Invasive alien species : a threat to sustainable livelihoods in the Pacific? : an assessment of the effects of Wasmannia auropunctata (little fire ant) and Achatina fulica (giant African snail) on rural livelihoods in the Solomon Islands : a dissertation presented in fulfilment of the requirements for the degree of Doctor of Philosophy in Development Studies, Massey University, Manawatū, New Zealand

Invasive alien species (IAS) are a global phenomenon and are recognised as a driver of environmental change which can affect the well-being of people in a multitude of ways. Despite this, the role of IAS in local livelihoods has received relatively little attention. Influencing all three of the sustainable development pillars (social, economic, environmental), IAS should be recognised as a significant development issue. But they are not. As such, IAS issues are new to many sectors and governments and therefore largely go unseen and un-actioned. Contemporary rural livelihoods in the Solomon Islands are heavily reliant on subsistence/semi-subsistence agriculture. Following a livelihoods’ framework developed for the Solomon Islands, this thesis explores the influence IAS have on rural livelihoods in this country. Using two qualitative case studies, Wasmannia auropunctata (little fire ant) and Achatina fulica (giant African snail), this study investigates how vulnerable/resilient rural livelihoods are to the effects of IAS and the implications IAS have for sustainable development in the Solomon Islands. The effects of IAS on rural livelihoods are complex and at times contradictory. W. auropunctata for the most part is not negatively affecting the dominant livelihood strategy (subsistence/semi-subsistence agriculture) practised in the Solomon Islands. While there are some social impacts associated with W. auropunctata, overall Solomon Island households can be considered resilient to this IAS. Achatina fulica is a different story. This species is negatively affecting the subsistence/semi-subsistence agricultural sector on which so many rural Solomon Island households depend. This has resulted in households implementing negative livelihood diversification measures as they fail to cope or adapt to the snails’ presence. Unlike for W. auropunctata, Solomon Island households have not demonstrated any resilience to A. fulica. Understanding how rural livelihoods are affected by various stressors and adverse events can help to design development policies and interventions geared towards building better lives for all people. This can only occur however, if the full range of shocks are recognised. To date, this is not the case for IAS, and as such, they are still a significant missing component of development policy

Colorimetric and spectroscopic analysis of mucus of Achatina sp terrestrial snails fed in differentiated diet

Foram estudados os efeitos da adição de plantas medicinais de princípios cicatrizantes (Centelha asiática, Papaína e Confrei) na ração controle de caracóis terrestres, para se avaliar a interferência destas plantas na composição do muco glicoprotéico. Foram utilizados 80 caracóis terrestres Achatina sp, baseados em um peso homogêneo (49 e 40 g e idade média de 10 e 19 meses para Achatina fulica e Achatina monochromatica, respectivamente). Os animais foram distribuídos aleatoriamente em oito grupos experimentais: controle Achatina fulica (FC) e Achatina monochromatica (MC), centelha asiática Achatina fulica (FCe) e Achatina monochromatica (MCe), papaína Achatina fulica (FPa) e Achatina monochromatica (MPa) e confrei Achatina fulica (FCo) e Achatina monochromatica (MCo). Água e ração foram fornecidos ad libitum. Ao final de 150 dias de tratamento, os animais foram submetidos à técnica de extração do muco glicoprotéico, por meio do estímulo manual da glândula podal, responsável pela secreção deste muco. Esta metodologia considerou o bem-estar dos animais, uma vez que os mesmos não foram sacrificados e retornaram ao seu sistema de criação. Os mucos foram analisados por meio de testes colorimétricos e espectroscópicos, que constataram alterações semelhantes, porém apresentaram variação significativa em sua composição glicoprotéica.The effects of adding medicinal plants with healing properties (Centelha asiatica, Papaína and Confrei) in the control diet of land snails were studied to evaluate the effect of these plants on the mucus glicoproteic composition. Eighty Achatina sp snails, based on a homogeneous weight (49 and 40 g and averaging 10 and 19 months of age for Achatina fulica and Achatina monochromatica, respectively). The animals were randomly allotted to eight experimental groups: Achatina fulica (FC) and Achatina monochromatica (MC) control, Asian Achatina fulica (FCe) and Achatina monochromatica (MCe) centelha, Achatina fulica (FPa) and Achatina monochromatica (MPa) papaína and Achatina fulica (FCo) and Achatina monochromatica (MCo) confrei. Ration and water were fed ad libitum. At the end of 150 days of treatment, the animals were submitted to the technique for extraction of mucus glicoproteic by manual stimulation of podal gland, responsible for the secretion of this mucus. This methodology considered the well-being of the animals since they were not slaughtered but returned to their creation system. The mucus was analyzed by means of color and spectroscopic tests, that indicated similar changes, but showed significant variation in their glicoproteic composition

Effect of Escherichia coli endotoxin on Archachatina marginata haemolymph coagulation system

The effect of _E. coli_ endotoxin on the heamolymph coagulation response of _Archachatina marginata_ was studied. Heamocyte Lysate(HL), Haemocyte Lysate Supernatant(HLS) and Haemocyte Lysate Debris (HLD) were exposed to _Escherichia coli_ endotoxin. Controls were prepared with endotoxin-free water(<0.025 EU/ml). The differential protein coagulation was estimated in each mixture. Fractions of the haemolymph exposed to endotoxin produced higher protein coagulates than endotoxin-free fractions when incubated at 37 °C for 1 h (p<0.05). The results showed significantly higher (p<0.05) concentrations of protein coagulated when HL/plasma mixture were used than when either fraction was used. At a ratio 1:1 of HL:Plasma, highest protein coagulation was recorded. This study revealed that maximum protein coagulation in response to endotoxin was elicited by a synergy between plasma and haemocyte lysate(HL). From this research haemolymph fractions of Archachatina marginata may provide an alternative test material for endotoxin in medical preparations in the future

Gastropod-derived haemocyte extracellular traps entrap metastrongyloid larval stages of Angiostrongylus vasorum, Aelurostrongylus abstrusus and Troglostrongylus brevior

Background: Phagocyte-derived extracellular traps (ETs) were recently demonstrated mainly in vertebrate hosts as an important effector mechanism against invading parasites. In the present study we aimed to characterize gastropod-derived invertebrate extracellular phagocyte trap (InEPT) formation in response to larval stages of important canine and feline metastrongyloid lungworms. Gastropod haemocytes were isolated from the slug species Arion lusitanicus and Limax maximus, and the snail Achatina fulica, and exposed to larval stages of Angiostrongylus vasorum, Aelurostrongylus abstrusus and Troglostrongylus brevior and investigated for gastropod-derived InEPT formation. Results: Phase contrast as well as scanning electron microscopy (SEM) analyses of lungworm larvae-exposed haemocytes revealed ET-like structures to be extruded by haemocytes thereby contacting and ensnaring the parasites. Co-localization studies of haemocyte-derived extracellular DNA with histones and myeloperoxidase in larvae-entrapping structures confirmed classical characteristics of ETs. In vivo exposure of slugs to A. vasorum larvae resulted in InEPTs being extruded from haemocytes in the slug mucous extrapallial space emphasizing the pivotal role of this effector mechanism against invasive larvae. Functional larval entrapment assays demonstrated that almost half of the haemocyte-exposed larvae were contacted or even immobilized by released InEPTs. Overall, as reported for mammalian-derived ETs, different types of InEPTs were here observed, i.e. aggregated, spread and diffused InEPTs. Conclusions: To our knowledge, this study represents the first report on metastrongyloid lungworm-triggered ETosis in gastropods thereby providing evidence of early mollusc host innate immune reactions against invading larvae. These findings will contribute to the better understanding on complex parasite-intermediate host interactions since different gastropod species bear different transmitting capacities for metastrongyloid infections

The giant African snail, Achatina fulica (Gastropoda: Achatinidae): Using bioclimatic models to identify South American areas susceptible to invasion

Fil: Vogler, Roberto Eugenio. División Zoología Invertebrados. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Beltramino, Ariel Anibal. División Zoología Invertebrados. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Sede, Mariano. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA. Facultad de Medicina. Universidad de Buenos Aires; ArgentinaFil: Gutiérrez Gregoric, Diego Eduardo. División Zoología Invertebrados. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Núñez, María Verónica. División Zoología Invertebrados. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Rumi, Alejandra. División Zoología Invertebrados. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin

Изменения сердечного ритма гигантской африканской улитки разного возраста

Исследована взаимосвязь между частотой сердечных сокращений и массой тела гигантских африканских улиток Achatina fulica разного возраста, начиная от вылупления до полового созревания. Был использован метод неинвазивной регистрации сердцебиени

Distribuição atual de Achatina fulica Bowdich, 1822 no Estado de São Paulo com registro de infestação por larvas de Aelurostrongylus abstrusus (Nematoda)

É apresentada a distribuição de Achatina fulica Bowdich, 1822 no Estado de São Paulo, Brasil. É fornecido o registro de A. fulica naturalmente infestada por larvas de Aelurostrongylus abstrusus (Railliet, 1898) (Nematoda: Metastrongylidae) no município de Guaratinguetá. Foi encontrada A. fulica portando larvas de Metastrongylidae sem importância médica e veterinária conhecida nos municípios de Carapicuíba, Embu Guaçu, Itapevi, São Caetano do Sul, São Paulo e Taboão da Serra.The currently known distribution range of Achatina fulica Bowdich, 1822, in the state of São Paulo, Brazil, is presented. The record of A. fulica naturally infested with Aelurostrongylus abstrusus larvae (Railliet, 1898) (Nematoda: Metastrongylidae) can be found in the city of Guaratinguetá. It was found A. fulica with Metastrongylidae larvae without known medical and veterinary importance in the cities of Carapicuíba, Embu-Guaçu, Itapevi, São Caetano do Sul, São Paulo and Taboão da Serra

Ocorrência do caramujo-africano (Achatina fulica) atacando cactáceas no estado do Ceará.

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The giant African snail, <i>Achatina fulica</i> (Gastropoda: Achatinidae): Using bioclimatic models to identify South American areas susceptible to invasion

The best way to reduce problems related to invasive species is by preventing introductions into potentially susceptible areas. The purpose of this study was to create distribution models for the invasive gastropod Achatina fulica Bowdich, 1822 in South America in order to evaluate its potential geographic distribution and identify areas at potential risk. This mollusc, considered one of the 100 world's worst invasive alien species, is the focus of intense concern due to its impact on agriculture, human health, and native fauna. We tested two commonly used ecological niche modeling methods: Genetic Algorithm for Rule-Set Prediction (GARP) and Maximum Entropy (MaxEnt). Models were run with occurrence points obtained from several sources, including the scientific literature, international databases, governmental reports and newspapers, WorldClim bioclimatic variables, and altitude. Models were evaluated with the threshold-independent Receiver Operating Characteristic (ROC) and Area Under the Curve (AUC). Both models had consistent performances with similar areas predicted as susceptible, including areas already affected and new potentially susceptible areas in both tropical and temperate regions of South America.Facultad de Ciencias Naturales y Muse