Experimental investigation of laminar turbulent intermittency in pipe flow

In shear flows turbulence first occurs in the form of localized structures (puffs/spots) surrounded by laminar fluid. We here investigate such spatially intermittent flows in a pipe experiment showing that turbulent puffs have a well defined interaction distance, which sets the minimum spacing of puffs as well as the maximum observable turbulent fraction. Two methodologies are employed here. Starting from a laminar flow puffs can be created by locally injecting a jet of fluid through the pipe wall. When the perturbation is applied periodically at low frequencies, as expected, a regular sequence of puffs is observed where the puff spacing is given by the ratio of the mean flow speed to the perturbation frequency. On the other hand, at large frequencies puffs are found to interact and annihilate each other. Varying the perturbation frequency an interaction distance can be determined. In the second set of experiments, the Reynolds number is reduced suddenly from fully developed turbulence to the intermittent regime.The resulting flow reorganizes itself to a sequence of constant size puffs which, unlike in Couette and Taylor Couette flow are randomly spaced. The minimum distance between the turbulent patches is identical to the puff interaction length. The puff interaction length is found to be in excellent agreement with the wavelength of regular stripe and spiral patterns in plane Couette and Taylor-Couette flow. We propose that the same interaction mechanism is present in these flows

Calcium puffs are generic InsP₃-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses

Elementary Ca2+ signals, such as "Ca2+ puffs", which arise from the activation of inositol 1,4,5-trisphosphate receptors, are building blocks for local and global Ca2+ signalling. We characterized Ca2+ puffs in six cell types that expressed differing ratios of the three inositol 1,4,5-trisphosphate receptor isoforms. The amplitudes, spatial spreads and kinetics of the events were similar in each of the cell types. The resemblance of Ca2+ puffs in these cell types suggests that they are a generic elementary Ca2+ signal and, furthermore, that the different inositol 1,4,5-trisphosphate isoforms are functionally redundant at the level of subcellular Ca2+ signalling. Hormonal stimulation of SH-SY5Y neuroblastoma cells and HeLa cells for several hours downregulated inositol 1,4,5-trisphosphate expression and concomitantly altered the properties of the Ca2+ puffs. The amplitude and duration of Ca2+ puffs were substantially reduced. In addition, the number of Ca2+ puff sites active during the onset of a Ca2+ wave declined. The consequence of the changes in Ca2+ puff properties was that cells displayed a lower propensity to trigger regenerative Ca2+ waves. Therefore, Ca2+ puffs underlie inositol 1,4,5-trisphosphate signalling in diverse cell types and are focal points for regulation of cellular responses

An Experimental Study of Momentum-Driven Unsteady Jets

Jets are seen commonly in nature and engineering and can be broadly classified into steady and unsteady jets. The study of unsteady jets has received little attention when compared to its steady counterpart. A type of unsteady jet is the turbulent puff which is a momentum driven jet in which fluid is ejected from a jet orifice intermittently. Common examples of turbulent puff like jets are coughs and volcanoes. The studies on momentum driven unsteady jets have primarily focused on single ejection events (single puffs) where an instantaneous supply of momentum drives the source fluid downstream of a nozzle. This work focuses on dual puffs in which two volumes of fluid (dual puffs), separated by a time ∆p are ejected from a jet orifice into ambient. An experimental framework to study such dual puffs with varying separation and ejected volume was built. The dual puffs studied were compared with both steady jets and single puffs. The mean velocity of these flow fields were measured using hot-wire anemometry. Complementary flow visualizations were also carried out. Dual puffs with ∆p = 0.3 s, 0.5 s, 0.7 s ans 1.79 s were considered. It was determined that for short time separations ∆pVj/d = 430, the dual puffs persisted for longer distances when compared to a single puff or dual puffs with larger time separation (∆pVj/d = 1540). Here Vj is the maximum jet exit velocity and the d the diameter of the jet orifice. However, at large time separation (∆pVj/d = 1540) the dual puffs expanded considerably more rapidly than dual puffs with smaller separation or single puffs. This indicates that the dual puff studied can be classified into two categories based on the time separation between the puffs. These observations are of an integral nature and a more detailed analysis of the flow field using advanced techniques as particle image velocimetry (PIV) are recommended to establish the precise flow physics leading to this behavior

Effects of structural and textural properties of brittle cereal foams on mechanisms of oral breakdown and in vitro starch digestibility

This study was jointly funded by the Academy of Finland (Academy Professorship, Kaisa Poutanen - grant number 131460) and by the Raisio Plc's Research Foundation (personal grant to Syed Ariful Alam, grant decisions of 2016).Structural and textural properties as well as the dietary fibre content of solid cereal foams influence the oral breakdown of structure, bolus formation and digestibility. The aimof this studywas to investigate howstructural differences of solid cereal foams (puffs vs. flakes) affect in vivo chewing and in vitro starch digestion. Four extruded puffs and flakes were produced from endosperm rye flour by extrusion processing without or with 10% rye bran (RB) addition. Extruded puffs and flakes were masticated by fifteen healthy females and the process was monitored using electromyography. Extruded puffs were more porous than flakes (97% vs 35%). The two productswere also significantly different (p b 0.05) in their structural and textural properties such as expansion, hardness, density and crispiness. A negative correlation was observed between hardness and crispiness index (p b 0.05, r=−0.950) and density and porosity (p b 0.05, r=−0.964). Addition of 10% RB had a significant effect on structural, textural and mastication properties both for puffs and flakes. Mastication of puffs required less total work than flakes (204 vs. 456%) and theywere degraded to smaller particles than flakes during mastication. Irrespectively of the considerable differences in structure, texture and oral disintegration process, no significant (p b 0.05) differenceswere observed between puffs and flakes (86.4 vs. 85.1) in terms of starch hydrolysis index. RB addition increased the hydrolysis index of puffs and flakes to 89.7 and 94.5, respectively, which was probably attributable to the increased number of particles in the bolus.Peer reviewe

A phenomenological cluster-based model of Ca2+ waves and oscillations for Inositol 1,4,5-trisphosphate receptor (IP3R) channels

Clusters of IP3 receptor channels in the membranes of the endoplasmic reticulum (ER) of many non-excitable cells release calcium ions in a cooperative manner giving rise to dynamical patterns such as Ca2+ puffs, waves, and oscillations that occur on multiple spatial and temporal scales. We introduce a minimal yet descriptive reaction-diffusion model of IP3 receptors for a saturating concentration of IP3 using a principled reduction of a detailed Markov chain description of individual channels. A dynamical systems analysis reveals the possibility of excitable, bistable and oscillatory dynamics of this model that correspond to three types of observed patterns of calcium release -- puffs, waves, and oscillations respectively. We explain the emergence of these patterns via a bifurcation analysis of a coupled two-cluster model, compute the phase diagram and quantify the speed of the waves and period of oscillations in terms of system parameters. We connect the termination of large-scale Ca2+ release events to IP3 unbinding or stochasticity.Comment: 18 pages, 10 figure

The Vegetarian Magazine January 1905

Periodical discussing the vegetarian lifestyle and recipes. Includes the regular column The Dining Room. Recipes include puffs, whole wheat gems, corn mush puffs, corn mush gems, and corn meal mush.https://knowledge.e.southern.edu/foodiesguide-1890/1067/thumbnail.jp

Transition to turbulence in pulsating pipe flow

Fluid flows in nature and applications are frequently subject to periodic velocity modulations. Surprisingly, even for the generic case of flow through a straight pipe, there is little consensus regarding the influence of pulsation on the transition threshold to turbulence: while most studies predict a monotonically increasing threshold with pulsation frequency (i.e. Womersley number, $\alpha$), others observe a decreasing threshold for identical parameters and only observe an increasing threshold at low $\alpha$. In the present study we apply recent advances in the understanding of transition in steady shear flows to pulsating pipe flow. For moderate pulsation amplitudes we find that the first instability encountered is subcritical (i.e. requiring finite amplitude disturbances) and gives rise to localized patches of turbulence ("puffs") analogous to steady pipe flow. By monitoring the impact of pulsation on the lifetime of turbulence we map the onset of turbulence in parameter space. Transition in pulsatile flow can be separated into three regimes. At small Womersley numbers the dynamics are dominated by the decay turbulence suffers during the slower part of the cycle and hence transition is delayed significantly. As shown in this regime thresholds closely agree with estimates based on a quasi steady flow assumption only taking puff decay rates into account. The transition point predicted in the zero $\alpha$ limit equals to the critical point for steady pipe flow offset by the oscillation Reynolds number. In the high frequency limit puff lifetimes are identical to those in steady pipe flow and hence the transition threshold appears to be unaffected by flow pulsation. In the intermediate frequency regime the transition threshold sharply drops (with increasing $\alpha$) from the decay dominated (quasi steady) threshold to the steady pipe flow level

Dense packing crystal structures of physical tetrahedra

We present a method for discovering dense packings of general convex hard particles and apply it to study the dense packing behavior of a one-parameter family of particles with tetrahedral symmetry representing a deformation of the ideal mathematical tetrahedron into a less ideal, physical, tetrahedron and all the way to the sphere. Thus, we also connect the two well studied problems of sphere packing and tetrahedron packing on a single axis. Our numerical results uncover a rich optimal-packing behavior, compared to that of other continuous families of particles previously studied. We present four structures as candidates for the optimal packing at different values of the parameter, providing an atlas of crystal structures which might be observed in systems of nano-particles with tetrahedral symmetry