15,284 research outputs found
Biophotonic Tools in Cell and Tissue Diagnostics.
In order to maintain the rapid advance of biophotonics in the U.S. and enhance our competitiveness worldwide, key measurement tools must be in place. As part of a wide-reaching effort to improve the U.S. technology base, the National Institute of Standards and Technology sponsored a workshop titled "Biophotonic tools for cell and tissue diagnostics." The workshop focused on diagnostic techniques involving the interaction between biological systems and photons. Through invited presentations by industry representatives and panel discussion, near- and far-term measurement needs were evaluated. As a result of this workshop, this document has been prepared on the measurement tools needed for biophotonic cell and tissue diagnostics. This will become a part of the larger measurement road-mapping effort to be presented to the Nation as an assessment of the U.S. Measurement System. The information will be used to highlight measurement needs to the community and to facilitate solutions
The VIMOS Integral Field Unit: data reduction methods and quality assessment
With new generation spectrographs integral field spectroscopy is becoming a
widely used observational technique. The Integral Field Unit of the VIsible
Multi-Object Spectrograph on the ESO-VLT allows to sample a field as large as
54" x 54" covered by 6400 fibers coupled with micro-lenses. We are presenting
here the methods of the data processing software developed to extract the
astrophysical signal of faint sources from the VIMOS IFU observations. We focus
on the treatment of the fiber-to-fiber relative transmission and the sky
subtraction, and the dedicated tasks we have built to address the peculiarities
and unprecedented complexity of the dataset. We review the automated process we
have developed under the VIPGI data organization and reduction environment
(Scodeggio et al. 2005), along with the quality control performed to validate
the process. The VIPGI-IFU data processing environment is available to the
scientific community to process VIMOS-IFU data since November 2003.Comment: 19 pages, 10 figures and 1 table. Accepted for publication in PAS
The XMM-Newton view of the Crab
Aims. We discuss the current X-ray view of the Crab Nebula and Pulsar, summarising our analysis of observations of the source with the EPIC-pn camera on board the XMM-Newton observatory. Different modes of EPIC-pn were combined in order to yield a complete scenario of the spectral properties of the Crab resolved in space and time (pulse phase). In addition we give a description of the special
EPIC-pn Burst mode and guidance for data reduction in that mode.
Methods. We analysed spectra for the nebula and pulsar separately in the 0.6â12.0 keV energy band. All data were processed with the
SAS 6.0.0 XMM-Newton Scientific Analysis System package; models were fitted to the data with XSPEC 11. The high time resolution of EPIC-pn in its Burst mode (7 ÎŒs) was used for a phase resolved analysis of the pulsar spectrum, after determination of the period with epoch folding techniques. Data from the SmallWindow mode were processed and corrected for pile-up allowing for spectroscopy
simultaneously resolved in space and time.
Results. The spatial variation of the spectrum over the entire region of the Crab shows a gradual spectral softening from the inner pulsar region to the outer nebula region with a variation in photon index, Î, from 2.0 to 2.4. Pulse phase resolved spectroscopy of the Crab Pulsar reveals a phase dependent modulation of the photon index in form of a significant hardening of the spectrum in the
inter-peak phase from Î = 1.7 during the pulse peak to Î = 1.5
Review of small-angle coronagraphic techniques in the wake of ground-based second-generation adaptive optics systems
Small-angle coronagraphy is technically and scientifically appealing because
it enables the use of smaller telescopes, allows covering wider wavelength
ranges, and potentially increases the yield and completeness of circumstellar
environment - exoplanets and disks - detection and characterization campaigns.
However, opening up this new parameter space is challenging. Here we will
review the four posts of high contrast imaging and their intricate interactions
at very small angles (within the first 4 resolution elements from the star).
The four posts are: choice of coronagraph, optimized wavefront control,
observing strategy, and post-processing methods. After detailing each of the
four foundations, we will present the lessons learned from the 10+ years of
operations of zeroth and first-generation adaptive optics systems. We will then
tentatively show how informative the current integration of second-generation
adaptive optics system is, and which lessons can already be drawn from this
fresh experience. Then, we will review the current state of the art, by
presenting world record contrasts obtained in the framework of technological
demonstrations for space-based exoplanet imaging and characterization mission
concepts. Finally, we will conclude by emphasizing the importance of the
cross-breeding between techniques developed for both ground-based and
space-based projects, which is relevant for future high contrast imaging
instruments and facilities in space or on the ground.Comment: 21 pages, 7 figure
Genome-inspired molecular identification in organic matter via Raman spectroscopy
Rapid, non-destructive characterization of molecular level chemistry for
organic matter (OM) is experimentally challenging. Raman spectroscopy is one of
the most widely used techniques for non-destructive chemical characterization,
although it currently does not provide detailed identification of molecular
components in OM, due to the combination of diffraction-limited spatial
resolution and poor applicability of peak-fitting algorithms. Here, we develop
a genome-inspired collective molecular structure fingerprinting approach, which
utilizes ab initio calculations and data mining techniques to extract molecular
level chemistry from the Raman spectra of OM. We illustrate the power of such
an approach by identifying representative molecular fingerprints in OM, for
which the molecular chemistry is to date inaccessible using non-destructive
characterization techniques. Chemical properties such as aromatic cluster size
distribution and H/C ratio can now be quantified directly using the identified
molecular fingerprints. Our approach will enable non-destructive identification
of chemical signatures with their correlation to the preservation of
biosignatures in OM, accurate detection and quantification of environmental
contamination, as well as objective assessment of OM with respect to their
chemical contents
Laser Based Mid-Infrared Spectroscopic Imaging â Exploring a Novel Method for Application in Cancer Diagnosis
A number of biomedical studies have shown that mid-infrared spectroscopic images can provide
both morphological and biochemical information that can be used for the diagnosis of cancer. Whilst
this technique has shown great potential it has yet to be employed by the medical profession. By
replacing the conventional broadband thermal source employed in modern FTIR spectrometers with
high-brightness, broadly tuneable laser based sources (QCLs and OPGs) we aim to solve one of the
main obstacles to the transfer of this technology to the medical arena; namely poor signal to noise
ratios at high spatial resolutions and short image acquisition times. In this thesis we take the first
steps towards developing the optimum experimental configuration, the data processing algorithms
and the spectroscopic image contrast and enhancement methods needed to utilise these high
intensity laser based sources. We show that a QCL system is better suited to providing numerical
absorbance values (biochemical information) than an OPG system primarily due to the QCL pulse
stability. We also discuss practical protocols for the application of spectroscopic imaging to cancer
diagnosis and present our spectroscopic imaging results from our laser based spectroscopic imaging
experiments of oesophageal cancer tissue
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