Avaliação do resultado do bypass gastrojejunal em y-roux laboroscópico em pacientes obesos mórbidos portadores de diabetes tipo dois.

Trabalho de Conclusão de Curso - Universidade Federal de Santa Catarina. Curso de Medicina. Dapartamento de Clínica Cirúrgica

Технологические решения для строительства разведочной вертикальной скважины глубиной 2950 метров на нефтяном месторождении (Томская область)

Объектом исследования является разведочная вертикальная скважина глубиной 2950 метров на нефтегазовом месторождении. Целью работы является – спроектировать технологические решения для бурения вертикальной разведочной скважины глубиной 2950 м.The object of the study is a vertical exploration well with a depth of 2950 meters in an oil and gas field. The aim of the work is to design technological solutions for drilling a vertical exploration well with a depth of 2950 m

Melanotic oncocytic metaplasia of the nasopharynx as a benign mimicker of malignant melanoma: a case report

<p>Abstract</p> <p>Introduction</p> <p>Melanotic variant of oncocytic metaplasia of the nasopharynx is an extremely rare condition.</p> <p>Case report</p> <p>A 73-year-old Japanese man presented with nasal congestion and chill. Nasoscopic examination revealed multiple black nodules around the bilateral torus tubarius. The nodules were biopsied to determine the histology. The clinical differential diagnosis was malignant melanoma or hemangioma. Microscopically, there were oncocytic plump cells with abundant brown pigmented granules showing glandular pattern. No significant atypia was found. The pigment was positive for Fontana-Masson staining, and negative for Berlin blue staining, showing that it was melanin pigment. Immunohistochemically, S100-positive HMB45-negative dendritic cells were also found.</p> <p>Conclusion</p> <p>Such a pigmented variant of benign oncocytic lesion is very rare, and only 15 cases have been reported in the English literature. As a benign mimicker of malignant melanoma, melanocytic oncocytic metaplasia should be always taken into consideration in the clinical setting.</p

Roles of Macrophages in Advanced Liver Fibrosis, Identified Using a Newly Established Mouse Model of Diet-Induced Non-Alcoholic Steatohepatitis

Macrophages play critical roles in the pathogenesis of non-alcoholic steatohepatitis (NASH). However, it is unclear which macrophage subsets are critically involved in the development of inflammation and fibrosis in NASH. In TSNO mice fed a high-fat/cholesterol/cholate-based diet, which exhibit advanced liver fibrosis that mimics human NASH, we found that Kupffer cells (KCs) were less abundant and recruited macrophages were more abundant, forming hepatic crown-like structures (hCLS) in the liver. The recruited macrophages comprised two subsets: CD11c+/Ly6C−and CD11c− /Ly6C+ cells. CD11c+ cells were present in a mesh-like pattern around the lipid droplets, constituting the hCLS. In addition, CD11c+ cells colocalized with collagen fibers, suggesting that this subset of recruited macrophages might promote advanced liver fibrosis. In contrast, Ly6C+cells were present in doughnut-like inflammatory lesions, with a lipid droplet in the center. Finally, RNA sequence analysis indicates that CD11c+/Ly6C− cells promote liver fibrosis and hepatic stellate cell (HSC) activation, whereas CD11c−/Ly6C+ cells are a macrophage subset that play an anti-inflammatory role and promote tissue repair in NASH. Taken together, our data revealed changes in liver macrophage subsets during the development of NASH and shed light on the roles of the recruited macrophages in the pathogenesis of advanced fibrosis in NASH

Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors

We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film using a UV-curable adhesive and photolithography. The package was processed at room temperature to avoid denaturation of the glucose oxidase. The glucose oxidase solution was encapsulated and unsealed. Glucose sensing was demonstrated using standard amperometric detection at glucose concentrations between 0.1 and 100 mM, which covers the glucose concentration range of diabetic patients. Our proposed Parylene encapsulation and UV-adhesive cover form a liquid phase glucose-oxidase package that has the advantages of room temperature processing and direct liquid encapsulation of a small volume solution without use of conventional solidifying chemicals

Progress in Outreach of ERI’s Research Results Using Interactive Rich Contents Display System

We have developed an interactive display system operated by touch panel as one of the best ways to reach out the visitors by showing the outcomes of the Earthquake Research Institute (ERI). In this paper, we report some of the contents and the benefits of the interactive display. Research results are summarized as movies or high resolution graphics which we call “rich-contents”. We provide 65 and 45 inch touch panels connected to PCs which run Vizlmpress envision software developed by SGI, Japan. The rich-contents consist of three parts: 1) Introduction of Earthquakes and Volcanoes, 2) Outline of ERI, and 3) Research Highlights. Part 1 provides an animation of asperity, historical earthquake prints (Namazu-e) and disaster pictures, photos from "The Great Earthquake in Japan 1891" taken by J. Milne and W.K. Burton, and a high resolution map of the world seismicity. Part 2 includes a general description and history of ERI, photos of researchers, and ERI booklets such as brochure, annual reports or newsletters. Part 3 presents videos of observation sceneries from the top of the active volcanoes or from the cruise for settling ocean bottom seismometers. Computer simulations of strong ground motions, Tsunamis, mantle convection, and seismic shaking of builidngs are also included. One of the most enjoyable contents is the 3-dimensional seismicity map around Japan, from which you can rotate Japan and get to know how the oceanic plates subduct beneath Japan. This system was demonstrated at the exhibition booths of the Japan Geoscience Union mettings in 2007 and 2008, and Cities on Volcanoes 5 conference in 2007, and got the attention of visitors including high school students. The key factors of this captivation, in addition to the attractive and dynamic research results themselves, may be brought from 1) the quick response of the touch panel as well as the smooth zoom-in and zoom-out, and 2) a close distance between the audience and the presenter so that the presenter can be viewed as a part of the screen

Nitrogen oxide cycle regulates nitric oxide levels and bacterial cell signaling

Nitric oxide (NO) signaling controls various metabolic pathways in bacteria and higher eukaryotes. Cellular enzymes synthesize and detoxify NO; however, a mechanism that controls its cellular homeostasis has not been identified. Here, we found a nitrogen oxide cycle involving nitrate reductase (Nar) and the NO dioxygenase flavohemoglobin (Fhb), that facilitate inter-conversion of nitrate, nitrite, and NO in the actinobacterium Streptomyces coelicolor. This cycle regulates cellular NO levels, bacterial antibiotic production, and morphological differentiation. NO down-regulates Nar and up-regulates Fhb gene expression via the NO-dependent transcriptional factors DevSR and NsrR, respectively, which are involved in the auto-regulation mechanism of intracellular NO levels. Nitrite generated by the NO cycles induces gene expression in neighboring cells, indicating an additional role of the cycle as a producer of a transmittable inter-cellular communication molecule

ネツ ルミネッセンス 2ジゲン ヨミトリ システム

熱ルミネッセンス[TL]2次元読み取りシステムを開発した。このシステムを使った応用として, 人工のTL-sheet(BaSO_4 : Eu)を用いて高エネルギー宇宙線シャワーの検出を行なった例と, 天然の試料(鍾乳石, 花崗岩)のTL空間分布を測定した例を示す.A spatial distribution read-out system of thermoluminescence [TL] is developed. This system is applied to artificial TL-sheets (BaSO_4 : Eu) for detecting high energy cosmic ray showers and to inhomogeneous materials for measuring natural TL spatial distributions of stalactite and Mannari-granite

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection