Career roles:their Measurements, Determinants and Consequences

Over time, depending on their traits and preferences, people will select and enact certain roles in their jobs which may grow into enduring career roles (which are coherent and enduring sets of characteristics describing the way a person is doing his or her work; Hoekstra, 2011, p164). In this dissertation we presented a new measure for career role identification, the Career Role Identification Questionnaire (CRIQ). Making use of the Comparison Awareness Inducing Technique we were able to reduce the effects of self-presentation tactics in survey responses. Furthermore, the results in this dissertation indicate that peoples’ personality traits predict which roles they prefer to have in the work context, which, in turn, predict the career roles they have or expect to have in the future. In addition, career roles may focus on exploration (targeting innovation and change) or on exploitation behavior (targeting production and results). Our results indicate that especially exploration career roles contribute towards employee employability. With this research we aim to contribute to further conceptualize and provide meaning to all career positions and pathways that are present in the current career landscape

Methods of optimization for KCE-based aptamer selection

The success of KCE-based aptamer selection relies on three distinct steps of pre-selection optimization: (i) preventing protein adsorption to the inner walls of the capillary, (ii) maximizing the protein-DNA separation, and (iii) determining an appropriate aptamer-collection window. To perform the first step of optimization, we have developed a simple pressure-based approach which can qualitatively characterize protein adsorption on capillary walls in CE1 o Conceptually, a short plug containing the protein solution is injected into the capillary and carried towards the detection point by applying a low pressure. A dual on-column detector is mimicked by repeating the experiment using the same capillary but a shortened distance to the detection window. The temporal propagation pattern of the protein, at each detection distance, is recorded and the degree of adsorption then analyzed by comparing peak areas and symmetry. This process can be repeated using different buffer additives2, dynamic or permanent coatings until optimal conditions are established. The remaining two steps of pre-selection optimization can be solved by fluorescently labelling the target protein so that its compatibility with KCE separation is maintained3 o By labeling the protein with Chromeo P503, we demonstrate that target detection in CE is markedly improved without significantly affecting the proteins electrophoretic mobility or ability to bind DNA. Thus, Chromeo-labelling can facilitate the accurate identification of both the protein and protein-aptamer complex, which is necessary for maximizing protein-DNA separation and selecting the aptamer collection window. Target-specific considerations must also be optimized prior to commencement of KCE-SELEX. Exonuclease targets, such as Exonuclease I (E.coli), recognize the DNA library as a substrate to be degraded. As a result, the enzyme must be inactivated while still maintaining its native three-dimensional structure during aptamer collection. In addition, non-specific, or unwanted binding at the active site must be avoided, as collection of non-aptamers would limit the progress of KCE-SELEX. For Exol, a divalent metal chelating agent was found to effectively suppress library degradation through experimental optimization. In addition, a small oligonucleotide-based competitive inhibitor was found to bind to the active site with high affinity. This effectively eliminates any unwanted binding of the DNA library at the substrate binding site. The Exol-inhibitor complex can then serve as a target for aptamer development towards an allosteric site of the protein

CRIQ: An innovative measure using comparison awareness to avoid self-presentation tactics

The article presents a new measure for career role identification, the Career Role Identification Questionnaire (CRIQ). In constructing the CRIQ, we used the Comparison Awareness Inducing Technique (CAIT), a new and innovative method to reduce the effects of self-presentation tactics. The results show that the CRIQ measures identification with the six career roles conceptualized by Hoekstra (2011). The inventory has reliable scales and a clear factorial structure. Furthermore, the CAIT receives some support as a new way to deal with the problem of social desirability in self-report measures. The CAIT technique is thought to induce comparison awareness and thus suppress various response tendencie

Thrombin generation during cardiopulmonary bypass: the possible role of retransfusion of blood aspirated from the surgical field

BACKGROUND: In spite of using heparin-coated extracorporeal circuits, cardiopulmonary bypass (CPB) is still associated with an extensive thrombin generation, which is only partially suppressed by the use of high dosages of heparin. Recent studies have focused on the origins of this thrombotic stimulus and the possible role of retransfused suctioned blood from the thoracic cavities on the activation of the extrinsic coagulation pathway. The present study was designed to find during CPB an association between retransfusion of suctioned blood from the pericardium and pleural space, containing activated factor VIIa and systemic thrombin generation. METHODS: Blood samples taken from 12 consenting patients who had elective cardiac surgery were assayed for plasma factor VIIa, prothrombin fragment 1+2 (F(1+2)), and thrombin-antithrombin (TAT) concentrations. Blood aspirated from the pericardium and pleural space was collected separately, assayed for F(1+2), TAT, and factor VIIa and retransfused to the patient after the aorta occlusion. RESULTS: After systemic heparinization and during CPB thrombin generation was minimal, as indicated by the lower than base line plasma levels of F(1+2), and TAT after correction for hemodilution. In contrast, blood aspirated from the thoracic cavities had significantly higher levels of factor VIIa, F(1+2), and TAT compared to the simultaneous samples from the blood circulation (P < 0.05). Furthermore, after retransfusion of the suctioned blood (range, 200–1600 mL) circulating levels of F(1+2), and TAT rose significantly from 1.6 to 2.9 nmol/L (P = 0.002) and from 5.1 to 37.5 μg/L (P = 0.01), respectively. The increase in both F(1+2), and TAT levels correlated significantly with the amount of retransfused suctioned blood (r = 0.68, P = 0.021 and r = 0.90, P = 0.001, respectively). However, the circulating factor VIIa levels did not correlate with TAT and F(1+2 )levels. CONCLUSIONS: These data suggest that blood aspirated from the thoracic cavities during CPB is highly thrombogenic. Retransfusion of this blood may, therefore, promote further systemic thrombin generation during CPB

Midwives’ occupational wellbeing and its determinants:A cross-sectional study among newly qualified and experienced Dutch midwives

Objective: Internationally, about 40 percent of midwives report symptoms of burnout, with young and inexperienced midwives being most vulnerable. There is a lack of recent research on burnout among Dutch midwives. The aim of this study was to examine the occupational wellbeing and its determinants of newly qualified and inexperienced midwives in the Netherlands. The majority of practicing Dutch midwives are aged under 40, which could lead to premature turnover.Design: A cross-sectional study was conducted using an online questionnaire that consisted of validated scales measuring job demands, job and personal resources, burnout symptoms and work engagement. The Job Demands-Resources model was used as a theoretical model.Setting and participants: We recruited Dutch midwives who were actually working in midwifery practice. A total of N=896 midwives participated in this study, representing 28 percent of practicing Dutch midwives.Measurements and Findings: Data were analysed using regression analysis. Seven percent of Dutch midwives reported burnout symptoms and 19 percent scored high on exhaustion. Determinants of burnout were all measured job demands, except for experience level. Almost 40 percent of midwives showed high work engagement; newly qualified midwives had the highest odds of high work engagement. Master's or PhD-level qualifications and employment status were associated with high work engagement. All measured resources were associated with high work engagement.Key conclusions: A relatively small percentage of Dutch midwives reported burnout symptoms, the work engagement of Dutch midwives was very high. However, a relatively large number reported symptoms of exhaustion, which is concerning because of the risk of increasing cynicism levels leading to burnout. In contrast to previous international research findings, being young and having less working experience was not related to burnout symptoms of Dutch newly qualified midwives.Implications for practice: The recognition of job and personal resources for midwives’ occupational wellbeing must be considered for a sustainable midwifery workforce. Midwifery Academies need to develop personal resources of their students that will help them in future practice.</p

Synthesis and characterization of bifunctional dendrimers: preliminary use for the coating of gold surfaces and the proliferation of human osteoblasts (HOB)

Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Two different novel families of bifunctional water-soluble dendrimers are synthesized, using the specific functionalization of one function of the cyclotriphosphazene core. Dendrimers are grown from the 5 remaining functions, up to generation 2. Water-solubility is attained in the last step of the synthesis by grafting either ammonium terminal groups or carboxylate terminal groups, on generations 1 and 2 of these bifunctional dendrimers. 12 new compounds are synthesized and fully characterized, in particular by multi-nuclear NMR. The function linked to the core is thioctic acid, suitable for grafting onto gold, thus both types of water-soluble dendrimers can be used to coat gold surfaces. These macromolecular assemblies are characterized by surface plasmon resonance (SPR). In a preliminary attempt, the gold surfaces modified by either positively or negatively charged dendrimers are used for studying their interaction with cells. Exposed to human osteoblast cells (OBC), the influence of the surface coatings on the cell responses is investigated. Polycationic dendrimers provoke cell apoptosis, whereas negatively charged dendrimers support cell adhesion and proliferation

RNA Bind-n-Seq: Quantitative Assessment of the Sequence and Structural Binding Specificity of RNA Binding Proteins

Specific protein-RNA interactions guide posttranscriptional gene regulation. Here, we describe RNA Bind-n-Seq (RBNS), a method that comprehensively characterizes sequence and structural specificity of RNA binding proteins (RBPs), and its application to the developmental alternative splicing factors RBFOX2, CELF1/CUGBP1, and MBNL1. For each factor, we recovered both canonical motifs and additional near-optimal binding motifs. RNA secondary structure inhibits binding of RBFOX2 and CELF1, while MBNL1 favors unpaired Us but tolerates C/G pairing in motifs containing UGC and/or GCU. Dissociation constants calculated from RBNS data using a novel algorithm correlated highly with values measured by surface plasmon resonance. Motifs identified by RBNS were conserved, were bound and active in vivo, and distinguished the subset of motifs enriched by CLIP-Seq that had regulatory activity. Together, our data demonstrate that RBNS complements crosslinking-based methods and show that in vivo binding and activity of these splicing factors is driven largely by intrinsic RNA affinity.National Science Foundation (U.S.) (0821391

Dice-and-fill single element octagon transducers for next generation 3D USCT

At the Karlsruhe Institue of Technology (KIT), a 3D-Ultrasound Computer Tomography (3D-USCT) medical imaging system for early breast cancer detection is currently developed. With the next generation of 3D-USCT 2.5, the current region of interest (ROI) of 10 x 10 x 10 cm³ shall be increased to 20 x 20 x 20 cm³ to allow reliable imaging results also for bigger female breasts. Therefore, the opening angle (OA) of the future transducers should be increased to approx. 60° at 3 dB while other characteristics such as bandwidth (BW) and resonance frequency should be preserved or even improved. Based on the current dice-andfill approach in transducer production, optimization is performed on piezoelectric sensor geometry and size, type and structure of matching and backing layer and interconnection technology of the several parts of the transducer

Dietary patterns in an elderly population and their relation with bone mineral density: the Rotterdam Study

Purpose: Our aim was to identify dietary patterns that are associated with bone mineral density (BMD) against a background of relatively high dairy intake in elderly Dutch subjects. Methods: Participants were 55 years of age and older (n = 5144) who were enrolled in The Rotterdam Study, a population-based prospective cohort study. Baseline intake of 28 pre-defined food groups was determined using a validated food frequency questionnaire. Dietary patterns were identified using principal component analysis. BMD was measured using dual-energy X-ray absorptiometry at baseline and at three subsequent visits (between 1993 and 2004). Linear mixed modelling was used to longitudinally analyse associations of adherence to each pattern with repeatedly measured BMD (both in Z scores). Results: After adjustment for confounders, two dietary patterns were associated with high BMD: a “Traditional” pattern, characterized by high intake of potatoes, meat and fat (β = 0.06; 95 % CI 0.03, 0.09) and a “Health conscious” pattern, characterized by high intake of fruits, vegetables, poultry and fish (β = 0.06; 95 % CI 0.04, 0.08). The “Processed” pattern, characterized by high intake of processed meat and alcohol, was associated with low BMD (β = −0.03; 95 % CI −0.06, −0.01). Associations of adherence to the “Health conscious” and “Processed” pattern with BMD were independent of body weight and height, whereas the association between adherence to the “Traditional” pattern with BMD was not. Conclusions: Against a background of high dairy intake and independent of anthropometrics, a “Health conscious” dietary pattern may have benefits for BMD, whereas a “Processed” dietary pattern may pose a risk for low BMD

Adherence to a plant-based, high-fibre dietary pattern is related to regression of non-alcoholic fatty liver disease in an elderly population

Dietary lifestyle intervention is key in treating non-alcoholic fatty liver disease (NAFLD). We aimed to examine the longitudinal relation between well-established dietary patterns as well as population-specific dietary patterns and NAFLD. Participants from two subsequent visits of the Rotterdam Study were included. All underwent serial abdominal ultrasonography (median follow-up: 4.4 years) and filled in a food frequency questionnaire. Secondary causes of steatosis were excluded. Dietary data from 389 items were collapsed into 28 food groups and a posteriori dietary patterns were identified using factor analysis. Additionally, we scored three a priori dietary patterns (Mediterranean Diet Score, Dutch Dietary Guidelines and WHO-score). Logistic mixed regression models were used to examine the relation between dietary patterns and NAFLD. Analyses were adjusted for demographic, lifestyle and metabolic factors. We included 963 participants of whom 343 had NAFLD. Follow-up data was available in 737 participants. Incident NAFLD was 5% and regressed NAFLD was 30%. We identified five a posteriori dietary patterns (cumulative explained variation [R2] = 20%). The patterns were characterised as: vegetable and fish, red meat and alcohol, traditional, salty snacks and sauces, high fat dairy & refined grains pattern. Adherence to the traditional pattern (i.e. high intake of vegetable oils/stanols, margarines/butters, potatoes, whole grains and sweets/desserts) was associated with regression of NAFLD per SD increase in Z-score (0.40, 95% CI 0.15–1.00). Adherence to the three a priori patterns all showed regression of NAFLD, but only the WHO-score showed a distinct association (0.73, 95% CI 0.53–1.00). Hence, in this large elderly population, adherence to a plant-based, high-fibre and low-fat diet was related to regression of NAFLD