CELLULAR MECHANISM UNDERLYING EMBRYO-MATERNAL RELATIONSHIP IN INTRASPECIFIC AND INTERSPECIFIC PREGNANCY

Mouse and vole embryos were transferred into pseudopregnant CD-1 and scid female mice. Cellular changes involved in the formation of decidua in the pregnant mouse uterus up to day 8 of pregnancy were examined by histological, electron microscopic, and histochemical techniques. On day 6 of pregnancy, the vole embryos were laid in interstitium of antimesometrial side of the uterus, as well as in intraspecific pregnancy. Compared with intraspesific pregnant mouse, blood vessels were numerous in the decidua around the vole embryos in interspecific pregnancy. Both distribution and dilation of the blood vessels were increased on day 8. A part of cells in the inner cell mass had not nuclei, suggesting damaged vole embryos on day 8. At the implantation site, the uterine decidua was invaded by extravillous trophoblast (EVT) cells whose function is to destroy the walls of the uterine spiral. Moreover, this decidua was infiltrated by a population of natural killer (NK) cells and macrophage. These cells were particularly numerous in the decidua basalis at the implantation site where they come into close contact with invading EVT cells. These results suggest that interaction between NK, macrophage, and EVT provides the controlling relationship of embryo-maternal in intraspecific and interspecific pregnancy

Pengaruh Ukuran Folikel terhadap Qualitas Oosit Sapi Peranakan Ongole dan Kemampuan Maturasi In Vitro

Artikel dalam bentuk PD

Pengaruh Ukuran Folikel Terhadap Qualitas Oosit Sapi Peranakan Ongole Dan Kemampuan Maturasi In Vitro

INTISARI Penelitian ini bertujuan untuk mengetahui pengaruh ukuran folikel terhadap kualitas oosit sapi Peranakan Ongole dan kemampuan maturasi in vitro. Ovaria diambil dari rumah potong hewan segera sesudah sapi disembelih dan dibawa ke laboratorium dalam NaCI 0,9%, 31-34°C. Oosit diaspirasi dan folikel yang berukuran 2,00-4,50 mm dan 4,51-7,00 mm dengan menggunakan siring 5 ml dengan jarum 19G yang telah diisi 1 ml Dulbecco\u27s phosphate buffered saline (D-PBS) + 3% new born calf serum (NBCS). Pencarian oosit dilakukan dibawah mikroskop stereo. Oosit dicuci dengan D-PBS + 3% NBCS sebanyak dua kali kemudian dicuci dengan media maturasi yang terdiri dan hepes-buffered tissue culture medium 199 (TCM 199) dengan Earle\u27s salt yang ditambah dengan penisilin G 100 IU/rni dan streptomisin sulfa! 10mWm1 + 5% NBCS. Oosit dipisahkan kedalam dua kelompok, yaitu kelompok folikel kecil (diameter 2,00-4,50 mm) dan kelompok folikel besar (diameter 4,51-7,00 mm). Maturasi oosit in vitro dilakukan dengan care meletakkan oosit dalam 100n1 media maturasi yang ditutup dengan minyak mineral dan dimasukkan dalam inkubator CO2 pada suhu 39°C dan kadar CO2 5% selama 22 jam. Variabel yang diamati meliputi qualitas oosit dan angka maturasi. Data dianalisis secara statistik menggunakan chi-square. Hasil penelitian ini menunjukkan terdapat perbedaan yang nyata pada kualitas oosit. Kualitas oosit untuk kelompok folikel kecil dan folikel besar masing-masing adalah kelas I (kompak) 45,14% dan 58,53%, kelas II (dikelilingi sel-sel kumulus yang tidak utuh) adalah 34,72% dan 18,18%, kelas IH (denuded) 18,06% dan 11,36% serta kelas IV (dikelilingi fibrin) adalah 2,08% dan 11,93%. Hasil penelitian juga menunjukkan angka maturasi kelompok fakel kecil dan kelompok folikel besar secara statistik berbeda nyata Angka maturasi untuk masing-masing kelompok folikel adalah 79,16% dan 86,93%. Berdasarkan basil penelitian dapat disimpulkan bahwa ukuran folikel berpengazuh terhadap kualitas oosit sapi Peranakan Ongole dan kemampuan maturasi in vitro. (Katy Kunci: Ukuran Folikei, Kualitas Oosit, Sapi Peranakan Ongole, Maturasi In Vitro)

Supplementation of Follicle Stimulating Hormon Into In vitro Maturation Medium to Increase Oocytes Maturation and 4 Cell Stadium Embryo Development of Bligon Goat

The study was carried out to investigate the effect of follicle stimulating hormon (FSH) into in vitro maturation medium to increase oocytes maturation and 4 cell stadium embryo development of Bligon goat. Goat ovaries were obtained from a slaughterhouse and transported to the laboratory in a flask of NaCl at temperature of 31 – 34°C. Oocytes were aspirated from 2 – 6 mm of follicles into a 3 mL syringe (23G needle) that contained Dulbecco’s Phosphate-Buffered Saline. Oocytes were divided into three groups, i.e tissue culture medium (TCM) with FSH supplementation 0, 50, and 100 IU/mL. Oocytes were put into those medium and incubated on 39°C, 5% CO2, and 95% humidity for 24 hours. Matured oocytes were fertilized with capacitated frozen thawed-semen and incubated on 39°C, 5% CO2, and 95% humidity for 5 hours. Fertilized oocytes were washed for 3 times in TCM and incubated in the same condition for embryo culture. The data of FSH supplementation and embryo development were analyzed using randomized completely one way classification. The results showed that the percentages of mature oocytes from FSH supplementation 0, 50, and 100 IU/mL were 70,48±23,22, 78,48±15,80, and 80,29±12,86%, respectively. Cleavage rate of the two cells stage were 36,00±14,22, 44,00±33,94, and 57,45±31,78%, respectively, and for the 4 cells stage were 27,33±22,04, 35,33±40,73, and 39,45±20,38%. It is concluded that supplementation of FSH in the maturation medium could not increase the percentages of in vitro maturation and embryo development

The Effect of Thawing Duration on the Post Thawing Quality of Bali Cattle’s Frozen Semen and Conseption Rate in Smallholder Farms of East Lombok Regency

This research aimed to identify the effect of the length of thawing time on the quality  of  Bali  cattle postthaw semen and  to  investigate  the resulting pregnancy  rate. This  research  was  conducted  at  Wanasaba  Village,  specifically  Tanaq  Mira  Village, Wanasaba  District,  East  Lombok  Regency.  Five  samples  of  Bali  cattle  semen  were  collected  for  insemination  from  each  of  three  different  farmer  groups.  The  semen sample  from  the  remaining  frozen  semen  inseminated  by  the  inseminator  was  used  in this research. Tanaq Mira Village's inseminator performed thawing during the trip from the  artificial  insemination  (AI)  station  to  the  farmer  group's  location.  This  research observed  the  microscopic  quality  of  the postthaw frozen  semen  covering  motility, viability, and abnormality. Moreover, the pregnancy rate on AI acceptors using the non return  rate (NRR)  parameter  or  the  number  of  female parents who returned to  estrus after being inseminated were observed. The observation was conducted on three farmer groups  with  different  distances  and  lengths  of  thawing  time.  Each  farmer  group  was observed five times. The collected data were then analysed by using one-way analysis of variance(ANOVA). The research indicated that the distance of the farmer group’s location significantly affected (P0,05) the sperm abnormalities. The Sapeng farmer group had the  highest NRR, as  much  as 80%. According  to the  NRR  score,  it  can  be  concluded that the thawing process  using  the  thermos  within  less  than 10 minutes  resulted  in  the best yield

The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours

In order to increase the livestock population, a good reproductive process was needed. Lycopene which belongs to carotenoid group has a dark yellow pigment. This study aimed to determine the effect of adding lycopene on the semen quality of Saanen goats. The research design used 4 treatments, P0 (egg yolk tris diluent) as control), P1 (egg yolk tris diluent + 2% lycopene), P2 (egg yolk tris diluent + 4% lycopene), and P3 (yolk tris diluent). egg + 6% lycopene). The quality of semen was observed after 24 hours of storage at 5℃. Macroscopic data were analyzed descreptively and the microscopic one was analyzed using a completely randomized design (CRD). The results showed that the addition of lycopene in egg yolk tris diluent had a significant (P<0.05) effect on the semen quality of Saanen goats. The results showed that the motility of spermatozoa in each treatment group (P0, P1, P2, and P3) was 60.1 ± 5.7%, 65.2 ± 4.3%, 62.4 ± 5.2%, and 60.6 ± 4.7%, respectively, viability were 65.3 ± 4.8, 73.5 ± 4.2, 70.4 ± 3.8, and 66.1 ± 5.1, respectively, abnormality were 17 ± 1.8%, 16 ± 1.8%, 18 ± 1.9%, and 16 ± 1.7%, respectively, and membrane integrity were 63 ± 3.3%, 70 ± 3.6 %, 67 ± 3.2%, and 62 ± 2.9%, respectively. Based on the results, it could be concluded that the lycopene addition improved the semen quality of Saanen goats compared to controls

On Stein's equation, Vandermonde matrices and Fisher's information matrix of time series processes. Part I: The autoregressive moving average process

AbstractThis paper introduces several forms of relationships between Fisher's information matrix of an autoregressive-moving average or ARMA process and the solution of a corresponding Stein equation. Fisher's information matrix consists of blocks associated with the autoregressive and moving average parameters. An interconnection with a solution of Stein's equation is set forth for the block case as well as for Fisher's information matrix as a global matrix involving all parameter blocks. Both cases have their importance for the interpretation of the estimated parameters. The cases of distinct and multiple eigenvalues are addressed. The obtained links involve equations with left and right inverses, these can be expressed in terms of the inverse of appropriate Vandermonde matrices. A condition is set forth for establishing an equality between Fisher's information matrix and a solution to Stein's equation. Two examples are presented for illustrating some of the results obtained. The global and off-diagonal block case with distinct and multiple roots, respectively, are considered

EVALUATION OF FRIESIAN HOLSTEIN GRADE COWS FERTILITY BASED THE LEVEL OF MILK UREA

This study was conducted to evaluate fertility grade of Friesian Holstein cows based on milk urea levels. A number of eight cows which fulfill the criteria of not pregnant and in second to sixth lactation, produced milk of 2.5 liters/day, and body weight ranging from 400-450 kg were used in this research. The cows were given fodder that consisted of 30 kg of forage and 10 kg of a mixture of concentrate (pollard, pulp out, and molasses), in the morning and afternoon. Milk and blood samples were taken once a week for five weeks in the morning before fed. Milk urea levels were measured using the urea FS (DiaSys) and blood samples were tested using enzyme linked immuno-sorbent assay (ELISA) with the progesterone hormone kit (DRG, Germany). The variables measured were pregnancy rate, service per conception (S/C), days open, and progesterone levels. Reproduction and milk urea levels data were analyzed by linear regression to determine the relationship between two variables. Progesterone data were analyzed descriptively. The results showed that the average calving interval, days open, and S/C were 607.5±170.27 days; 341.25±187.65 days; and 2.41±2.32 days, respectively. The average of milk urea levels was 14.16±2.55 mg/dL. Regression analysis showed a significant effect (P0.05) and negative correlation between milk urea level and S/C. The average of non-pregnant progesterone hormone level was 12.04±7.13 ng/mL. In conclusion, the fertility grade of Friesian Holstein cows decreased when the milk urea level at 14.16 mg/dL and non-pregnant progesterone at 12.04±7.13 ng/mL

Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon

The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM) with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality

CORRELATION BETWEEN BLOOD METABOLITE AND REPRODUCTIVE PERFORMANCE OF LACTATING HOLSTEIN FRIESIAN CROSSBRED COWS IN SMALLHOLDER FARMERS

This study examined the correlation between the concentration of blood metabolites (triglyceride, albumin, phosphorous) on the reproductive performances (S/C and PPM) of lactating Holstein Friesian crossbred (HFC) cows. Blood samples were collected through the caudal vein 8 hours after feeding and the plasma was analyzed at the Integrated Research and Testing Laboratory of Universitas Gadjah Mada (LPPT UGM). Samples were stored at 5° C. Triglyceride, albumin, and phosphorous were measured using glucose oxidase-phenol 4-aminoantipyrine (GOD-PAP) method, photometric bromocresol green method, and cypress diagnostic, respectively. Blood metabolites data were analyzed using Pearson correlation model. The results showed that the correlation coefficients of triglycerides, albumin, and phosphorus levels on PPM and S/C were -0.521 and -0.650; -0.447 and -0.612; -0.513 and -0.700, respectively. In conclusion, there are significant negative correlation between triglyceride, albumin and phosphorus levels on the PPM, and very significant negative correlation between triglyceride, albumin and phosphorus levels on the S/C of lactating HFC cows