A microbiological profile of commercially prepared salads

Samples of commercially prepared chicken salad, ham salad, potato salad, cole slaw, pimento cheese spread and several other types of salads from producers were analyzed microbiologically upon receipt and five days after the expiration date for aerobic plate count, psychrophilic plate count, coliform count and yeast and mold count. Plating was carried out by homogenizing ten grams of each salad with ten milliliters of 2 percent sodium citrate to a pipettable consistency in a Virtis homogenizer and pouring plates. Aerobic plate count ranges of less than 100 to greater than 30 million organisms per gram of fresh salad and from less than 100 to greater than 1.5 billion organisms per gram of salad after the expiration date were found. The psychrophilic plate count range was from less than 100 to 31 million organisms per gram of fresh salad and from less than 100 to greater than 970 million organisms per gram of salad after the expiration date. Coliform plate count ranges were from less than one to 1,990 coliforms per gram of salad after the expiration date. Yeast and mold plate count ranges were from less than one to greater than 300,000 organisms per gram of fresh salad and from less than one to greater than 300,000 organisms per gram of salad after the expiration date.. Bacteria from aerobic plate counts, psychrophilic plate counts and coliform counts were isolated according to differences in colony type and location in the agar. The bacteria were characterized for identification. Identified bacteria included eight species of Bacillus, six species of Lactobacillus, three species of Leuconostoc, five species of Streptococcus, three species of Micrococcus, one species of Escherichia, one species of Enterobacter and one species of Citrobacter. Members of the genera of Pseudomonas, Xanthomonas and Actinomyces were also found

Pronounced inter-individual variation in plasma cortisol response to fluoxetine hydrochloride in the pig

Published: 18 March 2020Animal welfare assessment requires measures for positive affective state. Pharmacological agents that manipulate affective state can be used to evaluate novel biomarkers for affective state assessment. However, to validate that an agent has modified brain function, a reliable indicator is required. Circulating cortisol has been used as a reporter for effective delivery of the antidepressant selective serotonin reuptake inhibitor (SSRI) fluoxetine hydrochloride to the brain in humans and sheep. Here, we tested cortisol as a reporter for effective delivery of fluoxetine hydrochloride to the pig brain. We hypothesized that following administration of SSRI, innervation of the serotonergic reward pathway would result in activation of the hypothalamic-pituitary-adrenal (HPA) axis, leading to increased circulating cortisol levels. Large White-Landrace gilts received either a single intravenous dose of 100 mg fluoxetine hydrochloride suspended in 10 mL saline (n = 4), or 10 mL saline alone (n = 4). Blood samples were collected every 15 min for one hour prior to, and six hours post-treatment. The interaction of treatment x time on mean plasma cortisol levels between 15–165 min post-treatment was significant (p = 0.048) with highest cortisol concentrations of SSRI treated pigs versus controls (+ 98%) at 135 min post-treatment. However, individual cortisol profiles after SSRI treatment revealed high inter-individual variation in response. We conclude that, while combined data imply that plasma cortisol may be a readout for SSRI efficacy, inter-individual variation in SSRI response may preclude application of this approach in the pig. Given the current limited sample size, further research to confirm these findings is needed.Laura E. Marsh, Robyn Terry, Alexandra L. Whittaker, Stefan Hiendleder and Cameron R. Ralp

REDUCE-IT USA: Results From the 3146 Patients Randomized in the United States.

BackgroundSome trials have found that patients from the United States derive less benefit than patients enrolled outside the United States. This prespecified REDUCE-IT (Reduction of Cardiovascular Events with Icosapent Ethyl - Intervention Trial) subgroup analysis was conducted to determine the degree of benefit of icosapent ethyl in the United States.MethodsREDUCE-IT randomized 8179 statin-treated patients with qualifying triglycerides ≥135 and <500 mg/dL and low-density lipoprotein cholesterol >40 and ≤100 mg/dL and a history of atherosclerosis or diabetes mellitus to icosapent ethyl 4 g/d or placebo. The primary composite end point was cardiovascular death, nonfatal myocardial infarction, nonfatal stroke, coronary revascularization, or hospitalization for unstable angina. The key secondary composite end point was cardiovascular death, nonfatal myocardial infarction, or nonfatal stroke. A hierarchy was prespecified for examination of individual and composite end points.ResultsA total of 3146 US patients (38.5% of the trial) were randomized and followed for a median of 4.9 years; 32.3% were women and 9.7% were Hispanic. The primary composite end point occurred in 24.7% of placebo-treated patients versus 18.2% of icosapent ethyl-treated patients (hazard ratio [HR], 0.69 [95% CI, 0.59-0.80]; P=0.000001); the key secondary composite end point occurred in 16.6% versus 12.1% (HR, 0.69 [95% CI, 0.57-0.83]; P=0.00008). All prespecified hierarchical end points were meaningfully and significantly reduced, including cardiovascular death (6.7% to 4.7%; HR, 0.66 [95% CI, 0.49-0.90]; P=0.007), myocardial infarction (8.8% to 6.7%; HR, 0.72 [95% CI, 0.56-0.93]; P=0.01), stroke (4.1% to 2.6%; HR, 0.63 [95% CI, 0.43-0.93]; P=0.02), and all-cause mortality (9.8% to 7.2%; HR, 0.70 [95% CI, 0.55-0.90]; P=0.004); for all-cause mortality in the US versus non-US patients, Pinteraction=0.02. Safety and tolerability findings were consistent with the full study cohort.ConclusionsWhereas the non-US subgroup showed significant reductions in the primary and key secondary end points, the US subgroup demonstrated particularly robust risk reductions across a variety of individual and composite end points, including all-cause mortality.Clinical trial registrationURL: https://www.clinicaltrials.gov. Unique identifier: NCT01492361

Monocyte Subset Dynamics in Human Atherosclerosis Can Be Profiled with Magnetic Nano-Sensors

Monocytes are circulating macrophage and dendritic cell precursors that populate healthy and diseased tissue. In humans, monocytes consist of at least two subsets whose proportions in the blood fluctuate in response to coronary artery disease, sepsis, and viral infection. Animal studies have shown that specific shifts in the monocyte subset repertoire either exacerbate or attenuate disease, suggesting a role for monocyte subsets as biomarkers and therapeutic targets. Assays are therefore needed that can selectively and rapidly enumerate monocytes and their subsets. This study shows that two major human monocyte subsets express similar levels of the receptor for macrophage colony stimulating factor (MCSFR) but differ in their phagocytic capacity. We exploit these properties and custom-engineer magnetic nanoparticles for ex vivo sensing of monocytes and their subsets. We present a two-dimensional enumerative mathematical model that simultaneously reports number and proportion of monocyte subsets in a small volume of human blood. Using a recently described diagnostic magnetic resonance (DMR) chip with 1 µl sample size and high throughput capabilities, we then show that application of the model accurately quantifies subset fluctuations that occur in patients with atherosclerosis

The Subsystems Approach to Genome Annotation and its Use in the Project to Annotate 1000 Genomes

The release of the 1000(th) complete microbial genome will occur in the next two to three years. In anticipation of this milestone, the Fellowship for Interpretation of Genomes (FIG) launched the Project to Annotate 1000 Genomes. The project is built around the principle that the key to improved accuracy in high-throughput annotation technology is to have experts annotate single subsystems over the complete collection of genomes, rather than having an annotation expert attempt to annotate all of the genes in a single genome. Using the subsystems approach, all of the genes implementing the subsystem are analyzed by an expert in that subsystem. An annotation environment was created where populated subsystems are curated and projected to new genomes. A portable notion of a populated subsystem was defined, and tools developed for exchanging and curating these objects. Tools were also developed to resolve conflicts between populated subsystems. The SEED is the first annotation environment that supports this model of annotation. Here, we describe the subsystem approach, and offer the first release of our growing library of populated subsystems. The initial release of data includes 180 177 distinct proteins with 2133 distinct functional roles. This data comes from 173 subsystems and 383 different organisms

SN 2008gz - most likely a normal type IIP event

We present BV RI photometric and low-resolution spectroscopic investigation of a type II core-collapse supernova (SN) 2008gz, which occurred in a star forming arm and within a half-light radius (solar metallicity region) of a nearby spiral galaxy NGC 3672. The SN event was detected late and a detailed investigation of its light curves and spectra spanning 200 days suggest that it is an event of type IIP similar to archetypal SNe 2004et and 1999em. However, in contrast to other events of its class, the SN 2008gz exhibits rarely observed V magnitude drop of 1.5 over the period of a month during plateau to nebular phase. Using 0.21 mag of Av as a lower limit and a distance of 25.5 Mpc, we estimate synthesized $^{56}$Ni mass of 0.05 \pm 0.01 M* and a mid-plateau Mv of -16.6 \pm 0.2 mag. The photospheric velocity is observed to be higher than that was observed for SN 2004et at similar epochs, indicating explosion energy was comparable to or higher than SN 2004et. Similar trend was also seen for the expansion velocity of H-envelopes. By comparing its properties with other well studied events as well as by using a recent simulation of pre-SN models of Dessart, Livne & Waldman (2010), we infer an explosion energy range of 2 - 3 x 10$^{51}$ erg and this coupled with the observed width of the forbidden [O I] 6300-6364 {\AA} line at 275 days after the explosion gives an upper limit for the main-sequence (non-rotating, solar metallicity) progenitor mass of 17 M*. Our narrow-band H{\alpha} observation, taken nearly 560 days after the explosion and the presence of an emission kink at zero velocity in the Doppler corrected spectra of SN indicate that the event took place in a low luminosity star forming H II region.Comment: Accepted for publication in MNRA

Re-Shuffling of Species with Climate Disruption: A No-Analog Future for California Birds?

By facilitating independent shifts in species' distributions, climate disruption may result in the rapid development of novel species assemblages that challenge the capacity of species to co-exist and adapt. We used a multivariate approach borrowed from paleoecology to quantify the potential change in California terrestrial breeding bird communities based on current and future species-distribution models for 60 focal species. Projections of future no-analog communities based on two climate models and two species-distribution-model algorithms indicate that by 2070 over half of California could be occupied by novel assemblages of bird species, implying the potential for dramatic community reshuffling and altered patterns of species interactions. The expected percentage of no-analog bird communities was dependent on the community scale examined, but consistent geographic patterns indicated several locations that are particularly likely to host novel bird communities in the future. These no-analog areas did not always coincide with areas of greatest projected species turnover. Efforts to conserve and manage biodiversity could be substantially improved by considering not just future changes in the distribution of individual species, but including the potential for unprecedented changes in community composition and unanticipated consequences of novel species assemblages