KECERNAAN NUTRIEN PAKAN TEPUNG DAUN MURBEI PADA SAPI PERANAKAN ONGOLE

The  research was conducted to study the ability of mulberry leave meal addition concentrates on the digestibility  nutrient  of Ongole Grade. This experiment used a randomized block design, with 4 treatments and 4 blocks. Treatments consisted of P1 (native grass and concentrate feed in the form of mulberry leaves meal and concentrate complete), P2 (native grass and concentrate feed in the form of mulberry leaves meal and corn concentrate), P3 (native grass and concentrate feed in the form of mulberry leaves meal and rice bran concentrate), P4 (native grass and concentrate feed in the form of mulberry leaves meal and concentrate tapioca waste). The experiment was conducted for 4 months with the adaptation periods for 2 weeks. Parameters measured were digestibility of dry matter (KCBK) and organic matter (KCBO), . The results showed that the mulberry leave meal addition on different concentrate did  significantly (P<0,05) on digestibility of dry matter (KCBK) and organic matter (KCBO). It is concluded that based on the nutrient digestibility, mulberry leave meal concentrate can be combined with a single concentrate such as corn, cassava waste meal or complete concentrate. Keywords: mulberry leave meal, ongole grade cattle, digestibility of dry matter  and organic matter

Selection of Lactic Acid Bacteria as Probiotic Candidate for Chicken

Lactic acid bacteria (LAB) regarded as safe microorganisms; they can naturally live in gastrointestinal tract, so appropriately used as a probiotic for chicken. This study aimed to select six isolates of LAB (E1223, E3, E4, E5, E7, and E8) to obtain the isolates potentially as probiotic candidate for chicken. The six isolates were derived from spontaneous fermented corn obtained from Laboratory of Animal Biotechnology and Biomedical, PPSHB, Bogor Agricultural University, Indonesia. LAB isolates were tested their susceptibility to antibiotics (bambermycin, erythromycin, chloramphenicol, and tetracycline) then were examined in vitro for their tolerance to gastrointestinal pH (2, 3, 4, and 7.2) and 0.5% bile salt condition, antimicrobial activity against Salmonella enteritidis and Enterococcus casseliflavus, and ability to adhere to chicken ileal cells. The results showed the isolates E5, E7, and E8 were sensitive to tetracycline and chloramphenicol, they could survive at pH 2, 3, 4, and 7.2, could survive at 0.5% bile salts, produced antimicrobial activity, and able to adhere to ileal cells (9.40±0.00 Log CFU/cm2 of E8) and were significantly (P&lt;0.05) higher than those of control (5.30±0.14 Log CFU/cm2). In conclusion, this study showed that isolate E8 had better potential compared to isolates E5 and E7 in most in vitro assays as a probiotic candidate for chicken. E5, E7, and E8 were closely related with Pediococcus pentosaceus based on 16S rRNA gene.Key words: LAB, probiotic, chicken, in vitr

Synergistic Effect of Biofat and Biochar of Cashew Nutshell on Mitigate Methane in the Rumen

The study aimed to evaluate the effectiveness of a combination of biofat with biochar or biosmoke (bioindustrial products of cashew nut shells) at the best level as feed additive in reducing methane production and improving in vitro rumen fermentation. This experiment had two series of combination and each used a randomized block design with 6 treatments and 4 replications. A series of biofat (BF) and biochar (BC) combination were added each to substrate as followed BFBC1 = 0: 100%; BFBC2 = 25:75%; BFBC3 = 50:50%; BFBC4 = 75:25%; BFBC5 = 100: 0%. While, a series of biofat (BF) and biosmoke (BS) combination as followed BFBS1 = 0: 100%; BFBS2 = 25:75%; BFBS3 = 50:50%; BFBS4 = 75:25%; BFBS5 = 100: 0%. Both series used a control treatment which contained only substrate. The in vitro experiment was repeated 4 times and each treatment was done in duplicates. The measured variables were: total gas and CH4 productions, dry matter, organic matter, NDF degradability, NH3 and partial VFA concentrations. The results showed that the combination of biofat and biochar levels resulted in a significant decrease (

The effect of combined probiotics with catalyst supplementation on digestion and rumen characteristic in Priangan sheep

An experiment was carried out to investigate the effect of combined supplementation of probiotics and catalyst on digestion and rumen characteristic in Priangan sheep. The trial was conducted using 16 heads of young male Priangan sheep with average initial weight of 18 kg in completely randomized design with factorial 2x2 and 4 replication. The first factor was two types of probiotics mixed with catalyst supplement, while the second factor was two levels of supplement percentage of catalyst at 0.5 and 1.0% of concentrate. The type of probiotics applied was probion and probiotics of buffaloes rumen microbes. The feeding level was set at 3% of body weight based on dry matter and consisting of forage (King grass) and concentrate at 50:50 ratio. The results indicated a significantly greater fibre digestion value (NDF) and proportion of acetate molar in the group of sheep fed combination of probiotics of buffaloes rumen microbes and catalyst supplement. It was concluded that the recommendation level of the combined rumen microbe probiotics with catalyst supplement in sheep ration was 0.5%. Key words: Probiotic-Catalyst Supplement, Digestibiliy, Rumen Characteristic, Shee

Total Digestible Nutrient of Diet Containing PUFA- Concentrate Supplemented with Yeast and Curcuma Xanthorrhiza Roxb for Dairy Goat

Penelitian ini didisain untuk mengevaluasi kecernaan nutrisi, dinyatakan dalam TDN, dari ransum yang mengandung PUFA konsentrat yang disuplementasi yeast dan Curcuma xanthorrhiza Roxb pada kambing perah. Dua puluh ekor kambing Peranakan Ettawah (PE) pada fase laktasi akhir dialokasikan kedalam 6 perlakuan dengan Rancangan Acak Kelompok. Perlakuan tersebut adalah: PD0: PUFA-diet tanpa suplemen, PDA: dengan Asifit, PDY: dengan 0,5% yeast, PDC: dengan 2% curcuma, dan PDM: dengan 0,5% yeast + 2% curcuma. Ransum- PUFA terdiri atas 80% PUFA- konsentrat sebagai ransum dasar dan 20% rumput gajah. Sumber PUFA terdiri atas jagung giling sangrai, tepung kedelai sangrai, dan minyak jagung. Hasil menunjukkan bahwa konsumsi DM, OM, CP, CF, NFE, ADF, dan GE tidak terpengaruh oleh perlakuan. Namun, EE dan NDF antarperlakuan berbeda signifikan (P&lt;0.05), sedang Ca dan P berbeda sangat signifikan (P&lt;0.01). Kandungan nutrisi yang tinggi (DM, OM, CP, EE, CF, NDF, and GE) ditemukan di feces pada kambing dengan perlakuan Curcuma (PDC), konsekuensinya adalah kecernaan nutrisinya secara signifikan (P&lt;0.05) atau sangat signifikan (P&lt;0.01) terendah. Total digestible nutrient (TDN) dari PDY dan PDM secara signifikan tinggi (79,89% dan 79,37%) disbanding ransum lainnya, terendah pada PDC (69,94%). Hal ini menunjukkan bahwa yeast atau ragi dengan konsentrasi 1,8 108cfu/d baik disuplementasikan tunggal atau dikombinasi dengan 2% Curcuma dapat dikatakan sebagai kandungan yang tepat untuk memperbaiki kecernaan nutrisi pada kambing perah dengan laktasi akhir pada penelitian ini

Total Digestible Nutrient of Diet Containing PUFA- Concentrate Supplemented with Yeast and Curcuma Xanthorrhiza Roxb for Dairy Goat

Penelitian ini didisain untuk mengevaluasi kecernaan nutrisi, dinyatakan dalam TDN, dari ransum yang mengandung PUFA konsentrat yang disuplementasi yeast dan Curcuma xanthorrhiza Roxb pada kambing perah. Dua puluh ekor kambing Peranakan Ettawah (PE) pada fase laktasi akhir dialokasikan kedalam 6 perlakuan dengan Rancangan Acak Kelompok. Perlakuan tersebut adalah: PD0: PUFA-diet tanpa suplemen, PDA: dengan Asifit, PDY: dengan 0,5% yeast, PDC: dengan 2% curcuma, dan PDM: dengan 0,5% yeast + 2% curcuma. Ransum- PUFA terdiri atas 80% PUFA- konsentrat sebagai ransum dasar dan 20% rumput gajah. Sumber PUFA terdiri atas jagung giling sangrai, tepung kedelai sangrai, dan minyak jagung. Hasil menunjukkan bahwa konsumsi DM, OM, CP, CF, NFE, ADF, dan GE tidak terpengaruh oleh perlakuan. Namun, EE dan NDF antarperlakuan berbeda signifikan (P&lt;0.05), sedang Ca dan P berbeda sangat signifikan (P&lt;0.01). Kandungan nutrisi yang tinggi (DM, OM, CP, EE, CF, NDF, and GE) ditemukan di feces pada kambing dengan perlakuan Curcuma (PDC), konsekuensinya adalah kecernaan nutrisinya secara signifikan (P&lt;0.05) atau sangat signifikan (P&lt;0.01) terendah. Total digestible nutrient (TDN) dari PDY dan PDM secara signifikan tinggi (79,89% dan 79,37%) disbanding ransum lainnya, terendah pada PDC (69,94%). Hal ini menunjukkan bahwa yeast atau ragi dengan konsentrasi 1,8 108cfu/d baik disuplementasikan tunggal atau dikombinasi dengan 2% Curcuma dapat dikatakan sebagai kandungan yang tepat untuk memperbaiki kecernaan nutrisi pada kambing perah dengan laktasi akhir pada penelitian ini

Fermentation Characteristics and Nitrogen Retention of Madura Cattle Fed Complete Rations Containing Soybean Pod and By-Products

This study was aimed to evaluate the effect of complete rations containing soybean pod and soybean by-products (soybean meal and tofu waste) on rumen microbial population, fermentation characteristics, nutrient digestibility, and nitrogen retention of Madura cattle. Twelve Madura cattle of 1.5 years of age were given 4 feeding treatments in triplicates in randomized block design experiment. The treatments included T0 (100% native grass) as a negative control, T1 (concentrate: grass (60:40) as a positive control, T2 (complete ration containing 15% soybean pods), and T3 (complete ration containing 30% soybean pods). The treatments were based on feeding practices commonly applied by farmers in the village. The results showed that the use of concentrate rations or complete rations containing soybean pod and by-product did not affect protozoa population, ammonia concentration, and total VFA production compared to cattle fed 100% native grass. In contrast, the use of concentrate rations or complete rations containing soybean pod and by-products reduced acetate and increased butyrate proportion compared to native grass. The use of a concentrate ration resulted the highest propionate proportion. Methane estimation increased with the use of concentrate ration or complete ration containing 15% soybean pod, but it decreased when the level of soybean pod was increased to 30%. It can be concluded that soybean pod has a potential to be used as a fiber source in beef cattle ration to substitute native grass

Pengujian secara In Vitro Oligosakarida dari Ekstrak Tepung Buah Rumbia (Metroxylon sago Rottb.) sebagai Sumber Prebiotik

In vitro analysis of oligosaccharide from extract rumbia fruit (Metroxylon sago Rottb.) as prebioticABSTRACT. Despite a range of commercially available oligosaccharides there is plenty of room to develop new, functionally enhanced prebiotics. current generation of oligosaccharides was not rationally developed. better understanding of factors determining the prebiotic activity of a particular oligosaccharide. Despite the range of commercially available oligosaccharides mixtures (mainly fructo and galacto-oligosaccharides), very few studies are focused on the mechanisms behind the prebiotic activity of particular oligosaccharides. Probably this lack is due to the unavailability of well characterized oligosaccharide fractions for prebiotic function assessment. The objectives of this research were to asses the ability of lactic acid bacteria in fermentation of oligosaccharide and as prebiotic (in vitro). Material used was oligosaccharide of purified rumbia fruit extract. Analysis of oligosaccharide as prebiotic was conducted in vitro using lactic acid bacteria. The lactic acid bacteria consisted Bifidobacterium bifidum, Bifidobacterium animalis, Lactobacillus bulgaricus and Lactobacillus casei Rhamnosus. The growth media for bacteria was a liquid MRS basic medium where glucose was substituted by oligosaccharide of purified rumbia fruit extract. Incubation was in aerob for Lactobacillus and anaerob for Bifidobacterium in incubator 37oC. The lactic acid bacteria was calculated 24-48 hours during incubation periode. The variables observed were: oligosaccharide component, ability of lactic acid bacteria in fermentation of oligosaccharide, and growth of lactic acid bacteria (Lactobacillus and Bifidobacterium). The result showed that the oligosaccharide component from extract rumbia fruit consisted of: sucrose, stacchiose, and raffinose. The result showed that the oligosaccharide extract rumbia fruit was significantly (P0.05) the growth of lactic acid bacteria (Lactobacillus and Bifidobacterium) and fermentation of oligosaccharide. It is concluded that oligosaccharide of rumbia fruit extract could be used as prebiotic

PROFIL LEMAK PLASMA DAN NILAI HEMATOLOGI TIKUS SPRAGUE DAWLEY DENGAN SUPLEMENTASI AMIDA MINYAK IKAN

Penelitian ini bertujuan mengetahui efektivitas amida minyak ikan dalam menurunkan konsentrasi kolesterol dan trigliserida plasma dan pengaruhnya terhadap nilai hematologi darah. Tiga puluh lima ekor tikus jantan, galur Sprague Dawley, umur 7 minggu dibagi secara acak ke dalam lima kelompok perlakuan. Kelompok kontrol (A) mendapat ransum semi purified yang mengandung 8% minyak jagung. Kelompok perlakuan masing-masing disuplementasi 4,5% minyak ikan (B), 3% minyak ikan+1,5% amida (C), 1,5% minyak ikan+3% amida (D), dan 4,5% amida (E). Ransum perlakuan diberikan selama 6 minggu. Pada akhir penelitian dilakukan pengambilan sampel darah untuk menganalisis nilai hematologi dan profil lemak plasma. Hasil penelitian menunjukkan bahwa suplementasi amida minyak ikan dapat mempertahankan jumlah eritrosit dan Hb, namun nilai hematokrit mulai menurun pada suplementasi amida 3% (D), dibandingkan suplementasi minyak ikan (B) dan suplementasi amida 1,5% (C). Jumlah leukosit pada kelompok yang mendapat suplementasi 4,5% amida (E) secara nyata (P0,05) lebih tinggi dibandingkan dengan kelompok yang diberi minyak ikan (B). Suplementasi amida minyak ikan tidak berpengaruh terhadap konsentrasi kolesterol total dan HDL plasma, namun pada suplementasi 3% amida mulai meningkatkan konsentrasi trigliserida dan LDL plasma secara nyata (P0,05). Disimpulkan bahwa suplementasi amida sebesar 3% mulai memperlihatkan pengaruh negatif terhadap nilai hematologi dan profil lemak plasma

Penggunaan Prebiotik Oligosakarida Ekstrak Tepung Buah Rumbia (Metroxylon sago Rottb.) dalam Ransum terhadap Performan Ayam Pedaging

Effect of prebiotic oligosaccharide extract rumbia fruit (Metroxylon sago Rottb.) in the ration on broiler performanceABSTRACT. Prebiotic oligosaccharides are thought to provide beneficial effects in the gastrointestinal tract of humans and animals by stimulating growth of selected members of the intestinal microflora. Prebiotic oligosaccharides are defined as nondigestible food ingredients that provide beneficial effects to the host by stimulating the growth of selected microbial members of the gastrointestinal tract. Among the colonic bacteria capable of metabolizing prebiotic oligosaccharides and whose growth is stimulated are species of Lactobacillus and Bifidobacterium. Prebiotic oligosaccharides can be produced in transgli-cosylation reactions catalyzed by glycosidases. Glycosidases from different biological sources have specific ability to catalyze the formation of oligosaccharides with particular chain lengths (usually DP 7) and predominant glycosidic linkages. Oligosaccharide used this research was purified rumbia fruit extract as prebiotic for feed additive in the ration on broiler. The objectives of this research were to study the performance of broiler given of prebiotic oligosaccharide extract rumbia fruit (metroxylon sago Rottb.) in the ration. Two hundred day-old chicks of broiler were divided into three dietary treatments and four replications. Ration used was consisted of: R1 = basal ration (control), R2 = basal ration + 0,4% oligosaccharide extract rumbia fruit, and R3 = basal ration + 0,4% frukto-oligosaccharide (FOS). The variables observed were: feed consumption, body weight, feed conversion ratio, mortality, and production index. The result showed that the performances of the broiler supplemented prebiotic oligosaccharide extract rumbia fruit (R2) was significantly (P 0.05) differences between of feed consumption, body weight, and mortality. It is concluded that of prebiotic oligosaccharide extract rumbia fruit were able to decrease the mortality and feed consumption at six week of age