Preliminary investigations on an enzyme immobilized optical biosensor for arsenite detection

Arsenite is an inorganic form of arsenic that poses hazardous effect to human. It is a common environmental heavy metal contaminant ubiquitously found in water and groundwater. In this study, an optical biosensor for arsenite determination was developed by immobilization of crude arsenite oxidase (Aio) extracted from recombinant E. coli, in chitosan solution coated on triacetyl-cellulose membrane employing DCPIP as colour indicator. The arsenite oxidase (Aio) was successfully expressed and extracted from recombinant E. coli strain BL21 (DE3). The protein concentration and specific activity of the crude arsenite oxidase were determined. Expression of Aio was confirmed by SDS-PAGE. The crude Aio was also successfully immobilized in chitosan and coated on triacetyl cellulose membrane. The response time and dynamic range of the optical biosensor were optimized. The response time of the developed biosensor was 15 minutes. The amount of DCPIP reduced (ΔA) was inversely proportional to the arsenite concentration. Standard calibration curve for arsenite detection was achieved within the range of arsenite concentration from 25 μM to 200 μM. The maximum detection limit was determined to be 250 μM arsenite

Beyond ‘greeting’ and ‘thanking’: politeness in job interviews

Despite the steps taken by Malaysian institutions of higher learning to equip their graduates with the necessary communication skills, local graduates are still failing to create a positive impression on the employers especially during job interviews. Hence this study explored the face-related concept of politeness proposed by Brown and Levinson (1999) at job interviews. Eight final-year undergraduates taking a communication for employment course volunteered for a mock-interview session with a human resource manager from the banking industry. The interviews were audio- and video-recorded and subsequently transcribed. Then a micro-analysis of the data was carried out using Brown and Levinson’s (1999) framework to identify the politeness strategies that the candidates used. Two positive politeness strategies were identified, the first is noticing and attending to interviewer’s interests, wants, needs or goods, and second is raising/asserting common grounds to establish solidarity. One negative politeness strategy was identified which is requesting for clarification or repetition. The pedagogical implications of these findings will be discussed and recommendations for teaching face-related politeness in communication for employment courses will be offered

E-readiness to G-readiness: developing a green information technology readiness framework

Businesses are under increasing pressure from competitors, regulators and community groups to implement sustainable business practices. Balancing economic and environmental performance to be green and competitive is therefore a key strategic issue. The increased discussion on green information technology (IT) has sparked the interest of this research. Green IT is poised to influence not only technology but also competitive strategy and even the legality of some business strategic options. Understanding and leveraging Green IT is therefore critical for businesses¿ continued progress. Nevertheless, the principles, practices and value of Green IT is yet to be researched. This paper introduces the concept of Green IT and describes the main pillars of a g-readiness framework to help organisations evaluate their readiness for adopting Green IT. It argues that just as e-readiness has been, and continues to be, a critical quality in the digital economy, g-readiness is an equally critical quality in the low carbon digital economy. Without a clear understanding of g-readiness, organisations would approach Green IT initiatives on an ad hoc and somewhat reactive basis which is undesirable

Evolution of precision agriculture computing towards sustainable oil palm industry

Precision technology elements have not been implemented yet into the sustainable oil palm industry because the knowledge and technology gap. To resolve the gaps, promote sustainability and integrate the technologies, Oil Palm Management System (OPAMS) was introduced. The precision technologies in OPAMS comprises of Geographical Information System (GIS), Global Positioning System (GPS), remote sensing and yield monitoring. A phase by phase System Development Life Cycle (SDLC) methodology was used to generate the said system with feedbacks from oil palm planters as the inputs for OPAMS’s key features. OPAMS ultimately aims to increase the awareness of the industry on the benefits of utilizing technology to improve plantation performances, increase business and environmental sustainability

Economic evaluation of the introduction of the Prostate Health Index as a rule-out test to avoid unnecessary biopsies in men with prostate specific antigen levels of 4-10 in Hong Kong

A recent study showed that the Prostate Health Index may avoid unnecessary biopsies in men with prostate specific antigen 4-10ng/ml and normal digital rectal examination in the diagnosis of prostate cancer in Hong Kong. This study aimed to conduct an economic evaluation of the impact of adopting this commercially-available test in the Hong Kong public health service to determine whether further research is justified. A cost-consequence analysis was undertaken comparing the current diagnostic pathway with a proposed diagnostic pathway using the Prostate Health Index. Data for the model was taken from a prospective cohort study recruited at a single-institution and micro-costing studies. Using a cut off PHI score of 35 to avoid biopsy would cost HK$3,000 and save HK$7,988 per patient in biopsy costs and HK$511 from a reduction in biopsy-related adverse events. The net cost impact of the change was estimated to be HK$5,500 under base case assumptions. At the base case sensitivity and specificity for all grades of cancer (61.3% and 77.5% respectively) all grade cancer could be missed in 4.22% of the population and high grade cancer in 0.53%. The introduction of the prostate health index into the diagnostic pathway for prostate cancer in Hong Kong has the potential to reduce biopsies, biopsy costs and biopsy-related adverse events. Policy makers should consider the clinical and economic impact of this proposal

Tapered microchannel for multi-particles passive separation based on hydrodynamic resistance

Researches on separation of multi-particles utilizing microfluidic have been flourishing in recent years with the aid from advancements in microfabrication design and technology. Generally, separation is beneficial for biomedical application especially involving heterogeneous samples. Due to inherent problems of samples isolation, a simple and efficient separation device is required. Here, we present a passive tapered microchannel for multi-particles separation using hydrodynamic principle. Our emphasis is on the effect of hydrodynamic resistance coupled with tapered microchannel design. In the experiment, successful multi-particles samples separation was observed. The results were further analyzed and were in agreement with the proposed concept. This method opens the route toward robust, low-cost and high-throughput, thus it may holds potential to be integrated as one functional module in Micro Total Analysis System (µTAS)

Outpatient-Based Therapy of Oral Fludarabine and Subcutaneous Alemtuzumab for Asian Patients with Relapsed/Refractory Chronic Lymphocytic Leukemia

Background. Intravenous alemtuzumab and fludarabine are effective in combination for the treatment of chronic lymphocytic leukemia (CLL), but require hospital visits for intravenous injection. We performed a pilot study to assess the safety and efficacy of outpatient-based oral fludarabine with subcutaneous alemtuzumab (OFSA) for the treatment of relapsed/refractory CLL. Results. Depending on their response, patients were given two to six 28-day cycles of subcutaneous alemtuzumab 30 mg on days 1,3, and 5 and oral fludarabine 40 mg/m2/day for 5 days. Median patient age was 74. The lymphocyte counts of all five patients fell after the 1st cycle of treatment and reached normal/low levels on completion of 2 to 6 cycles of therapy. Platelet counts and hemoglobin were unaffected. All five patients achieved complete hematological remission, while two attained minimal residual disease negativity on 4-color flow cytometry. Conclusions. Our OFSA regimen was effective in elderly Asian patients with relapsed/refractory CLL, and it should be investigated further

Predictors of allergen sensitization in Singapore children from birth to 3 years

10.1186/s13223-016-0161-xAllergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology121Article number 56GUSTO (Growing up towards Healthy Outcomes

Visual-Based Anomaly Detection for BGP Origin AS Change (OASC) Events

To complement machine intelligence in anomaly event analysis and correlation, in this paper, we investigate the possibility of a human-interactive visual-based anomaly detection system for faults and security attacks related to the BGP (Border Gateway Protocol) routing protocol. In particular, we have built and tested a program, based on fairly simple information visualization techniques, to navigate interactively real-life BGP OASC (Origin AS Change) events. Our initial experience demonstrates that the integration of mechanical analysis and human intelligence can effectively improve the performance of anomaly detection and alert correlation. Furthermore, while a traditional representation of OASC events provides either little or no valuable information, our program can accurately identify, correlate previously unknown BGP/OASC problems, and provide network operators with a valuable high-level abstraction about the dynamics of BGP