Atomic-Number (Z)-Correlated Atomic Sizes for Deciphering Electron Microscopic Molecular Images

With the advent of atomic-resolution transmission electron microscopy (AR-TEM) achieving sub-{\AA}ngstrom image resolution and submillisecond time resolution, an era of visual molecular science where chemists can visually study the time evolution of molecular motions and reactions at atomistic precision has arrived. However, the appearance of experimental TEM images often differs greatly from that of conventional molecular models, and the images are difficult to decipher unless we know in advance the structure of the specimen molecules. The difference arises from the fundamental design of the molecular models that represent atomic connectivity and/or the electronic properties of molecules rather than the nuclear charge of atoms and electrostatic potentials that are felt by the e-beam in TEM imaging. We found a good correlation between the atomic number (Z) and the atomic size seen in TEM images when we consider shot noise in digital images. We propose here Z-correlated (ZC) atomic radii for modeling AR-TEM images of single molecules and ultrathin crystals, with which we can develop a good estimate of the molecular structure from the TEM image much more easily than with conventional molecular models. Two parameter sets were developed for TEM images recorded under high-noise (ZCHN) and low-noise (ZCLN) conditions. The new molecular models will stimulate the imaginations of chemists planning to use AR-TEM for their research.Comment: 27 pages, 6 figure

Screening of Leuconostoc Strains with Antibacterial Activity from Fermented Vegetables and Mining of Bacteriocin Gene Cluster

In order to improve the safety and preservability of fermented vegetables, strain Leuconostoc mesenteroides AP7, with potent inhibitory effects on foodborne pathogens and bacteria causing paocai over-acidification, was selected from 8 Leuconostoc strains derived from traditional fermented vegetables in Yunnan, China. The main antimicrobial substance of AP7 was determined by eliminating the effects of organic acid and H2O2 and through protease sensitivity. The acid stability and thermal stability were analyzed and potential bacteriocin gene clusters were mined from the whole genome sequence of AP7. The results showed that the culture supernatant still had obvious antibacterial activity after excluding the influence of acid and H2O2, and after protease treatment, the antibacterial effect decreased significantly. It was speculated that the antibacterial substance in the concentrated culture supernatant was bacteriocin. The bacteriocin was sensitive to pH changes, had high thermal stability, and had a molecular weight of 6.51~14.4 kDa. Whole genome sequencing showed that the whole genome of AP7 contained 1 chromosome (1948310 bp) and 2 plasmids (37366 and 20698 bp), with a GC content of 37.7%. There was a gene cluster with Enterocin_X_chain_beta bacteriocin as the core. Its encoded product was predicted to be a positively charged hydrophilic stable protein, the secondary structure was dominated by α-helix, and the tertiary structure was mainly composed of loose peptide chains at both ends and an α-helix in the middle. In summary, the bacteriocin-producing Leuconostoc mesenteroides AP7 has excellent antibacterial properties and has the potential to be applied to the fermentation and preservation of acidic foods

Thermo-responsive fluorescent vesicles assembled by fluorescein-functionalized pillar[5]arene

Fluorescent vesicles were successfully prepared through the self-assembly of a fluorescein-functionalized pillar[5]arene. The vesicular superstructure exhibits thermo-responsive emission under variable temperatures

Relationships between the Visual Quality and Color Patterns: Study in Peri-Urban Forests Dominated by <i>Cotinus coggygria</i> var. <i>cinerea</i> Engl. in Autumn in Beijing, China

The spatial pattern of color patches plays a crucial role in affecting the visual quality of peri-urban forests dominated by Cotinus coggygria var. cinerea Engl. in autumn. The impact mechanism has been studied to facilitate algorithm-based automatic visual quality estimation. The color patterns of 120 photographs were calculated after color quantization and automatic color substitution. The scenic beauty of the forest was estimated by 698 respondents. Multiple correlations between visual quality and color pattern metrics were explored with stepwise regression. Principal component analysis (PCA) was also employed to investigate the impact mechanism of color patterns on visual quality. Number of patches (NP), largest patch index (LPI), mean patch area (AREA_MN), patch size standard deviation (AREA_SD), and Shannon’s evenness index (SHEI) were the main factors affecting the visual quality of the Cotinus coggygria forest. AREA_MN correlated positively with visual quality, while NP, LPI, AREA_SD, and SHEI correlated negatively. Moreover, AREA_SD had the most significant impact on the visual quality of the landscape, while SHEI, LPI, and AREA_MN had the second-highest impact. The evenness and the size of color patches significantly affected the visual quality of the forest landscapes. Balancing the diversity and evenness of color patches plays a decisive role in creating a forest landscape with high visual quality

Associations between maternal occupational exposures and pregnancy outcomes among Chinese nurses: a nationwide study

Abstract Background Several studies have provided evidence about adverse pregnancy outcomes of nurses involved in occupational exposure. However, the pregnancy outcomes among nurses in middle-income countries are not well demonstrated. The main aim of this study is to present the prevalence and influencing factors of pregnancy outcomes among female nurses in China. Methods We included 2243 non-nurse health care workers, and 4230 nurses in this national cross-sectional study in China. Information on occupational exposures and pregnancy outcomes was collected using a face-to-face investigation. Odds ratios (ORs) were estimated through logistic regression. Results The proportion of threatened abortion, spontaneous abortion, and stillbirth of female nurses was 2.6%, 7%, and 2.1%, respectively. We found an increased risk of threatened abortion among nurses with overtime work (OR = 1.719, 95% CI 1.158–2.550). The risk of threatened abortion and spontaneous abortion was elevated among nurses handling disinfectant (OR = 2.293 and 1.63, respectively). We found a nearly twofold increased risk of premature birth (OR = 2.169, 95% CI 1.36–3.459) among nurses handling anti-cancer drugs. Conclusions Our findings suggested that maternal occupational exposures might be associated with the risk of adverse pregnancy outcomes among female nurses in China. We recommend that policy-markers and hospital managers work together to reduce exposure to occupational hazards and improve pregnancy outcomes among female nurses