Killer immunoglobulin-like Receptors (KIR) haplogroups A and B track with Natural Killer Cells and Cytokine Profile in Aged Subjects: Observations from Octo/Nonagenarians in the Belfast Elderly Longitudinal Free-living Aging STudy (BELFAST)

BACKGROUND: Natural Killer Cells (NK) play an important role in detection and elimination of virus-infected, damaged or cancer cells. NK cell function is guided by expression of Killer Immunoglobulin-like Receptors (KIRs) and contributed to by the cytokine milieu. KIR molecules are grouped on NK cells into stimulatory and inhibitory KIR haplotypes A and B, through which NKs sense and tolerate HLA self-antigens or up-regulate the NK-cytotoxic response to cells with altered HLA self-antigens, damaged by viruses or tumours. We have previously described increased numbers of NK and NK-related subsets in association with sIL-2R cytokine serum levels in BELFAST octo/nonagenarians. We hypothesised that changes in KIR A and B haplotype gene frequencies could explain the increased cytokine profiles and NK compartments previously described in Belfast Elderly Longitudinal Free-living Aging STudy (BELFAST) octo/nonagenarians, who show evidence of ageing well. RESULTS: In the BELFAST study, 24% of octo/nonagenarians carried the KIR A haplotype and 76% KIR B haplotype with no differences for KIR A haplogroup frequency between male or female subjects (23% v 24%; p=0.88) or for KIR B haplogroup (77% v 76%; p=0.99). Octo/nonagenarian KIR A haplotype carriers showed increased NK numbers and percentage compared to Group B KIR subjects (p=0.003; p=0.016 respectively). There were no KIR A/ B haplogroup-associated changes for related CD57+CD8 ((high or low)) subsets. Using logistic regression, KIR B carriers were predicted to have higher IL-12 cytokine levels compared to KIR A carriers by about 3% (OR 1.03, confidence limits CI 0.99–1.09; p=0.027) and 14% higher levels for TGF-β (active), a cytokine with an anti-inflammatory role, (OR 1.14, confidence limits CI 0.99–1.09; p=0.002). CONCLUSION: In this observational study, BELFAST octo/nonagenarians carrying KIR A haplotype showed higher NK cell numbers and percentage compared to KIR B carriers. Conversely, KIR B haplotype carriers, with genes encoding for activating KIRs, showed a tendency for higher serum pro-inflammatory cytokines compared to KIR A carriers. While the findings in this study should be considered exploratory they may serve to stimulate debate about the immune signatures of those who appear to age slowly and who represent a model for good quality survivor-hood

Working on the edge: healthy environments in hospitals and offices

Two recently started research projects in the research line of “Creating Healthy Environments” will be described. They focus on improving comfort, health and safety using smart and innovative designs and solutions for the built environment in hospital and office environments respectively. Solutions for healthy environments will not result from optimizing singular aspects but from a holistic approach considering multiple contributing parameters. The overall objective of the hospital project is to gain more insight into the relation between lighting conditions on one hand, and user well-being as well as their performance in a hospital environment. The focus is mainly on staff members but will include investigations with patients as well. The expected results from this research project are addressing this lack of consideration but identifying the impact factors on the health care staff satisfaction. Recommendations derived from the research results will inform the design process and help guiding the design towards a healing environment that has a positive motivating effect on staff leading to for instances shorter hospital stays of patients and reduction of medical errors. The research is being carried out by 2 PhD students (one focusing on lighting and the other on acoustics). The focus of the office project is on the interaction of staff with the lighting system and on their usage of the office environment. The connected lighting system of Philips which is being used in the new office building “The Edge” allows retrieving anonymous data on the usage and interaction with the lighting and climate system. Its impact on the health and well-being of workers as well as the overall strategy for efficiently operating these end uses form the research carried out by a total of 4 PhD students. The expected results and insights will contribute to recommendations for enhancing the effectiveness of a lit office environment without negatively impacting the well-being and motivation of staff

How Not to Become a Buffoon in Front of a Shop Window: A Solution Allowing Natural Head Movement for Interaction with a Public Display

The user interaction solution described in this paper was developed in the context of an Intelligent Shop Window (ISW) with an aim to offer a user the interaction solution where system response would be triggered by naturally gazing at products. We have analyzed a possibility to realize such a user interaction solution using gaze tracking and concluded that remote calibration free eye tracking is still a subject of academic research, but that head tracking could be used instead. We argue that conventional use of head tracking requires conscious intentional head movements and thus does not fit into the context of applications such as the ISW. We further describe our experiment aimed to explore how head movements relate to eye movements when looking at objects in a shop window context. We show large variability in head movement and that per individual the gaze-head data could well be approximated with a straight line. Based on these results we propose a new solution that enables natural gaze interaction by means of head tracking

How not to become a buffoon in front of a shop window : a solution allowing natural head movement for interaction with a public display

The user interaction solution described in this paper was developed in the context of an Intelligent Shop Window (ISW) with an aim to offer a user the interaction solution where system response would be triggered by naturally gazing at products. We have analyzed a possibility to realize such a user interaction solution using gaze tracking and concluded that remote calibration free eye tracking is still a subject of academic research, but that head tracking could be used instead. We argue that conventional use of head tracking requires conscious intentional head movements and thus does not fit into the context of applications such as the ISW. We further describe our experiment aimed to explore how head movements relate to eye movements when looking at objects in a shop window context. We show large variability in head movement and that per individual the gaze-head data could well be approximated with a straight line. Based on these results we propose a new solution that enables natural gaze interaction by means of head tracking

Feasibility of ceiling-based luminance distribution measurements

There is a high relevancy in the luminance distribution related to the perceived visual comfort. Moreover, the required technology is maturing such that it is feasible to integrate such devices in lighting control systems, which is expected to improve the overall lighting quality in office environments, or to conduct long-term field studies. Preferably, the luminance distribution measurement corresponds to the visual field of the user. However, for long-term measurements this is not feasible as this causes interference. Therefore, this study aimed to find a suitable ceiling-based position for luminance distribution measurements. In a first phase, the most suitable ceiling-based measurement position was identified for four luminance based metrics: Desktop Luminance, Monitor Luminance, B40 Luminance, and Retinal Illuminance. The results showed that a ceiling-based position above the aisle with a 20° angle relative to the ceiling was the most suitable position because its field of view has large similarities with the field and angle of view of the user. In a second phase, the performance of this most suitable position found in phase 1 was assessed under real office conditions, and compared with the visual field of the user. The Desktop and Monitor Luminance achieved an acceptable accuracy with very basic commissioning. The Retinal illuminance was measured with a reasonable accuracy when an elaborate calibration procedure was applied. For the B40 Luminance, in all scenarios, inaccuracies above 20% were found. This study shows that ceiling-based measurements are feasible, except for the B40 luminance, however, one should account for the introduced uncertainty

Recommendations for long-term luminance distribution measurements:the spatial resolution

Currently, luminance distribution measurement devices are increasingly used because there is high relevancy in the luminance distribution to the perceived visual comfort, also technology is maturing. It is now feasible to conduct long-term measurements and integrate these devices into lighting control systems. This, however, can result in new issues such as privacy controversies and high computational costs, induced by high spatial resolutions. Therefore, this study aims to propose a spatial resolution that is able to measure the luminance accurately while minimizing privacy sensitivity and computational costs. This is done based on luminance distribution measurements in office environments. The accuracy of lower resolution luminance distributions is tested for the mean and maximum luminance and the illuminance. Additionally, the ability to recognize faces is measured as an indicator for privacy-sensitive content. Finally, the processing time is measured as an indicator for the computational costs. The results show that for mean luminance or illuminance measurements the spatial resolution can be reduced significantly to 440 x 330 and 720 x 540 pixels, respectively. This spatial resolution does not compromise the accuracy while minimizing the ability of automated facial recognition and reducing the computational costs significantly. However, for maximum luminance measurements, a high resolution of 3000 x 2250 pixels is deemed appropriate, although this does allow automated facial recognition and results in high computation costs. A toolbox has been developed to assist others in choosing a relevant spatial resolution for their luminance camera during long term luminance measurements in typical office environments

Spectral tuning of luminance cameras: a theoretical model and validation measurements

Presently, luminance distribution measurement devices, using High Dynamic Range technology, are increasingly used as they provide a lot of relevant data related to the lit environment at once. However, the accuracy of these devices can be a concern. It is expected that the accuracy would be improved by incorporating the effect of the camera spectral responsivity and the spectral power distribution of the illuminant under which the measurements are conducted. This study introduces two optimization criteria incorporating these aspects to improve the spectral match and the performance of luminance distribution measurement devices. Both criteria are tested in a theoretical model and in practical measurements using two cameras and three illuminants: LED, halogen and fluorescent. Both methodologies support the hypothesis that the conventional method to determine the luminance introduces spectral mismatches that can be limited by optimizing relative to the spectral responsivity of the camera. Additionally, substantial evidence was found, by both the theoretical model and the validation measurements, that the spectral power distribution of the illuminant also has an effect on the performance

Why Directionality Is an Important Light Factor for Human Health to Consider in Lighting Design?

Both image-forming and non-image-forming effects of radiation require proper attention in lighting design that aims at meeting human vision and health requirements. Intrinsically Photosensitive Retinal Ganglion Cells (ipRGCs) appear to play an essential role in stimulation of the non-image forming effects and thus human health and well-being. There are indications that radiation incident contributes to the magnitude of these effects. This review summarizes current studies on humans and animals related to radiation directionality as well as the spatial distribution of ipRGCs on the retina. New insights can facilitate and optimize the incorporation of radiation directionality in building lighting design