Lactic Acid Bacteria against Listeria monocytogenes

Background: Listeria monocytogenes is a pathogenic bacterium that can contaminate food and cause public health problems due its ability to form biofilms and resistance to sanitizers, it is responsible for sanitary and economic losses in food producing establishments. The difficulties in controlling biofilms and increasing resistance to traditional antibacterial agents is motivating studies of alternative potential biological agents for the control of pathogenic biofilms, among which lactic acid bacteria (LABs) are included. The objective of this work was to evaluate the activity of LABs against Listeria monocytogenes biofilm formation on polystyrene plates, a surface commonly used in the food industry.Materials, Methods & Results: Lyophilized commercial strains of Bifidobacterium animalis, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus salivaris and Lactobacillus acidophilus were used. The strain of Listeria monocytogenes (L4) was isolated from polystyrene mats from a poultry slaughterhouse cutting room and demonstrated the ability to attach to microplates and resistance to sanitizers (sodium hypochlorite and hydrogen peroxide) at all times, temperatures and tested surfaces. The antimicrobial activity of LABs was evaluated by the agar diffusion method. The LABs that presented action on Listeria monocytogenes were selected for the inhibition and/or removal of biofilms in microplates, and all experiments were carried out in triplicate. Only Bifidobacterium animalis and Lactobacillus plantarum demonstrated action against Listeria. monocytogenes in the agar diffusion assays and were selected for inhibition and competition assays. Furthermore, competition of LABs against Listeria monocytogenes adhesion was evaluated. There was no significant difference between LABs and Listeria monocytogenes, alone or in combination, at temperatures of 30ºC and 37ºC in the Listeria monocytogenes inhibition assays on polystyrene surface. The lactic acid bacteria evaluated did not demonstrate inhibition of Listeria monocytogenes adhesin testes with optical density visualization, however, it was possible to identify a reduction in Listeria monocytogenes counts with the application of Bifidobacterium animals and Lactobacillus plantarum in the testes of competition against biofilm formation. In competition tests Bifidobacterium animalis and Lactobacillus plantarum have an injunction in Listeria monocytogenes, indicating that these lactic acid bacteria can retard Listeria biofilm formation on polystyrene surfaces and thus help control the pathogen in the food industry.Discussion: A potential mechanism to control biofilm adhesion and formation of pathogens for nutrients and fixation on surfaces, multiplication factors and surfaces are a challenge in controlling biofilms of pathogenic microorganisms, alternative measures to traditional methods for inactivating pathogens and biofilm formers bacteria are necessary. In this sense, lactic acid bacteria generate high levels of bacteriocin and are effective in inhibiting the biofilm of pathogenic bacteria, however, our study did not reveal this. We verified that Bifidobacterium animalis and Lactobacillus plantarum have an inhibitory action on Listeria monocytogenes, indicating that these lactic acid bacteria can be used to delay the formation of biofilms by Listeria on polystyrene surfaces, helping to control this pathogen in food industry.Keywords: control of biofilm, pathogenic bacteria, food industry, polystyrene surface, FTDs

MANEJO FLORESTAL EMPRESARIAL NO ACRE: CUSTOS DA ATIVIDADE PRÉ-EXPLORATÓRIA NA FLORESTA ESTADUAL DO ANTIMARY.

Esta pesquisa aborda a atividade madeireira sob o regime de concessão florestal desenvolvido no Estado do Acre. Os objetivos do presente estudo são: identificar o custo da produção do manejo florestal sustentável (MFS) para produção madeireira, tendo como base a Floresta Estadual do Antimary (FEA); e, quantificar o valor, a ser pago pela terra, numa área destinada como floresta pública de produção, no ano de 2005. Para tal, foi considerada, como premissa básica, um ciclo de corte de 15 anos. As técnicas usadas identificaram o custo de produção da madeira com origem em uma florestal manejada (Cmf), o valor presente líquido (VPL) do MFS e o valor esperado da terra (VET), os quais foram calculados considerando as taxas de desconto de 4%, 6%, 8%, 10% e 12% ao ano. Os principais resultados obtidos foram: a uma taxa de desconto de 4% ao ano, uma empresa gastaria, com o manejo florestal, US$68,50/ha e geraria um VPL de US$ 92,18/ha. Por outro lado, a 12% ao ano, estes valores decresceriam para US$62,53/ha e US$ 81,34/ha, respectivamente. O preço máximo que poderia ser pago por um hectare de terra na FEA, em 2005, para que o MFS fosse remunerado numa taxa de 4% a.a., foi de US$218,17/ha, visto que, a partir de 6$ 199,03/ha).---------------------------------------------This research addresses the timber activity under a forest concession system in the State of Acre. Its objectives are: to identify the production cost related to sustainable forest management (SFM) for timber yield, having as scenario the Antimary State Forest (ASF); and, quantify the value to be paid for the land, in an area designed as public production forest, in the year 2005. So, it was recognized as basic foundation, a 15-year cutting cycle. The techniques used identified the production cost of log coming from a managed forest area (Cmf), the net present value (NPV) concerning SFM and the land expectation value (LEV), which were calculated adopting annual interest rates of 4%, 6%, 8%, 10% and 12%. The main results obtained were: at annual interest rate of 4%, a firm would spend, with forest management US$68,50/ha and would generate US$ 92,18/ha as NPV. On the another hand, at 12% a.a., these value would decrease to US$62,53/ha and US$ 81,34/ha, respectively. The maximum price which could be paid for one hectare of land in the ASF, in 2005, in order to remunerate SFM at 4% a.a., was US$218,17/ha, because adopting interest rates greater than 6$ 199,03/ha).Manejo florestal sustentável, Custo da produção madeireira, Economia florestal, Valor esperado da terra, Sustainable forest management, timber production cost, Forest economics, Land expectation value, Resource /Energy Economics and Policy,

Praça Barão do Rio Branco satisfação da população de Cáceres-MT após a reforma e readequação / Praça Barão do Rio Branco satisfaction of the population of Cáceres-MT after the reform and readjustment

A praça é um espaço público, em geral identificado como um ponto de referência no contexto urbano, também é um local conhecido por proporcionar momentos de convívio entre as pessoas, historicamente palco de movimentos, culturais, religiosos, políticos e comerciais. A Praça Barão do Rio Branco está localizada na cidade de Cáceres Mato Grosso sendo considerada um espaço de lazer pela população. O objetivo do trabalho foi saber a opinião dos usuários da Praça Barão do Rio Branco com relação a sua reforma e readequação ocorrida no ano de 2017. Sendo assim, foi elaborado um questionário estruturado analisando o perfil dos frequentadores, os hábitos de utilização e a percepção conservacionista dos frequentadores. Quando os usuários foram questionados sobre a satisfação em relação a reforma da praça 90% disseram estar satisfeitos, 50% das pessoas frequentam a praça diariamente e 72,7% usam a praça como ambiente de lazer. Após a reforma é notado uma intensificação no uso da praça, atendendo as várias demandas dos frequentadores. Sendo assim, podemos concluir, que a reforma da Praça Bração do Rio Branco, obteve resultados positivos e que de forma geral, a população aprova as intervenções realizadas

Projeto educação em saúde na escola família agrícola de Jaboticaba - Quixabeira-BA: experiência de uma oficina de saúde mental / Health education project at the family agricultural school of Jaboticaba - Quixabeira-BA: experience of a mental health workshop

Este trabalho relata experiência de uma oficina em saúde mental realizada como parte de um projeto de educação em saúde na Escola Família Agrícola de Jaboticaba na cidade de Quixabeira – Bahia. Teve como objetivo geral promover educação em saúde mental para estudantes da referida escola e como objetivos específicos: promover saúde mental através da educação em saúde, conhecer os tipos de relacionamento, discutir o conceito de subjetividade, promover a reflexão sobre os tipos de conflitos, refletir sobre o conceito de saúde mental, construir um Projeto Terapêutico Singular, conhecer estratégias de cuidado em saúde mental, instruir sobre pontos de atenção à saúde mental presentes no município de Quixabeira – Bahia e construir um projeto de vida nos contextos pessoal e coletivo. A oficina foi mediada por docente especialista em saúde mental e cinco acadêmicos da Faculdade AGES de Medicina, quatro do primeiro período e um do segundo período. Houve repercussões positivas geradas pela realização da oficina sobre saúde mental, uma vez que foi possível observar a participação e o engajamento dos alunos no debate e nas atividades propostas. As ações do projeto, mesmo que pontuais, contribuíram para a troca de conhecimento e de experiências entre os participantes e os estudantes de Medicina, pela proximidade entre as faixas etárias e pelo intermédio do método utilizado (dinâmicas), sendo esse diálogo preponderante para instigar reflexões sobre saúde mental e autocuidado, importância de buscar um projeto de vida no contexto pessoal e coletivo, de modo que esse planejamento também contribuísse para a análise dos conflitos nos ambientes escolar, familiar e social

Prevalência do infarto agudo do miocárdio em mulheres e fatores de risco associados

This article aims to analyze the risk factors associated with acute myocardial infarction in women. The study is an Integrative Literature Review based on a retrospective cross-sectional study of a descriptive nature and a qualitative data approach. The research was carried out in six stages: elaboration of the research question; search and sampling – establishment of inclusion and exclusion criteria; data extraction; evaluation and critical analysis of the data obtained; analysis and synthesis of studies with interpretation of results; presentation of the review. For the guiding research question, the PICO strategy was considered. The selection of articles was made in the databases PubMed, Medical Literature Analysis and Retrievel System Online (MedLine), Science Direct and Latin American and Caribbean Literature in Health Sciences (Lilacs). In the search strategy, combinations of descriptors were considered according to the indications of each database, namely the Health Sciences Descriptors (DeCS) of the Virtual Health Library (VHL) and Medical Subject Headings (MeSH). The most prevalent risk factors associated with acute myocardial infarction in women are: advanced age, hormone replacement therapy, use of oral contraceptives, genetic predisposition and emotional overload. Therefore, it is important to encourage health education related to search strategies aimed at early diagnosis of this clinical condition in females. Targeted anamnesis is essential for the aforementioned condition in order to obtain a good prognosis.Este artículo tiene como objetivo analizar los factores de riesgo asociados al infarto agudo de miocardio en la mujer. El estudio es una Revisión Integrativa de la Literatura basada en un estudio transversal retrospectivo de carácter descriptivo y enfoque de datos cualitativos. La investigación se desarrolló en seis etapas: elaboración de la pregunta de investigación; búsqueda y muestreo – establecimiento de criterios de inclusión y exclusión; extracción de datos; evaluación y análisis crítico de los datos obtenidos; análisis y síntesis de estudios con interpretación de resultados; presentación de la reseña. Para la pregunta rectora de la investigación se consideró la estrategia PICO. La selección de artículos se realizó en las bases de datos PubMed, Medical Literature Analysis and Retrievel System Online (MedLine), Science Direct y Latin American and Caribbean Literature in Health Sciences (Lilacs). En la estrategia de búsqueda se consideraron combinaciones de descriptores según las indicaciones de cada base de datos, concretamente los Descriptores en Ciencias de la Salud (DeCS) de la Biblioteca Virtual en Salud (BVS) y los Medical Subject Headings (MeSH). Los factores de riesgo más prevalentes asociados al infarto agudo de miocardio en la mujer son: edad avanzada, terapia de reemplazo hormonal, uso de anticonceptivos orales, predisposición genética y sobrecarga emocional. Por lo tanto, es importante incentivar la educación en salud relacionada con estrategias de búsqueda encaminadas al diagnóstico precoz de esta condición clínica en el sexo femenino. La anamnesis dirigida es fundamental para obtener un buen pronóstico de la enfermedad antes mencionada.Este artigo tem por objetivo analisar os fatores de risco associados ao infarto agudo do miocárdio em mulheres. O estudo se trata de uma Revisão Integrativa de Literatura baseada em um estudo transversal retrospectivo de caráter descritivo e abordagem qualitativa dos dados. A pesquisa foi realizada em seis etapas: elaboração da questão de pesquisa; busca e amostragem – estabelecimento de critérios de inclusão e exclusão; extração de dados; avaliação e análise crítica dos dados obtidos; análise e síntese dos estudos com interpretação dos resultados; apresentação da revisão. Para a questão norteadora da pesquisa, foi considerada a estratégia PICO. A seleção dos artigos foi feita nas bases de dados PubMed, Medical Literature Analysis and Retrievel System Online (MedLine), Science Direct e Literatura Latino-Americana e do Caribe em Ciências da Saúde (Lilacs). Na estratégia de busca foram consideradas combinações de descritores conforme as indicações de cada base de dados, sendo eles os Descritores em Ciências da Saúde (DeCS) da  Biblioteca Virtual em Saúde (BVS) e Medical Subject Headings (MeSH). Os fatores de risco mais prevalentes associados ao infarto agudo do miocárdio em mulheres são: idade avançada, terapia de reposição hormonal, uso de anticoncepcionais orais, predisposição genética e sobrecarga emocional. Dessa forma, é importante o estímulo da educação em saúde relacionada a estratégias de busca que visem o diagnóstico precoce dessa condição clínica no sexo feminino. A anamnese direcionada é fundamental para o quadro supracitado a fim de se obter bom prognóstico

Psychological well-being in Europe after the outbreak of war in Ukraine

The Russian invasion of Ukraine on February 24, 2022, has had devastating effects on the Ukrainian population and the global economy, environment, and political order. However, little is known about the psychological states surrounding the outbreak of war, particularly the mental well-being of individuals outside Ukraine. Here, we present a longitudinal experience-sampling study of a convenience sample from 17 European countries (total participants = 1,341, total assessments = 44,894, countries with >100 participants = 5) that allows us to track well-being levels across countries during the weeks surrounding the outbreak of war. Our data show a significant decline in well-being on the day of the Russian invasion. Recovery over the following weeks was associated with an individual’s personality but was not statistically significantly associated with their age, gender, subjective social status, and political orientation. In general, well-being was lower on days when the war was more salient on social media. Our results demonstrate the need to consider the psychological implications of the Russo-Ukrainian war next to its humanitarian, economic, and ecological consequences

A global experience-sampling method study of well-being during times of crisis : The CoCo project

We present a global experience-sampling method (ESM) study aimed at describing, predicting, and understanding individual differences in well-being during times of crisis such as the COVID-19 pandemic. This international ESM study is a collaborative effort of over 60 interdisciplinary researchers from around the world in the “Coping with Corona” (CoCo) project. The study comprises trait-, state-, and daily-level data of 7490 participants from over 20 countries (total ESM measurements = 207,263; total daily measurements = 73,295) collected between October 2021 and August 2022. We provide a brief overview of the theoretical background and aims of the study, present the applied methods (including a description of the study design, data collection procedures, data cleaning, and final sample), and discuss exemplary research questions to which these data can be applied. We end by inviting collaborations on the CoCo dataset

Psychological well-being in Europe after the outbreak of war in Ukraine

The Russian invasion of Ukraine on February 24, 2022, has had devastating effects on the Ukrainian population and the global economy, environment, and political order. However, little is known about the psychological states surrounding the outbreak of war, particularly the mental well-being of individuals outside Ukraine. Here, we present a longitudinal experience-sampling study of a convenience sample from 17 European countries (total participants = 1,341, total assessments = 44,894, countries with >100 participants = 5) that allows us to track well-being levels across countries during the weeks surrounding the outbreak of war. Our data show a significant decline in well-being on the day of the Russian invasion. Recovery over the following weeks was associated with an individual’s personality but was not statistically significantly associated with their age, gender, subjective social status, and political orientation. In general, well-being was lower on days when the war was more salient on social media. Our results demonstrate the need to consider the psychological implications of the Russo-Ukrainian war next to its humanitarian, economic, and ecological consequences

A global experience-sampling method study of well-being during times of crisis : the CoCo project

[Corrections added on 5 July 2023 after first online publication: The authorship footnote has been modified on page 1 and the duplicate phrase “experience sampling” has been removed on page 2.]We present a global experience-sampling method (ESM) study aimed at describing, predicting, and understanding individual differences in well-being during times of crisis such as the COVID-19 pandemic. This international ESM study is a collaborative effort of over 60 interdisciplinary researchers from around the world in the “Coping with Corona” (CoCo) project. The study comprises trait-, state-, and daily-level data of 7490 participants from over 20 countries (total ESM measurements = 207,263; total daily measurements = 73,295) collected between October 2021 and August 2022. We provide a brief overview of the theoretical background and aims of the study, present the applied methods (including a description of the study design, data collection procedures, data cleaning, and final sample), and discuss exemplary research questions to which these data can be applied. We end by inviting collaborations on the CoCo dataset.Deutsche Forschungsgemeinschaft.https://wileyonlinelibrary.com/journal/spc3am2024PsychologySDG-03:Good heatlh and well-bein

Penilaian Kinerja Keuangan Koperasi di Kabupaten Pelalawan

This paper describe development and financial performance of cooperative in District Pelalawan among 2007 - 2008. Studies on primary and secondary cooperative in 12 sub-districts. Method in this stady use performance measuring of productivity, efficiency, growth, liquidity, and solvability of cooperative. Productivity of cooperative in Pelalawan was highly but efficiency still low. Profit and income were highly, even liquidity of cooperative very high, and solvability was good