The effect of _E. coli_ endotoxin on the heamolymph coagulation response of _Archachatina marginata_ was studied. Heamocyte Lysate(HL), Haemocyte Lysate Supernatant(HLS) and Haemocyte Lysate Debris (HLD) were exposed to _Escherichia coli_ endotoxin. Controls were prepared with endotoxin-free water(<0.025 EU/ml). The differential protein coagulation was estimated in each mixture. Fractions of the haemolymph exposed to endotoxin produced higher protein coagulates than endotoxin-free fractions when incubated at 37 °C for 1 h (p<0.05). The results showed significantly higher (p<0.05) concentrations of protein coagulated when HL/plasma mixture were used than when either fraction was used. At a ratio 1:1 of HL:Plasma, highest protein coagulation was recorded. This study revealed that maximum protein coagulation in response to endotoxin was elicited by a synergy between plasma and haemocyte lysate(HL). From this research haemolymph fractions of Archachatina marginata may provide an alternative test material for endotoxin in medical preparations in the future
