Occurrence and genetic characterization of Listeria spp. in minimally processed vegetables commercialized in Porto Alegre, Brazil

Minimally processed vegetables go through many steps before they are refrigerated, selection, washing, peeling, cutting, disinfection and finally packaging. However, if no care is taken at the origin of the raw materials and in the processing stages, there is a chance of finding pathogenic bacteria, such as Listeria monocytogenes, which are able to grow at low temperatures. The aim of this research was to verify the occurrence of Listeria sp. in minimally processed vegetables sold in Porto Alegre, Brazil, and the genetic relationship among the isolates. Minimally processed salads were sampled monthly from local supermarkets and analyzed by inoculation on Listeria Enrichment Broth and subsequent seeding on two selective media, Palcam and Modified Oxford Agar. The typical colonies were identified to species level and their intergenic region 16S-23S rDNA were amplified in order to verify the genetic variability. Species of Listeria were found in 23 of the 52 processed salad samples analyzed and L. monocytogenes was found in seven. The presence of L. monocytogenes in the samples is a health concern, as these salads are eaten without further treatment by the consumer. The amplification of the intergenic region 16S-23S rDNA, showed a great genetic diversity among the isolates, with 43 different patterns, proving the usefulness of this technique in epidemiologic studies

[salmonella Sp And Listeria Monocytogenes In Fully Cooked Ham Commercialized In Supermarkets Of Fortaleza (ce, Brazil): Risk Factor For Public Health].

The objective of this work was to evaluate the occurrence of Salmonella sp. and Listeria spp. in fully cooked ham without fat cover, stored under refrigeration, commercialized in supermarkets of Fortaleza (CE, Brazil). Forty samples of cooked ham, proceeding from eight trademarks, collected in 26 establishments, were analyzed according to the APHA and HBP for Salmonella sp. and Listeria spp. detection, respectively. Thirty percent of the samples were contaminated with Salmonella sp. and 42.50%, 22.50% e 2.5% with L. monocytogenes, L. innocua e L. welshimeri, respectively. It was also observed imperfections in the application of good manipulation practices in the supermarkets freezer section. It was verify that 34.61%, 57.69% e 15.38% of the establishments presented some sample contaminated with Salmonella sp., L. monocytogenes and both microorganisms respectively, including the area inspected by the six Regionals Executive General Offices of Fortaleza. These data show the necessity of a quality control improvement in all parts of food systems as an essential factor in the prevention of foodborne diseases.16657-6

Salmonella sp and Listeria monocytogenes in fully cooked ham commercialized in supermarkets of Fortaleza (CE, Brazil): risk factor for public health

The objective of this work was to evaluate the occurrence of Salmonella sp. and Listeria spp. in fully cooked ham without fat cover, stored under refrigeration, commercialized in supermarkets of Fortaleza (CE, Brazil). Forty samples of cooked ham, proceeding from eight trademarks, collected in 26 establishments, were analyzed according to the APHA and HBP for Salmonella sp. and Listeria spp. detection, respectively. Thirty percent of the samples were contaminated with Salmonella sp. and 42.50%, 22.50% e 2.5% with L. monocytogenes, L. innocua e L. welshimeri, respectively. It was also observed imperfections in the application of good manipulation practices in the supermarkets freezer section. It was verify that 34.61%, 57.69% e 15.38% of the establishments presented some sample contaminated with Salmonella sp., L. monocytogenes and both microorganisms respectively, including the area inspected by the six Regionals Executive General Offices of Fortaleza. These data show the necessity of a quality control improvement in all parts of food systems as an essential factor in the prevention of foodborne diseases.O objetivo deste trabalho foi avaliar a ocorrência de Salmonella sp. e Listeria spp. em presunto suíno cozido sem capa de gordura, mantido sob temperatura de refrigeração, comercializado em supermercados de Fortaleza (CE). O material estudado compreendeu quarenta amostras, provenientes de oito marcas comerciais, coletadas em 26 estabelecimentos. A pesquisa de Salmonella sp. e Listeria spp. foi efetuada segundo APHA e HBP, respectivamente. Foi constatado que 30% das amostras estavam contaminadas por Salmonella sp. e 42,50%, 22,50% e 2,5% por L. monocytogenes, L. innocua e L. welshimeri, respectivamente. Foram observadas, ainda, falhas na aplicação das boas práticas de manipulação na área de frios dos supermercados. Verificou-se que 34,61%, 57,69% e 15,38% dos estabelecimentos apresentaram alguma amostra contaminada por Salmonella sp., L. monocytogenes e ambos os microrganismos, respectivamente, abrangendo a área de fiscalização das seis Secretarias Executivas Regionais (SER) do município de Fortaleza. Estes dados mostram a necessidade de um aperfeiçoamento quanto ao controle de qualidade em todo elo da cadeia alimentar como fator fundamental na prevenção de enfermidades transmitidas por alimentos (ETA).65766

Incidence and genetic characterization of Listeria sp. from minimally processed vegetables in Porto Alegre- RS

Hortaliças minimamente processadas passam por etapas como: lavagem, seleção, descasque, corte e sanificação e, depois de embaladas, são armazenadas sob refrigeração. No entanto, se não houver cuidado na procedência da matéria-prima, no processamento e na higienização, há o risco de se encontrar bactérias patogênicas, entre elas Listeria monocytogenes, a qual pode crescer à temperatura de refrigeração. A listeriose manifesta-se como um resfriado, podendo progredir para meningite, meningoencefalite, septicemia e aborto. Este trabalho teve por objetivo verificar a ocorrência de Listeria sp. Em hortaliças folhosas minimamente processadas comercializadas em Porto Alegre – RS e verificar a relação genética entre os isolados. Saladas folhosas minimamente processadas foram coletadas mensalmente em supermercados locais. Destas amostras foram transferidos 25g para Caldo de Enriquecimento de Listeria, seguido de semeadura em ágar PALCAM e Oxford Modificado. Foi realizado isolamento e identificação das espécies das colônias características. Dos isolados identificados foi feita a amplificação da região intergênica 16S-23S do rDNA para verificação da variabilidade genética. Listeria sp. foi encontrada em 23 das 52 amostras, sendo L. monocytogenes encontrada em 7 amostras. A presença de L. monocytogenes é preocupante, pois estes produtos são consumidos sem nenhum tratamento pelo consumidor. Através da amplificação da egião intergênica 16S-23S do r DNA de Listeria sp., observou-se grande diversidade entre os 164 isolados, gerando 44 padrões, e linhagens distintas nas diferentes origens das amostras.Processed vegetables go through many steps like washing, selection, peeling, cutting and disinfection, and after being packed they are kept under refrigeration. However, if there is no care about the raw material origin and at the processing and disinfection steps, there is a chance of finding pathogenic bacterias, like Listeria monocytogenes, which can grow at low temperatures. The symptoms of listeriosis are similar to a cold and they can progress to meningitis, meningoencephalitis, septicemia and abortion. The aim of the present work was to verify the incidence of Listeria sp. in ready to use processed vegetables and to analyze the genetic relationship among the isolates. Ready to use salads were sampled at local supermarkets every month and analyzed by inoculating them on Listeria Enrichment Broth and afterwards seeding on two selective media, Palcam and Modified Oxford Agar. The characteristics colonies were isolated and identified until species level. The intergenic region 16S-23S rDNA of all identified isolates was amplified in order to verify their genetic variability. Listeria sp. was found in 23 of the 52 samples of ready to use salads, L. monocytogenes was found in seven samples. The presence of L. monocytogenes is also a health concern, once these salads are consumed without a previous treatment. The amplification of the intergenic region showed a great genetic diversity among the 164 isolates, once it has generated 44 different patterns, and it has also proved to be useful in epidemiologic studies, once the same pattern could be observed at different salad and at different time

Occurrence and genetic characterization of Listeria spp. in minimally processed vegetables commercialized in Porto Alegre, Brazil

Minimally processed vegetables go through many steps before they are refrigerated, selection, washing, peeling, cutting, disinfection and finally packaging. However, if no care is taken at the origin of the raw materials and in the processing stages, there is a chance of finding pathogenic bacteria, such as Listeria monocytogenes, which are able to grow at low temperatures. The aim of this research was to verify the occurrence of Listeria sp. in minimally processed vegetables sold in Porto Alegre, Brazil, and the genetic relationship among the isolates. Minimally processed salads were sampled monthly from local supermarkets and analyzed by inoculation on Listeria Enrichment Broth and subsequent seeding on two selective media, Palcam and Modified Oxford Agar. The typical colonies were identified to species level and their intergenic region 16S-23S rDNA were amplified in order to verify the genetic variability. Species of Listeria were found in 23 of the 52 processed salad samples analyzed and L. monocytogenes was found in seven. The presence of L. monocytogenes in the samples is a health concern, as these salads are eaten without further treatment by the consumer. The amplification of the intergenic region 16S-23S rDNA, showed a great genetic diversity among the isolates, with 43 different patterns, proving the usefulness of this technique in epidemiologic studies

Microbiological quality of minimally processed vegetables sold in Porto Alegre, Brazil

Hortaliças minimamente processadas passam por várias etapas durante seu processamento, no qual ocorrem várias modificações de sua estrutura natural, todavia elas devem manter a mesma qualidade do produto não processado. O objetivo deste estudo foi quantificar microrganismos mesófilos e psicrótróficos, coliformes totais e fecais e verificar a presença de E. coli, parasitas e sujidades em hortaliças minimamente processadas prontas para consumo. Foram analisadas 56 amostras para mesófilos e psicrotróficos pelo método de contagem em placas, com média mensal 4,7x105 a 1,6x108 UFC/g e de 7,9x106 a 2,7x108 UFC/g, respectivamente. Os coliformes foram analisados pela técnica dos tubos múltiplos, onde coliformes totais variaram de <3 a ≥ 2,4x104 NMP/g e coliformes fecais, de <3 a 1,1x104 NMP/g, e E. coli foi observada em oito amostras. De 52 amostras, 8 (15,3%) apresentaram oocistos de Eimeria spp. Sujidades, como fragmentos de insetos e ácaros jovens foram encontrados. Contaminação de origem fecal foi verificada no presente estudo, sugerindo falhas nas etapas do processamento das hortaliças, ou que o solo ou a água de irrigação também poderiam ser fontes de disseminação destes microrganismos.Minimally processed vegetables go through various steps during their preparation, with many modifications to their natural structure. However, they must maintain the same quality as the fresh produce. The aim of the present study was to quantify mesophilic and psychrotrophic microorganisms and total and faecal coliforms, and to assess the presence of Escherichia coli, parasites, and dirt material in ready-to-eat minimally processed vegetables. Fifty-six samples of minimally processed vegetables were analysed for the presence of mesophilic and psychrotrophic microorganisms by the plate-count method. Monthly means ranged from 4.7x105 to 1.6x108 CFU/g and from 7.9x106 to 2.7x108 CFU/g, respectively for mesophilic and psychrotrophic microorganisms. Coliforms were analysed by the multiple-tube method; total coliforms ranged from <3 to ≥ 2.4x104 MPN/g and faecal coliforms from <3 to 1.1x104 MPN/g. Escherichia coli was detected in eight samples. Out of 52 samples, eight (15.3%) contained oocysts of Eimeria spp.. Dirt matter, such as insect body parts and young mites, was also found. Contamination of faecal origin was observed in these samples, suggesting that either the sanitisation of the product was unsuccessful, or soil or irrigation water could be the source of these microorganisms

El Pueblo : diario republicano de Valencia: El Pueblo : diario republicano de Valencia - Año XXXI Número 11395 - 1924 mayo 30 (30/05/1924)

28

Occurrence and genetic characterization of Listeria spp. in minimally processed vegetables commercialized in Porto Alegre, Brazil

Minimally processed vegetables go through many steps before they are refrigerated, selection, washing, peeling, cutting, disinfection and finally packaging. However, if no care is taken at the origin of the raw materials and in the processing stages, there is a chance of finding pathogenic bacteria, such as Listeria monocytogenes, which are able to grow at low temperatures. The aim of this research was to verify the occurrence of Listeria sp. in minimally processed vegetables sold in Porto Alegre, Brazil, and the genetic relationship among the isolates. Minimally processed salads were sampled monthly from local supermarkets and analyzed by inoculation on Listeria Enrichment Broth and subsequent seeding on two selective media, Palcam and Modified Oxford Agar. The typical colonies were identified to species level and their intergenic region 16S-23S rDNA were amplified in order to verify the genetic variability. Species of Listeria were found in 23 of the 52 processed salad samples analyzed and L. monocytogenes was found in seven. The presence of L. monocytogenes in the samples is a health concern, as these salads are eaten without further treatment by the consumer. The amplification of the intergenic region 16S-23S rDNA, showed a great genetic diversity among the isolates, with 43 different patterns, proving the usefulness of this technique in epidemiologic studies

Perfil de resistência antimicrobiana de Escherichia coli e Salmonella sp. isolados de hortaliças folhosas prontas para consumo comercializadas em Porto Alegre-RS

39