Vine shoots pre-treatment strategies for improved hydrogen production and metabolites redistribution in Clostridium butyricum

This work deals with the use of vine shoots, a renewable, largely available, lacking of alternatives lignocellulosic material as a feedstock for hydrogen production. Physical pre-treatments by steam explosion (SE), chemical by organosolv (OS) and biological by laccase (LAC) were carried out in vine shoots to disrupt the cell fiber and increase the biomass hydrolysis and fermentation into hydrogen (H2). After SE, there was a slight decrease in cellulose and hemicellulose contents in biomass fibers, while a decrease in lignin content occurred after OS pretreatment. There were no quantifiable changes after laccase pre-treatment, however the enzyme-substrate oxidative reactions were favorable for hydrolysis and fermentation since an increase in soluble sugars and H2 production was observed with LAC vine shoots as substrate. 300.1 mL H2/L were obtained from raw material vine shoots, while 649.4, 399.8 and 749.7 mL H2/L were obtained from biomass pre-treated by SE, OS and LAC, respectively. Furthermore, the hydrolysis of pre-treated biomass by addition of cellulase was evaluated to improve H2 production. Higher amounts of H2 were obtained from hydrolyzed biomass in relation to nonhydrolyzed ones (154.2%, 602.0% and 167.1% more with SE, OS and LAC hydrolyzed, respectively). In all cases, the mixed acid pathway was carried out by Clostridium butyricum, since acetic and butyric acids were produced.MCIN/AEI/10.13039/501100011033/ FEDER, reference project PID2020-112594RB-C31. Junta de Andalucía, Postdoctoral researcher R-29/12/202

Contex-aware gestures for mixed-initiative text editings UIs

This is a pre-copyedited, author-produced PDF of an article accepted for publication in Interacting with computers following peer review. The version of record is available online at: http://dx.doi.org/10.1093/iwc/iwu019[EN] This work is focused on enhancing highly interactive text-editing applications with gestures. Concretely, we study Computer Assisted Transcription of Text Images (CATTI), a handwriting transcription system that follows a corrective feedback paradigm, where both the user and the system collaborate efficiently to produce a high-quality text transcription. CATTI-like applications demand fast and accurate gesture recognition, for which we observed that current gesture recognizers are not adequate enough. In response to this need we developed MinGestures, a parametric context-aware gesture recognizer. Our contributions include a number of stroke features for disambiguating copy-mark gestures from handwritten text, plus the integration of these gestures in a CATTI application. It becomes finally possible to create highly interactive stroke-based text-editing interfaces, without worrying to verify the user intent on-screen. We performed a formal evaluation with 22 e-pen users and 32 mouse users using a gesture vocabulary of 10 symbols. MinGestures achieved an outstanding accuracy (<1% error rate) with very high performance (<1 ms of recognition time). We then integrated MinGestures in a CATTI prototype and tested the performance of the interactive handwriting system when it is driven by gestures. Our results show that using gestures in interactive handwriting applications is both advantageous and convenient when gestures are simple but context-aware. Taken together, this work suggests that text-editing interfaces not only can be easily augmented with simple gestures, but also may substantially improve user productivity.This work has been supported by the European Commission through the 7th Framework Program (tranScriptorium: FP7- ICT-2011-9, project 600707 and CasMaCat: FP7-ICT-2011-7, project 287576). It has also been supported by the Spanish MINECO under grant TIN2012-37475-C02-01 (STraDa), and the Generalitat Valenciana under grant ISIC/2012/004 (AMIIS).Leiva, LA.; Alabau, V.; Romero Gómez, V.; Toselli, AH.; Vidal, E. (2015). Contex-aware gestures for mixed-initiative text editings UIs. Interacting with Computers. 27(6):675-696. https://doi.org/10.1093/iwc/iwu019S675696276Alabau V. Leiva L. A. Transcribing Handwritten Text Images with a Word Soup Game. Proc. Extended Abstr. Hum. Factors Comput. Syst. (CHI EA) 2012.Alabau V. Rodríguez-Ruiz L. Sanchis A. Martínez-Gómez P. Casacuberta F. 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Distribution of melanopsin positive neurons in pigmented and albino mice: evidence for melanopsin interneurons in the mouse retina.

Here we have studied the population of intrinsically photosensitive retinal ganglion cells (ipRGCs) in adult pigmented and albino mice. Our data show that although pigmented (C57Bl/6) and albino (Swiss) mice have a similar total number of ipRGCs, their distribution is slightly different: while in pigmented mice ipRGCs are more abundant in the temporal retina, in albinos the ipRGCs are more abundant in superior retina. In both strains, ipRGCs are located in the retinal periphery, in the areas of lower Brn3a(+)RGC density. Both strains also contain displaced ipRGCs (d-ipRGCs) in the inner nuclear layer (INL) that account for 14% of total ipRGCs in pigmented mice and 5% in albinos. Tracing from both superior colliculli shows that 98% (pigmented) and 97% (albino) of the total ipRGCs, become retrogradely labeled, while double immunodetection of melanopsin and Brn3a confirms that few ipRGCs express this transcription factor in mice. Rather surprisingly, application of a retrograde tracer to the optic nerve (ON) labels all ipRGCs, except for a sub-population of the d-ipRGCs (14% in pigmented and 28% in albino, respectively) and melanopsin positive cells residing in the ciliary marginal zone (CMZ) of the retina. In the CMZ, between 20% (pigmented) and 24% (albino) of the melanopsin positive cells are unlabeled by the tracer and we suggest that this may be because they fail to send an axon into the ON. As such, this study provides the first evidence for a population of melanopsin interneurons in the mammalian retina

Powdery mildew resistance genes in vines: an opportunity to achieve a more sustainable viticulture

Grapevine (Vitis vinifera) is one of the main fruit crops worldwide. In 2020, the total surface area planted with vines was estimated at 7.3 million hectares. Diverse pathogens affect grapevine yield, fruit, and wine quality of which powdery mildew is the most important disease prior to harvest. Its causal agent is the biotrophic fungus Erysiphe necator, which generates a decrease in cluster weight, delays fruit ripening, and reduces photosynthetic and transpiration rates. In addition, powdery mildew induces metabolic reprogramming in its host, affecting primary metabolism. Most commercial grapevine cultivars are highly susceptible to powdery mildew; consequently, large quantities of fungicide are applied during the productive season. However, pesticides are associated with health problems, negative environmental impacts, and high costs for farmers. In paralleled, consumers are demanding more sustainable practices during food production. Therefore, new grapevine cultivars with genetic resistance to powdery mildew are needed for sustainable viticulture, while maintaining yield, fruit, and wine quality. Two main gene families confer resistance to powdery mildew in the Vitaceae, Run (Resistance to Uncinula necator) and Ren (Resistance to Erysiphe necator). This article reviews the powdery mildew resistance genes and loci and their use in grapevine breeding program

Histopathological Analogies in Chronic Pulmonary Lesions between Cattle and Humans: Basis for an Alternative Animal Model

Most of the natural cases of pneumonia in feedlot cattle are characterized by a longer clinical course due to chronic lung lesions. Microscopically, these lesions include interstitial fibroplasia, bronchitis, bronchiectasis, bronchiolitis obliterans, and epithelial metaplasia of the airways. Herein, the aim was to review, under a medical perspective, the pathologic mechanisms operating in these chronic pneumonic lesions in calves. Based on the similarities of these changes to those reported in bronchiolitis obliterans/organising pneumonia (BO/OP) and chronic obstructive pulmonary disease (COPD) in human beings, calves are proposed as an alternative animal model

The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species

Cortisol carries out its physiological mechanism of action through the recognition by the mineralocorticoid receptor (MR) and the glucocorticoid receptor (GR) 1 (GR1) and GR2. Previous studies reported that the main difference between gr1 and gr2 nucleotide sequences resides in a 27-nucleotide insertion/deletion in the DNA-binding region, respectively. However, in gilthead sea bream (Sparus aurata) the annotation for gr1 and gr2 seems contradictory. The gr2 sequence possesses the characteristic 27-nucleotide insertion that, in fact, is associated with the gr1 nucleotide sequence. Thus, this study aimed to elucidate the nucleotide sequences for the gr1 and gr2 in gilthead sea bream. The Clustal Omega alignment for different fish species corroborated the presence of such 27-nucleotide insertion/deletion in the DNA-binding region for gr1 and gr2, respectively. Then, we design specific primers set for the amplification of the gilthead sea bream gr1 by polymerase chain reaction (PCR). Importantly, the gr1 nucleotide partial sequence has a high similarity with other gr1 sequences already published for other fish species, being present in all of them the 27-nucleotide insertion in the DNA-binding region. We also detected that in European sea bass the gr1 and gr2 sequences had not been named according to the 27-nucleotide insertion/deletion criteria in the DNA-binding region. Thus, our study makes an urgent call to the scientific community to discuss the establishment of an updated agreement that allows homogenizing the criteria for the nomenclature defining the gr1 and gr2 nucleotide sequences for a better understanding of the stress response in teleost fish species.This study thanks to the AGL2016-76069-C2-2- R, PID2020-117557RB-C21, PID2020-117557RB-C22 grants (AEI-MINECO; Spain). EV-V thanks the support of Fondecyt iniciacion grant (project number 11221308; Agencia Nacional de Investigacion y Desarrollo de Chile, Government of Chile). AK was the recipient of a Ministry of Science, Research, and Technology (Iran) fellowship. MT thanks for the support of the post-doctoral fellowship "Ramon y Cajal" (ref. RYC2019-026841-I) (Ministerio de Ciencia e Innovacion, Spanish Government). FER-L thanks the support of Fondecyt regular grant (project number: 1211841; Agencia Nacional de Investigacion y Desarrollo de Chile, Government of Chile)

Searches for the Violation of Pauli Exclusion Principle at LNGS in VIP(-2) experiment

The VIP (Violation of Pauli exclusion principle) experiment and its follow-up experiment VIP-2 at the Laboratori Nazionali del Gran Sasso (LNGS) search for X-rays from Cu atomic states that are prohibited by the Pauli Exclusion Principle (PEP). The candidate events, if they exist, will originate from the transition of a $2p$ orbit electron to the ground state which is already occupied by two electrons. The present limit on the probability for PEP violation for electron is 4.7 $\times10^{-29}$ set by the VIP experiment. With upgraded detectors for high precision X-ray spectroscopy, the VIP-2 experiment will improve the sensitivity by two orders of magnitude.Comment: 5 pages, 3 figures, 1 table. Conference proceedings for oral presentation at TAUP 2015, Torin