DEVELOPMENT OF ENTREPRENEURIAL ACTIVITIES â€" PREMISE OF THE INCREASE OF ECONOMIC COMPETITIVENESS

In the context of the integration into the European Union, the issue of the increase of firmsâ€(tm) competitiveness has become essential for Romaniaâ€(tm)s economy also, which requires as a mandatory condition the elaboration and application of certain clear strategies, both at the level of firms, and of the Government's, with specific goals and actions. Although it has made certain progress, Romania has significant competitiveness delays compared to the EU member states. In this context, development of entrepreneurial activities is a premise of the increase of economic competitiveness. Entrepreneurship is first of all a state of mind referring to the motivation and capacity of an individual to identify opportunity and follow it with the purpose of producing value or economic success.entrepreneurship, competitiveness, development

GENDER DISPARITIES IN THE ENTREPRENEURIAL FIELD IN WESTERN ROMANIA

The entrepreneurial spirit may develop in any economic sector and any type of business, both in the case of men and women, it is applied to a wide range of organisations, from family businesses to large companies, irrespective of the structure of shareholders, from NGOs to companies gone public on the capital market, firms from all sectors of activity. As regards the gender disparities in the entrepreneurial field, studies reveal the fact that although the two genders are similar, women entrepreneurs differ from men through motivations, their business ability and occupational past. Although the gender differences are still important, the number of women entrepreneurs has significantly increased compared to the previous period.entrepreneurship, gender disparities, motivations

Sculpting DNA-based synthetic cells through phase separation and phase-targeted activity

Synthetic cells, like their biological counterparts, require internal compartments with distinct chemical and physical properties where different functionalities can be localized. Inspired by membrane-less compartmentalization in biological cells, here, we demonstrate how microphase separation can be used to engineer heterogeneous cell-like architectures with programmable morphology and compartment-targeted activity. The synthetic cells self-assemble from amphiphilic DNA nanostructures, producing core-shell condensates due to size-induced de-mixing. Lipid deposition and phase-selective etching are then used to generate a porous pseudo-membrane, a cytoplasm analog, and membrane-less organelles. The synthetic cells can sustain RNA synthesis via in vitro transcription, leading to cytoplasm and pseudo-membrane expansion caused by an accumulation of the transcript. Our approach exemplifies how architectural and functional complexity can emerge from a limited number of distinct building blocks, if molecular-scale programmability, emergent biophysical phenomena, and biochemical activity are coupled to mimic those observed in live cells

Synthetic aperture radar sensitivity to forest changes: A simulations-based study for the Romanian forests

Natural and anthropogenic disturbances pose a significant threat to forest condition. Continuous, reliable and accurate forest monitoring systems are needed to provide earlywarning of potential declines in forest condition. To address that need, state-of-the-art simulationsmodelswere used to evaluate the utility of C-, L- and P-band synthetic aperture radar (SAR) sensors within an integrated Earth-Observation monitoring system for beech, oak and coniferous forests in Romania. The electromagnetic simulations showed differentiated sensitivity to vegetation water content, leaf area index, and forest disturbance depending on SAR wavelength and forest structure. C-band data was largely influenced by foliage volume and therefore may be useful for monitoring defoliation. Changes in water content modulated the C-band signal by b1 dBwhichmay be insufficient for a meaningful retrieval of drought effects on forest. Cband sensitivity to significant clear-cuts was rather low (1.5 dB). More subtle effects such as selective logging or thinning may not be easily detected using C- or L-band data with the longer P-band needed for retrieving small intensity forest disturbances. Overall, the simulations emphasize that additional effort is needed to overcome current limitations arising from the use of a single frequency, acquisition time and geometry by tapping the advantages of dense time series, and by combining acquisitions from active and passive sensors. The simulation results may be applicable to forests outside of Romania since the forests types used in the study have similar morphological characteristics to forests elsewhere in Europe.Romanian National Agency for Scientific Research and Innovation Authorit

„Brain death” donor. Sampling of organs and tissues with their subsequent transplantation

Introduction. Organ and tissue transplantation can save lives or significantly improve the quality of life. In order to organize the activity of taking and transplanting organs and tissues from the donor to the recipient on the territory of the Republic of Moldova, the Law no. 42 of 03.06.2008 "Regarding the transplantation of organs, tissues and human cells" was adopted (published on 25.04.2008 in the Official Gazette, no. 81, art. no. 273, date of entry into force 25.10.2008) and revised Law No. 42 on the transplantation of human organs, tissues and cells of 06.03.2020. Objectives. Steps in sampling of organs and tissues from a "brain death" donor based on the clinical case. Materials and methods. Description of a clinical case about a patient hospitalized in the Municipal Clinical Hospital “Saint Trinity”, Intensive Care department, 2022. Results. A man, 67 years old, was hospitalized in extremely serious condition, from the anamnesis (collected from relatives) - the patient had several comorbidities, including hypertension. The clinical diagnosis: Ischemic stroke. Arterial hypertension grade III very high additional risk. Mixed cardiomyopathy (hypertensive, ischemic, dysmetabolic). Heart failure III NYHA. Despite the complex treatment administered, "brain death" was found, which was confirmed according to the Standardized Clinical Protocol. Following the discussion with the relatives, consent was received for the removal of organs and tissues. The kidney transplant recipient was selected based on HLA typing, Cross-match, pre-existing antibody titer, general condition and lack of contraindications for surgery. 2 kidneys, liver, 2 corneas, 4 vessels were taken from the donor, but one kidney was not transplanted due to the presence of suspicious lesions, after histopathological examination it was established: Atherosclerotic nephropathy. Multiple renal infarcts. Atherosclerosis of the renal artery st. IV, degree II. Conclusions. 1. In the given case, organ removal was possible after the consent of the donor's relatives; 2. Organs, tissues and cells can be taken from the deceased person only if the death has been confirmed according to the criteria established by the Standardized Clinical Protocol (which were confirmed in this case); 3. Following investigations and complex compatibility tests, a patient with liver failure and another with end-stage renal failure were transplanted, other tissues taken (cornea-no.2, vessels-no.4) were processed and stored at the Bank of tissues

Design and preclinical testing of an anti-CD41 CAR T cell for the treatment of acute megakaryoblastic leukaemia

Funding Information: Adrian Bogdan Tigu and Catalin Constantinescu contributed equally to the current manuscript. Catalin Constantinescu is funded by an internal grant of the Iuliu Hatieganu University – School of Doctoral Studies. David Kegyes is funded by an internal grant of the Iuliu Hatieganu University – School of Medicine. Mareike Peters is funded by a national grant of the Romanian Society for Bone Marrow Transplantation. Ciprian Tomuleasa is also supported by a grant awarded by the Romanian National Ministry of Research, Innovation and Digitalization: PN‐III‐P4‐ID‐PCE‐2020‐1118 within PNCDI IV, Projects for Exploratory Medicine; Projects for Exploratory Medicine—PCE 225/2021; as well as a national grant awarded to Young Research Teams (PN‐III‐PI‐1.1‐TE‐2019‐0271 –‘Supporting a team of young researchers to create an independent research program based on the use of Sleeping Beauty protocol f or the development of CAR T Cells – SEATTLE’). Diana Gulei, Diana Cenariu, Adrian Bogdan Tigu, Jon Thor Bergthorsson and Victor Greiff are supported by an international collaborative grant of the European Economic Space between Romania and Iceland 2021–2023: ‘Cooperation strategy for knowledge transfer, internationalization and curricula innovation in the field of research education at the 3rd level of study –AURORA.’ Publisher Copyright: © 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.Acute megakaryoblastic leukaemia (AMkL) is a rare subtype of acute myeloid leukaemia (AML) representing 5% of all reported cases, and frequently diagnosed in children with Down syndrome. Patients diagnosed with AMkL have low overall survival and have poor outcome to treatment, thus novel therapies such as CAR T cell therapy could represent an alternative in treating AMkL. We investigated the effect of a new CAR T cell which targets CD41, a specific surface antigen for M7-AMkL, against an in vitro model for AMkL, DAMI Luc2 cell line. The performed flow cytometry evaluation highlighted a percentage of 93.8% CAR T cells eGFP-positive and a limited acute effect on lowering the target cell population. However, the interaction between effector and target (E:T) cells, at a low ratio, lowered the cell membrane integrity, and reduced the M7-AMkL cell population after 24 h of co-culture, while the cytotoxic effect was not significant in groups with higher E:T ratio. Our findings suggest that the anti-CD41 CAR T cells are efficient for a limited time spawn and the cytotoxic effect is visible in all experimental groups with low E:T ratio.Peer reviewe

Effects of Marine Residue-Derived Fertilizers on Strawberry Growth, Nutrient Content, Fruit Yield and Quality

An outdoor experiment was performed for six months to evaluate the effects of organic fertilizers obtained from marine residual materials on strawberry plants. Three types of organic fertilizers were used, i.e., cod (Gadus morhua) bone powder, common ling (Molva molva) bone powder, and pellets obtained by mixing small cod bone powder and rockweed (Ascophyllum nodosum) residues. A tabletop system for strawberry cultivation was designed, in which two bare-root strawberry plants of cultivar ‘Albion’ were planted in a peat substrate in each pot. Five treatments were applied, i.e., cod bone powder (F1), common ling bone powder (F2), small cod bone powder and rockweed residue pellets (FA), chemical fertilizer (E), and a control (C). The number of leaves and their nutrient content, fruit yield and quality characteristics of the strawberries grown using the organic fertilizers were similar or better than those corresponding to treatments E and C. Organic fertilizers derived from the residues of fish and macroalgae could be a promising alternative to chemical fertilizers in strawberry production

Frequency drift in MR spectroscopy at 3T

Purpose: Heating of gradient coils and passive shim components is a common cause of instability in the B-0 field, especially when gradient intensive sequences are used. The aim of the study was to set a benchmark for typical drift encountered during MR spectroscopy (MRS) to assess the need for real-time field-frequency locking on MRI scanners by comparing field drift data from a large number of sites.Method: A standardized protocol was developed for 80 participating sites using 99 3T MR scanners from 3 major vendors. Phantom water signals were acquired before and after an EPI sequence. The protocol consisted of: minimal preparatory imaging; a short pre-fMRI PRESS; a ten-minute fMRI acquisition; and a long post-fMRI PRESS acquisition. Both pre- and post-fMRI PRESS were non-water suppressed. Real-time frequency stabilization/adjustment was switched off when appropriate. Sixty scanners repeated the protocol for a second dataset. In addition, a three-hour post-fMRI MRS acquisition was performed at one site to observe change of gradient temperature and drift rate. Spectral analysis was performed using MATLAB. Frequency drift in pre-fMRI PRESS data were compared with the first 5:20 minutes and the full 30:00 minutes of data after fMRI. Median (interquartile range) drifts were measured and showed in violin plot. Paired t-tests were performed to compare frequency drift pre- and post-fMRI. A simulated in vivo spectrum was generated using FID-A to visualize the effect of the observed frequency drifts. The simulated spectrum was convolved with the frequency trace for the most extreme cases. Impacts of frequency drifts on NAA and GABA were also simulated as a function of linear drift. Data from the repeated protocol were compared with the corresponding first dataset using Pearson's and intraclass correlation coefficients (ICC).Results: Of the data collected from 99 scanners, 4 were excluded due to various reasons. Thus, data from 95 scanners were ultimately analyzed. For the first 5:20 min (64 transients), median (interquartile range) drift was 0.44 (1.29) Hz before fMRI and 0.83 (1.29) Hz after. This increased to 3.15 (4.02) Hz for the full 30 min (360 transients) run. Average drift rates were 0.29 Hz/min before fMRI and 0.43 Hz/min after. Paired t-tests indicated that drift increased after fMRI, as expected (p &lt; 0.05). Simulated spectra convolved with the frequency drift showed that the intensity of the NAA singlet was reduced by up to 26%, 44 % and 18% for GE, Philips and Siemens scanners after fMRI, respectively. ICCs indicated good agreement between datasets acquired on separate days. The single site long acquisition showed drift rate was reduced to 0.03 Hz/min approximately three hours after fMRI.Discussion: This study analyzed frequency drift data from 95 3T MRI scanners. Median levels of drift were relatively low (5-min average under 1 Hz), but the most extreme cases suffered from higher levels of drift. The extent of drift varied across scanners which both linear and nonlinear drifts were observed.</p