Correlated NanoSIMS, TEM, and XANES Studies of Presolar Grains

The objective of this thesis is to describe the correlated study of individual presolar grains via Nano-scale Secondary Ion Mass Spectrometry (NanoSIMS), Transmission Electron Microscopy (TEM), and Scanning Transmission X-ray Microscopy (STXM) utilizing X-ray Absorption Near Edge Structure (XANES), with a focus on connecting these correlated laboratory studies to astrophysical phenomena. The correlated isotopic, chemical, and microstructural studies of individual presolar grains provide the most detailed description of their formation environments, and help to inform astrophysical models and observations of stellar objects. As a part of this thesis I have developed and improved upon laboratory techniques for micromanipulating presolar grains and embedding them in resin for ultramicrotomy after NanoSIMS analyses and prior to TEM characterization. The new methods have yielded a 100% success rate and allow for the specific correlation of microstructural and isotopic properties of individual grains. Knowing these properties allows for inferences to be made regarding the condensation sequences and the origins of the stellar material that condensed to form these grains. NanoSIMS studies of ultramicrotomed sections of presolar graphite grains have revealed complex isotopic heterogeneities that appear to be primary products of the grains\u27 formation environments and not secondary processing during the grains\u27 lifetimes. Correlated excesses in 15N and 18O were identified as being carried by TiC subgrains within presolar graphite grains from supernovae (SNe). These spatially-correlated isotopic anomalies pinpoint the origin of the material that formed these grains: the inner He/C zone. Complex microstructures and isotopic heterogeneities also provide evidence for mixing in globular SN ejecta, which is corroborated by models and telescopic observations. In addition to these significant isotopic discoveries, I have also observed the first reported nanocrystalline core surrounded by turbostratic graphite within a low-density SN graphite grain. Nanocrystalline cores consisting of randomly-oriented 2-4 nm sheets of graphene and surrounded by concentric shells of graphite have been observed in high-density presolar graphite grains from Asymptotic Giant Branch stars, whose grains are typically microstructurally distinct from SN graphite grains. These vastly different stellar environments briefly formed similar nanocrystalline structures before diverging in the structure of their mantling graphite to be typical of AGB and SN grains. While relatively few correlated NanoSIMS and TEM studies have been performed previously, which this research thesis aims to expand, my collaborators and I also endeavored to add a third correlated technique, STXM/XANES, which had previously not been applied to presolar grains. XANES allows for the investigation of molecular bonds, which we used to help infer physical and chemical properties of stellar ejecta. I investigated the C K-edge and Ti L-edge of molecular bonds in both presolar graphite grains and their TiC subgrains. The presolar graphite grains, while overwhelmingly composed of aromatic C molecules, host a wide variety of minor organic molecules. Considering the large isotopic anomalies in the grains, these minor components are not likely due to contamination. I also investigated the valence state of Ti in Ti-rich subgrains and plan to work towards illuminating the effect that V in solid solution has upon the TiC bonds

Probabilistic Protein Design, Comparative Modeling, and the Structure of a Multidomain P53 Oligomer Bound to DNA

Proteins are the main functional components of all cellular processes, and most of them fold into unique three-dimensional shapes guided by their amino-acid sequence. Discovering the structure of a protein, or protein complexes, can provide important clues about how they perform their function. However, the chemical, physical or architectural properties of many proteins impede traditional approaches to structure determination. Two such proteins, the tumor suppressor p53 and the cholesterol processing enzyme endothelial lipase, are prime examples of problematic proteins that defy structural investigation via crystallographic methods. Therefore, new techniques must be developed to gain valuable structural insights, such as: computationally assisted protein design strategies, more efficient crystal screening, or a combination of both. We applied a statistical computationally assisted design strategy to stabilize a p53 variant consisting of two independently folding domains. The re-engineered variant retained normal DNA-binding activities, and allowed us to experimentally determine the first structure of a physiologically active multi-domain p53 tetramer bound to a full-length DNA response element. We then demonstrated how computational methodology can be used to gain functional detail of proteins in the absence of experimentally determined structures. By creating comparative models of endothelial lipase, we discovered structural features that describe function and regulation, and gained a better understanding of the mechanisms conferring substrate specificity. Additionally, traditional methods for protein structure determination, such as X-ray crystallography, require relatively large amounts of purified sample in order to screen a sufficient variety of conditions. To improve this process, we developed a novel method for protein crystal screening using a microfluidics platform. We show how it is possible to use smaller quantities of protein to screen larger varieties of conditions, in turn increasing the probability of success in obtaining crystals. Furthermore, in contrast to current crystallographic approaches, all steps from screening to crystal growth to data collection were performed within the same reaction chamber, without any manipulation of the crystal, dramatically increasing the efficiency of both time and sample required to realize the structure. Collectively, these results demonstrate how advances in computational and experimental approaches can provide structural detail for proteins in circumstances where traditional methodology fails

Malignant melanoma of the urethra: a rare histologic subdivision of vulvar cancer with a poor prognosis

Malignant melanoma of the urethra is a rare tumour that is difficult to diagnose and treat, resulting in a poor prognosis. In this paper, we present the case of a 65-year-old woman who was referred to a gynaecologist because of a urethral mass that mimicked a caruncle. The tumour was removed by local excision, and a pathological analysis revealed a malignant melanoma. Distal urethrectomy was performed after three months with no evidence of residual tumour. There was no evidence of disease at a six-year followup. In this paper, we compare the epidemiology, treatment, staging, and prognosis of vulvar cancer in general to malignant melanoma of the vulva in particular

Natural Fiber Based Composites

Entitled “Natural Fiber-Based Composites”, this Special Issue has the objective to give an inventory of the latest research in the area of composites reinforced with natural fibers. Fibers of renewable origin have many advantages. They are abundant and cheap, they have a reduced impact on the environment, and they are also independent from fossil resources. Their ability to mechanically reinforce thermoplastic matrices is well known, as their natural heat insulation ability. In the last twenty years, the use of cellulosic and lignocellulosic agricultural by-products for composite applications has been of great interest, especially for reinforcing matrices. The matrices can themselves be of renewable origin (e.g., proteins, starch, polylactic acid, polyhydroxyalkanoates, polyamides, etc.), thus contributing to the development of 100% bio-based composites with a controlled end of life. This Special Issue’s objective is to give an inventory of the latest research in this area of composites reinforced with natural fibers, focusing in particular on the preparation and molding processes of such materials (e.g., extrusion, injection-molding, hot pressing, etc.) and their characterization. It contains one review and nineteen research reports authored by researchers from four continents and sixteen countries, namely, Brazil, China, France, Italy, Japan, Malaysia, Mexico, Pakistan, Poland, Qatar, Serbia, Slovenia, Spain, Sweden, Tunisia, and Vietnam. It provides an update on current research in the field of natural fiber based composite materials. All these contributions will be a source of inspiration for the development of new composites, especially for producers of natural fibers, polymer matrices of renewable origin and composite materials. Generally speaking, these new materials are environmentally friendly and will undoubtedly find numerous applications in the years to come in many sectors. Dr. Philippe Evon Guest Edito

Integrative Assessment and Modelling of the Non Timber Forest Products Potential in Nuba Mountains of Sudan by Field Methods, Remote Sensing and GIS

Pressure imposed at any one place or point in time results in a complexity of spatial and temporal interactions within topographical ecosystems. It can be propagated through the system and may have implications for future ecosystem functions over a wide array of various spatial and temporal scales. Under conditions of wars and other socio-economic conflicts, these processes are most forceful in developing countries amidst declining economic growth, lack of awareness, deterioration of ecosystem services, loss of existing traditional knowledge bases and weak governance structures. Forests are an essential part of ecosystem services, not only as a resource but as a contributor to biological systems as well. They represent one of the most important sectors in the context of Environmental Change (EC), both from the point of mitigation as well as adaptation. While forests are projected to be adversely impacted under EC, they are also providing opportunities to mitigate these changes. Yet this is one of the least understood sectors, especially at the regional level - many of its fundamental metrics such as mitigation potential, vulnerability and the likely impacts of EC are still not well understood until now. Thus, there is a need for research and field investigations into the synergy of mitigation and adaptation so that the cost of addressing EC impacts can be reduced and the co-benefits can be increased. The aim of this study is to focus particularly on forest-based ecosystem services and to use forests as a strategy for inducing environmental change within the Nuba Mountains in Sudan, specifically for systems in poor condition under EC, and furthermore to explore forests as an entry point for investigating the relationship between urban and rural development and ecosystem services. In addition, the aim is also to raise understanding of the relations between patterns of local-level economic and demographic changes, the nature and value of local ecosystem services, and the role of such services in increasingly interlinked urban and rural livelihood systems. The methodology applied in the current research is three-pronged: a formal literature review, a socio–economic survey (based on semi-structured interviews of household heads via Rapid Rural Appraisal (RRA), with a focus on group discussions, informal meetings, free listening and key informant techniques), and multitemporal optical satellite data analysis (i.e. Landsat and RapidEye). Landsat imagery was utilized to gather the spatial characteristics of the region and to study the Land Use/Land Cover (LU/LC) changes during the period from 1984 to 2014. Meanwhile, RapidEye imagery was used to generate the tree species distribution map. Qualitative and quantitative techniques were applied to analyze socio-economic data. Moreover, Food Consumption Score (FCS) was used to gauge both diversity and frequency of food consumption in surveyed areas. Geographic object-based image analysis (i.e. K-Nearest Neighbour classifier and knowledge-based classifiers) based on a developed model of integrated features (such as vegetation indices, DEM, thematic layers and meteorological information) was applied. Post Classification Analysis (PCA) as well as Post Change Detection (PCD) techniques were used. Hotspot analysis was conducted to detect the areas affected by deforestation. Furthermore, Ordinary Least Squares regression (OLS), Autocorrelation (Moran's) analysis, and Geographically Weighted Regression analyses (GWR) were applied to address the interaction of the different socioeconomic/ecological factors on Non Timber Forest Products (NTFPs) collection and to simulate the dependency scenarios of NTFPs along with their impact on poverty alleviation. Additionally, simulation was performed to estimate the future forest density and predict the dependency on forest services. An increasing impact of intensive interactions between the rural and urban areas has long been acknowledged. However, recent changes in the global political economy and environmental systems, as well as local dynamics of the study area driven by war, drought and deforestation, have led to an increasing rapidity and depth in rural transformation, as well as to a significant impact on urban areas. Like most environmental problems, the effects of these drivers are complex and are stressed diversely across different geographic regions by the socio-political processes that underlie recent economic and cultural globalization. These interactions and processes have increasingly brought rapid changes in land cover, social, institutional and livelihood transformation across broad areas of South Kordofan. Moreover, the study unveils new dynamics such as high rates of migration and mobility by the indigenous population and the increasing domination of market-centric livelihoods in many villages that were once dominated by rural agricultural and natural resourcesbased socio-economic systems. Furthermore, the research highlights the significant roles of NTFPs and trees in contributing to Nuba Mountains’ economic development, food security and environmental health, indicating which requirements need to be addressed in order to improve these potentials. The study proves that drawing on a wide range of these products for livelihood strengthens rural people’s ability to deal with and adapt to both EC and extreme events. Moreover, the results underline the importance of participatory approaches of rural women and their impact on NTFPs management with recommendations of more emphasis on potential roles and the ability of women to participate in public fora. Furthermore, the study shows that the use of high-resolution satellite imagery, integrated with model-based terrestrial information, provides a precise knowledge about the magnitude and distribution of LU/LC patterns. These methods can make an important contribution towards a better understanding of EC dynamics over time. The study reveals that more information exchange is needed to inform actors and decision makers regarding specific experiences, capacity gaps and knowledge to address EC. Subsequently, new policies and strategies are required to much more specifically focus on how to deal with consequences of longer-term EC rather than with the impacts of sudden natural disasters

Cumulative index to NASA Tech Briefs, 1986-1990, volumes 10-14

Tech Briefs are short announcements of new technology derived from the R&D activities of the National Aeronautics and Space Administration. These briefs emphasize information considered likely to be transferrable across industrial, regional, or disciplinary lines and are issued to encourage commercial application. This cumulative index of Tech Briefs contains abstracts and four indexes (subject, personal author, originating center, and Tech Brief number) and covers the period 1986 to 1990. The abstract section is organized by the following subject categories: electronic components and circuits, electronic systems, physical sciences, materials, computer programs, life sciences, mechanics, machinery, fabrication technology, and mathematics and information sciences

Geotechnical Engineering for the Preservation of Monuments and Historic Sites III

The conservation of monuments and historic sites is one of the most challenging problems facing modern civilization. It involves, in inextricable patterns, factors belonging to different fields (cultural, humanistic, social, technical, economical, administrative) and the requirements of safety and use appear to be (or often are) in conflict with the respect of the integrity of the monuments. The complexity of the topic is such that a shared framework of reference is still lacking among art historians, architects, structural and geotechnical engineers. The complexity of the subject is such that a shared frame of reference is still lacking among art historians, architects, architectural and geotechnical engineers. And while there are exemplary cases of an integral approach to each building element with its static and architectural function, as a material witness to the culture and construction techniques of the original historical period, there are still examples of uncritical reliance on modern technology leading to the substitution from earlier structures to new ones, preserving only the iconic look of the original monument. Geotechnical Engineering for the Preservation of Monuments and Historic Sites III collects the contributions to the eponymous 3rd International ISSMGE TC301 Symposium (Naples, Italy, 22-24 June 2022). The papers cover a wide range of topics, which include: 　 - Principles of conservation, maintenance strategies, case histories - The knowledge: investigations and monitoring - Seismic risk, site effects, soil structure interaction - Effects of urban development and tunnelling on built heritage - Preservation of diffuse heritage: soil instability, subsidence, environmental damages The present volume aims at geotechnical engineers and academics involved in the preservation of monuments and historic sites worldwide

European Union Timber Regulation Impact on International Timber Markets

The trade of illegal timber, often from illegal logging, has severe environmental, social and economic consequences. The EU’s response to this problem came with the Forest Law Enforcement, Governance and Trade (FLEGT) Action Plan, with its specific goal to end illegal logging, thereby improving sustainability of forest resources. In March 2013, an additional step was taken by implementing the EU Timber Regulation (EUTR). The EUTR requires proof of timber’s origin and legality to ensure that no illegal timber is imported into the EU. To this end the EU intends to block imports of any wood or wood product which comes from unknown sources. Certification of sustainable forest management will help EU importers minimize risk, which is an essential part of their required due diligence system. Monitoring organizations are established to assist trade associations and businesses to construct comprehensive due diligence systems. National competent authorities are designated to follow the trade of the new FLEGT-licensed timber and timber products. In the first year of the EUTR there are positive impacts, of which the most important is awareness of the disastrous situation with illegal logging, driven by exports of illegal timber. Another positive development is tropical timber exporters documenting the legality of their wood exports. Yet another positive feature is establishment of due diligence systems by EU importers. However, there are considerable problems for ensuring legal trade; for example the lack of comprehensive documentation of origin and legality. Analysis of recent trends establishes changes in the European timber trade in terms of sourcing, substitution, diversion to less-demanding countries. Short-term forecasts of market trends and changes will enable further policy assessment to achieve the objectives of improved legality in international timber markets.JRC.H.3-Forest Resources and Climat

Advances in Wood Composites

Wood composites have shown very good performance, and substantial service lives when correctly specified for the exposure risks present. Selection of an appropriate product for the job should be accompanied by decisions about the appropriate protection, whether this is by design, by preservative treatment or by wood modification techniques. This Special Issue, Advances in Wood Composites presents recent progress in enhancing and refining the performance and properties of wood composites by chemical and thermal modification and the application of smart nanomaterials, which have made them a particular area of interest for researchers. In addition, it reviews some important aspects in the field of wood composites, with particular focus on their materials, applications, and engineering and scientific advances, including solutions inspired biomimetrically by the structure of wood and wood composites. This Special Issue, with a collection of 13 original contributions, provides selected examples of recent Advances in Wood Composite