Principales desviaciones de la salud en recién nacidos sanos

Todo recién nacido debe afrontar un periodo de adaptación a la vida independiente tras el parto, que si no es capaz de superar, puede dar lugar a una desviación de su salud. Concretamente en este trabajo nos hemos decidido por una serie de alteraciones que ocurren de manera habitual durante la estancia hospitalaria, hecho que desde nuestra posición enfermera nos permite intervenir para favorecer la detección y resolución precoz. Las situaciones descritas son aquellas en las que se ven afectados los niveles séricos de bilirrubina o glucosa y la alteración en la eliminación y la ingesta, esta última asociada a un defecto en la succión. Para ello, se ha obtenido la información a partir de una búsqueda bibliográfica mediante diferentes bases de datos durante la cual el requisito indispensable ha sido que el niño naciera sin ningún tipo de patología asociada. Se describirán las intervenciones que puede llevar a cabo la enfermera para poder prevenir o solucionar estas entidades, no sólo destinadas a la actuación sobre el niño sino también a la ayuda hacia sus padres en el afrontamiento de esta etapa y en la proporción del conocimiento necesario para el posterior cuidado del niño tras el alta hospitalaria. Concluyendo con la utilidad de instaurar protocolos para que la enfermera pueda encaminar su intervención si detecta una posible manifestación de alguna de las alteraciones desarrolladas.Grado en Enfermerí

Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon

Brachypodium distachyon (2n = 2x = 10) is a small annual grass species where the existence of three different cytotypes (10, 20 and 30 chromosomes) has long been regarded as a case of autopolyploid series, with x = 5. However, it has been demonstrated that the cytotypes assumed to be polyploids represent two separate Brachypodium species recently named as B. stacei (2n = 2x = 20) and B. hybridum (2n = 4x = 30). The aim of this study was to find a PCR-based alternative approach that could replace standard cytotyping methods (i. e., chromosome counting and flow cytometry) to characterize each of the three Brachypodium species. We have analyzed with four microsatellite (SSR) markers eighty-three Brachypodium distachyon-type lines from varied locations in Spain, including the Balearic and Canary Islands. Within this set of lines, 64, 4 and 15 had 10, 20 and 30 chromosomes, respectively. The surveyed markers produced cytotype-specific SSR profiles. So, a single amplification product was generated in the diploid samples, with non-overlapping allelic ranges between the 2n = 10 and 2n = 20 cytotypes, whereas two bands, one in the size range of each of the diploid cytotypes, were amplified in the 2n = 30 lines. Furthermore, the remarkable size difference obtained with the SSR ALB165 allowed the identification of the Brachypodium species by simple agarose gel electrophoresis

Cines nacionales y lenguas no hegemónicas. La invisibilidad del gallego frente a las políticas de diversidad cultural

In the spirit of the proposals of the Agenda 2020 about the structural role of cinema in the configuration of the European identities, this article highlights the significance of the national cinemas in non-hegemonic languages in the conformation of a diverse European culture. Following this perspective, we use Galician cinema as a case study in which we analyze the presence (or more precisely the absence) of the Galician language in the original version in the feature films released between 2008 and 2012.This proposal is hosted by the I+D+I project eDCINEMA: “Towards the European Digital Space. The role of small cinemas in original version” (Ref. CSO2012-35784) financed by the Ministry of Economy and Competitiveness of Spain.En línea con los postulados de la Agenda 2020 respecto al rol estructural del cine en la configuración de la(s) identidad(es) europea(s), el artículo destaca la trascendencia de las cinematografías nacionales en lenguas no hegemónicas en la consecución de una cultura europea diversa. Desde esta perspectiva, se asume el estudio de caso del cine en Galicia, y se analiza la presencia (o, para ser más exactos, la ausencia) de la versión original gallega en los largometrajes cinematográficos estrenados entre 2008 y 2012. La propuesta surge al abrigo del proyecto de I+D+i “Hacia el Espacio Digital Europeo. El papel de las cinematografías pequeñas en versión original” (Ministerio de Economía y Competitividad. Ref. CSO2012- 35784)

Arquitectura como disposición: Una aproximación al proyecto de arquitectura en la trastienda europea del cambio de siglo

No muchas palabras contienen una aptitud y actitud, un modo de hacer y las implicaciones que de ello se derivan. Disposición es tanto la acción como el efecto de disponer. Ante la encrucijada que con anterioridad plantearan R. Venturi (1966) y M. Tafuri (1977) para la arquitectura, tras el colapso del proyecto moderno y re-enunciación crítica de sus preceptos - Jenks (1978), Habermas (1980), Jameson (1983)-, la arquitectura como disposición plantea una lectura crítica y diferencial de la disciplina y propone la configuración de ésta como práctica que aúna teoría y proyecto. Este modo de plantear la profesión conlleva asumir también, entre otras cuestiones, la tarea de redefinir el contrato natural (Serres, 1990) o el encargo (Sloterdijk, 1999) en que se fundamentaba. Mientras la arquitectura se resitúa transversalmente junto a otras disciplinas - ecología política, antropología, prácticas culturales y técnicas ambientales - esta investigación estudia el proyecto de arquitectura como herramienta de proyección contemporánea de una idea de lo urbano, lo cultural y de una idea de sujeto, que han sido escasamente tipificadas en este periodo. Recorre prácticas artísticas y arquitectónicas donde reconocer dichas herramientas de proyección, propone una actualización y reflexión de los conceptos de de recinto, pauta y materia, que se despliegan como de lógicas de proyecto no prescriptivas sino dispuestas. Pensar arquitectura como disposición permite entenderla participando de los dos extremos: como enunciado o modo de estar con sentido en una época, y como traslación de este sentido al proyecto y la obra de arquitectura

Modelling the performance of a SiC-based synchronous boost converter using different conduction modes

IEEE Applied Power Electronics Conference and Exposition (APEC) (33th, 2018, San Antonio, Texas

Impact of different levels of handling on Solea senegalensis culture: effects on growth and molecular markers of stress

[EN] Aquaculture routine practices may cause stress induction on the fish and compromise their welfare affecting the production. This experiment aimed to evaluate the potential links between handling during culture with stress responses and growth on Senegalese sole (Solea senegalensis). We worked with two fish cohorts in terms of initial body weight and culture stage: Trial 1 included specimens in the fattening stage (226 ± 4.96 g) and Trial 2 animals in the pre-fattening stage (27.20 ± 0.44 g). The tested culture protocol, which lasted 6 and 4 months for Trial 1 and 2, respectively, mainly reduced handling-derived stressors in the experimental tanks via lowering routine samplings to a minimum. This decrease of the handling-derived stress was reflected in both trials with lower concentration of circulating cortisol in blood plasma from the experimental fish when compared to controls. Moreover, the proposed protocol promoted higher growth in the fish cultured in the less disturbing protocol in Trial 2. Higher specific growth rates and mean body weight and length were reported. In order to further explore the potential beneficial effects of our protocol, we studied the musculoskeletal from Trial 2 gene expression of key genes regulating glucocorticoid signaling pathway and apoptosis: glucocorticoid receptors 1 and 2 (gr1, gr2), heat shock protein 90 AA (hsp90aa), and caspase 6 (casp6). In line with the cortisol reduced level in this trial, gr1, hsp90aa, and casp6 genes showed lower expression in the samples coming from the experimental group. The findings of this study provide valuable information to the aquaculture industry for the management of Solea senegalensis stress and welfare.SIPublicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCL

Probiotics reduce anxiety-related behavior in zebrafish

[EN] There is increasing evidence that gut microbiome could have effects on neurological processes and on behavior. In this study we used the novel tank test (NTT) to analyze zebrafish exploring behavior after four months’ supplementation with probiotics with probed antioxidant and anti-inflammatory properties. Results showed that prolonged ingestion of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347 significantly alters the swimming pattern and mean swimming speed in the zebrafish model. After treatment, zebrafish strongly reduced their bottom-dwelling geotactic behavior when placed in a new tank, which could be correlated to a lower state of anxiety.S

Effect of low sperm quality on progeny: a study on zebrafish as model species

[EN] Nowadays a decrease tendency in human sperm quality has been reported mainly in developed countries. Reproductive technologies have been very valuable in achieving successful pregnancies with low quality sperm samples. However, considering that spermatozoa molecular contribution is increasingly important in recent studies, it is crucial to study whether fertilization with low sperm quality could leave a molecular mark on progeny. This study explores the consequences that fertilization with low sperm quality may have on progeny, using zebrafish as a model. Good and bad breeders were established attending to sperm quality analyses and were individually tracked. Significant differences in fertilization and malformation rates were obtained in progenies between high and low quality sperm samples. Moreover an altered miR profile was found in the progenies of bad zebrafish breeders (upregulation of miR-141 and miR -122 in 24 hpf embryos) and as a consequence, some of their targets involved in male sex development such as dmrt1, suffered downregulation. Our results indicate that fertilizing with high sperm quality samples becomes relevant from a new perspective: to avoid molecular alterations in the progeny that could remain masked and therefore produce unexpected consequences in it.SIThis work was supported by AGL2015 68330-C2-1-R project (MINECO/FEDER), PTA2016-11987-I contract (MINECO/FEDER) and AQUA-CIBUS international net 318RT0549 funded by CYTED (Programa Iberoamericano de Ciencia y Tecnologia para el Desarrollo

Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality after Only One Spermatogenic Cycle in Zebrafish Model

[EN] Infertility is a medical concern worldwide and could also have economic consequences in farmed animals. Developing an efficient diet supplement with immediate effects on sperm quality is a promising tool for human reproduction and for domesticated animal species. This study aims at elucidating the effect of a short-time probiotic supplementation consisting of a mixture of two probiotic bacteria with proven antioxidant and anti-inflammatory activities on zebrafish sperm quality and male behavior. For this purpose, three homogeneous groups of males in terms of motility (<60%) were established. The control group was fed with a normal standard diet. The other received supplements: One group (vehicle control) was fed with maltodextrin and the other received a probiotic preparation based on a mixture (1:1) of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347. The feeding regime was 21 days corresponding with a single spermatogenesis in zebrafish. The preparation did not modify animal weight, positively affected the number of fluent males, increased sperm concentration, total motility, progressive motility, and fast spermatozoa subpopulations. Moreover, the animals fed with the supplement showed different behavior patterns compared to control groups. Our results suggest a diet-related modulation on the exploration activity indicating a lower stress-like conduct. The studied formulation described here should be considered as advantageous in male reproductive biotechnologySIThis work was supported by project AGL2015 68330-C2-1-R (MINECO/FEDER)The authors acknowledge contract PTA2016-11987-I (MINECO/FEDER), Biopolis S.L. (Valencia, Spain), AQUA-CIBUS International Net 318RT0549 (CYTED), and the staff from Planta de Cultivos El Bocal (IEO