Técnicas y estrategias en la solución de conflictos escolares de la Institución Educativa Ramón Munera Lopera

Tesis de la Sede Bello Uniminuto-Seccional BelloEl trabajo que se presenta a continuación dará cuenta de la práctica profesional, desde el campo ocupacional de la Psicología social, en especial la psicología educativa. Esta práctica se realizó en la Institución Educativa Ramón Múnera Lopera, ubicada en la comuna 3, Sector de Manrique en la ciudad de Medellín; la misma se llevó desde marzo del año 2014 hasta octubre del mismo año.Corporación Universitaria Minuto de Dio

Singlet-doublet Dirac fermion dark matter from Peccei-Quinn symmetry

Weakly Interacting Massive Particles (WIMPs) and axions are arguably the most compelling dark matter (DM) candidates in the literature. Here, we consider a model where the PQ symmetry solves the strong CP problem, generates radiatively Dirac neutrino masses, and gives origin to multicomponent dark sector. Specifically, scotogenic Dirac neutrino masses arise at one-loop level. The lightest fermionic mediator acts as the second DM candidate due to a residual $Z_2$ symmetry resulting from the PQ symmetry breaking. The WIMP DM component resembles the well-known singlet-doublet fermion DM. While the lower WIMP dark mass region is usually excluded, our model reopens that portion of the parameter space (for DM masses below $\lesssim 100$ GeV). Therefore, we perform a phenomenological analysis that addresses the constraints from direct searches of DM, neutrino oscillation data, and charged lepton flavor violating (LFV) processes. The model can be tested in future facilities where DM annihilation into SM particles is searched for by neutrino telescopes.Comment: 27 pages, 6 figures and 2 table

Architecture design and prototype implementation that integrate the processes of the Laboratory of Geotechnics and Construction materials

El Laboratorio de Geotecnia y Materiales de Construcción, lleva a cabo sus procesos técnicos de ensayo, aplicando una serie de disposiciones técnicas documentadas y fundamentadas en normas técnicas reconocidas que son aplicadas eficazmente por el personal técnico y de apoyo. Por lineamientos de Calidad estos procesos deben seguir la norma ISO 17025. Por consiguiente, el laboratorio requiere integrar, dentro de un sistema de información, los procesos de recepción de muestras, manejo de inventarios, control de equipos, almacenamiento y procesamiento de datos, elaboración de cálculos e informes finales para así lograr un acceso, búsqueda y procesamiento de una forma más rápida. La propuesta central es diseñar la arquitectura general del sistema que permita implementar los procesos del laboratorio e implementar un prototipo que contenga el módulo de creación de formularios dinámicos. Estos formularios permitirían gestionar los datos de los formatos que actualmente utiliza el laboratorio. Actualmente los formularios se implementan en hojas de cálculo (tipo Excel) con las que se manejan datos de ensayo, recepción de muestra, órdenes internas, entre otros. Lo que se espera es que el administrador del laboratorio pueda subir los formularios a la plataforma sin necesidad de un desarrollador. Este módulo se diseñará bajo una de las arquitecturas propuestas a manera de guía funcional para el desarrollo de la aplicación. La planeación y desarrollo del proyecto se llevó a cabo con una metodología en espiral basada en prototipos. Esta metodología de desarrollo tiene un alto componente de diseño ya que en cada ciclo se debe: determinar objetivos, análisis de riesgos, planificación, y por último desarrollo y prueba. Se basa en prototipos puesto que se implementa cada módulo, se prueba y luego se integra en un prototipo funcional, principalmente enfocado en el módulo formularios dinámicos y las entradas de estos.The Laboratory of Geotechnics and Construction Materials carries out its technical testing processes, applying a series of technical provisions documented and based on recognized technical standards that are effectively applied by technical and support staff. In addition, the quality these processes must follow the ISO 17025 standard. Therefore, the laboratory requires an information system that integrate the processes of sample reception, inventory management, equipment control, storage and data processing, preparation of calculations and final reports in order to achieve access, search and processing in a faster way. The central proposal is to design the complete architecture of the system that allows to implement some of the processes of the laboratory within a prototype that contains the module of dynamic forms creation. These forms would allow to manage the data of the forms actually used by the laboratory. Currently the forms are implemented in spreadsheets (Excel type) which data of the test is handled, sample reception, internal orders, among others. What is expected is that the laboratory administrator can upload the forms to the platform without the need of a developer. This module will be designed under one of the proposed architectures as a functional guide for the development of the application. The planning and development of the project was carried out with a spiral methodology based on prototypes. This development methodology has a high design component since each cycle must: determine objectives, risk analysis, planning, and finally development and testing. It is based on prototypes since each module is implemented, tested and then integrated into a functional prototype, mainly focused on the dynamic forms module and the inputs of thes

Multi-monoubiquitylation controls VASP-mediated actin dynamics Icon for The Forest of Biologists

The actin cytoskeleton performs multiple cellular functions, and as such, actin polymerization must be tightly regulated. We previously demonstrated that reversible, non-degradative ubiquitylation regulates the function of the actin polymerase VASP in developing neurons. However, the underlying mechanism of how ubiquitylation impacts VASP activity was unknown. Here, we show that mimicking multi-monoubiquitylation of VASP at K240 and K286 negatively regulates VASP interactions with actin. Using in vitro biochemical assays, we demonstrate the reduced ability of multi-monoubiquitylated VASP to bind, bundle, and elongate actin filaments. However, multi-monoubiquitylated VASP maintained the ability to bind and protect barbed ends from capping protein. Finally, we demonstrate the electroporation of recombinant multi-monoubiquitylated VASP protein altered cell spreading morphology. Collectively, these results suggest a mechanism in which ubiquitylation controls VASP-mediated actin dynamics

Profilin-1 Serves as a Gatekeeper for Actin Assembly by Arp2/3-Dependent and -Independent Pathways

Cells contain multiple F-actin assembly pathways including the Arp2/3 complex, formins, and Ena/VASP, which have largely been analyzed separately. They collectively generate the bulk of F-actin from a common pool of G-actin; however, the interplay/competition between these pathways remains poorly understood. Using fibroblast lines derived from an Arpc2 conditional knockout mouse, we established matched-pair cells with and without the Arp2/3 complex. Arpc2−/− cells lack lamellipodia and migrate slower than WT cells, but have F-actin levels indistinguishable from controls. Actin assembly in Arpc2−/− cells was resistant to cytochalasin-D and was highly dependent on profilin-1 and Ena/VASP, but not formins. Profilin-1 depletion in WT cells increased F-actin and Arp2/3 complex in lamellipodia. Conversely, addition of exogenous profilin-1 inhibited Arp2/3 complex actin nucleation in vitro and in vivo. These observations suggest that antagonism of the Arp2/3 complex by profilin-1 in cells maintains actin homeostasis by balancing Arp2/3 complex-dependent and independent actin assembly pathways

Pth4, an ancient parathyroid hormone lost in eutherian mammals, reveals a new brain-to-bone signaling pathway

Regulation of bone development, growth, and remodeling traditionally has been thought to depend on endocrine and autocrine/paracrine modulators. Recently, however, brain-derived signals have emerged as key regulators of bone metabolism, although their mechanisms of action have been poorly understood. We reveal the existence of an ancient parathyroid hormone (Pth)4 in zebrafish that was secondarily lost in the eutherian mammals' lineage, including humans, and that is specifically expressed in neurons of the hypothalamus and appears to be a central neural regulator of bone development and mineral homeostasis. Transgenic fish lines enabled mapping of axonal projections leading from the hypothalamus to the brainstem and spinal cord. Targeted laser ablation demonstrated an essential role for of pth4-expressing neurons in larval bone mineralization. Moreover, we show that Runx2 is a direct regulator of pth4 expression and that Pth4 can activate cAMP signaling mediated by Pth receptors. Finally, gain-of-function experiments show that Pth4 can alter calcium/phosphorus levels and affect expression of genes involved in phosphate homeostasis. Based on our discovery and characterization of Pth4, we propose a model for evolution of bone homeostasis in the context of the vertebrate transition from an aquatic to a terrestrial lifestyle.Spanish Economy and Competitiveness Ministry Project [ALG2011-23581, AGL2014-52473R]; Portuguese Foundation for Science and Technology [PTDC/BIA-ANM/4225/2012-phos-fate]; U. S. National Institutes of Health/Office of the Director Grant [R01OD011116, R01 RR020833]; Generalitat de Catalunya [SGR2014-290]; Spanish Economy and Competitiveness Ministry [BFU2010-14875]; Science and Innovation Ministry [AGL2010-22247-C03-01]; Campus do Mar Ph.D. grant; Xunta de Galicia (Santiago, Spain) [AGL2014-52473R]info:eu-repo/semantics/publishedVersio

First Results of the 140^{140}Ce(n,γ)141^{141}Ce Cross-Section Measurement at n_TOF

An accurate measurement of the $^{140}$Ce(n,γ) energy-dependent cross-section was performed at the n_TOF facility at CERN. This cross-section is of great importance because it represents a bottleneck for the s-process nucleosynthesis and determines to a large extent the cerium abundance in stars. The measurement was motivated by the significant difference between the cerium abundance measured in globular clusters and the value predicted by theoretical stellar models. This discrepancy can be ascribed to an overestimation of the $^{140}$Ce capture cross-section due to a lack of accurate nuclear data. For this measurement, we used a sample of cerium oxide enriched in $^{140}$Ce to 99.4%. The experimental apparatus consisted of four deuterated benzene liquid scintillator detectors, which allowed us to overcome the difficulties present in the previous measurements, thanks to their very low neutron sensitivity. The accurate analysis of the p-wave resonances and the calculation of their average parameters are fundamental to improve the evaluation of the $^{140}$Ce Maxwellian-averaged cross-section

First Results of the 140^{140}Ce(n,γ)141^{141}Ce Cross-Section Measurement at n_TOF

An accurate measurement of the $^{140}$Ce(n,γ) energy-dependent cross-section was performed at the n_TOF facility at CERN. This cross-section is of great importance because it represents a bottleneck for the s-process nucleosynthesis and determines to a large extent the cerium abundance in stars. The measurement was motivated by the significant difference between the cerium abundance measured in globular clusters and the value predicted by theoretical stellar models. This discrepancy can be ascribed to an overestimation of the $^{140}$Ce capture cross-section due to a lack of accurate nuclear data. For this measurement, we used a sample of cerium oxide enriched in $^{140}$Ce to 99.4%. The experimental apparatus consisted of four deuterated benzene liquid scintillator detectors, which allowed us to overcome the difficulties present in the previous measurements, thanks to their very low neutron sensitivity. The accurate analysis of the p-wave resonances and the calculation of their average parameters are fundamental to improve the evaluation of the $^{140}$Ce Maxwellian-averaged cross-section

Prospective individual patient data meta-analysis of two randomized trials on convalescent plasma for COVID-19 outpatients

Data on convalescent plasma (CP) treatment in COVID-19 outpatients are scarce. We aimed to assess whether CP administered during the first week of symptoms reduced the disease progression or risk of hospitalization of outpatients. Two multicenter, double-blind randomized trials (NCT04621123, NCT04589949) were merged with data pooling starting when = 50 years and symptomatic for <= 7days were included. The intervention consisted of 200-300mL of CP with a predefined minimum level of antibodies. Primary endpoints were a 5-point disease severity scale and a composite of hospitalization or death by 28 days. Amongst the 797 patients included, 390 received CP and 392 placebo; they had a median age of 58 years, 1 comorbidity, 5 days symptoms and 93% had negative IgG antibody-test. Seventy-four patients were hospitalized, 6 required mechanical ventilation and 3 died. The odds ratio (OR) of CP for improved disease severity scale was 0.936 (credible interval (CI) 0.667-1.311); OR for hospitalization or death was 0.919 (CI 0.592-1.416). CP effect on hospital admission or death was largest in patients with <= 5 days of symptoms (OR 0.658, 95%CI 0.394-1.085). CP did not decrease the time to full symptom resolution