Low-Noise Micro-Power Amplifiers for Biosignal Acquisition

There are many different types of biopotential signals, such as action potentials (APs), local field potentials (LFPs), electromyography (EMG), electrocardiogram (ECG), electroencephalogram (EEG), etc. Nerve action potentials play an important role for the analysis of human cognition, such as perception, memory, language, emotions, and motor control. EMGs provide vital information about the patients which allow clinicians to diagnose and treat many neuromuscular diseases, which could result in muscle paralysis, motor problems, etc. EEGs is critical in diagnosing epilepsy, sleep disorders, as well as brain tumors. Biopotential signals are very weak, which requires the biopotential amplifier to exhibit low input-referred noise. For example, EEGs have amplitudes from 1 μV [microvolt] to 100 μV [microvolt] with much of the energy in the sub-Hz [hertz] to 100 Hz [hertz] band. APs have amplitudes up to 500 μV [microvolt] with much of the energy in the 100 Hz [hertz] to 7 kHz [hertz] band. In wearable/implantable systems, the low-power operation of the biopotential amplifier is critical to avoid thermal damage to surrounding tissues, preserve long battery life, and enable wirelessly-delivered or harvested energy supply. For an ideal thermal-noise-limited amplifier, the amplifier power is inversely proportional to the input-referred noise of the amplifier. Therefore, there is a noise-power trade-off which must be well-balanced by the designers. In this work I propose novel amplifier topologies, which are able to significantly improve the noise-power efficiency by increasing the effective transconductance at a given current. In order to reject the DC offsets generated at the tissue-electrode interface, energy-efficient techniques are employed to create a low-frequency high-pass cutoff. The noise contribution of the high-pass cutoff circuitry is minimized by using power-efficient configurations, and optimizing the biasing and dimension of the devices. Sufficient common-mode rejection ratio (CMRR) and power supply rejection ratio (PSRR) are achieved to suppress common-mode interferences and power supply noises. Our design are fabricated in standard CMOS processes. The amplifiers’ performance are measured on the bench, and also demonstrated with biopotential recordings

Wearable System Based on Ultra-Thin Parylene C Tattoo Electrodes for EEG Recording

In an increasingly interconnected world, where electronic devices permeate every aspect of our lives, wearable systems aimed at monitoring physiological signals are rapidly taking over the sport and fitness domain, as well as biomedical fields such as rehabilitation and prosthetics. With the intent of providing a novel approach to the field, in this paper we discuss the development of a wearable system for the acquisition of EEG signals based on a portable, low-power custom PCB specifically designed to be used in combination with non-conventional ultra-conformable and imperceptible Parylene-C tattoo electrodes. The proposed system has been tested in a standard rest-state experiment, and its performance in terms of discrimination of two different states has been compared to that of a commercial wearable device for EEG signal acquisition (i.e., the Muse headset), showing comparable results. This first preliminary validation demonstrates the possibility of conveniently employing ultra-conformable tattoo-electrodes integrated portable systems for the unobtrusive acquisition of brain activity

Entwicklung einer berührungslosen EEG-Mütze mittels kapazitiver Elektroden

Non-contact capacitive electrodes for bioelectric diagnostics provide an interesting alternative to classical galvanically coupled electrodes. Such a low cost diagnostic system can be applied without preparation time and in mobile wireless environments. For even higher user comfort textile capacitive electrodes are preferable. In this work, a comprehensive model for the electronic noise properties and frequency dependent responses of PCB-based, as well as textile non-contact capacitive electrodes, is presented. A thorough study of the influence of the electrical components on the resulting noise properties of these electrodes, is provided by independently measuring the corresponding noise spectra. The most important low frequency noise source of capacitive electrode is the necessary high input bias resistance. By comparing the noise measurements with the theoretical noise model of the electrode, it is concluded that the surface of the electrode contributes to an additional 1/f-power noise. It is also found that the highest possible coupling capacitance is most favorable for low noise behavior. Therefore, we implemented electrodes with electrically conducting fabric surfaces. With these electrodes, it is possible to enlarge the surface of the electrode while simultaneously maintaining a small distance between the body and the electrode over the whole surface area, thus maximizing the capacitance. We also show that the use of textile capacitive electrodes, reduces the noise considerably. Furthermore, this thesis describes the construction of a capacitive non-contact textile electroencephalography measuring hat (cEEG hat) with seven measuring channels. This hat benefits from the low noise characteristics of the integrated developed textile capacitive electrodes. The measured noise spectrum of this cEEG hat shows low noise characteristics at low frequencies. This fulfills many requirements for measuring brain signals. The implemented cEEG hat is comfortable to wear during very long measurements and even during sleep periods. In contrast to common methods, the cEEG hat provides a possibility of measuring EEG signal during sleep outside laboratories and in the comfort of home. EEG sleep measurements shown in this work, are recorded inside a normal apartment. The possibility of brain computer interface application is also shown by measuring steady state visually evoked potentials (SSVEP) at different frequencies.Berührungslose, kapazitive Elektroden für bioelekrische Untersuchungen stellen eine interessante Alternative zu klassischen galvanisch gekoppelten Elektroden dar. Ein solches preisgünstiges Diagnosesystem kann ohne lange Vorbereitungszeit und in mobilen Umgebungen eingesetzt werden. Für gesteigerten Tragekomfort sind textile Elektroden von Vorteil. In dieser Arbeit wird eine umfassende Beschreibung der elektronischen Rauscheigenschaften und des frequenzabhängigen Verhaltens von sowohl platinenbasierten, als auch textilen kapazitiven Elektroden vorgestellt. Die Einflüsse aller elektronischen Komponenten auf die resultierenden Rauscheigenschaften werden durch Messungen der entsprechenden Rauschspektren untersucht. Die wichtigste niederfrequente Rauschquelle kapazitiver Elektroden stellt der notwendige und zugleich hohe Bias-Eingangswiderstand dar. Durch Vergleich der gemessenen Rauschspektren mit dem theoretischen Modell wird die Oberfläche der Elektroden als eine zusätzliche 1/f-Rauschquelle identifiziert. Dabei ist die größtmögliche Kopplungskapazität vorteilhaft für ein niedriges Rauschen. Deshalb setzen wir im Folgenden Elektroden aus elektrisch leitfähigen Textilien ein. Mit diesen Elektroden ist es möglich, die Oberfläche der Elektrode unter gleichzeitiger Beibehaltung eines kleinen Abstandes zum Körper zu vergrößern. Dies maximiert wiederum die Kapazität. Wir zeigen zudem, dass die Verwendung textiler kapazitiver Elektroden die Rauscheigenschaften deutlich verbessert. Desweiteren wird in dieser Arbeit die Konstruktion eines kapazitiven, berührungslosen EEG-Helmes (cEEG-Mütze) mit sieben Kanälen beschrieben. Dieser Helm profitiert von den guten Rauscheigenschaften der zuvor entwickelten und hier integrierten textilen Elektroden. Die gemessenen Rauschspektren zeigen ein niedriges Rauschen im unteren Frequenzbereich. Dies erfüllt viele Voraussetzungen für die Messung von Gehirnsignalen. Die erstellte cEEG-Mütze lässt sich während langer Messzeiten und Schlafperioden angenehm tragen. Im Gegensatz zu herkömmlichen Methoden ermöglicht sie Messungen außerhalb von Laboratorien und im gewohnten Umfeld. Alle in dieser Arbeit gezeigten Schlafmessungen wurden in einer normalen Wohnung aufgezeichnet. Außerdem wird die Einsatzmöglichkeit für sogenannte ”Gehirn-Computer-Schnittstellen” anhand der Messung von ”steady state visually evoked potentials” (SSVEP) Signalen bei verschiedenen Frequenzen demonstriert

Amplifiers in Biomedical Engineering: A Review from Application Perspectives

Continuous monitoring and treatment of various diseases with biomedical technologies and wearable electronics has become significantly important. The healthcare area is an important, evolving field that, among other things, requires electronic and micro-electromechanical technologies. Designed circuits and smart devices can lead to reduced hospitalization time and hospitals equipped with high-quality equipment. Some of these devices can also be implanted inside the body. Recently, various implanted electronic devices for monitoring and diagnosing diseases have been presented. These instruments require communication links through wireless technologies. In the transmitters of these devices, power amplifiers are the most important components and their performance plays important roles. This paper is devoted to collecting and providing a comprehensive review on the various designed implanted amplifiers for advanced biomedical applications. The reported amplifiers vary with respect to the class/type of amplifier, implemented CMOS technology, frequency band, output power, and the overall efficiency of the designs. The purpose of the authors is to provide a general view of the available solutions, and any researcher can obtain suitable circuit designs that can be selected for their problem by reading this survey

Biomimetic Sonar for Electrical Activation of the Auditory Pathway

Relying on the mechanism of bat’s echolocation system, a bioinspired electronic device has been developed to investigate the cortical activity of mammals in response to auditory sensorial stimuli. By means of implanted electrodes, acoustical information about the external environment generated by a biomimetic system and converted in electrical signals was delivered to anatomically selected structures of the auditory pathway. Electrocorticographic recordings showed that cerebral activity response is highly dependent on the information carried out by ultrasounds and is frequency-locked with the signal repetition rate. Frequency analysis reveals that delta and beta rhythm content increases, suggesting that sensorial information is successfully transferred and integrated. In addition, principal component analysis highlights how all the stimuli generate patterns of neural activity which can be clearly classified. The results show that brain response is modulated by echo signal features suggesting that spatial information sent by biomimetic sonar is efficiently interpreted and encoded by the auditory system. Consequently, these results give new perspective in artificial environmental perception, which could be used for developing new techniques useful in treating pathological conditions or influencing our perception of the surroundings

Wireless system for the measurement of bioelectric signals using capacitive electrodes

Català: Aquest projecte mostra el disseny i la implementació d'un sistema d'adquisició de senyals bioelèctriques mitjançant elèctrodes capacitius. El sistema, inalàmbric i a bateria, envia les dades a un ordinador per a la seva visualització o processament.Castellano: Este proyecto muestra el diseño y la implementación de un sistema de adquisición de señales bioeléctricas mediante electrodos capacitivos. El sistema, inalámbrico y a batería, envía los datos a un ordenador para su visualización o procesamiento.English: This project shows the design and implementation of an acquisition system for the measurement of bioelectric signals by means of capacitive electrodes. The system, which is wireless and battery powered, sends the data to a computer for its visualizations or processing

Carbon Fiber Electrode Arrays for Cortical and Peripheral Neural Interfaces

Neural interfaces create a connection between neural structures in the body and external electronic devices. Brain-machine interfaces and bioelectric medicine therapies rely on the seamless integration of neural interfaces with the brain, nerves, or spinal cord. However, conventional neural interfaces cannot meet the demands of high channel count, signal fidelity, and signal longevity that these applications require. I investigated the damage resulting from conventional Utah arrays after multiple years of implantation in the cortex of a non-human primate as a possible explanation for these limitations. The neuron density around the electrode shanks was compared to the neuron density of nearby healthy tissue, finding a 73% loss in density around the electrodes. The explanted arrays were imaged and characterized for degradation. Coating cracks, tip breakage, and parylene cracks were the most common degradation type. A significantly higher number of tip breakage and coating crack occurrences were found on the edges of the arrays as compared to the middle. In this work, I made clear the need for a minimally damaging alternative to the Utah electrode array. Neural interfaces composed of carbon fiber electrodes, with a diameter of 6.8 microns, could enable a seamless integration with the body. Previous work resulted in an array of individuated carbon fiber electrodes that reliably recorded high signal-to-noise ratio neural signals from the brain for months. However, the carbon fiber arrays were limited by only 30% of the electrodes recording neural signals, despite inducing minimal inflammation. Additionally, it was relatively unknown if carbon fibers would make suitable long-term peripheral neural interfaces. Here, I illustrate the potential of carbon fiber electrodes to meet the needs of a variety of neural applications. First, I optimized state-of-the-art carbon fiber electrodes to reliably record single unit electrophysiology from the brain. By analyzing the previous manufacturing process, the cause of the low recording yield of the carbon fiber arrays was identified as the consistency of the electrode tip. A novel laser cutting technique was developed to produce a consistent carbon fiber tip geometry, resulting in a near tripling of recording yield of high amplitude chronic neural signals. The longevity of the carbon fiber arrays was also addressed. The conventional polymer coating was compared against platinum iridium coating and an oxygen plasma treatment, both of which outperformed the polymer coating. In this work, I customized carbon fiber electrodes for reliable, long-term neural recording. Secondly, I translated the carbon fiber technology from the brain to the periphery in an architecture appropriate for chronic implantation. The insertion of carbon fibers into the stiffer structures in the periphery is enabled by sharpening the carbon fibers. The sharpening process combines a butane flame to sharpen the fibers with a water bath to protect the base of the array. Sharpened carbon fiber arrays recorded electrophysiology from the rat vagus nerve and feline dorsal root ganglia, both structures being important targets for bioelectric medicine therapies. The durability of carbon fibers was also displayed when partially embedded carbon fibers in medical-grade silicone withstood thousands of repeated bends without fracture. This work showed that carbon fibers have the electrical and structural properties necessary for chronic application. Overall, this work highlights the vast potential of carbon fiber electrodes. Through this thesis, future brain-machine interfaces and bioelectric medicine therapies may utilize arrays of sub-cellular electrodes such as carbon fibers in medical applications.PHDBiomedical EngineeringUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/169982/1/elissajw_1.pd

New Techniques for Multi-Channel Biosignal Acquisition and Low-Power, Low-Resistance-Measurement Systems

Dense electrical recording of biosignals has been developed to provide spatial resolution and precise temporal information for health monitoring, diagnostics, and clinical research. However, more electrodes require more wires, and wiring density quickly becomes a limiting factor. To break this bottleneck, we proposed a frequency-division multiplexing (FDM) based architecture for multi-channel acquisition systems. In this final exam, I present two applications that make use of this FDM technique. The first is an FDM-based multi-channel electromyography (EMG) acquisition system, which demonstrates that the FDM system not only reduces wire count, but also mitigates the effect of low frequency motion artifacts and 50/60 Hz mains interference introduced in the wire. An FDM-based four-channel EMG recording is demonstrated, while carrying all channels over a 3-wire interface, and the system achieves an attenuation of low-frequency cable motion artifacts by 15X an! d 60Hz mains noise coupled in the cable by 62X. A second application that forms the basis of my current research effort is an FDM-based neural recording system with multiple graphene active electrodes. We demonstrated a two-channel system including graphene FET electrodes, a custom integrated circuit (IC) analog front-end (AFE), and digital demodulation. In related multi-channel sensor work, a growing need for ultra-low-power sensors has driven continuous advancement in read-out circuits for temperature, humidity, and pressure. IC-integrated Wheatstone bridges, commonly used, are efficient for large sensor resistance (5k-500kohm), but measuring small resistance (30,000x smaller nominal sensor resistance

Power Efficient Data Compression Hardware for Wearable and Wireless Biomedical Sensing Devices

This thesis aims to verify a possible benefit lossless data compression and reduction techniques can bring to a wearable and wireless biomedical device, which is anticipated to be system power saving. A wireless transceiver is one of the main contributors to the system power of a wireless biomedical sensing device, and reducing the data transmitted by the transceiver with a minimum hardware cost can therefore help to save the power. This thesis is going to investigate the impact of the data compression and reduction on the system power of a wearable and wireless biomedical device and trying to find a proper compression technique that can achieve power saving of the device. The thesis first examines some widely used lossy and lossless data compression and reduction techniques for biomedical data, especially EEG data. Then it introduces a novel lossless biomedical data compression technique designed for this research called Log2 sub-band encoding. The thesis then moves on to the biomedical data compression evaluation of the Log2 sub-band encoding and an existing 2-stage technique consisting of the DPCM and the Huffman encoding. The next part of this thesis explores the signal classification potential of the Log2 sub-band encoding. It was found that some of the signal features extracted as a by-product during the Log2 sub-band encoding process could be used to detect certain signal events like epileptic seizures, with a proper method. The final section of the thesis focuses on the power analysis of the hardware implementation of two compression techniques referred to earlier, as well as the system power analysis. The results show that the Log2 sub-band is comparable and even superior to the 2-stage technique in terms of data compression and power performance. The system power requirement of an EEG signal recorder that has the Log2 sub-band implemented is significantly reduced

Electrical characterization of electronic interfaces to communicate with electrogenic cells in vitro

This document reflects the work done in the development of new electronic biocompatible and biodegradable materials that allow the construction of implantable transistors in living beings, able to communicate with nerve cells (neurons). These electronic components will be able to repair parts of the nervous tissue and restore communication between the nerve tissues, e.g. repair spinal cord injury. The motivation of this work arises from the need to develop new instruments that help us understand fundamental aspects of biology, in particular on how nerve cells interact with each other and respond to electrical and biochemical stimuli. For this purpose, a search was performed involving various aspects, (i) applying biocompatible materials with electrical properties for constructing transistors, (ii) the study of electrochemical processes occurring at the interface between the transducer and the cell membrane, and (iii ) acquisition and stimulation of bioelectric signals. During investigation were characterized electronic components capable of operating in complex electrolyte media, in particular, capacitors of the type Metal-Insulator- Semiconductor (MIS); electrical noise measurement techniques and spectral analysis for identifying bioelectrical signals were carried out, and were obtained and characterized the bioelectric signals produced by cells when subjected to electrical and extracellular biochemical stimulation. These technical measures prove the possibility to monitor the cells adhesion and their health state, their bioelectric activity and the effect caused by the addition of extracellular stimuli.O presente documento reflete o trabalho realizado no âmbito do desenvolvimento de novos materiais eletrónicos biocompatíveis e biodegradáveis que veio permitir a construção de transístores implantáveis em seres vivos e capazes de comunicar com células nervosas (neurónios). Estes componentes eletrónicos serão capazes de reparar partes do tecido nervoso e restaurar a comunicação entre tecidos nervosos, por exemplo reparar lesões na medula espinal. A motivação deste trabalho surge com a necessidade de desenvolver novos instrumentos que nos ajudam a perceber aspetos fundamentais da biologia, nomeadamente sobre como as células nervosas interatuam umas com as outras e respondem a estímulos elétricos e bioquímicos. Para tal, foi efetuada uma pesquisa que envolve vários aspetos, (i) a aplicação de materiais biocompatíveis com propriedades elétricas para a construção de transístores, (ii) estudo de processos eletroquímicos que ocorrem na interface entre o transdutor e a membrana celular, e (iii) a aquisição e estímulo de sinais bioelétricos. No decorrer da investigação, foram caracterizados componentes eletrónicos capazes de operar em meios eletrolíticos complexos, nomeadamente, condensadores do tipo Metal-Isolador-Semicondutor (MIS); foram realizadas técnicas de medidas de ruído elétrico e análise espectral para a identificação de sinais bioelétricos, bem como foram obtidos e caracterizados os sinais bioelétricos produzidos por células quando submetidas a estímulos elétricos e bioquímicos extracelulares. Estas técnicas de medidas vêm comprovar a possibilidade de monitorizar a adesão das células e o seu estado de saúde, a sua atividade bioelétrica e o efeito provocado pela adição de estímulos extracelulares.Universidade do Algarve, Faculdade de Ciências e Tecnologi