Predictive modeling in food mycology using adaptive neuro-fuzzy systems

Fungal growth leads to spoilage of food and animal feeds and to formation of mycotoxins and potentially allergenic spores. There is a growing interest in predictive modeling microbial growth as an alternative to time consuming traditional, microbiological enumeration techniques. Several statistical models have been accounted to describe the growth of different micro-organisms. However neural networks, as highly nonlinear approximator scheme, have the potential of modeling some complex, phenomena better than the others. The application of adaptive neuro-fuzzy systems in predictive microbiology is presented in this paper. This technique is used to build up a model of the joint effect of water-activity, pH level and temperature to predict the maximum specific growth rate of the Ascomycetous Fungus Monascus Ruber. The proposed scheme is compared against standard neural network approaches. Neuro-fuzzy systems offer an alternative and powerful technique to model microbial kinetic parameters and could thus become an efficient tool in predictive mycology

Modeling growth of specific spoilage organisms in tilapia: Comparison Baranyi with chi-square automatic interaction detection (CHAID) model

Tilapia is an important aquatic fish, but severe spoilage of tilapia is most likely related to the global aquaculture. The spoilage is mostly caused by specific spoilage organisms (SSO). Therefore, it is very important to use microbial models to predict the growth of SSO in tilapia. This study firstly verified Pseudomonas and Vibrio as the SSO of tilapia, then established microbial growth models based on Baranyi and chi-square automatic interaction detection (CHAID) models and compared their effectiveness. The results showed that both Baranyi model and CHAID model are appropriate for predicting the growth of microorganism. Baranyi model fits the microorganism growth better than CHAID model overall though CHAID model fits well at stationary phase. CHAID model predicts the microorganism growth accurately when the rate of change of the experiment data is big.Key words: Specific spoilage organisms (SSO), tilapia, chi-square automatic interaction detection (CHAID), Baranyi, shelf-life

Dynamic non-linear system modelling using wavelet-based soft computing techniques

The enormous number of complex systems results in the necessity of high-level and cost-efficient modelling structures for the operators and system designers. Model-based approaches offer a very challenging way to integrate a priori knowledge into the procedure. Soft computing based models in particular, can successfully be applied in cases of highly nonlinear problems. A further reason for dealing with so called soft computational model based techniques is that in real-world cases, many times only partial, uncertain and/or inaccurate data is available. Wavelet-Based soft computing techniques are considered, as one of the latest trends in system identification/modelling. This thesis provides a comprehensive synopsis of the main wavelet-based approaches to model the non-linear dynamical systems in real world problems in conjunction with possible twists and novelties aiming for more accurate and less complex modelling structure. Initially, an on-line structure and parameter design has been considered in an adaptive Neuro- Fuzzy (NF) scheme. The problem of redundant membership functions and consequently fuzzy rules is circumvented by applying an adaptive structure. The growth of a special type of Fungus (Monascus ruber van Tieghem) is examined against several other approaches for further justification of the proposed methodology. By extending the line of research, two Morlet Wavelet Neural Network (WNN) structures have been introduced. Increasing the accuracy and decreasing the computational cost are both the primary targets of proposed novelties. Modifying the synoptic weights by replacing them with Linear Combination Weights (LCW) and also imposing a Hybrid Learning Algorithm (HLA) comprising of Gradient Descent (GD) and Recursive Least Square (RLS), are the tools utilised for the above challenges. These two models differ from the point of view of structure while they share the same HLA scheme. The second approach contains an additional Multiplication layer, plus its hidden layer contains several sub-WNNs for each input dimension. The practical superiority of these extensions is demonstrated by simulation and experimental results on real non-linear dynamic system; Listeria Monocytogenes survival curves in Ultra-High Temperature (UHT) whole milk, and consolidated with comprehensive comparison with other suggested schemes. At the next stage, the extended clustering-based fuzzy version of the proposed WNN schemes, is presented as the ultimate structure in this thesis. The proposed Fuzzy Wavelet Neural network (FWNN) benefitted from Gaussian Mixture Models (GMMs) clustering feature, updated by a modified Expectation-Maximization (EM) algorithm. One of the main aims of this thesis is to illustrate how the GMM-EM scheme could be used not only for detecting useful knowledge from the data by building accurate regression, but also for the identification of complex systems. The structure of FWNN is based on the basis of fuzzy rules including wavelet functions in the consequent parts of rules. In order to improve the function approximation accuracy and general capability of the FWNN system, an efficient hybrid learning approach is used to adjust the parameters of dilation, translation, weights, and membership. Extended Kalman Filter (EKF) is employed for wavelet parameters adjustment together with Weighted Least Square (WLS) which is dedicated for the Linear Combination Weights fine-tuning. The results of a real-world application of Short Time Load Forecasting (STLF) further re-enforced the plausibility of the above technique

Comparative Analysis of Machine Learning Methods to Predict Growth of F. sporotrichioides and Production of T-2 and HT-2 Toxins in Treatments with Ethylene-Vinyl Alcohol Films Containing Pure Components of Essential Oils

The efficacy of ethylene-vinyl alcohol copolymer films (EVOH) incorporating the essential oil components cinnamaldehyde (CINHO), citral (CIT), isoeugenol (IEG), or linalool (LIN) to control growth rate (GR) and production of T-2 and HT-2 toxins by Fusarium sporotrichioides cultured on oat grains under different temperature (28, 20, and 15 °C) and water activity (aw) (0.99 and 0.96) regimes was assayed. GR in controls/treatments usually increased with increasing temperature, regardless of aw, but no significant differences concerning aw were found. Toxin production decreased with increasing temperature. The effectiveness of films to control fungal GR and toxin production was as follows: EVOH-CIT > EVOH-CINHO > EVOH-IEG > EVOH-LIN. With few exceptions, effective doses of EVOH-CIT, EVOH-CINHO, and EVOH-IEG films to reduce/inhibit GR by 50%, 90%, and 100% (ED50, ED90, and ED100) ranged from 515 to 3330 µg/culture in Petri dish (25 g oat grains) depending on film type, aw, and temperature. ED90 and ED100 of EVOH-LIN were >3330 µg/fungal culture. The potential of several machine learning (ML) methods to predict F. sporotrichioides GR and T-2 and HT-2 toxin production under the assayed conditions was comparatively analyzed. XGBoost and random forest attained the best performance, support vector machine and neural network ranked third or fourth depending on the output, while multiple linear regression proved to be the worst

Application of an electronic nose coupled with fuzzy-wavelet network for the detection of meat spoilage

Food product safety is one of the most promising areas for the application of electronic noses. During the last twenty years, these sensor-based systems have made odour analyses possible. Their application into the area of food is mainly focused on quality control, freshness evaluation, shelf-life analysis and authenticity assessment. In this paper, the performance of a portable electronic nose has been evaluated in monitoring the spoilage of beef fillets stored either aerobically or under modified atmosphere packaging, at different storage temperatures. A novel multi-output fuzzy wavelet neural network model has been developed, which incorporates a clustering pre-processing stage for the definition of fuzzy rules. The dual purpose of the proposed modelling approach is not only to classify beef samples in the relevant quality class (i.e. fresh, semi-fresh and spoiled), but also to predict their associated microbiological population. Comparison results against advanced machine learning schemes indicated that the proposed modelling scheme could be considered as a valuable detection methodology in food microbiology

Radial basis function neural networks for modeling growth rates of the basidiomycetes Physisporinus vitreus and Neolentinus lepideus

A radial basis function (RBF) neural network was developed and compared against a quadratic response surface (RS) model for predicting the specific growth rates of the biotechnologically important basidiomycetous fungi, Physisporinus vitreus and Neolentinus lepideus, under three environmental conditions: temperature (10-30°C), water activity (0.950-9.998), and pH (4-6). Both the RBF network and polynomial RS model were mathematically evaluated against experimental data using graphical plots and several statistical indices. The evaluation showed that both models gave reasonably good predictions, but the performance of the RBF neural network was superior to that of the classical statistical method for all three data sets used (training, testing, full). Sensitivity analysis revealed that of the three experimental factors the most influential on the growth rate of P. vitreus was water activity, followed by temperature and pH to a lesser extent. In contrast, temperature in particular and then water activity were the key determinants of the development of N. lepideus. RBF neural networks could be a powerful technique for modeling fungal growth behavior under certain parameters and an alternative to time-consuming, traditional microbiological technique

A machine learning taxonomic classifier for science publications

Dissertação de mestrado integrado em Engineering and Management of Information SystemsThe evolution in scientific production, associated with the growing interdomain collaboration of knowledge and the increasing co-authorship of scientific works remains supported by processes of manual, highly subjective classification, subject to misinterpretation. The very taxonomy on which this same classification process is based is not consensual, with governmental organizations resorting to taxonomies that do not keep up with changes in scientific areas, and indexers / repositories that seek to keep up with those changes. We find a reality distinct from what is expected and that the domains where scientific work is recorded can easily be misrepresentative of the work itself. The taxonomy applied today by governmental bodies, such as the one that regulates scientific production in Portugal, is not enough, is limiting, and promotes classification in areas close to the desired, therefore with great potential for error. An automatic classification process based on machine learning algorithms presents itself as a possible solution to the subjectivity problem in classification, and while it does not solve the issue of taxonomy mismatch this work shows this possibility with proved results. In this work, we propose a classification taxonomy, as well as we develop a process based on machine learning algorithms to solve the classification problem. We also present a set of directions for future work for an increasingly representative classification of evolution in science, which is not intended as airtight, but flexible and perhaps increasingly based on phenomena and not just disciplines.A evolução na produção de ciência, associada à crescente colaboração interdomínios do conhecimento e à também crescente coautoria de trabalhos permanece suportada por processos de classificação manual, subjetiva e sujeita a interpretações erradas. A própria taxonomia na qual assenta esse mesmo processo de classificação não é consensual, com organismos estatais a recorrerem a taxonomias que não acompanham as alterações nas áreas científicas, e indexadores/repositórios que procuram acompanhar essas mesmas alterações. Verificamos uma realidade distinta do espectável e que os domínios onde são registados os trabalhos científicos podem facilmente estar desenquadrados. A taxonomia hoje aplicada pelos organismos governamentais, como o caso do organismo que regulamenta a produção científica em Portugal, não é suficiente, é limitadora, e promove a classificação em domínios aproximados do desejado, logo com grande potencial para erro. Um processo de classificação automática com base em algoritmos de machine learning apresenta-se como uma possível solução para o problema da subjetividade na classificação, e embora não resolva a questão do desenquadramento da taxonomia utilizada, é apresentada neste trabalho como uma possibilidade comprovada. Neste trabalho propomos uma taxonomia de classificação, bem como nós desenvolvemos um processo baseado em machine learning algoritmos para resolver o problema de classificação. Apresentamos ainda um conjunto de direções para trabalhos futuros para uma classificação cada vez mais representativa da evolução nas ciências, que não pretende ser hermética, mas flexível e talvez cada vez mais baseada em fenómenos e não apenas em disciplinas