changes in ecology. In Borneo, Sumatra, Malaysia, Mauritus, and Thailand, M. fascicularis has become pests because destroyed orchards and plantations. Interaction between M. fascicularis and human, which occurred at several natural parks in Singapura and Kaliurang caused by M. fascicularis’s attraction to food brought by humans. This research aimed to study the interaction between M. fascicularis with the humans in Grojogan Sewu Natural Park (TWA GS) by direct observation and also an interview with visitors, workers, and villagers. Based on direct observation, frequency of affiliation interaction between humans and M. fascicularis (55.56%) is higher than agonistic interaction (44.4%). The dominant affiliation interaction was sitting nearby the visitors (42.96%); meanwhile, the most dominant agonistic interaction was stealing (54.12%) because the M. fascicularis attracted to food (67.02%). Workers reported having more nuisance problem with M. fascicularis than visitors and villagers. Almost all of the respondents thought that M. fascicularis needs to be kept alive in the park, and consider conservation and protection of the macaques are essential

Comparison of Blood and Brain Mercury Levels in Infant Monkeys Exposed to Methylmercury or Vaccines Containing Thimerosal

Thimerosal is a preservative that has been used in manufacturing vaccines since the 1930s. Reports have indicated that infants can receive ethylmercury (in the form of thimerosal) at or above the U.S. Environmental Protection Agency guidelines for methylmercury exposure, depending on the exact vaccinations, schedule, and size of the infant. In this study we compared the systemic disposition and brain distribution of total and inorganic mercury in infant monkeys after thimerosal exposure with those exposed to MeHg. Monkeys were exposed to MeHg (via oral gavage) or vaccines containing thimerosal (via intramuscular injection) at birth and 1, 2, and 3 weeks of age. Total blood Hg levels were determined 2, 4, and 7 days after each exposure. Total and inorganic brain Hg levels were assessed 2, 4, 7, or 28 days after the last exposure. The initial and terminal half-life of Hg in blood after thimerosal exposure was 2.1 and 8.6 days, respectively, which are significantly shorter than the elimination half-life of Hg after MeHg exposure at 21.5 days. Brain concentrations of total Hg were significantly lower by approximately 3-fold for the thimerosal-exposed monkeys when compared with the MeHg infants, whereas the average brain-to-blood concentration ratio was slightly higher for the thimerosal-exposed monkeys (3.5 ± 0.5 vs. 2.5 ± 0.3). A higher percentage of the total Hg in the brain was in the form of inorganic Hg for the thimerosal-exposed monkeys (34% vs. 7%). The results indicate that MeHg is not a suitable reference for risk assessment from exposure to thimerosal-derived Hg. Knowledge of the toxicokinetics and developmental toxicity of thimerosal is needed to afford a meaningful assessment of the developmental effects of thimerosal-containing vaccines

POPULASI MONYET EKOR PANJANG (Macaca fascicularis) DI KAWASAN PESISIR PANTAI NIPAH PULO ACEH

Macaca fascicularis merupakan salah satu spesies dari kelas Mammalia dengan tingkat populasi yang banyak. Kemampuan adaptasi yang tinggi berhasil membuat populasi Macaca fascicularis sangat luas persebarannya. Penelitian ini dilakukan dengan metode Line Transect. Data dianalisis dalam bentuk tabulasi. Penelitian ini bertujuan mengetahui populasi monyet ekor panjang (Macaca fascicularis) di kawasan pesisir pantai Nipah Pulo Aceh. Populasi monyet ekor panjang (Macaca fascicularis) di kawasan pesisir pantai Nipah terdiri dari 16 individu dengan struktur populasi 4 jantan dewasa, 4 betina dewasa, 5 muda, dan 3 anakan. Analisis data menggunakan rumus kepadatan populasi. Kepadatan populasi yang didapatkan yaitu 8 indiv/Km2. Monyet ekor panjang (Macaca fascicularis) merupakan hewan diurnal

Long-Tailed Macaques (Macaca fascicularis) and Humans Interactions in Grojogan Sewu Natural Park (TWA GS), Karanganyar Regency, Central Java Province

Macaca fascicularis is one species of primate that easily adapts to various habitats, such as disturbances habitat, like a natural park. The conflict between M. fascicularis and human increases due to changes in ecology. In Borneo, Sumatra, Malaysia, Mauritus, and Thailand, M. fascicularis has become pests because destroyed orchards and plantations. Interaction between M. fascicularis and human, which occurred at several natural parks in Singapura and Kaliurang caused by M. fascicularis’s attraction to food brought by humans. This research aimed to study the interaction between M. fascicularis with the humans in Grojogan Sewu Natural Park (TWA GS) by direct observation and also an interview with visitors, workers, and villagers. Based on direct observation, frequency of affiliation interaction between humans and M. fascicularis (55.56%) is higher than agonistic interaction (44.4%). The dominant affiliation interaction was sitting nearby the visitors (42.96%); meanwhile, the most dominant agonistic interaction was stealing (54.12%) because the M. fascicularis attracted to food (67.02%). Workers reported having more nuisance problem with M. fascicularis than visitors and villagers. Almost all of the respondents thought that M. fascicularis needs to be kept alive in the park, and consider conservation and protection of the macaques are essential

Determination of prey capture rates in the stony coral Galaxea fascicularis: a critical reconsideration of the clearance rate concept

In order to determine optimal feeding regimes for captive corals, prey capture by the scleractinian coral Galaxea fascicularis was determined by measuring clearance of prey items from the surrounding water. Colonies of G. fascicularis (sized between 200 and 400 polyps) were incubated in 1300 ml incubation chambers. Nauplii of the brine shrimp Artemia sp. were used as the prey item. A series of incubation experiments was conducted to determine the maximal capture per feeding event and per day. To determine maximal capture per feeding event, total uptake of nauplii after one hour was determined for different prey item availabilities ranging from 50 to 4000 nauplii per polyp. To determine maximal capture per day, the corals were subjected to four repetitive feeding events at three different prey item densities (50, 100 and 150 nauplii per polyp). Alongside these quantitative experiments, it was tested to what extent the feeding response of corals is triggered by chemical cues. One hour after food addition, extract of Artemia nauplii was added to the incubation chambers to test its effect on subsequent prey capture rates. In all experiments, prey capture was expressed as the number of nauplii consumed per coral polyp. Total capture of Artemia nauplii by G. fascicularis after a single feeding event increased linearly up till a prey item availability of 2000 nauplii per polyp. Maximal capture per feeding event was estimated at 1200 nauplii per polyp, which is higher than rates reported in previous studies. It became apparent that at high densities of Artemia nauplii, the clearance rate method does not discriminate between active capture and passive sedimentation. Repetitive feeding with 50 nauplii per polyp resulted in a constant total prey capture per feeding event. At a supply of 100 nauplii per polyp, total capture decreased after the first feeding event, and remained constant during the subsequent feeding events at a level comparable to the lower food availability. However, at a supply of 150 nauplii per polyp, total capture per event was higher throughout the entire four-hour incubation period, which obfuscates an accurate estimation of the maximal daily food uptake. In all incubations, a decrease in capture efficiency was observed within the course of the feeding event. In all repetitive feeding experiments, capture efficiency increased immediately upon addition of a new batch of food. This increase in efficiency was not caused by a priming effect of extract of Artemia. The inconsistencies in the data show that estimates of prey capture based on clearance rates should be interpreted with caution, because this method does not take into account potential dynamics of prey capture and release

Absorbed radiation dosimetry of the D3-specific PET radioligand [18F]FluorTriopride estimated using rodent and nonhuman primate

[(18)F]FluorTriopride ([(18)F]FTP) is a dopamine D(3)-receptor preferring radioligand with potential for investigation of neuropsychiatric disorders including Parkinson disease, dystonia and schizophrenia. Here we estimate human radiation dosimetry for [(18)F]FTP based on the ex-vivo biodistribution in rodents and in vivo distribution in nonhuman primates. Biodistribution data were generated using male and female Sprague-Dawley rats injected with ~370 KBq of [(18)F]FTP and euthanized at 5, 30, 60, 120, and 240 min. Organs of interest were dissected, weighed and assayed for radioactivity content. PET imaging studies were performed in two male and one female macaque fascicularis administered 143-190 MBq of [(18)F]FTP and scanned whole-body in sequential sections. Organ residence times were calculated based on organ time activity curves (TAC) created from regions of Interest. OLINDA/EXM 1.1 was used to estimate human radiation dosimetry based on scaled organ residence times. In the rodent, the highest absorbed radiation dose was the upper large intestines (0.32-0.49 mGy/MBq), with an effective dose of 0.07 mSv/MBq in males and 0.1 mSv/MBq in females. For the nonhuman primate, however, the gallbladder wall was the critical organ (1.81 mGy/MBq), and the effective dose was 0.02 mSv/MBq. The species discrepancy in dosimetry estimates for [(18)F]FTP based on rat and primate data can be attributed to the slower transit of tracer through the hepatobiliary track of the primate compared to the rat, which lacks a gallbladder. Out findings demonstrate that the nonhuman primate model is more appropriate model for estimating human absorbed radiation dosimetry when hepatobiliary excretion plays a major role in radiotracer elimination

Distribution and prevalence of malaria parasites among long-tailed macaques (Macaca fascicularis) in regional populations across Southeast Asia.

BackgroundPlasmodium knowlesi and Plasmodium cynomolgi are two malaria parasites naturally transmissible between humans and wild macaque through mosquito vectors, while Plasmodium inui can be experimentally transmitted from macaques to humans. One of their major natural hosts, the long-tailed macaque (Macaca fascicularis), is host to two other species of Plasmodium (Plasmodium fieldi and Plasmodium coatneyi) and is widely distributed in Southeast Asia. This study aims to determine the distribution of wild macaques infected with malarial parasites by examining samples derived from seven populations in five countries across Southeast Asia.MethodsPlasmodium knowlesi, P. cynomolgi, P. coatneyi, P. inui and P. fieldi, were detected using nested PCR assays in DNA samples from 276 wild-caught long-tailed macaques. These samples had been derived from macaques captured at seven locations, two each in the Philippines (n = 68) and Indonesia (n = 70), and one each in Cambodia (n = 54), Singapore (n = 40) and Laos (n = 44). The results were compared with previous studies of malaria parasites in long-tailed macaques from other locations in Southeast Asia. Fisher exact test and Chi square test were used to examine the geographic bias of the distribution of Plasmodium species in the macaque populations.ResultsOut of 276 samples tested, 177 were Plasmodium-positive, with P. cynomolgi being the most common and widely distributed among all long-tailed macaque populations (53.3 %) and occurring in all populations examined, followed by P. coatneyi (20.4 %), P. inui (12.3 %), P. fieldi (3.4 %) and P. knowlesi (0.4 %). One P. knowlesi infection was detected in a macaque from Laos, representing the first documented case of P. knowlesi in wildlife in Laos. Chi square test showed three of the five parasites (P. knowlesi, P. coatneyi, P. cynomolgi) with significant bias in prevalence towards macaques from Malaysian Borneo, Cambodia, and Southern Sumatra, respectively.ConclusionsThe prevalence of malaria parasites, including those that are transmissible to humans, varied among all sampled regional populations of long-tailed macaques in Southeast Asia. The new discovery of P. knowlesi infection in Laos, and the high prevalence of P. cynomolgi infections in wild macaques in general, indicate the strong need of public advocacy in related countries

Conformational adaptation of Asian macaque TRIMCyp directs lineage specific antiviral activity

TRIMCyps are anti-retroviral proteins that have arisen independently in New World and Old World primates. All TRIMCyps comprise a CypA domain fused to the tripartite domains of TRIM5α but they have distinct lentiviral specificities, conferring HIV-1 restriction in New World owl monkeys and HIV-2 restriction in Old World rhesus macaques. Here we provide evidence that Asian macaque TRIMCyps have acquired changes that switch restriction specificity between different lentiviral lineages, resulting in species-specific alleles that target different viruses. Structural, thermodynamic and viral restriction analysis suggests that a single mutation in the Cyp domain, R69H, occurred early in macaque TRIMCyp evolution, expanding restriction specificity to the lentiviral lineages found in African green monkeys, sooty mangabeys and chimpanzees. Subsequent mutations have enhanced restriction to particular viruses but at the cost of broad specificity. We reveal how specificity is altered by a scaffold mutation, E143K, that modifies surface electrostatics and propagates conformational changes into the active site. Our results suggest that lentiviruses may have been important pathogens in Asian macaques despite the fact that there are no reported lentiviral infections in current macaque populations