26 research outputs found
Natural antisense transcripts regulate the neuronal stress response and excitability
Neurons regulate ionic fluxes across their plasma membrane to maintain their excitable properties under varying environmental conditions. However, the mechanisms that regulate ion channels abundance remain poorly understood. Here we show that pickpocket 29 (ppk29), a gene that encodes a Drosophila degenerin/epithelial sodium channel (DEG/ENaC), regulates neuronal excitability via a protein-independent mechanism. We demonstrate that the mRNA 3′UTR of ppk29 affects neuronal firing rates and associated heat-induced seizures by acting as a natural antisense transcript (NAT) that regulates the neuronal mRNA levels of seizure (sei), the Drosophila homolog of the human Ether-à-go-go Related Gene (hERG) potassium channel. We find that the regulatory impact of ppk29 mRNA on sei is independent of the sodium channel it encodes. Thus, our studies reveal a novel mRNA dependent mechanism for the regulation of neuronal excitability that is independent of protein-coding capacity. DOI: http://dx.doi.org/10.7554/eLife.01849.00
Genome-wide screen for modifiers of Na<sup>+</sup>/K<sup>+</sup> ATPase alleles identifies critical genetic loci
Background: Mutations affecting the Na+/ K+ ATPase (a.k.a. the sodium-potassium pump) genes cause conditional locomotor phenotypes in flies and three distinct complex neurological diseases in humans. More than 50 mutations have been identified affecting the human ATP1A2 and ATP1A3 genes that are known to cause rapid-onset Dystonia Parkinsonism, familial hemiplegic migraine, alternating hemiplegia of childhood, and variants of familial hemiplegic migraine with neurological complications including seizures and various mood disorders. In flies, mutations affecting the ATPalpha gene have dramatic phenotypes including altered longevity, neural dysfunction, neurodegeneration, myodegeneration, and striking locomotor impairment. Locomotor defects can manifest as conditional bang-sensitive (BS) or temperature-sensitive (TS) paralysis: phenotypes well-suited for genetic screening. Results: We performed a genome-wide deficiency screen using three distinct missense alleles of ATPalpha and conditional locomotor function assays to identify novel modifier loci. A secondary screen confirmed allele-specificity of the interactions and many of the interactions were mapped to single genes and subsequently validated. We successfully identified 64 modifier loci and used classical mutations and RNAi to confirm 50 single gene interactions. The genes identified include those with known function, several with unknown function or that were otherwise uncharacterized, and many loci with no described association with locomotor or Na+/K+ ATPase function. Conclusions: We used an unbiased genome-wide screen to find regions of the genome containing elements important for genetic modulation of ATPalpha dysfunction. We have identified many critical regions and narrowed several of these to single genes. These data demonstrate there are many loci capable of modifying ATPalpha dysfunction, which may provide the basis for modifying migraine, locomotor and seizure dysfunction in animals
05. 2014 IMSAloquium Student Investigation Showcase
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2014 IMSAloquium, Student Investigation Showcase
The ability to work with professionals is a life-changing experience for our students. Working with world-class scholars and advisors, students have contributed to advances in a variety of fields from science, technology, engineering and mathematics, to the performing arts and history.https://digitalcommons.imsa.edu/archives_sir/1006/thumbnail.jp
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Study of Cyclophilin A Function in Models of Amyotrophic Lateral Sclerosis
Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease targeting preferentially motor neurons. Cyclophilin A (CypA) was identified as a hallmark of disease in mutant SOD1 (mSOD1) animal models of familial ALS (fALS) at a presymptomatic stage, and in sporadic (sALS) patients (Massignan 2007; Nardo 2011). Moreover, CypA was enriched in the spinal cord aggregates of mSOD1 mice and sALS patients (Basso 2009). CypA is an ubiquitous protein with multiple functions relevant to the CNS, where it is abundantly expressed.
Insights into CypA function in ALS were provided via a proteomic analysis of its interacting proteins, that functionally associated CypA with different proteins networks. In particular, it extensively binds proteins regulating RNA metabolism, including several hnRNPs and TDP-43, a major disease protein in ALS. TDP-43 and CypA interact in the nucleus, in an RNA-dependent way. CypA has a key role in the stabilization of TDP-43/hnRNP A2/B1 interaction, and TDP-43-mediated HDAC6 expression regulation, properties impaired in TDP-43 ALS-mutants, possibly because of a loss-of-interaction with CypA. CypA interacts also with mSOD1, suggesting a gain-of-interaction specifically linked to fALS. Mice expressing mSOD1 and lacking CypA show increased levels of insoluble mSOD1 and hyperphosphorylated TDP-43 in the spinal cord at the onset.
This thesis work shows that CypA has a protective role in ALS: as a chaperone (for mSOD1) and in maintenance of multi-protein (TDP-43/hnRNPs) complex stability. Regardless the cause of the disease, mSOD1 or alterations in TDP-43, the interaction with CypA is impaired and it is cosequestered in proteinaceous aggregates, altering its protective activities. The net effect is the formation of pathological inclusions that may lead to a compromised RNA metabolism. CypA being a key interacting partner of both mSOD1 and TDP-43 can represent the "missing link" of these two patho-mechanisms in ALS and an interesting target for therapeutic interventions
Current Frontiers and Perspectives in Cell Biology
A numerous internationally renowned authors in the pages of this book present the views of the fields of cell biology and their own research results or review of current knowledge. Chapters are divided into five sections that are dedicated to cell structures and functions, genetic material, regulatory mechanisms, cellular biomedicine and new methods in cell biology. Multidisciplinary and often quite versatile approach by many authors have imposed restrictions of this classification, so it is certain that many chapters could belong to the other sections of this book. The current frontiers, on the manner in which they described in the book, can be a good inspiration to many readers for further improving, and perspectives which are highlighted can be seen in many areas of fundamental biology, biomedicine, biotechnology and other applications of knowledge of cell biology. The book will be very useful for beginners to gain insight into new area, as well as experts to find new facts and expanding horizons
Activation of the pro-resolving receptor Fpr2 attenuates inflammatory microglial activation
Poster number: P-T099
Theme: Neurodegenerative disorders & ageing
Activation of the pro-resolving receptor Fpr2 reverses inflammatory microglial activation
Authors: Edward S Wickstead - Life Science & Technology University of Westminster/Queen Mary University of London
Inflammation is a major contributor to many neurodegenerative disease (Heneka et al. 2015). Microglia, as the resident immune cells of the brain and spinal cord, provide the first line of immunological defence, but can become deleterious when chronically activated, triggering extensive neuronal damage (Cunningham, 2013). Dampening or even reversing this activation may provide neuronal protection against chronic inflammatory damage. The aim of this study was to determine whether lipopolysaccharide (LPS)-induced inflammation could be abrogated through activation of the receptor Fpr2, known to play an important role in peripheral inflammatory resolution. Immortalised murine microglia (BV2 cell line) were stimulated with LPS (50ng/ml) for 1 hour prior to the treatment with one of two Fpr2 ligands, either Cpd43 or Quin-C1 (both 100nM), and production of nitric oxide (NO), tumour necrosis factor alpha (TNFα) and interleukin-10 (IL-10)
were monitored after 24h and 48h. Treatment with either Fpr2 ligand significantly suppressed LPS-induced production of NO or TNFα after both 24h and 48h exposure, moreover Fpr2 ligand treatment significantly enhanced production of IL-10 48h post-LPS treatment. As we have previously shown Fpr2 to be coupled to a number of intracellular signaling pathways (Cooray et al. 2013), we investigated potential signaling
responses. Western blot analysis revealed no activation of ERK1/2, but identified a rapid and potent activation of p38 MAP kinase in BV2 microglia following stimulation with Fpr2 ligands. Together, these data indicate the possibility of exploiting immunomodulatory strategies for the treatment of neurological diseases, and highlight in particular the important potential of resolution mechanisms as novel therapeutic targets in neuroinflammation.
References
Cooray SN et al. (2013). Proc Natl Acad Sci U S A 110: 18232-7.
Cunningham C (2013). Glia 61: 71-90.
Heneka MT et al. (2015). Lancet Neurol 14: 388-40
Novel Approaches to Studying the Effects of Cis-Regulatory Variants in the Central Nervous System
For decades, studies of the genetic basis of disease have focused on rare coding mutations that disrupt protein function, leading to the identification of hundreds of genes underlying Mendelian diseases. However, many complex diseases are non-Mendelian, and less than 2% of the genome is coding. It is now clear that non-coding variants contribute to disease susceptibility, but the precise underlying mechanisms are generally unknown. Cis-regulatory elements (CREs) are transcription factor (TF)-bound genomic regions that regulate gene expression, and variants within CREs can therefore modify gene expression. The putative locations of CREs in a variety of cell types have been identified through genome-wide assays of TF binding and epigenomic signatures, providing a starting point for probing the effects of cis-regulatory variants. Unlike coding mutations, which can be interpreted based on the genetic code, the functional consequence of any given cis-regulatory variant is difficult to predict even at the molecular level. Therefore, a major bottleneck lies in interpreting the functional significance of these variants.
In the present work, I study the effects of cis-regulatory variants in the central nervous system (CNS), specifically in retina and brain. The retina is composed of well-characterized neuronal cell types and an extensively studied transcriptional network, while the brain is the center of human cognition and a target of devastating neuropsychiatric diseases. First, I take advantage of the genetic diversity between two distantly related mouse strains to describe the relationship between cis-regulatory variants and differences in retinal gene expression. I identify cis- and trans-regulatory effects, as well as parent-of-origin effects. Second, I develop a new technology based on an existing massively parallel reporter assay, CRE-seq, to enable the functional study of long CREs in the CNS in vivo for the first time. I demonstrate the ability of this approach to measure tissue-specific cis-regulatory activity in the brain and to pinpoint DNA bases critical for activity. Finally, I conduct a detailed mechanistic study of a non-coding region containing variants associated with both human cognitive performance and bipolar disorder. This last study illustrates the complexities and challenges of establishing the causal role of non-coding variants in disease
A Drosophila behavioral mutant, down and out (dao), is defective in an essential regulator of Erg potassium channels
To signal properly, excitable cells must establish and maintain the correct balance of various types of ion channels that increase or decrease membrane excitability. The mechanisms by which this balance is regulated remain largely unknown. Here, we describe a regulatory mechanism uncovered by a Drosophila behavioral mutant, down and out (dao). At elevated temperatures, dao loss-of-function mutants exhibit seizures associated with spontaneous bursts of neural activity. This phenotype closely resembles that of seizure mutations, which impair activity of ether-a-go-go-related gene (Erg)-type potassium channels. Conversely, neural over-expression of wild-type Dao confers dominant temperature-sensitive paralysis with kinetics reminiscent of paralytic sodium-channel mutants. The over-expression phenotype of dao is suppressed in a seizure mutant background, suggesting that Dao acts by an effect on Erg channels. In support of this hypothesis, functional expression of Erg channels in a heterologous system is dependent on the presence of Dao. These results indicate that Dao has an important role in establishing the proper level of neuronal membrane excitability by regulating functional expression of Erg channels