Helicobacter pylori infection of gastric cancercells elevates the level of expression and activation of protein kinase D2

Aim: To test the hypothesis, whether H. pylori infection may affect the level of PKD2 expression and/or activation in gastric cancer cells. Methods: Studies were performed on AGS human gastric adenocarcinoma cell line, gastric tissues samples from 36 cases of different histological variants of gastric cancer. Immunohistochemical, cell and molecular biology, bacteriological and biochemical approaches have been used in this study. Results: H. pylori 16S rRNA gene was detected in 97% cases of gastric tumors, and in 83% of cases cаgA gene was detected. In all tested adenocarcinoma samples cagA+ H. pylori was revealed. These cases were characterized by high level of PKD1/2 expression and autophosphorylation. In adenogenic cancer samples the presence of cagA– H. pylori was identified. Carcinoid and nondifferentiated gastric cancers contain H. pylori, with very low numbers of cagA+ copies. All cases of gastric tumors with cagA– H. pylori had very low levels of PKD1/2 autophosphorylation. AGS cell line infection with cagA– and cagA+ H. рylori resulted in elevation of PKD2 expression levels in 3.29 and 3.66 times respectively (p < 0.001). In cells infected by cag+ H. рylori the level of PKD2 transphosphorylation was 1.39 higher than in cells infected by cagA– H. pylori. For PKD2 autophosphorylation this difference was even higher — 3.27 times (p < 0.001). Conclusion: H. pylori infection enhanced the level of protein kinase D2 expression, trans- and autophosphorylation. The level of PKD2 autophosphorylation/activation was higher in AGS cell line inoculated of with cag+ H. pylori than in AGS cells with cagA– H. pylori. These suggest that H. pylori induces activation of PKD1/2 and could exploit PKD2 mediated signaling pathways that may contribute to the pathogenesis of gastric cancer

Content of cobalt in biological fluidsoffull-term newborns as the predictor of perinatal hypoxic damage of CNS

Influence of Co content on the system mother-placenta-fetus-newborn children born with hypoxia. Determination of microelement (ME) performed in serum and erythrocytes 30 pregnant women and their infants who have suffered asphyxia at birth. Comparison group consisted of 30 healthy women and their 30 healthy full-term infants

Квантова трансформація шкільної інформатики

The study’s objective was to theoretically examine, create, and experimentally test various approaches for instructing lyceum students in the fundamentals of quantum informatics. The following was accomplished as a result of the research assignments: 1) The sources on the issue of teaching quantum informatics in Ukraine and abroad were examined; 2) The structure and content of the competences in the basics of quantum informatics for the lyceum students were theoretically grounded and developed (the results of the expert survey and the European competence framework in the field of quantum technologies were taken into consideration); 3) the structural and functional model for forming the competences in the basics of quantum informatics were developed; 4) in the optional course of the same name, it was proposed a methodological framework for teaching the fundamentals of quantum informatics to lyceum students; and 5) it was also experimentally tested to see how well the developed methodo logy worked in developing the students’ competency in the fundamentals of quantum informatics. Further scientific investigations into the quantum transformation of the school’s informatics are also described.Метою дослідження було теоретичне обґрунтування, створення та експериментальна перевірка різних підходів до навчання ліцеїстів основам квантової інформатики. У результаті виконання дослідницьких завдань було досягнуто наступне: 1) вивчено джерела з проблеми навчання квантової інформатики в Україні та за кордоном; 2) теоретично обґрунтовано та розроблено структуру та зміст компетентностей з основ квантової інформатики для ліцеїстів (враховано результати експертного опитування та Європейську рамку компетентностей у галузі квантових технологій) 3) розроблено структурно-функціональну модель формування компетентностей з основ квантової інформатики; 4) запропоновано методичні засади навчання основ квантової інформатики ліцеїстів у рамках однойменного факультативного курсу; 5) експериментально перевірено ефективність розробленої методики формування компетентностей з основ квантової інформатики в учнів ліцею. Також описано подальші наукові дослідження в галузі квантової трансформації шкільної інформатики

ТЕХНОЛОГИИ ПОЛУЧЕНИЯ НАНОСТРУКТУРИРОВАННЫХ ПОВЕРХНОСТЕЙ

This article discusses the application and technology for obtaining nano-structured surfaces. Methods such as compaction of powders (isostatic pressing, Gleiter method), severe plastic deformation (angular torsion, equal-channel angular pressing, pressure treatment of multilayer composites) and surface modification (laser treatment, ion bombardment) are considered.В данной статье рассматривается применение и технологии получения наноструктурированных поверхностей. Рассмотрены такие методы как компактирование порошков (изостатическое прессование, метод Гляйтера), интенсивная пластическая деформация (угловое кручение, равноканальное угловое прессование, обработка давлением многослойных композитов) и модификация поверхности (лазерная обработка, ионная бомбардировка)

Crystal structure of Rb2[GeO2(OH)2] •2H2O

Single crystals of Rb2[GeO2(OH)2] • 2H2O are studied by X-ray diffraction. The crystals belong to the orthorhombic system, sp. gr. Pna21, a = 13.523(6) Å, b = 8.143(4) Å, c = 13.407(6) Å, Z = 8, R 1 = 0.0506. In [GeO2(OH)2]2- anions, the Ge-O distances (1.71-1.73(1) Å) are shorter than the Ge-OH distances (1.76-1.80(1) Å). Anions are linked to each other by pairs of hydrogen bonds to form infinite chains. The chains are linked by hydrogen bonds involving water molecules to form a 3D structure. The assignment of the bands in the IR spectrum of the compound under study is performed. © Nauka/Interperiodica 2006

Crystal structure of Rb2[GeO2(OH)2] •2H2O

Single crystals of Rb2[GeO2(OH)2] • 2H2O are studied by X-ray diffraction. The crystals belong to the orthorhombic system, sp. gr. Pna21, a = 13.523(6) Å, b = 8.143(4) Å, c = 13.407(6) Å, Z = 8, R 1 = 0.0506. In [GeO2(OH)2]2- anions, the Ge-O distances (1.71-1.73(1) Å) are shorter than the Ge-OH distances (1.76-1.80(1) Å). Anions are linked to each other by pairs of hydrogen bonds to form infinite chains. The chains are linked by hydrogen bonds involving water molecules to form a 3D structure. The assignment of the bands in the IR spectrum of the compound under study is performed. © Nauka/Interperiodica 2006