24,735 research outputs found

    In Pursuit of Experience: The Authentic Documentation of Experience in Beat Generation Literature

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    Throughout their lives the authors of The Beat Generation sought an escape from the conformity of mid-century American life, in favour of fresh thrilling experiences to influence their writing. The writers of the Beat Generation developed writing methods that authentically document their real-life experiences. Therefore, this thesis examines the documentary nature of literature that came out of this Generation. The first section of the essay explores Beat literature as memoir; arguing that Kerouac's prose is based on his own first-hand experience recollected after the event. This section also argues that due to its fast pace and lack of revision, the Spontaneous Prose Method can be used by authors as a form suited to the authentic documentation of experience. The second chapter looks at the use of transcription methods to document a moment, or specific event, written during the experience. This chapter compares Gary Snyder's Riprap and Cold Mountain Poems, Ginsberg's 'Wichita Vortex Sutra', and Kerouac's Blues Poems as poetry that authentically portrays a moment of experience to the reader. The final chapter explores the more experimental methods of documentation, and whether any authenticity was lost to experimentation. The chapter also explores the Beat use of drugs on the content and form of the literature

    Dimension-8 SMEFT Analysis of Minimal Scalar Field Extensions of the Standard Model

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    We analyze the constraints obtainable from present data using the Standard Model Effective Field Theory (SMEFT) on extensions of the Standard Model with additional electroweak singlet or triplet scalar fields. We compare results obtained using only contributions that are linear in dimension-6 operator coefficients with those obtained including terms quadratic in these coefficients as well as contributions that are linear in dimension-8 operator coefficients. We also implement theoretical constraints arising from the stability of the electroweak vacuum and perturbative unitarity. Analyzing the models at the dimension-8 level constrains scalar couplings that are not bounded at the dimension-6 level. The strongest experimental constraints on the singlet model are provided by Higgs coupling measurements, whereas electroweak precision observables provide the strongest constraints on the triplet model. In the singlet model the present di-Higgs constraints already play a significant role. We find that the current constraints on model parameters are already competitive with those anticipated from future di- and tri-Higgs measurements. We compare our results with calculations in the full model, exhibiting the improvements when higher-order SMEFT terms are included. We also identify regions in parameter space where the SMEFT approximation appears to break down. We find that the combination of current constraints with the theoretical bounds still admits regions where the SMEFT approach is not valid, particularly for lower scalar boson masses.Comment: 66 Pages, 14 Figures, 4 Table

    The enterovirus genome can be translated in an IRES-independent manner that requires the initiation factors eIF2A/eIF2D

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    RNA recombination in positive-strand RNA viruses is a molecular-genetic process, which permits the greatest evolution of the genome and may be essential to stabilizing the genome from the deleterious consequences of accumulated mutations. Enteroviruses represent a useful system to elucidate the details of this process. On the biochemical level, it is known that RNA recombination is catalyzed by the viral RNA-dependent RNA polymerase using a template-switching mechanism. For this mechanism to function in cells, the recombining genomes must be located in the same subcellular compartment. How a viral genome is trafficked to the site of genome replication and recombination, which is membrane associated and isolated from the cytoplasm, is not known. We hypothesized that genome translation was essential for colocalization of genomes for recombination. We show that complete inactivation of internal ribosome entry site (IRES)-mediated translation of a donor enteroviral genome enhanced recombination instead of impairing it. Recombination did not occur by a nonreplicative mechanism. Rather, sufficient translation of the nonstructural region of the genome occurred to support subsequent steps required for recombination. The noncanonical translation initiation factors, eIF2A and eIF2D, were required for IRES-independent translation. Our results support an eIF2A/eIF2D-dependent mechanism under conditions in which the eIF2-dependent mechanism is inactive. Detection of an IRES-independent mechanism for translation of the enterovirus genome provides an explanation for a variety of debated observations, including nonreplicative recombination and persistence of enteroviral RNA lacking an IRES. The existence of an eIF2A/eIF2D-dependent mechanism in enteroviruses predicts the existence of similar mechanisms in other viruses

    Fruit Crop Improvement with Genome Editing, In Vitro and Transgenic Approaches

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    Fruit species contribute to nutritional and health security by providing micronutrients, antioxidants, and bioactive phytoconstituents, and hence fruit-based products are becoming functional foods presently and for the future. Although conventional breeding methods have yielded improved varieties having fruit quality, aroma, antioxidants, yield, and nutritional traits, the threat of climate change and need for improvement in several other traits such as biotic and abiotic stress tolerance and higher nutritional quality has demanded complementary novel strategies. Biotechnological research in fruit crops has offered immense scope for large-scale multiplication of elite clones, in vitro, mutagenesis, and genetic transformation. Advanced molecular methods, such as genome-wide association studies (GWAS), QTLomics, genomic selection for the development of novel germplasm having functional traits for agronomic and nutritional quality, and enrichment of bioactive constituents through metabolic pathway engineering and development of novel products, are now paving the way for trait-based improvement for developing genetically superior varieties in fruit plant species for enhanced nutritional quality and agronomic performance. In this article, we highlight the applications of in vitro and molecular breeding approaches for use in fruit breeding

    Norsk rÄ kumelk, en kilde til zoonotiske patogener?

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    The worldwide emerging trend of eating “natural” foods, that has not been processed, also applies for beverages. According to Norwegian legislation, all milk must be pasteurized before commercial sale but drinking milk that has not been heat-treated, is gaining increasing popularity. Scientist are warning against this trend and highlights the risk of contracting disease from milkborne microorganisms. To examine potential risks associated with drinking unpasteurized milk in Norway, milk- and environmental samples were collected from dairy farms located in south-east of Norway. The samples were analyzed for the presence of specific zoonotic pathogens; Listeria monocytogenes, Campylobacter spp., and Shiga toxin-producing Escherichia coli (STEC). Cattle are known to be healthy carriers of these pathogens, and Campylobacter spp. and STEC have a low infectious dose, meaning that infection can be established by ingesting a low number of bacterial cells. L. monocytogenes causes one of the most severe foodborne zoonotic diseases, listeriosis, that has a high fatality rate. All three pathogens have caused milk borne disease outbreaks all over the world, also in Norway. During this work, we observed that the prevalence of the three examined bacteria were high in the environment at the examined farms. In addition, 7% of the milk filters were contaminated by STEC, 13% by L. monocytogenes and 4% by Campylobacter spp. Four of the STEC isolates detected were eaepositive, which is associated with the capability to cause severe human disease. One of the eae-positive STEC isolates were collected from a milk filter, which strongly indicate that Norwegian raw milk may contain potential pathogenic STEC. To further assess the possibilities of getting ill by STEC after consuming raw milk, we examined the growth of the four eae-positive STEC isolates in raw milk at different temperatures. All four isolates seemed to have ability to multiply in raw milk at 8°C, and one isolate had significant growth after 72 hours. Incubation at 6°C seemed to reduce the number of bacteria during the first 24 hours before cell death stopped. These findings highlight the importance of stable refrigerator temperatures, preferable < 4°C, for storage of raw milk. The L. monocytogenes isolates collected during this study show genetic similarities to isolates collected from urban and rural environmental locations, but different clones were predominant in agricultural environments compared to clinical and food environments. However, the results indicate that the same clone can persist in a farm over time, and that milk can be contaminated by L. monocytogenes clones present in farm environment. Despite testing small volumes (25 mL) of milk, we were able to isolate both STEC and Campylobacter spp. directly from raw milk. A proportion of 3% of the bulk tank milk and teat milk samples were contaminated by Campylobacter spp. and one STEC was isolated from bulk tank milk. L monocytogenes was not detected in bulk tank milk, nor in teat milk samples. The agricultural evolvement during the past decades have led to larger production units and new food safety challenges. Dairy cattle production in Norway is in a current transition from tie-stall housing with conventional pipeline milking systems, to modern loose housing systems with robotic milking. The occurrence of the three pathogens in this project were higher in samples collected from farms with loose housing compared to those with tiestall housing. Pasteurization of cow’s milk is a risk reducing procedure to protect consumers from microbial pathogens and in most EU countries, commercial distribution of unpasteurized milk is legally restricted. Together, the results presented in this thesis show that the animal housing may influence the level of pathogenic bacteria in the raw milk and that ingestion of Norwegian raw cow’s milk may expose consumers to pathogenic bacteria which can cause severe disease, especially in children, elderly and in persons with underlying diseases. The results also highlight the importance of storing raw milk at low temperatures between milking and consumption.Å spise mat som er mindre prosessert og mer «naturlig» er en pĂ„gĂ„ende trend i Norge og i andre deler av verden. Interessen for Ă„ drikke melk som ikke er varmebehandlet, sĂ„kalt rĂ„ melk, er ogsĂ„ Ăžkende. I Norge er det pĂ„budt Ă„ pasteurisere melk fĂžr kommersielt salg for Ă„ beskytte forbrukeren mot sykdomsfremkallende mikroorganismer. Fagfolk advarer mot Ă„ drikke rĂ„ melk, og pĂ„peker risikoen for Ă„ bli syk av patogene bakterier som kan finnes i melken. I denne avhandlingen undersĂžker vi den potensielle risikoen det medfĂžrer Ă„ drikke upasteurisert melk fra Norge. I tillegg til Ă„ samle inn tankmelk- og speneprĂžver fra melkegĂ„rder i sĂžrĂžst Norge, samlet vi ogsĂ„ miljĂžprĂžver fra de samme gĂ„rdene for Ă„ kartlegge forekomst og for Ă„ identifisere potensielle mattrygghetsrisikoer i melkeproduksjonen. Alle prĂžvene ble analysert for de zoonotiske sykdomsfremkallende bakteriene Listeria monocytogenes, Campylobacter spp., og Shiga toksin-produserende Escherichia coli (STEC). Kyr kan vĂŠre friske smittebĂŠrere av disse bakteriene, som dermed kan etablere et reservoar pĂ„ gĂ„rdene. Bakteriene kan overfĂžres fra gĂ„rdsmiljĂžet til melkekjeden og dermed utfordre mattryggheten. Disse bakteriene har forĂ„rsaket melkebĂ„rne sykdomsutbrudd over hele verden, ogsĂ„ i Norge. Campylobacter spp. og STEC har lav infeksiĂžs dose, som vil si at man kan bli syk selv om man bare inntar et lavt antall bakterieceller. L. monocytogenes kan gi sykdommen listeriose, en av de mest alvorlige matbĂ„rne zoonotiske sykdommene vi har i den vestlige verden. Resultater fra denne oppgaven viser en hĂžy forekomst av de tre patogenene i gĂ„rdsmiljĂžet. I tillegg var 7% av melkefiltrene vi testet positive for STEC, 13% positive for L. monocytogenes og 4% positive for Campylobacter spp.. Fire av STEC isolatene bar genet for Intimin, eae, som er ansett som en viktig virulensfaktor som Ăžker sjansen for alvorlig sykdom. Ett av de eae-positive isolatene ble funnet i et melkefilter, noe som indikerer at norsk rĂ„ melk kan inneholde patogene STEC. For Ă„ videre vurdere risikoen for Ă„ bli syk av STEC fra rĂ„ melk undersĂžkte vi hvordan de fire eae-positive isolatene vokste i rĂ„ melk lagret ved forskjellige temperaturer. For alle isolatene Ăžkte antall bakterier etter lagring ved 8°C, og for et isolat var veksten signifikant. Etter lagring ved 6°C ble antallet bakterier redusert de fĂžrste 24 timene, deretter stoppet reduksjonen i antall bakterier. Disse resultatene viser hvor viktig det er Ă„ ha stabil lav lagringstemperatur for rĂ„ melk, helst < 4°C. L. monocytogenes isolatene som ble samlet inn fra melkegĂ„rdene viste genetiske likheter med isolater samlet inn fra urbane og rurale miljĂžer rundt omkring i Norge. Derimot var kloner som dominerte i landbruksmiljĂžet forskjellige fra kliniske isolater og isolater fra matproduksjonslokaler. Videre sĂ„ man at en klone kan persistere pĂ„ en gĂ„rd over tid og at melk kan kontamineres av L. monocytogenes kloner som er til stede i gĂ„rdsmiljĂžet. Til tross for smĂ„ testvolum av tankmelken (25 mL) fant vi bĂ„de STEC og Campylobacter spp. i melkeprĂžvene. 3% av tankmelkprĂžvene og speneprĂžvene var positive for Campylobacter spp. og ett STEC isolat ble funnet i tankmelk. L. monocytogenes ble ikke funnet direkte i melkeprĂžvene. Landbruket i Norge er i stadig utvikling der besetningene blir stĂžrre, men fĂŠrre. Melkebesetningene er midt i en overgang der tradisjonell oppstalling med melking pĂ„ bĂ„s byttes ut med lĂžsdriftssystemer og melkeroboter. Forekomsten av de tre patogenene funnet i denne studien var hĂžyere i besetningene med lĂžsdrift sammenliknet med besetningene som hadde melkekyrne oppstallet pĂ„ bĂ„s. Pasteurisering er et viktig forebyggende tiltak for Ă„ beskytte konsumenter fra mikrobielle patogener, og i de fleste EU-land er kommersielt salg av rĂ„ melk juridisk begrenset. Denne studien viser at oppstallingstype kan pĂ„virke nivĂ„ene av patogene bakterier i gĂ„rdsmiljĂžet og i rĂ„ melk. Inntak av rĂ„ melk kan eksponere forbruker for patogene bakterier som kan gi alvorlig sykdom, spesielt hos barn, eldre og personer med underliggende sykdommer. Resultatene underbygger viktigheten av Ă„ pasteurisere melk for Ă„ sikre mattryggheten, og at det er avgjĂžrende Ă„ lagre rĂ„ melk ved kontinuerlig lave temperaturer for Ă„ forebygge vekst av zoonotiske patogener

    3d mirror symmetry of braided tensor categories

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    We study the braided tensor structure of line operators in the topological A and B twists of abelian 3d N=4\mathcal{N}=4 gauge theories, as accessed via boundary vertex operator algebras (VOA's). We focus exclusively on abelian theories. We first find a non-perturbative completion of boundary VOA's in the B twist, which start out as certain affine Lie superalebras; and we construct free-field realizations of both A and B-twist VOA's, finding an interesting interplay with the symmetry fractionalization group of bulk theories. We use the free-field realizations to establish an isomorphism between A and B VOA's related by 3d mirror symmetry. Turning to line operators, we extend previous physical classifications of line operators to include new monodromy defects and bound states. We also outline a mechanism by which continuous global symmetries in a physical theory are promoted to higher symmetries in a topological twist -- in our case, these are infinite one-form symmetries, related to boundary spectral flow, which structure the categories of lines and control abelian gauging. Finally, we establish the existence of braided tensor structure on categories of line operators, viewed as non-semisimple categories of modules for boundary VOA's. In the A twist, we obtain the categories by extending modules of symplectic boson VOA's, corresponding to gauging free hypermultiplets; in the B twist, we instead extend Kazhdan-Lusztig categories for affine Lie superalgebras. We prove braided tensor equivalences among the categories of 3d-mirror theories. All results on VOA's and their module categories are mathematically rigorous; they rely strongly on recently developed techniques to access non-semisimple extensions.Comment: 158 pages, comments welcome

    Learning disentangled speech representations

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    A variety of informational factors are contained within the speech signal and a single short recording of speech reveals much more than the spoken words. The best method to extract and represent informational factors from the speech signal ultimately depends on which informational factors are desired and how they will be used. In addition, sometimes methods will capture more than one informational factor at the same time such as speaker identity, spoken content, and speaker prosody. The goal of this dissertation is to explore different ways to deconstruct the speech signal into abstract representations that can be learned and later reused in various speech technology tasks. This task of deconstructing, also known as disentanglement, is a form of distributed representation learning. As a general approach to disentanglement, there are some guiding principles that elaborate what a learned representation should contain as well as how it should function. In particular, learned representations should contain all of the requisite information in a more compact manner, be interpretable, remove nuisance factors of irrelevant information, be useful in downstream tasks, and independent of the task at hand. The learned representations should also be able to answer counter-factual questions. In some cases, learned speech representations can be re-assembled in different ways according to the requirements of downstream applications. For example, in a voice conversion task, the speech content is retained while the speaker identity is changed. And in a content-privacy task, some targeted content may be concealed without affecting how surrounding words sound. While there is no single-best method to disentangle all types of factors, some end-to-end approaches demonstrate a promising degree of generalization to diverse speech tasks. This thesis explores a variety of use-cases for disentangled representations including phone recognition, speaker diarization, linguistic code-switching, voice conversion, and content-based privacy masking. Speech representations can also be utilised for automatically assessing the quality and authenticity of speech, such as automatic MOS ratings or detecting deep fakes. The meaning of the term "disentanglement" is not well defined in previous work, and it has acquired several meanings depending on the domain (e.g. image vs. speech). Sometimes the term "disentanglement" is used interchangeably with the term "factorization". This thesis proposes that disentanglement of speech is distinct, and offers a viewpoint of disentanglement that can be considered both theoretically and practically

    Chromosome Abnormalities in Hematological Malignancies and Its Clinical Significance

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    The latest version of the World Health Organization guidelines focuses mainly on the genetic and cytogenetic features of hematologic neoplasms as predictors of diagnostic, treatment decision, prognostic outcome, and for treatment monitoring in hematological malignancies. There are different techniques to identify these abnormalities. Live cells are needed for chromosome preparation. The Hematological malignancies include myeloid and lymphoid neoplasms. The myeloid neoplasms include Myelodysplastic syndromes, myeloproliferative neoplasms, and acute myeloid leukemias. The Lymphoid neoplasms include acute and chronic lymphocytic leukemias, plasma cell neoplasms, myeloma, hodgkin, and non-hodgkin lymphomas. The first chromosomal abnormality discovered in connection with cancer is the Philadelphia chromosome, which is an abnormal chromosome 22, formed due to the translocation between chromosomes 9 and 22. The presence of this abnormal chromosome confirms the diagnosis of “CML”. After that, hundreds of chromosomal abnormalities have been identified in hematological malignancies in different parts of the world. In AML, specific abnormalities were identified as having a good prognosis, intermediate prognosis, and poor prognosis. In other hematological malignancies also there some specific chromosome abnormalities are associated with prognostication. Now a day’s clinicians depend mainly on genetic abnormalities for the proper treatment management of hematological malignancies, so the study of chromosomal abnormalities is essential

    Epigenetics : a catalyst of plant immunity against pathogens

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    The plant immune system protects against pests and diseases. The recognition of stress-related molecular patterns triggers localised immune responses, which are often followed by longer-lasting systemic priming and/or up-regulation of defences. In some cases, this induced resistance (IR) can be transmitted to following generations. Such transgenerational IR is gradually reversed in the absence of stress at a rate that is proportional to the severity of disease experienced in previous generations. This review outlines the mechanisms by which epigenetic responses to pathogen infection shape the plant immune system across expanding time scales. We review the cis- and trans-acting mechanisms by which stress-inducible epigenetic changes at transposable elements (TEs) regulate genome-wide defence gene expression and draw particular attention to one regulatory model that is supported by recent evidence about the function of AGO1 and H2A.Z in transcriptional control of defence genes. Additionally, we explore how stress-induced mobilisation of epigenetically controlled TEs acts as a catalyst of Darwinian evolution by generating (epi)genetic diversity at environmentally responsive genes. This raises questions about the long-term evolutionary consequences of stress-induced diversification of the plant immune system in relation to the long-held dichotomy between Darwinian and Lamarckian evolution
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