110 research outputs found
Toxicological assessment of nanocrystalline metal alloys with potential applications in the aeronautical field
The development of new candidate alloys with outstanding characteristics for their use in the aeronautical field is one of the main priorities for the sector. In this context, nanocrystaline (nc) alloys are considered relevant materials due to their special features, such as their exceptional physical and mechanical properties. However, another important point that needs to be considered with newly developed alloys is the potential toxicological impact that these materials may have in humans and other living organisms. The aim of this work was to perform a preliminary toxicological evaluation of three nc metal alloys (WCu, WAl and TiAl) in powder form produced by mechanical alloying, applying different in vitro assays, including a mix of W-Cu powders with standard grain size in the experiments to stablish comparisons. The effects of the direct exposure to powder suspensions and/or to their derived leachates were analysed in three model organisms representative of human and environmental exposures (the adenocarcinomic human alveolar basal epithelial cell line A549, the yeast Saccharomyces cerevisiae and the Gram negative bacterium Vibrio fischeri). Altogether, the results obtained provide new insights about the potential harmful effects of the selected nc alloys, showing that, from a toxicological perspective, nc TiAl is the safest candidate in the model organisms and conditions tested.EU Horizon 2020 projects ICARUS (H2020-FETOPEN-2014-2015-RIA, grant agreement N° 713514) and ICARUS-INAS (FETOPEN-03-2018-2019-2020, Grant agreement N° 946174)
Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
Graphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatelets (GN) on the unicellular fungal model Saccharomyces cerevisiae was performed. While cell proliferation was not negatively affected even in the presence of 800âmgâLâ1 of the nanomaterial for 24âhours, oxidative stress was induced at a lower concentration (160âmgâLâ1), after short exposure periods (2 and 4âhours). No DNA damage was observed under a comet assay analysis under the studied conditions. In addition, to pinpoint the molecular mechanisms behind the early oxidative damage induced by GN and to identify possible toxicity pathways, the transcriptome of S. cerevisiae exposed to 160 and 800âmgâLâ1 of GN was studied. Both GN concentrations induced expression changes in a common group of genes (337), many of them related to the fungal response to reduce the nanoparticles toxicity and to maintain cell homeostasis. Also, a high number of genes were only differentially expressed in the GN800 condition (3254), indicating that high GN concentrations can induce severe changes in the physiological state of the yeast.European Unionâs H2020 research and innovation programme under the Marie SkĆodowska-Curie grant agreements N° 691095 and N° 734873; and Junta de Castilla y Leon-FEDER under grants N° BU079U16, and N° UBU-16-B
Influence of three commercial graphene derivatives on the catalytic properties of a lactobacillus plantarum α-l-Rhamnosidase when used as immobilization matrices
The modification of carbon nanomaterials with biological molecules paves the way toward their use in biomedical and biotechnological applications, such as next-generation biocatalytic processes, development of biosensors, implantable electronic devices, or drug delivery. In this study, different commercial graphene derivatives, namely, monolayer graphene oxide (GO), graphene oxide nanocolloids (GOCs), and polycarboxylate-functionalized graphene nanoplatelets (GNs), were compared as biomolecule carrier matrices. Detailed spectroscopic analyses showed that GO and GOC were similar in composition and functional group content and very different from GN, whereas divergent morphological characteristics were observed for each nanomaterial through microscopy analyses. The commercial α-l-rhamnosidase RhaB1 from the probiotic bacterium Lactobacillus plantarum, selected as a model biomolecule for its relevant role in the pharma and food industries, was directly immobilized on the different materials. The binding efficiency and biochemical properties of RhaB1âGO, RhaB1âGOC, and RhaB1âGN composites were analyzed. RhaB1âGO and RhaB1âGOC showed high binding efficiency, whereas the enzyme loading on GN, not tested in previous enzyme immobilization studies, was low. The enzyme showed contrasting changes when immobilized on the different material supports. The effect of pH on the activity of the three RhaB1-immobilized versions was similar to that observed for the free enzyme, whereas the activityâtemperature profiles and the response to the presence of inhibitors varied significantly between the RhaB1 versions. In addition, the apparent Km for the immobilized and soluble enzymes did not change. Finally, the free RhaB1 and the immobilized enzyme in GOC showed the best storage and reutilization stability, keeping most of their initial activity after 8 weeks of storage at 4 °C and 10 reutilization cycles, respectively. This study shows, for the first time, that distinct commercial graphene derivatives can influence differently the catalytic properties of an enzyme during its immobilization.European Unionâs H2020
research and innovation program under the Marie SkĆodowska-Curie grant agreement No. 691095, Ministerio de EconomiaÌ y
Competitividad (CTQ2016-75023-C2-1-P, CTQ2015-70371-
REDT MetDrugs Network), and Junta de Castilla y Leon-
FEDER grants BU076U16, BU079U16 and UBU-11-A
Interaction analysis of commercial graphene oxide nanoparticles with unicellular systems and biomolecules
The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast Saccharomyces cerevisiae and the bacteria Vibrio fischeri to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria Lactobacillus plantarum and the AbG ÎČ-d-glucosidase from Agrobacterium sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, S. cerevisiae (colony forming units, ROS induction, genotoxicity) and V. fischeri (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial.European Unionâs H2020 research and innovation programme under the Marie SkĆodowska-Curie grant agreements NÂș 691095, NÂș 721642 and NÂș 734873; Junta de Castilla y Leon-FEDER under grants NÂș BU079U16, BU291P18 and BU022G18, and Ministerio de EconomĂa y Competitividad CTQ2016-75023-C2-1-P and CTQ2015-70371-REDT MetDrugs Network (Spain
Colonization of electrospun polycaprolactone fibers by relevant pathogenic bacterial strains
Electrospun biodegradable polymers have emerged as promising materials for their applications in several fields, including biomedicine and food industry. For this reason, the susceptibility of these materials to be colonized by different pathogens is a critical issue for public health, and their study can provide future knowledge to develop new strategies against bacterial infections. In this work, the ability of three pathogenic bacterial species (Pseudomonas aeruginosa, Acinetobacter baumannii, and Listeria monocytogenes) to adhere and form biofilm in electrospun polycaprolactone (PCL) microfibrous meshes was investigated. Bacterial attachment was analyzed in meshes with different microstructure, and comparisons with other materials (borosilicate glass and electrospun polylactic acid (PLA)) fibers were assessed. Analysis included colony forming unit (CFU) counts, scanning electron microscopy (SEM), and crystal violet (CV) staining. All the obtained data suggest that PCL meshes, regardless of their microstructure, are highly susceptible to be colonized by the pathogenic relevant bacteria used in this study, so a pretreatment or a functionalization with compounds that present some antimicrobial activity or antibiofilm properties is highly recommended before their application. Moreover, an experiment designed to simulate a chronic wound environment was used to demonstrate the ability of these meshes to detach biofilms from the substratum where they have developed, thus making them promising candidates to be used in wound cleaning and disinfection.European Unionâs H2020 research and innovation
programme under the Marie SkĆodowska-Curie grant agreement
no. 691095 and Junta de Castilla y Leon-FEDER
(projects BU079U16 and BU092U16)
Forward genetics by genome sequencing uncovers the central role of the aspergillus niger goxB locus in hydrogen peroxide induced glucose oxidase expression
Aspergillus niger is an industrially important source for gluconic acid and glucose
oxidase (GOx), a secreted commercially important flavoprotein which catalyses the
oxidation of b-D-glucose by molecular oxygen to D-glucolactone and hydrogen
peroxide. Expression of goxC, the GOx encoding gene and the concomitant two step
conversion of glucose to gluconic acid requires oxygen and the presence of significant
amounts of glucose in the medium and is optimally induced at pH 5.5. The molecular
mechanisms underlying regulation of goxC expression are, however, still enigmatic.
Genetic studies aimed at understanding GOx induction have indicated the involvement
of at least seven complementation groups, for none of which the molecular basis has
been resolved. In this study, a mapping-by-sequencing forward genetics approach was
used to uncover the molecular role of the goxB locus in goxC expression. Using the
Illumina and PacBio sequencing platforms a hybrid high quality draft genome assembly
of laboratory strain N402 was obtained and used as a reference for mapping of genomic
reads obtained from the derivative NW103:goxB mutant strain. The goxB locus encodes
a thioredoxin reductase. A deletion of the encoding gene in the N402 parent strain led
to a high constitutive expression level of the GOx and the lactonase encoding genes
required for the two-step conversion of glucose in gluconic acid and of the catR gene
encoding catalase R. This high constitutive level of expression was observed to be
irrespective of the carbon source and oxidative stress applied. A model clarifying the
role of GoxB in the regulation of the expression of goxC involving hydrogen peroxide as
second messenger is presented
In vitro safety evaluation of rare earth-lean alloys for permanent magnets manufacturing
Due to their exceptional physico-chemical and magnetic characteristics, rare earth (RE) permanent
magnets are applied in multiple critical technologies. However, several environmental and
economic difficulties arising from obtaining RE elements have prompted the search of alternatives
with acceptable magnetic properties but containing a lower percentage of these elements in their
composition. The aim of this work was to perform a preliminary toxicological evaluation of three forms
of newly developed RE-lean alloys (one NdFeTi and two NdFeSi alloys) applying different in vitro
assays, using as a benchmark a commercial NdFeB alloy. Thus, the effects of the direct exposure to
powder suspensions and to their derived leachates were analysed in two model organisms (the A549
human cell line and the yeast Saccharomyces cerevisiae) applying both viability and oxidative stress
assays. Moreover, the impact of the alloy leachates on the bioluminescence of Vibrio fischeri was also
investigated. The obtained data showed that only the direct interaction of the alloys particulates with
the applied organisms resulted in harmful effects, having all the alloys a comparable toxicological
potential to that presented by the reference material in the conditions tested. Altogether, this study
provides new insights about the safety of NdFeTi and NdFeSi alloys.EU Horizon 2020 NOVAMAG project (NMBP 23-2015, Grant Agreement No. 686056) and from the Junta de Castilla y LeĂłn and the European Social Fund-Youth European Initiative Grant UBU-15-A
Carbon nanomaterials with Thymol + Menthol Type V natural deep eutectic solvent: From surface properties to nano-Venturi effect through nanopores
A theoretical study using Density Functional Theory and classical Molecular Dynamics simulations for the
study of carbon nanomaterials in archetypical Menthol + Thymol Type V Natural Deep Eutectic Solvent is
reported. The nanoscopic structure of the representative nanofluid is analyzed considering confinement,
adsorption and solvation effects, as well as consequences on diffusion properties through nano pores.
Different types of nanomaterials were considered such as fullerenes, nanotubes, graphene and nanopores.
The study of nanoscopic properties allowed to analyze the response of the solvent to the presence of the
nanomaterials, taking into account solvent rearrangement and confinement in nanocavities and surfaces.
This response shows liquid structure and mobility consequences, with a sort of nano-Venturi effect
among them. The reported results provide for the first time a characterization of this type of natural solvents as a sustainable platform for the development of carbon â nanomaterials-based technologies.This work was funded by Junta de Castilla y LeĂłn (Spain, project NANOCOMP - BU058P20), European Union H2020 Program (H2020-NMBP-TO-IND-2020-twostage-DIAGONAL-GA- 953152) and Ministerio de Ciencia, InnovaciĂłn y Universidades (Spain, project RTI2018-101987-B-I00). We also acknowledge SCAYLE (SupercomputaciĂłn Castilla y LeĂłn, Spain) for providing supercomputing facilities. The statements made herein are solely the responsibility of the authors
Fate assessment of commercial 2D MoS2 aqueous dispersions at physicochemical and toxicological level
The physicochemical properties and the toxicological potential of commercially available MoS2 nanoparticles with different lateral size and degradation stage were studied in the present research work. To achieve this, the structure and stoichiometry of fresh and old aqueous suspensions of micro-MoS2 and nano-MoS2 was analyzed by Raman, while x-ray photoelectron spectroscopy allowed to identify more quantitatively the nature of the formed oxidized species. A, the toxicological impact of the nanomaterials under analysis was studied using adenocarcinomic human alveolar basal epithelial cells (A549 cells) and the unicellular fungus S. cerevisiae as biological models. Cell viability assays and reactive oxygen species (ROS) determinations demonstrated different toxicity levels depending on the cellular model used and in function of the degradation state of the selected commercial nanoproducts. Both MoS2 nanoparticle types induced sublethal damage on the A549 cells though the increase of intracellular ROS levels, while comparable concentrations reduced the viability of yeast cells. In addition, the old MoS2 nanoparticles suspensions exhibited a higher toxicity for both human and yeast cells than the fresh ones. Our findings demonstrate that the fate assessment of nanomaterials is a critical aspect to increase the understanding on their characteristics and on their potential impact on biological systems along their life cycle
Assessment of Physico-Chemical and Toxicological Properties of Commercial 2D Boron Nitride Nanopowder and Nanoplatelets
Boron nitride (BN) nanomaterials have been increasingly explored for potential applications in chemistry and biology fields (e.g., biomedical, pharmaceutical, and energy industries) due to their unique physico-chemical properties. However, their safe utilization requires a profound knowledge on their potential toxicological and environmental impact. To date, BN nanoparticles have been considered to have a high biocompatibility degree, but in some cases, contradictory results on their potential toxicity have been reported. Therefore, in the present study, we assessed two commercial 2D BN samples, namely BN-nanopowder (BN-PW) and BN-nanoplatelet (BN-PL), with the objective to identify whether distinct physico-chemical features may have an influence on the biological responses of exposed cellular models. Morphological, structural, and composition analyses showed that the most remarkable difference between both commercial samples was the diameter of their disk-like shape, which was of 200â300 nm for BN-PL and 100â150 nm for BN-PW. Their potential toxicity was investigated using adenocarcinomic human alveolar basal epithelial cells (A549 cells) and the unicellular fungus Saccharomycescerevisiae, as human and environmental eukaryotic models respectively, employing in vitro assays. In both cases, cellular viability assays and reactive oxygen species (ROS) determinations where performed. The impact of the selected nanomaterials in the viability of both unicellular models was very low, with only a slight reduction of S. cerevisiae colony forming units being observed after a long exposure period (24 h) to high concentrations (800 mg/L) of both nanomaterials. Similarly, BN-PW and BN-PL showed a low capacity to induce the formation of reactive oxygen species in the studied conditions. Even at the highest concentration and exposure times, no major cytotoxicity indicators were observed in human cells and yeast. The results obtained in the present study provide novel insights into the safety of 2D BN nanomaterials, indicating no significant differences in the toxicological potential of similar commercial products with a distinct lateral size, which showed to be safe products in the concentrations and exposure conditions tested
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