Metagenomics for Bacteriology

The study of bacteria, or bacteriology, has gone through transformative waves since its inception in the 1600s. It all started by the visualization of bacteria using light microscopy by Antonie van Leeuwenhoek, when he first described “animalcules.” Direct cellular observation then evolved into utilizing different wavelengths on novel platforms such as electron, fluorescence, and even near-infrared microscopy. Understanding the link between microbes and disease (pathogenicity) began with the ability to isolate and cultivate organisms through aseptic methodologies starting in the 1700s. These techniques became more prevalent in the following centuries with the work of famous scientists such as Louis Pasteur and Robert Koch, and many others since then. The relationship between bacteria and the host’s immune system was first inferred in the 1800s, and to date is continuing to unveil its mysteries. During the last century, researchers initiated the era of molecular genetics. The discovery of the first-generation sequencing technology, the Sanger method, and, later, the polymerase chain reaction technology propelled the molecular genetics field by exponentially expanding the knowledge of relationship between gene structure and function. The rise of commercially available next-generation sequencing methodologies, in the beginning of this century, is drastically allowing larger amount of information to be acquired, in a manner open to the democratization of the approach

The bacteriology of ''clean rooms'' progress report, 1 oct. 1964 - 31 mar. 1965

Clean room bacteriology - ambient air and bench top microbial contamination levels, and degree of environmental control in production area

Detection Of Salmonella Spp. By Conventional Bacteriology And By Quantitative Polymerase-chain Reaction In Commercial Egg Structures

Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.5% (1/22). The qPCR method provided better detection of Salmonella spp. in commercial eggs than conventional bacteriology. The conventional egg washing and disinfection procedures are not efficient to eliminate Salmonella. © 2016, Fundacao APINCO de Ciencia e Tecnologia Avicolas. All rights reserved.18111712

New Book

Veterinary Bacteriology, I. A. Merchant, D.V.M., Ph.D., C.P.H., Associate Professor of Veterinary Bacteriology and Hygiene, Iowa State College. 1st edition. Cloth bound, 636 pages, 135 illustrations. Collegiate Press, Inc., Ames, Iowa. 1940. Price $7.00

Practical Diagnostic Bacteriology

Although there is a growing awareness . of the value of diagnostic bacteriology by practicing veterinarians, the benefits that can be gai11ed :by \u27practical diagnostic methods are often unutilized. Perhaps one reason practitioners shy away from using bacteriology as a diagnostic tool is because of the misconceptio11 that the identification of pathogenic organisms is extremely complex an,d requires a vast amount of specialized equipment. It i3 hoped that this discussion ,will arouse further interest in diagnostic bacteriology and show its applicability to the veterinarian\u27s office

Teaching the Elementary Course in Bacteriology

The objectives to be emphasized in courses in bacteriology in the probable order of importance for the elementary course are: A. Vocational Practical Utilization in vocation. B. Health. With some student groups rank first. C. Citizenship. Social-Civic. D. Avocationa. Of minor importance in bacteriology in most cases

Department of Animal and Veterinary Sciences (University of Maine) Records, 1921-1978

The records mainly contain textual information created and curated by the University of Maine Department of Animal and Veterinary Sciences (also known as the Department of Bacteriology and Veterinary Sciences and Department of Bacteriology). The record series Disease Record Cards contains Pullorum disease records for various flocks of birds across Maine.https://digitalcommons.library.umaine.edu/findingaids/1399/thumbnail.jp